Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.Cg-Tg(S100a4-TK)M31Egn/YunkJ    (Changed: 17-AUG-12 ) B6.Cg-Tg(S100a4-Tk)M31Egn/YunkJ    (Changed: 05-SEP-11 ) B6.Cg-Tg(S100a4-TK*)M31Egn/YunkJ    (Changed: 11-JUN-10 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N14+N4pN1 Generation Definitions   Donating Investigator Eric G. Neilson,   Vanderbilt University Donating Investigator Dr. Kyuson Yun,   JAX

Description
These transgenic mice express a truncated herpesvirus thymidine kinase (TK) gene under the control of the S100a4, S100 calcium binding protein A4, (also called FSP1, fibroblast-specific protein-1), promoter. The truncated TK minigene lacks the cryptic promoter between the first and second ATG of the coding sequence which is active in testes and confers male sterility. The transgene was found to be expressed in the interstitium of the kidney and transgene mRNA is expressed at higher levels in fibroblasts than in renal tubular epithelium. Dividing (proliferating) fibroblasts are selectively ablated in transgenic mice when treated with gancyclovir. Cultured primary fibroblasts from transgenic mice exhibit significant depletion 5 days after 10 mM gancyclovir treatment. Cultured renal tubular epithelium cells are not gancyclovir sensitive. Transgenic mice exhibit reduced fibrosis in experimentally induced renal obstruction. Founder line M31 contains several copies of the transgene. Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities.

Development
A transgenic construct containing a truncated herpesvirus thymidine kinase (TK) gene under the control of the mouse S100a4, S100 calcium binding protein A4, (also called FSP1, fibroblast-specific protein-1), promoter was injected into (B6 X SJL)F1 blastocysts. Founder line M31 animals were bred to wildtype BALB/c mice. The mice were backcrossed onto the C57BL/6 background for 14 generations, and then to the C57BL/6J background for an additional 4 generations. Upon arrival at The Jackson Laboratory Repository, the mice were crossed to C57BL/6J at least once to establish the colony.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of HSV-TK

017523	129S6.Cg-Tg(Gfap-TK)7.1Mvs/RhnJ
005698	B6.Cg-Tg(Gfap-TK)7.1Mvs/J
005999	B6.Cg-Tg(SBE/TK-luc)7Twc/J
012943	C57BL/6-Tg(Ins2-luc/EGFP/TK)300Kauf/J
013116	NOD.B6-Tg(Ins2-luc/EGFP/TK)300Kauf/J

View Strains carrying other alleles of HSV-TK     (5 strains)

Strains carrying other alleles of S100a4

012904	B6.129S6-S100a4tm1Egn/YunkJ
012893	B6.Cg-Tg(S100a4-EGFP)M1Egn/YunkJ
012641	BALB/c-Tg(S100a4-cre)1Egn/YunkJ

View Strains carrying other alleles of S100a4     (3 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Tg(S100a4-TK)M31Egn/0

        involves: BALB/c * C57BL/6 * SJL

• cellular phenotype
• decreased sensitivity to induced cell death
  • cultured renal tubular epithelium cells do not exhibit sensitivity to gancyclovir unlike similarly treated 3T3 fibroblasts   (MGI Ref ID J:160213)
• increased sensitivity to induced cell death
  • mice subjected to surgical ureteral obstruction and treated with gancyclovir exhibit fewer S100a4+ cells in obstructed kidneys compared with similarly treated wild-type mice or Tg(S100a4-Tk)M31Egn mice subjected to surgical ureteral obstruction and treated with vehicle alone   (MGI Ref ID J:160213)
  • cultured fibroblast cells exhibit increased sensitivity to gancyclovir unlike similarly treated wild-type fibroblasts   (MGI Ref ID J:160213)
• homeostasis/metabolism phenotype
• abnormal physiological response to xenobiotic
  • mice subjected to surgical ureteral obstruction and treated with gancyclovir exhibit fewer S100a4+ cells and decreased fibrosis in obstructed kidneys compared with similarly treated wild-type mice or Tg(S100a4-Tk)M31Egn mice subjected to surgical ureteral obstruction and treated with vehicle alone   (MGI Ref ID J:160213)
• abnormal response to injury
  • mice subjected to surgical ureteral obstruction and treated with gancyclovir exhibit fewer S100a4+ cells and decreased fibrosis in obstructed kidneys compared with similarly treated wild-type mice or Tg(S100a4-Tk)M31Egn mice subjected to surgical ureteral obstruction and treated with vehicle alone   (MGI Ref ID J:160213)
• renal/urinary system phenotype
• abnormal kidney morphology
  • mice subjected to surgical ureteral obstruction and treated with gancyclovir exhibit fewer S100a4+ cells and decreased fibrosis in obstructed kidneys compared with similarly treated wild-type mice or Tg(S100a4-Tk)M31Egn mice subjected to surgical ureteral obstruction and treated with vehicle alone   (MGI Ref ID J:160213)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Internal/Organ Research
Wound Healing

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Tg(S100a4-TK)M31Egn
Allele Name		transgene insertion M31, Eric G Neilson
Allele Type		Transgenic (random, expressed)
Common Name(s)		FSP1.DTK; FSP1.deltaTK;
Strain of Origin		(C57BL/6 x SJL)F1
Expressed Gene		HSV-TK, herpes simplex virus thymidine kinase,
Promoter		S100a4, S100 calcium binding protein A4, mouse, laboratory
Molecular Note		The transgenic construct contained a truncated herpesvirus thymidine kinase (TK) gene under the control of the mouse S100a4 promoter. Three lines were created with line M31 selected as the representative line. [MGI Ref ID J:160213]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(S100a4-TK*), Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Iwano M; Fischer A; Okada H; Plieth D; Xue C; Danoff TM; Neilson EG. 2001. Conditional abatement of tissue fibrosis using nucleoside analogs to selectively corrupt DNA replication in transgenic fibroblasts. Mol Ther 3(2):149-59. [PubMed: 11237671]  [MGI Ref ID J:160213]

Additional References

Tg(S100a4-TK)M31Egn related

O'Connell JT; Sugimoto H; Cooke VG; Macdonald BA; Mehta AI; Lebleu VS; Dewar R; Rocha RM; Brentani RR; Resnick MB; Neilson EG; Zeisberg M; Kalluri R. 2011. VEGF-A and Tenascin-C produced by S100A4+ stromal cells are important for metastatic colonization. Proc Natl Acad Sci U S A 108(38):16002-7. [PubMed: 21911392]  [MGI Ref ID J:176884]

Zhang J; Chen L; Xiao M; Wang C; Qin Z. 2011. FSP1+ fibroblasts promote skin carcinogenesis by maintaining MCP-1-mediated macrophage infiltration and chronic inflammation. Am J Pathol 178(1):382-90. [PubMed: 21224075]  [MGI Ref ID J:168079]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, transgene carrier mice may be bred with wildtype (noncarrier) mice from the colony or with C57BL/6J inbred mice (Stock No. 000664). The Donating Investigator has not attempted to make the strain homozygous.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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