Strain Name:

B6;129S-Stat1tm1Mam/Mmjax

Availability:

Cryopreserved - Ready for recovery     Available at the JAX MMRRC

Please refer to the Mutant Mouse Regional Resource Center (MMRRC) for information about B6;129S-Stat1tm1Mam/Mmjax MMRRC Stock Number 032054.
These Stat1fl (signal transducer and activator of transcription 1) mutant mice may be useful in generating conditional mutations to study autonomous roles of STAT1 in ERBB2/neu induced mammary tumorigenesis.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
Generation?pN1
Generation Definitions
 
Donating InvestigatorDr. Lothar Hennighausen,   National Institutes of Health

Description
Mice homozygous for the targeted mutation are viable and fertile and exhibit no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific conditional mutants of the floxed gene. This mutant mouse strain may be useful in generating conditional mutations to study autonomous roles of STAT1 in ERBB2/neu induced mammary tumorigenesis.

Heterozygote: Not evaluated

Development
A targeting vector was designed to place a loxP flanked neomycin resistance gene upstream of the first untranslated exon followed by a loxP site downstream of the first translated exon. The construct was electroporated into 129S/SvEv derived embryonic stem (ES) cells. Transient Cre expression in targeted cells excised the neo cassette leaving loxP sites flanking the first two untranslated exons and the first translated exon. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice and maintained as sibling matings. Upon arrival, mice were bred to C57BL/6 for at least 1 generation to establish the colony.

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Stat1
012606   B6.129S(Cg)-Stat1tm1Dlv/J
View Strains carrying other alleles of Stat1     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Atypical Mycobacteriosis, Familial   (STAT1)
Candidiasis, Familial, 7; CANDF7   (STAT1)
Mycobacterial and Viral Infections, Susceptibility to, Autosomal Recessive   (STAT1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Stat1tm1Mam/Stat1tm1Mam Tg(MMTV-Erbb2*,-cre)1Mul/0

        involves: 129S/SvEv * FVB   (conditional)
  • tumorigenesis
  • decreased tumor latency
    • median time to tumor onset is 49 weeks compared with 62 weeks for Tg(MMTV-Erbb2*,-cre)1Mul mice   (MGI Ref ID J:169262)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cancer Research
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Stat1tm1Mam
Allele Name targeted mutation 1, Lothar Hennighausen
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Stat1 fl/fl;
Mutation Made ByDr. Lothar Hennighausen,   National Institutes of Health
Strain of Origin129S/SvEv
Gene Symbol and Name Stat1, signal transducer and activator of transcription 1
Chromosome 1
Gene Common Name(s) 2010005J02Rik; AA408197; CANDF7; DD6G4-4; ISGF-3; RIKEN cDNA 2010005J02 gene; STAT91; expressed sequence AA408197;
Molecular Note A targeting vector was designed to place a loxP flanked neomycin resistance gene upstream of the first untranslated exon followed by a loxP site downstream of the first translated exon. The neo cassette was removed by germ-line cre-mediated resombination leaving loxP sites flanking the first two untranslated exons and the first translated exon. [MGI Ref ID J:163376]

Genotyping

Genotyping Information

Genotyping Protocols

Stat1tm1Mam, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Klover P; Hennighausen L. 2010. Generation of a Stat1 conditional allele MGI Direct Data Submission :.  [MGI Ref ID J:163376]

Additional References

Stat1tm1Mam related

Klover PJ; Muller WJ; Robinson GW; Pfeiffer RM; Yamaji D; Hennighausen L. 2010. Loss of STAT1 from mouse mammary epithelium results in an increased neu-induced tumor burden. Neoplasia 12(11):899-905. [PubMed: 21076615]  [MGI Ref ID J:169262]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhile maintaining a live colony, these mice are bred as homozygotes.

Pricing and Purchasing

Supply Notes


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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