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| These CD4-IL-17RB transgenic mice may be useful in studies of T cell differentiation and allergic immune response. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Chen Dong, MD Anderson Cancer Center Description
These transgenic mice express the mouse interleukin 17 receptor B under the direction of the mouse CD4 antigen promoter/enhancer. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. The transgene is expressed in all T cells. When treated with IL25, T cells from transgenic mice have reduced numbers of IFN-gamma producing cells, reduced IL4 and IFN-gamma levels and increased IL5 and IL13 levels when compared to wildtype. Transgenic mice challenged with intranasal allergens exhibit higher levels of inflammatory cell infiltration than wildtype controls.Development
A transgenic construct containing the mouse interleukin 17 receptor B, Il17rb, gene under the control of the mouse CD4 antigen, Cd4, promoter/enhancer with the first intron without the silencer element, was injected into fertilized C57BL/6J mouse eggs. Founder line 5 was subsequently established. Upon arrival at The Jackson Laboratory the mice were crossed to C57BL/6J at least once to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Cd4
002447 B10.129S2(B6)-Cd4tm1Litt/J 010514 B10.Cg-H2g Tg(Cd4-Klra1)6295Dl/J 002663 B6.129S2-Cd4tm1Mak/J 002269 B6.129S6-Cd4tm1Knw/J 002664 B6;129S-Cd4tm1Mak Cd8atm1Mak/J 002268 B6;129S6-Cd4tm1Knw/J 014639 C57BL/6-Tg(Cd4-TcraDN32D3)1Aben/J 014644 C57BL/6-Tg(Cd4-Zbtb16)1797Aben/J 006483 CByJ.129S2(B6)-Cd4tm1Mak/J 003090 NOD.129S6(B6)-Cd4tm1Knw/DvsJ 013234 NOD.Cg-Tg(Cd4-cre)1Cwi/2AchJ 005328 NOD/ShiLt-Tg(Cd4-DsRed)4Lt/J 017336 STOCK Tg(Cd4-cre)1Cwi/BfluJ View Strains carrying other alleles of Cd4 (13 strains)
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Immunology, Inflammation and Autoimmunity Research
Immunodeficiency
T cell deficiency
Research Tools
Immunology and Inflammation Research
T cell deficiency
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Cd4-Il17rb)5Cdon | ||
|---|---|---|---|
| Allele Name | transgene insertion 5, Chen Dong | ||
| Allele Type | Transgenic (random, expressed) | ||
| Mutation Made By | Chen Dong, MD Anderson Cancer Center | ||
| Strain of Origin | C57BL/6 | ||
| Expressed Gene | Il17rb, interleukin 17 receptor B, mouse, laboratory | ||
| Promoter | Cd4, CD4 antigen, mouse, laboratory | ||
| Molecular Note | A full length mouse Il17b cDNA was inserted behind the mouse Cd4 promoter/enhancer and the first intron without the silencer element. Three (1, 3 and 5) lines with the highest mRNA expression were studied. [MGI Ref ID J:158594] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Cd4-Il17rb)5Cdon, Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Angkasekwinai P; Chang SH; Thapa M; Watarai H; Dong C. 2010. Regulation of IL-9 expression by IL-25 signaling. Nat Immunol 11(3):250-6. [PubMed: 20154671] [MGI Ref ID J:158594]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgene carrier mice may be bred with wildtype (noncarrier) mice from the colony or with C57BL/6J inbred mice (Stock No. 000664). The Donating Investigator has not attempted to make the strain homozygous.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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