Strain Name:

FVB-Tg(Ttr-Igf1)1Sykr/J

Stock Number:

012662

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
This transgenic mutant mouse strain may be useful in studies of growth and metabolism.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names FVB-Tg(Ttr-Igf1)1Syak/J    (Changed: 21-JUN-10 )
Type Coisogenic; Gene Trap; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating Investigator Shoshana Yakar,   Mount Sinai School of Medicine

Description
These "HIT" transgenic mice overexpress rat IGF1 specifically in the liver, with normal tissue IGF-I but have increased serum IGF-I levels. Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator reports that there is no difference between the phenotype exhibited by homozygotes compared to hemizygotes.

Development
A transgenic construct containing the rat insulin-like growth factor 1, Igf1, under the control of the rat Ttr, transthyretin, promoter was injected into FVB/N fertilized eggs. Founder line 1 was subsequently established. The mice were then backcrossed to FVB/N for 6 generations. Upon arrival at The Jackson Laboratory the mice were crossed with FVB/NJ (Stock No. 001800) once to establish the colony.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Igf1
002500   C57BL/6J-Tg(WapIgf1)39Dlr/J
003078   FVB.Cg-Tg(WapIgf1)39Dlr/J
View Strains carrying other alleles of Igf1     (2 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(Ttr-Igf1)1Sykr/0

        FVB/N-Tg(Ttr-Igf1)1Sykr
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • mice exhibit normal fasting glucose and glucose clearance   (MGI Ref ID J:157350)
    • decreased circulating insulin level
      • mice exhibit a tend towards reduced serum insulin levels compared with wild-type mice   (MGI Ref ID J:157350)
    • increased circulating insulin-like growth factor I level   (MGI Ref ID J:157350)
  • adipose tissue phenotype
  • decreased gonadal fat pad weight   (MGI Ref ID J:157350)
  • decreased percent body fat
    • at 4 and 8 weeks but not 16 weeks   (MGI Ref ID J:157350)
  • decreased total body fat amount
    • at 4 ad 8 weeks in female mice   (MGI Ref ID J:157350)
  • reproductive system phenotype
  • abnormal superovulation
    • following hormone treatment, more eggs are released compared to in similarly treated wild-type mice   (MGI Ref ID J:157350)
  • growth/size/body phenotype
  • decreased percent body fat
    • at 4 and 8 weeks but not 16 weeks   (MGI Ref ID J:157350)
  • decreased total body fat amount
    • at 4 ad 8 weeks in female mice   (MGI Ref ID J:157350)
  • increased body length
    • at 4 weeks of age   (MGI Ref ID J:157350)
  • increased body weight
    • starting at 2 weeks and persistent throughout 16 weeks   (MGI Ref ID J:157350)
  • endocrine/exocrine gland phenotype
  • abnormal branching of the mammary ductal tree
    • mice exhibit increased ductal branching compared to in wild-type mice   (MGI Ref ID J:157350)
  • hematopoietic system phenotype
  • increased spleen weight
  • renal/urinary system phenotype
  • increased kidney weight
  • liver/biliary system phenotype
  • increased liver weight
  • immune system phenotype
  • increased spleen weight
  • integument phenotype
  • abnormal branching of the mammary ductal tree
    • mice exhibit increased ductal branching compared to in wild-type mice   (MGI Ref ID J:157350)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Genes Regulating Growth and Proliferation

Metabolism Research

Research Tools
Metabolism Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Ttr-Igf1)1Sykr
Allele Name transgene insertion 1, Shoshana Yakar
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) HIT;
Strain of OriginFVB/N
Expressed Gene Igf1, insulin-like growth factor 1, rat
Promoter Ttr, transthyretin, rat
Molecular Note The transgene contains the rat insulin-like growth factor 1 (Igf1) under the control of the rat Ttr (transthyretin) promoter. Line 1 was created. [MGI Ref ID J:157350]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Ttr-Igf1)1Syak, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Wu Y; Sun H; Yakar S; LeRoith D. 2009. Elevated levels of insulin-like growth factor (IGF)-I in serum rescue the severe growth retardation of IGF-I null mice. Endocrinology 150(9):4395-403. [PubMed: 19497975]  [MGI Ref ID J:157350]

Additional References

Tg(Ttr-Igf1)1Sykr related

Cannata D; Lann D; Wu Y; Elis S; Sun H; Yakar S; Lazzarino DA; Wood TL; Leroith D. 2010. Elevated circulating IGF-I promotes mammary gland development and proliferation. Endocrinology 151(12):5751-61. [PubMed: 20926579]  [MGI Ref ID J:169499]

Elis S; Courtland HW; Wu Y; Rosen CJ; Sun H; Jepsen KJ; Majeska RJ; Yakar S. 2010. Elevated serum levels of IGF-1 are sufficient to establish normal body size and skeletal properties even in the absence of tissue IGF-1. J Bone Miner Res 25(6):1257-66. [PubMed: 20200935]  [MGI Ref ID J:169833]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as hemizygotes or homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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