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| The nestin-CreER transgene contains the rat nestin enhancer/hsp68 minimal promoter directing expression of a tamoxifen-inducible Cre recombinase (Cre-ERT1). When these nestin-CreER transgenic mice are bred with mice containing a loxP-flanked sequence, tamoxifen-inducible Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring. These mice may be useful for studying embryonic and adult neurogenesis. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 19-OCT-10 Species laboratory mouse Generation F?+N2F1 (29-JUN-11)
Generation DefinitionsDonating Investigator Chia-Yi Kuan, Cincinnati Children's Hospital Med Ctr Description
Mice homozygous for the nestin-CreER transgene are viable and fertile, with the rat nestin enhancer/hsp68 minimal promoter directing expression of a tamoxifen-inducible Cre recombinase (Cre-ERT1). This Cre-ERT1 fusion protein is estrogen insensitive, and is only active when it binds the estrogen analog 4-hydroxytamoxifen (OHT or tamoxifen). Following tamoxifen administration, Cre recombinase activity is reported in adult neural progenitors in the subventricular zone (SVZ), perinatal SVZ glioblasts, embryonic neural progenitors, and postnatally transformed radial glial cells. When these nestin-CreER transgenic mice are bred with mice containing a loxP-flanked sequence, tamoxifen-inducible Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring. These mice may be useful for studying embryonic and adult neurogenesis.The Cre-ERT1 fusion protein consists of Cre recombinase fused to a G525R mutant form of the mouse estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered.
Development
This nestin-CreER transgene was designed to place a CreERT1 fusion gene (Cre-ERT1; Cre recombinase fused to a G525R mutant form of the mouse estrogen receptor ligand binding domain) under the control of the rat nestin second intron enhancer fused to an hsp68 (Hspa1b) minimal promoter. The transgene was microinjected into fertilized C57BL/6 oocytes, and founder mice were bred to C57BL/6 mice. The donating investigator reported that these resulting transgenic mice were backcrossed to C57BL/6 mice (see SNP note below) to maintain the nestin-CreER colony. Upon arrival at The Jackson Laboratory Repository, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Nes
003771 B6.Cg-Tg(Nes-cre)1Kln/J 016261 C57BL/6-Tg(Nes-cre/ERT2)KEisc/J 004127 FVB-Tg(Nes-rtTA)306Rvs/J View Strains carrying other alleles of Nes (3 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
Tg(Nes-cre/Esr1*)1Kuan/Tg(Nes-cre/Esr1*)1Kuan
involves: C57BL/6
- normal phenotype
- no abnormal phenotype detected
- mice are viable and fertile (MGI Ref ID J:156372)
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression: Inducible
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: astrocytes, neurons
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Nes-cre/Esr1*)1Kuan | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Chia-Yi Kuan | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Tg(Nes-cre/ER)1Kuan; nestin-CreER; | ||
| Mutation Made By | Chia-Yi Kuan, Cincinnati Children's Hospital Med Ctr | ||
| Strain of Origin | C57BL/6 | ||
| Site of Expression | Following tamoxifen administration, Cre recombinase activity is reported in adult neural progenitors in the subventricular zone (SVZ), perinatal SVZ glioblasts, embryonic neural progenitors, and postnatally transformed radial glial cells. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Nes, nestin, rat | ||
| Driver Note | Nes | ||
| Inducible Note | induced by tamoxifen | ||
| Molecular Note | The transgene construct contains the rat nestin second intron enhancer fused to an hsp68 (Hspa1b) minimal promoter driving a 1.6 kb cre sequence fused to the tamoxifen-inducible mutatant estrogen receptor (Esr1*). The linearized construct was microinjected into pronuclei of fertilized C57BL/6 eggs. Three lines of nestin-cre/Esr1* mice were generated. [MGI Ref ID J:156372] | ||
| Gene Symbol and Name | Tg(Nes-cre/Esr1*)1Kuan, transgene insertion 1, Chia-Yi Kuan | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Tg(Nes-cre/ERT)1Kuan; Tg(Nes-cre/ERT2)1Kuan; nestin-CreER; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Burns KA; Ayoub AE; Breunig JJ; Adhami F; Weng WL; Colbert MC; Rakic P; Kuan CY. 2007. Nestin-CreER mice reveal DNA synthesis by nonapoptotic neurons following cerebral ischemia hypoxia. Cereb Cortex 17(11):2585-92. [PubMed: 17259645] [MGI Ref ID J:156372]
Additional References
Tg(Nes-cre/Esr1*)1Kuan relatedBurns KA; Murphy B; Danzer SC; Kuan CY. 2009. Developmental and post-injury cortical gliogenesis: a genetic fate-mapping study with Nestin-CreER mice. Glia 57(10):1115-29. [PubMed: 19115384] [MGI Ref ID J:156208]
Ma S; Kwon HJ; Huang Z. 2012. Ric-8a, a guanine nucleotide exchange factor for heterotrimeric G proteins, regulates bergmann glia-basement membrane adhesion during cerebellar foliation. J Neurosci 32(43):14979-93. [PubMed: 23100420] [MGI Ref ID J:191222]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgene carrier mice may be bred with wildtype (noncarrier) mice from the colony or with C57BL/6J inbred mice (Stock No. 000664). Mating System Noncarrier x Hemizygote (Female x Male) 19-OCT-10 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(Nes-cre/Esr1*)1Kuan
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(Nes-cre/Esr1*)1Kuan x Noncarrier $296.00 Noncarrier x Hemizygous for Tg(Nes-cre/Esr1*)1Kuan Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(Nes-cre/Esr1*)1Kuan
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(Nes-cre/Esr1*)1Kuan x Noncarrier $384.80 Noncarrier x Hemizygous for Tg(Nes-cre/Esr1*)1Kuan Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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