Strain Name:

B6;129-Fzd8tm1Nat/J

Stock Number:

012826

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Availability:

Cryopreserved - Ready for recovery

These Fzd8 (frizzled homolog 8 (Drosophila)) homozygous targeted mice show no obvious phenotype. When examined in combination with a homozygous loss of Fzd4 (see Stock No. 012823), however there is a defect in kidney development. LacZ expression replaces that of the targeted gene. This strain may be useful in studying the function of this receptor.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating Investigator Jeremy Nathans,   Johns Hopkins University

Description
These Fzd8 (frizzled homolog 8 (Drosophila)) homozygous targeted mice show no obvious phenotype. When examined in combination with a homozygous loss of Fzd4 (see Stock No. 012823), however there is a defect in kidney development. LacZ expression replaces that of the targeted gene. This strain may be useful in studying the function of this receptor.

Development
The open reading frame of the targeted gene was replaced by beta-galactosidase (LacZ) via homologous recombination in a 129-derived embryonic stem (ES) cell line. A PGK-neo selectable marker used for ES cell selection is present downstream of the LacZ sequences. This strain was maintained on a mixed C57BL/6 and 129 background by the donating laboratory.

Related Strains

Strains carrying other alleles of Fzd8
005804   B6.129P2-Fzd8tm1Dgen/J
View Strains carrying other alleles of Fzd8     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Fzd8tm1Nat/Fzd8tm1Nat

        involves: 129S1/Sv * 129X1/SvJ
  • normal phenotype
  • no abnormal phenotype detected
    • mice are viable, normal sized, and fertile   (MGI Ref ID J:170839)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Internal/Organ Defects
      kidney

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Fzd8tm1Nat
Allele Name targeted mutation 1, Jeremy Nathans
Allele Type Targeted (Reporter)
Common Name(s) Fz8lacZ;
Mutation Made By Jeremy Nathans,   Johns Hopkins University
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Fzd8, frizzled homolog 8 (Drosophila)
Chromosome 18
Gene Common Name(s) FZ-8; Fz8; hFZ8; mFZ8;
Molecular Note The open reading frame of the targeted gene was replaced by beta-galactosidase (LacZ) via homologous recombination in a 129-derived embryonic stem (ES) cell line. A PGK-neo selectable marker used for ES cell selection is present downstream of the LacZ sequences. [MGI Ref ID J:159436] [MGI Ref ID J:170839]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Nathans J. 2010. DDS for Fzd8 MGI Direct Data Submission :.  [MGI Ref ID J:159436]

Additional References

Fzd8tm1Nat related

Liu C; Bakeri H; Li T; Swaroop A. 2012. Regulation of retinal progenitor expansion by Frizzled receptors: implications for microphthalmia and retinal coloboma. Hum Mol Genet 21(8):1848-60. [PubMed: 22228100]  [MGI Ref ID J:181885]

Ye X; Wang Y; Rattner A; Nathans J. 2011. Genetic mosaic analysis reveals a major role for frizzled 4 and frizzled 8 in controlling ureteric growth in the developing kidney. Development 138(6):1161-72. [PubMed: 21343368]  [MGI Ref ID J:170839]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, homozygotes or heterozygotes may be bred.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2085.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2710.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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