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| This Cck-IRES-Cre knockin allele harbors an internal ribosome entry site and Cre recombinase in the 3' UTR of the cholecystokinin locus (Cck). As such, Cre recombinase expression is directed to Cck-expressing cells by the endogenous promoter/enhancer elements of the cholecystokinin locus. The donating investigator reports Cre recombinase activity is observed in cholecystokinin positive neurons. | |||||||||||||||||||
- Description
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Description
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 27-OCT-11 Species laboratory mouse Generation F?+F2 (30-NOV-11)
Generation DefinitionsDonating Investigator Z. Josh Huang, Cold Spring Harbor Laboratory Description
The Cck-IRES-Cre allele harbors an internal ribosome entry site and Cre recombinase in the 3' UTR of the cholecystokinin locus (Cck). As such, cre expression is directed by the endogenous Cck promoter/enhancer elements. The donating investigator reports that homozygous mice are viable and fertile. When Cck-IRES-Cre mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the Cck-expressing cells in the offspring.The donating investigator reports Cre recombinase activity is specific and efficient (largely recapitulates the endogenous Cck expression pattern with highly efficient recombination). They report Cre recombinase activity is observed in cholecystokinin positive neurons (interneurons) of the cortex. Characterization of cre expression in tissues other than brain is not yet available.
For characterization information, see images at the Allen Institute for Brain Science website (Cck-IRES-Cre images).
Development
A targeting vector was designed to insert an internal ribosome entry site (IRES), a Cre recombinase sequence, a polyA sequence, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the cholecystokinin locus (Cck). This construct was electroporated into (C57BL/6 x 129S4Sv/Jae)-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric males were bred with Actin-FLPe females (on a C57BL/6 congenic background (N10); see Stock No. 005703) to achieve germline transmission and to remove the neo selection cassette. The resulting pups harboring the cre targeted mutation were selected. These Cck-IRES-Cre mice were subsequently bred together for several generations (and the FLPe transgene was removed) prior to arrival at The Jackson Laboratory Repository. Upon arrival, mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
NIH Neuroscience Blueprint cre strains from CSHL
010774 B6(Cg)-Calb2tm1(cre)Zjh/J 013730 B6(Cg)-Calb2tm2.1(cre/ERT2)Zjh/J 012704 B6(Cg)-Crhtm1(cre)Zjh/J 010705 B6(Cg)-Dlx5tm1(cre/ERT2)Zjh/J 013048 B6(Cg)-Etv1tm1.1(cre/ERT2)Zjh/J 010776 B6(Cg)-Lhx6tm1(cre/ERT2)Zjh/J 010777 B6(Cg)-Pvalbtm1(cre/ERT2)Zjh/J 010708 B6(Cg)-Ssttm1(cre/ERT2)Zjh/J 014541 B6;129S-Nos1tm1.1(cre/ERT2)Zjh/J 014551 B6;129S4-Dlx1tm1(cre/ERT2)Zjh/J 012710 STOCK Ccktm2.1(cre/ERT2)Zjh/J 010910 STOCK Corttm1(cre)Zjh/J 010702 STOCK Gad2tm1(cre/ERT2)Zjh/J 010802 STOCK Gad2tm2(cre)Zjh/J 014552 STOCK Nkx2-1tm1.1(cre/ERT2)Zjh/J 013044 STOCK Ssttm2.1(cre)Zjh/J 010908 STOCK Viptm1(cre)Zjh/J View NIH Neuroscience Blueprint cre strains from CSHL (17 strains)
017710 B6.129-Ccktm1Lcs/J 011086 C57BL/6-Tg(Cck-cre)CKres/J 012710 STOCK Ccktm2.1(cre/ERT2)Zjh/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Ccktm1.1(cre)Zjh/Cck+
involves: C57BL/6 * SJL
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:151755)
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Ccktm1.1(cre)Zjh | ||
|---|---|---|---|
| Allele Name | targeted mutation 1.1, Z Josh Huang | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Cck-IRES-Cre; | ||
| Mutation Made By | Z. Josh Huang, Cold Spring Harbor Laboratory | ||
| Site of Expression | Cre recombinase activity is observed in cholecystokinin positive neurons (interneurons) of the cortex. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Driver Note | Cck | ||
| General Note | ES cells = C57BL/6;129 | ||
| Molecular Note | A targeting vector was designed to insert an internal ribosome entry site (IRES), a cre recombinase sequence, a polyA sequence, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the cholecystokinin locus (Cck). This construct was electroporated into C57BL/6;129 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric males were bred with Actin-FLPe females (on a C57BL/6 congenic background) to achieve germline transmission and to remove the neo selection cassette. The resulting pups harboring the cre targeted mutation were selected. These Cck-IRES-Cre mice were subsequently bred together for several generations (and the FLPe transgene was removed). [MGI Ref ID J:151755] | ||
| Gene Symbol and Name | Cck, cholecystokinin | ||
| Chromosome | 9 | ||
Genotyping
Genotyping Information
Genotyping Protocols
Ccktm1.1(cre)Zjh, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
NIH Neuroscience Blueprint Cre Driver Network. 2009. Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network MGI Direct Data Submission :. [MGI Ref ID J:151755]
Taniguchi H; He M; Wu P; Kim S; Paik R; Sugino K; Kvitsani D; Fu Y; Lu J; Lin Y; Miyoshi G; Shima Y; Fishell G; Nelson SB; Huang ZJ. 2011. A resource of cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex. Neuron 71(6):995-1013. [PubMed: 21943598] [MGI Ref ID J:177261]
Additional References
Ccktm1.1(cre)Zjh relatedAllen Institute for Brain Science. 2009. Cre transgenes created at the Allen Insitute for Brain Science (AIBS) MGI Direct Data Submission :. [MGI Ref ID J:146821]
Garfield AS; Patterson C; Skora S; Gribble FM; Reimann F; Evans ML; Myers MG Jr; Heisler LK. 2012. Neurochemical characterization of body weight-regulating leptin receptor neurons in the nucleus of the solitary tract. Endocrinology 153(10):4600-7. [PubMed: 22869346] [MGI Ref ID J:191291]
Genetic Resource Sciences at The Jackson Laboratory. 2012. Expression/Specificity Patterns of Cre Alleles, 2012 MGI Direct Data Submission :. [MGI Ref ID J:184579]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together. Mating System Homozygote x Homozygote (Female x Male) 27-OCT-11 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Homozygous for Ccktm1.1(cre)Zjh
Price per Pair (US dollars $) Pair Genotype $464.00 Homozygous for Ccktm1.1(cre)Zjh x Homozygous for Ccktm1.1(cre)Zjh Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Homozygous for Ccktm1.1(cre)Zjh
Price per Pair (US dollars $) Pair Genotype $603.20 Homozygous for Ccktm1.1(cre)Zjh x Homozygous for Ccktm1.1(cre)Zjh Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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