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| Exon 3 of the Mstn (myostatin) gene is flanked by loxP sites in this targeted mutation strain. Homozygotes for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in studies of muscle growth and maintenance. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N?pN1
Generation DefinitionsDonating Investigator Jeffery D. Molkentin, Children's Hospital Medical Center Description
Exon 3 of the Mstn (myostatin) gene is flanked by loxP sites in this targeted mutation strain. Homozygotes for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in studies of muscle growth and maintenance.For example, when crossed to a strain expressing tamoxifen-inducible Cre recombinase in most tissues (see Stock No. 004682), this mutant mouse strain may be useful in studies of muscle development.
Development
A loxP-flanked neomycin resistance cassette was introduced to intron 3 and a third loxP site was placed in intron 2 of the targeted gene using AB1 129S7/SvEvBrd-Hprt1+-derived embryonic stem (ES) cells. The neomycin cassette was excised, leaving the floxed exon 3. This strain was backcrossed minimally to C57BL/6 by the donating laboratory.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Mstn
009345 C57BL/6J-Mstnlean/J View Strains carrying other alleles of Mstn (1 strain)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Muscle Hypertrophy; MSLHP (MSTN)
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Mstntm1Swel/Mstntm1Swel Tg(CAG-cre/Esr1*)5Amc/?
involves: 129S7/SvEvBrd-Hprt1 * C57BL/6 * CBA (conditional)
- muscle phenotype
- increased skeletal muscle fiber diameter
- the mean cross-sectional area of an individual quadriceps fibers in tamoxifen treated mice is 42% greater than in treated controls (MGI Ref ID J:121233)
- increased skeletal muscle mass
- after 3 months of tamoxifen treatment muscle mass is increased by 25% compared to untreated mice and treated wild-type mice in the gastrocnemius and quadriceps of males and females (MGI Ref ID J:121233)
This mouse can be used to support research in many areas including:
Research Tools
Cre-lox System
loxP-flanked Sequences
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Mstntm1Swel | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Stephen Welle | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | Mstnf; Mstn[f]; | ||
| Mutation Made By | Stephen Welle, University of Rochester Medical Center | ||
| Strain of Origin | 129S7/SvEvBrd-Hprt<+> | ||
| Gene Symbol and Name | Mstn, myostatin | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | Cmpt; GDF8; Gdf8; MSLHP; compact; growth differentiation factor 8; | ||
| Molecular Note | LoxP sites were inserted to flank exon 3, which encodes the entire active portion of the peptide. [MGI Ref ID J:106054] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Mstntm1Swel, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Welle S; Bhatt K; Pinkert CA. 2006. Myofibrillar protein synthesis in myostatin-deficient mice. Am J Physiol Endocrinol Metab 290(3):E409-15. [PubMed: 16219664] [MGI Ref ID J:106054]
Additional References
Mstntm1Swel relatedBurgess K; Xu T; Brown R; Han B; Welle S. 2011. Effect of myostatin depletion on weight gain, hyperglycemia, and hepatic steatosis during five months of high-fat feeding in mice. PLoS One 6(2):e17090. [PubMed: 21390326] [MGI Ref ID J:171042]
Heineke J; Auger-Messier M; Xu J; Sargent M; York A; Welle S; Molkentin JD. 2010. Genetic deletion of myostatin from the heart prevents skeletal muscle atrophy in heart failure. Circulation 121(3):419-25. [PubMed: 20065166] [MGI Ref ID J:168444]
Personius KE; Jayaram A; Krull D; Brown R; Xu T; Han B; Burgess K; Storey C; Shah B; Tawil R; Welle S. 2010. Grip force, EDL contractile properties, and voluntary wheel running after postdevelopmental myostatin depletion in mice. J Appl Physiol 109(3):886-94. [PubMed: 20595537] [MGI Ref ID J:185924]
Welle S; Bhatt K; Pinkert CA; Tawil R; Thornton CA. 2007. Muscle growth after postdevelopmental myostatin gene knockout. Am J Physiol Endocrinol Metab 292(4):E985-91. [PubMed: 17148752] [MGI Ref ID J:121233]
Welle S; Burgess K; Thornton CA; Tawil R. 2009. Relation between extent of myostatin depletion and muscle growth in mature mice. Am J Physiol Endocrinol Metab :. [PubMed: 19654287] [MGI Ref ID J:159582]
Welle S; Cardillo A; Zanche M; Tawil R. 2009. Skeletal muscle gene expression after myostatin knockout in mature mice. Physiol Genomics 38(3):342-50. [PubMed: 19509079] [MGI Ref ID J:157964]
Welle S; Mehta S; Burgess K. 2011. Effect of postdevelopmental myostatin depletion on myofibrillar protein metabolism. Am J Physiol Endocrinol Metab 300(6):E993-E1001. [PubMed: 21406613] [MGI Ref ID J:172300]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintained as a live colony, heterozygotes or homozygotes may be bred.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Tel: 1-800-422-6423 or 1-207-288-5845
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For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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