|Please refer to the Mutant Mouse Regional Resource Center (MMRRC) for information about B6;129S-Stat1tm1Mam/Mmjax MMRRC Stock Number 032054.|
|These Stat1fl (signal transducer and activator of transcription 1) mutant mice may be useful in generating conditional mutations to study autonomous roles of STAT1 in ERBB2/neu induced mammary tumorigenesis.|
Type Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?pN1
Donating Investigator Dr. Lothar Hennighausen, National Institutes of Health
Mice homozygous for the targeted mutation are viable and fertile and exhibit no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific conditional mutants of the floxed gene. This mutant mouse strain may be useful in generating conditional mutations to study autonomous roles of STAT1 in ERBB2/neu induced mammary tumorigenesis.
Heterozygote: Not evaluated
A targeting vector was designed to place a loxP flanked neomycin resistance gene upstream of the first untranslated exon followed by a loxP site downstream of the first translated exon. The construct was electroporated into 129S/SvEv derived embryonic stem (ES) cells. Transient Cre expression in targeted cells excised the neo cassette leaving loxP sites flanking the first two untranslated exons and the first translated exon. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice and maintained as sibling matings. Upon arrival, mice were bred to C57BL/6 for at least 1 generation to establish the colony.
|Considerations for Choosing Controls|
Strains carrying other alleles of Stat1
012606 B6.129S(Cg)-Stat1tm1Dlv/JView Strains carrying other alleles of Stat1 (1 strain)
View Related Disease (OMIM) TermsRelated Disease (OMIM) Terms provided by MGIView Mammalian Phenotype TermsMammalian Phenotype Terms provided by MGIassigned by genotype
Stat1tm1Mam/Stat1tm1Mam Tg(MMTV-Erbb2*,-cre)1Mul/0involves: 129S/SvEv * FVB (conditional)
- decreased tumor latency
- median time to tumor onset is 49 weeks compared with 62 weeks for Tg(MMTV-Erbb2*,-cre)1Mul mice (MGI Ref ID J:169262)View Research ApplicationsResearch ApplicationsThis mouse can be used to support research in many areas including:
|Allele Name||targeted mutation 1, Lothar Hennighausen|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Common Name(s)||Stat1 fl/fl;|
|Mutation Made By||Dr. Lothar Hennighausen, National Institutes of Health|
|Strain of Origin||129S/SvEv|
|Gene Symbol and Name||Stat1, signal transducer and activator of transcription 1|
|Gene Common Name(s)||2010005J02Rik; AA408197; CANDF7; DD6G4-4; IMD31A; IMD31B; IMD31C; ISGF-3; RIKEN cDNA 2010005J02 gene; STAT91; expressed sequence AA408197;|
|Molecular Note||A targeting vector was designed to place a loxP flanked neomycin resistance gene upstream of the first untranslated exon followed by a loxP site downstream of the first translated exon. The neo cassette was removed by germ-line cre-mediated resombination leaving loxP sites flanking the first two untranslated exons and the first translated exon. [MGI Ref ID J:163376]|
Klover P; Hennighausen L. 2010. Generation of a Stat1 conditional allele MGI Direct Data Submission :. [MGI Ref ID J:163376]
Capitini CM; Nasholm NM; Chien CD; Larabee SM; Qin H; Song YK; Klover PJ; Hennighausen L; Khan J; Fry TJ. 2014. Absence of STAT1 in donor-derived plasmacytoid dendritic cells results in increased STAT3 and attenuates murine GVHD. Blood 124(12):1976-86. [PubMed: 25079358] [MGI Ref ID J:214606]
Klover PJ; Muller WJ; Robinson GW; Pfeiffer RM; Yamaji D; Hennighausen L. 2010. Loss of STAT1 from mouse mammary epithelium results in an increased neu-induced tumor burden. Neoplasia 12(11):899-905. [PubMed: 21076615] [MGI Ref ID J:169262]
Animal Health ReportsProduction of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Breeding & Husbandry While maintaining a live colony, these mice are bred as homozygotes.
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