Strain Name:

B6;129S6-Sun1tm1Mhan/J

Stock Number:

012715

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Availability:

Cryopreserved - Ready for recovery

Homozygous female and male Sun1 (Sad1 and UNC84 domain containing 1) targeted mutation mice are sterile due to massive apoptotic events that are induced in the gonads. Docking of chromosomal telomeres at the nuclear envelope is disrupted in both spermatocytes and oocytes. This strain may be useful in studies of meiosis, reproductive development, and fertility.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating Investigator Min Han,   Universityof Colorado, Boulder

Description
Homozygous female and male Sun1 (Sad1 and UNC84 domain containing 1) targeted mutation mice are sterile due to massive apoptotic events that are induced in the gonads. The testes of homozygous males are much smaller than those of wildtype and heterozygous animals. Spermatids and spermatozoa are completely absent and only abnormal spermatocyte-like cells accumulate in some tubules. Ovaries of homozygous females are much smaller than those of their littermates and ovarian follicles are completely absent. No oocytes are detected in day 5 neonatal mice. Docking of chromosomal telomeres at the nuclear envelope is disrupted in both spermatocytes and oocytes of homozygous animals. Efficient chromosomal homolog pairing and synapsis formation in meiosis are disrupted. These mice otherwise develop normally into adults and live for more than a year with no overt differences in physical appearance compared to wildtype littermates. This strain may be useful in studies of meiosis, reproductive development, and fertility.

Development
Exons 10-13 were replaced with a neomycin resistance cassette placed in reverse transcriptional orientation using 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Resultant chimeric mice were backcrossed one time to C57BL/6 by the donating laboratory.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Sun1tm1Mhan/Sun1tm1Mhan

        involves: 129S6/SvEvTac
  • reproductive system phenotype
  • abnormal female meiosis
    • oocytes fail to form normal nuclear attachment and clustering of telomers   (MGI Ref ID J:122602)
    • very rarely are pachytene-like structures observed   (MGI Ref ID J:122602)
  • abnormal ovary morphology
    • at E17.5, ovaries have increased apoptotic signal   (MGI Ref ID J:122602)
    • absent ovarian follicles   (MGI Ref ID J:122602)
    • small ovary   (MGI Ref ID J:122602)
  • abnormal seminiferous tubule morphology
    • apoptotic signal in the seminiferous tubules is increased   (MGI Ref ID J:122602)
  • abnormal spermatocyte morphology
    • only abnormal spermatocyte-like cells are present   (MGI Ref ID J:122602)
  • absent oocytes
    • no oocytes are detected at day 5   (MGI Ref ID J:122602)
  • arrest of male meiosis
    • spermatogenesis is arrested in meiotic prophase   (MGI Ref ID J:122602)
    • spermatocytes fail to form normal nuclear attachment and clustering of telomers   (MGI Ref ID J:122602)
    • no spermatocytes show normal homologous pairing and synapsis   (MGI Ref ID J:122602)
  • decreased male germ cell number
    • germ cells are largely depleted in seminiferous tubules   (MGI Ref ID J:122602)
    • spermatids and spermatozoa are completely absent   (MGI Ref ID J:122602)
  • female infertility   (MGI Ref ID J:122602)
  • male infertility   (MGI Ref ID J:122602)
  • small testis   (MGI Ref ID J:122602)
  • hearing/vestibular/ear phenotype
  • abnormal cochlear outer hair cell morphology
    • outer hair cells exhibit abnormal positioning of the nucleus compared with wild-type mice   (MGI Ref ID J:194486)
    • cochlear outer hair cell degeneration   (MGI Ref ID J:194486)
  • increased or absent threshold for auditory brainstem response
  • behavior/neurological phenotype
  • absent pinna reflex   (MGI Ref ID J:194486)
  • muscle phenotype
  • abnormal muscle morphology
    • mice exhibit a modest decrease in the average number of synaptic nuclei beneath the neuromuscular junctions compared with wild-type mice   (MGI Ref ID J:150047)
  • nervous system phenotype
  • abnormal cochlear outer hair cell morphology
    • outer hair cells exhibit abnormal positioning of the nucleus compared with wild-type mice   (MGI Ref ID J:194486)
    • cochlear outer hair cell degeneration   (MGI Ref ID J:194486)
  • endocrine/exocrine gland phenotype
  • abnormal ovary morphology
    • at E17.5, ovaries have increased apoptotic signal   (MGI Ref ID J:122602)
    • absent ovarian follicles   (MGI Ref ID J:122602)
    • small ovary   (MGI Ref ID J:122602)
  • abnormal seminiferous tubule morphology
    • apoptotic signal in the seminiferous tubules is increased   (MGI Ref ID J:122602)
  • small testis   (MGI Ref ID J:122602)

Sun1tm1Mhan/Sun1tm1Mhan

        B6.129S6-Sun1tm1Mhan
  • reproductive system phenotype
  • abnormal male meiosis
    • chromosome movement is impaired   (MGI Ref ID J:205574)
    • defect in telomere-nuclear envelope attachment   (MGI Ref ID J:205574)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Apoptosis Research

Reproductive Biology Research
Developmental Defects Affecting Gonads
      germ cell deficient
Fertility Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Sun1tm1Mhan
Allele Name targeted mutation 1, Min Han
Allele Type Targeted (Null/Knockout)
Common Name(s) Sun1(delta ex10-13); Sun1-;
Mutation Made By Min Han,   Universityof Colorado, Boulder
Strain of Origin129S6/SvEvTac
Gene Symbol and Name Sun1, Sad1 and UNC84 domain containing 1
Chromosome 5
Gene Common Name(s) 4632417G13Rik; 5730434D03Rik; RIKEN cDNA 4632417G13 gene; RIKEN cDNA 5730434D03 gene; UNC84A; Unc84a; mKIAA0810; unc-84 homolog A (C. elegans);
Molecular Note Exons encoding all three putative transmembrane domains of SUN1 (exons 10-13) were replaced by a neomycin resistance gene. RT-PCR confirmed absence of normal mRNA transcripts in mutant testis and muscle. [MGI Ref ID J:122602]

Genotyping

Genotyping Information

Genotyping Protocols

Sun1tm1Mhan, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Ding X; Xu R; Yu J; Xu T; Zhuang Y; Han M. 2007. SUN1 is required for telomere attachment to nuclear envelope and gametogenesis in mice. Dev Cell 12(6):863-72. [PubMed: 17543860]  [MGI Ref ID J:122602]

Additional References

Sun1tm1Mhan related

Horn HF; Brownstein Z; Lenz DR; Shivatzki S; Dror AA; Dagan-Rosenfeld O; Friedman LM; Roux KJ; Kozlov S; Jeang KT; Frydman M; Burke B; Stewart CL; Avraham KB. 2013. The LINC complex is essential for hearing. J Clin Invest :. [PubMed: 23348741]  [MGI Ref ID J:194486]

Lei K; Zhang X; Ding X; Guo X; Chen M; Zhu B; Xu T; Zhuang Y; Xu R; Han M. 2009. SUN1 and SUN2 play critical but partially redundant roles in anchoring nuclei in skeletal muscle cells in mice. Proc Natl Acad Sci U S A 106(25):10207-12. [PubMed: 19509342]  [MGI Ref ID J:150047]

Lei K; Zhu X; Xu R; Shao C; Xu T; Zhuang Y; Han M. 2012. Inner nuclear envelope proteins SUN1 and SUN2 play a prominent role in the DNA damage response. Curr Biol 22(17):1609-15. [PubMed: 22863315]  [MGI Ref ID J:199760]

Razafsky D; Blecher N; Markov A; Stewart-Hutchinson PJ; Hodzic D. 2012. LINC complexes mediate the positioning of cone photoreceptor nuclei in mouse retina. PLoS One 7(10):e47180. [PubMed: 23071752]  [MGI Ref ID J:192230]

Shibuya H; Ishiguro K; Watanabe Y. 2014. The TRF1-binding protein TERB1 promotes chromosome movement and telomere rigidity in meiosis. Nat Cell Biol 16(2):145-56. [PubMed: 24413433]  [MGI Ref ID J:205574]

Yu J; Lei K; Zhou M; Craft CM; Xu G; Xu T; Zhuang Y; Xu R; Han M. 2011. KASH protein Syne-2/Nesprin-2 and SUN proteins SUN1/2 mediate nuclear migration during mammalian retinal development. Hum Mol Genet 20(6):1061-73. [PubMed: 21177258]  [MGI Ref ID J:168852]

Zhang X; Lei K; Yuan X; Wu X; Zhuang Y; Xu T; Xu R; Han M. 2009. SUN1/2 and Syne/Nesprin-1/2 complexes connect centrosome to the nucleus during neurogenesis and neuronal migration in mice. Neuron 64(2):173-87. [PubMed: 19874786]  [MGI Ref ID J:155113]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, heterozygotes may be bred. Homozygous males and females are sterile.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3300.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4290.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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