Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse   Donating Investigator Dong-Ming Su,   Univ. of TX Health Science Center Tyler

Description
These mice possess loxP sites on either side of exons 5 and 6 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. RT-PCR analysis of thymic cells isolated from homozygotes reveals the gene product (mRNA) levels are the same as wildtype control. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 5 and 6 deleted in the cre-expressing tissue(s).

When bred to a strain with inducible widespread Cre recombinase expression (see Stock No. 004682 for example), this mutant mouse strain may be useful in studies of thymic epithelial differentiation and T cell development.

For example, when bred to mice carrying Tg(KRT5-cre/ERT2)2Ipc (see Stock No. 018394 tamoxifen-induced Cre-mediated recombination resultsin mice that develop significant thymic atrophy due to increased apoptosis associated with p53 activation.

Development
A FRT site flanked PGK-Neo cassette containing a loxP site, was inserted downstream of exon 6 of the targeted gene, and another loxP site was inserted upstream of exon 5. This construct was electroporated into unspecified 129 derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to mice expressing FLP recombinase under the control of the human beta actin promoter, B6;SJL-Tg(ACTFLPe)9205Dym/J (Stock No. 003800). Mice that retained the loxP site flanked exons 5 and 6 were then backcrossed to C57BL/6 for 5 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Foxn1

000820	AKR/J-Foxn1nu-str/J
018448	B6(Cg)-Foxn1tm3(cre)Nrm/J
016195	B6(SJL)-Foxn1nu-2J/GrsrJ
000521	B6.AK-Foxn1nu-str/J
000819	B6.Cg-Foxn1nu/J
000711	CByJ.Cg-Foxn1nu/J
007850	J:NU
002019	NU/J
003118	STOCK Ces1ce Foxn1nu/J

View Strains carrying other alleles of Foxn1     (9 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. T-Cell Immunodeficiency, Congenital Alopecia, and Nail Dystrophy   (FOXN1)

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Foxn1^tm1.1Dmsu/Foxn1^tm1.1Dmsu

        involves: 129/Sv * C57BL/6 * SJL

• normal phenotype
• no abnormal phenotype detected
  • mice are normal   (MGI Ref ID J:159773)

Foxn1^tm1.1Dmsu/Foxn1^tm1.1Dmsu Tg(KRT5-cre/ERT2)2Ipc/0

        involves: 129/Sv * C57BL/6 * SJL   (conditional)

• immune system phenotype
• abnormal thymus corticomedullary boundary morphology
  • following tamoxifen treatment, the medullary region architecture is altered and the corticomedullary junction becomes indistinct compared to in wild-type mice   (MGI Ref ID J:159773)
• abnormal thymus medulla morphology
  • following tamoxifen treatment, the medullary region architecture is altered and the corticomedullary junction becomes indistinct compared to in wild-type mice   (MGI Ref ID J:159773)
• decreased thymocyte number
  • following tamoxifen treatment   (MGI Ref ID J:159773)
• small thymus
  • following tamoxifen treatment   (MGI Ref ID J:159773)
• • thymus atrophy
    • following tamoxifen treatment   (MGI Ref ID J:159773)
• hematopoietic system phenotype
• abnormal thymus corticomedullary boundary morphology
  • following tamoxifen treatment, the medullary region architecture is altered and the corticomedullary junction becomes indistinct compared to in wild-type mice   (MGI Ref ID J:159773)
• abnormal thymus medulla morphology
  • following tamoxifen treatment, the medullary region architecture is altered and the corticomedullary junction becomes indistinct compared to in wild-type mice   (MGI Ref ID J:159773)
• decreased thymocyte number
  • following tamoxifen treatment   (MGI Ref ID J:159773)
• small thymus
  • following tamoxifen treatment   (MGI Ref ID J:159773)
• • thymus atrophy
    • following tamoxifen treatment   (MGI Ref ID J:159773)

The following phenotype relates to a compound genotype created using this strain.
Contact JAX^® Services jaxservices@jax.org for customized breeding options.

Foxn1^tm1.1Dmsu/Foxn1^tm1.1Dmsu Tg(CAG-cre/Esr1*)5Amc/0

        involves: 129/Sv * C57BL/6 * CBA * SJL   (conditional)

• immune system phenotype
• abnormal thymus morphology
  • following tamoxifen treatment, mice exhibit a decrease in thymic stromal cells compared to in wild-type mice   (MGI Ref ID J:159773)
• • decreased thymocyte number
    • following tamoxifen treatment   (MGI Ref ID J:159773)
  • small thymus
    • following tamoxifen treatment   (MGI Ref ID J:159773)
  • • thymus atrophy
      • following tamoxifen treatment   (MGI Ref ID J:159773)
• decreased double-positive T cell number
  • following tamoxifen treatment   (MGI Ref ID J:159773)
• hematopoietic system phenotype
• abnormal thymus morphology
  • following tamoxifen treatment, mice exhibit a decrease in thymic stromal cells compared to in wild-type mice   (MGI Ref ID J:159773)
• • decreased thymocyte number
    • following tamoxifen treatment   (MGI Ref ID J:159773)
  • small thymus
    • following tamoxifen treatment   (MGI Ref ID J:159773)
  • • thymus atrophy
      • following tamoxifen treatment   (MGI Ref ID J:159773)
• decreased double-positive T cell number
  • following tamoxifen treatment   (MGI Ref ID J:159773)
• cellular phenotype
• decreased double-positive T cell number
  • following tamoxifen treatment   (MGI Ref ID J:159773)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Foxn1tm1.1Dmsu
Allele Name		targeted mutation 1.1, Dong-Ming Su
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		FoxN1fx;
Mutation Made By		Dong-Ming Su,   Univ. of TX Health Science Center Tyler
Strain of Origin		129
Gene Symbol and Name		Foxn1, forkhead box N1
Chromosome		11
Gene Common Name(s)		D11Bhm185e; DNA segment, Chr 11, Boehm 185, expressed; FKHL20; HNF-3/forkhead homolog 11; Hfh11; RONU; Rnu; WHN; nu; nude;
Molecular Note		Flp mediated recombination removed the neo cassette leaving exons 5 and 6 floxed. [MGI Ref ID J:159773]

Genotyping

Genotyping Information

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Cheng L; Guo J; Sun L; Fu J; Barnes PF; Metzger D; Chambon P; Oshima RG; Amagai T; Su DM. 2010. Postnatal tissue-specific disruption of transcription factor FoxN1 triggers acute thymic atrophy. J Biol Chem 285(8):5836-47. [PubMed: 19955175]  [MGI Ref ID J:159773]

Additional References

Foxn1^tm1.1Dmsu related

Guo J; Rahman M; Cheng L; Zhang S; Tvinnereim A; Su DM. 2011. Morphogenesis and maintenance of the 3D thymic medulla and prevention of nude skin phenotype require FoxN1 in pre- and post-natal K14 epithelium. J Mol Med (Berl) 89(3):263-77. [PubMed: 21109991]  [MGI Ref ID J:187504]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering Information
JAX^® Mice
Surgical and Preconditioning Services
JAX^® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.