Strain Name:

B6.129(SJL)-Foxn1tm1.1Dmsu/J

Stock Number:

012941

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These Foxn1fx/fx mutant mice may be useful in generating conditional mutations for studying thymic development, hair follicle and nail development.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating Investigator Dong-Ming Su,   Univ. of TX Health Science Center Tyler

Description
These mice possess loxP sites on either side of exons 5 and 6 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. RT-PCR analysis of thymic cells isolated from homozygotes reveals the gene product (mRNA) levels are the same as wildtype control. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 5 and 6 deleted in the cre-expressing tissue(s).

When bred to a strain with inducible widespread Cre recombinase expression (see Stock No. 004682 for example), this mutant mouse strain may be useful in studies of thymic epithelial differentiation and T cell development.

For example, when bred to mice carrying Tg(KRT5-cre/ERT2)2Ipc (see Stock No. 018394 tamoxifen-induced Cre-mediated recombination resultsin mice that develop significant thymic atrophy due to increased apoptosis associated with p53 activation.

Development
A FRT site flanked PGK-Neo cassette containing a loxP site, was inserted downstream of exon 6 of the targeted gene, and another loxP site was inserted upstream of exon 5. This construct was electroporated into unspecified 129 derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to mice expressing FLP recombinase under the control of the human beta actin promoter, B6;SJL-Tg(ACTFLPe)9205Dym/J (Stock No. 003800). Mice that retained the loxP site flanked exons 5 and 6 were then backcrossed to C57BL/6 for 5 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Foxn1
000820   AKR/J-Foxn1nu-str/J
018448   B6(Cg)-Foxn1tm3(cre)Nrm/J
016195   B6(SJL)-Foxn1nu-2J/GrsrJ
000521   B6.AK-Foxn1nu-str/J
000819   B6.Cg-Foxn1nu/J
000711   CByJ.Cg-Foxn1nu/J
007850   J:NU
002019   NU/J
003118   STOCK Ces1ce Foxn1nu/J
View Strains carrying other alleles of Foxn1     (9 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
T-Cell Immunodeficiency, Congenital Alopecia, and Nail Dystrophy   (FOXN1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Foxn1tm1.1Dmsu/Foxn1tm1.1Dmsu

        involves: 129/Sv * C57BL/6 * SJL
  • normal phenotype
  • no abnormal phenotype detected
    • mice are normal   (MGI Ref ID J:159773)

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Foxn1tm1.1Dmsu/Foxn1tm1.1Dmsu Tg(CAG-cre/Esr1*)5Amc/0

        involves: 129/Sv * C57BL/6 * CBA * SJL   (conditional)
  • immune system phenotype
  • abnormal thymus morphology
    • following tamoxifen treatment, mice exhibit a decrease in thymic stromal cells compared to in wild-type mice   (MGI Ref ID J:159773)
    • decreased thymocyte number
      • following tamoxifen treatment   (MGI Ref ID J:159773)
    • small thymus
      • following tamoxifen treatment   (MGI Ref ID J:159773)
      • thymus atrophy
        • following tamoxifen treatment   (MGI Ref ID J:159773)
  • decreased double-positive T cell number
    • following tamoxifen treatment   (MGI Ref ID J:159773)
  • hematopoietic system phenotype
  • abnormal thymus morphology
    • following tamoxifen treatment, mice exhibit a decrease in thymic stromal cells compared to in wild-type mice   (MGI Ref ID J:159773)
    • decreased thymocyte number
      • following tamoxifen treatment   (MGI Ref ID J:159773)
    • small thymus
      • following tamoxifen treatment   (MGI Ref ID J:159773)
      • thymus atrophy
        • following tamoxifen treatment   (MGI Ref ID J:159773)
  • decreased double-positive T cell number
    • following tamoxifen treatment   (MGI Ref ID J:159773)
  • endocrine/exocrine gland phenotype
  • abnormal thymus morphology
    • following tamoxifen treatment, mice exhibit a decrease in thymic stromal cells compared to in wild-type mice   (MGI Ref ID J:159773)
    • decreased thymocyte number
      • following tamoxifen treatment   (MGI Ref ID J:159773)
    • small thymus
      • following tamoxifen treatment   (MGI Ref ID J:159773)
      • thymus atrophy
        • following tamoxifen treatment   (MGI Ref ID J:159773)

Foxn1tm1.1Dmsu/Foxn1tm1.1Dmsu Tg(KRT5-cre/ERT2)2Ipc/0

        involves: 129/Sv * C57BL/6 * SJL   (conditional)
  • immune system phenotype
  • abnormal thymus corticomedullary boundary morphology
    • following tamoxifen treatment, the medullary region architecture is altered and the corticomedullary junction becomes indistinct compared to in wild-type mice   (MGI Ref ID J:159773)
  • abnormal thymus medulla morphology
    • following tamoxifen treatment, the medullary region architecture is altered and the corticomedullary junction becomes indistinct compared to in wild-type mice   (MGI Ref ID J:159773)
  • decreased thymocyte number
    • following tamoxifen treatment   (MGI Ref ID J:159773)
  • small thymus
    • following tamoxifen treatment   (MGI Ref ID J:159773)
    • thymus atrophy
      • following tamoxifen treatment   (MGI Ref ID J:159773)
  • hematopoietic system phenotype
  • abnormal thymus corticomedullary boundary morphology
    • following tamoxifen treatment, the medullary region architecture is altered and the corticomedullary junction becomes indistinct compared to in wild-type mice   (MGI Ref ID J:159773)
  • abnormal thymus medulla morphology
    • following tamoxifen treatment, the medullary region architecture is altered and the corticomedullary junction becomes indistinct compared to in wild-type mice   (MGI Ref ID J:159773)
  • decreased thymocyte number
    • following tamoxifen treatment   (MGI Ref ID J:159773)
  • small thymus
    • following tamoxifen treatment   (MGI Ref ID J:159773)
    • thymus atrophy
      • following tamoxifen treatment   (MGI Ref ID J:159773)
  • endocrine/exocrine gland phenotype
  • abnormal thymus corticomedullary boundary morphology
    • following tamoxifen treatment, the medullary region architecture is altered and the corticomedullary junction becomes indistinct compared to in wild-type mice   (MGI Ref ID J:159773)
  • abnormal thymus medulla morphology
    • following tamoxifen treatment, the medullary region architecture is altered and the corticomedullary junction becomes indistinct compared to in wild-type mice   (MGI Ref ID J:159773)
  • decreased thymocyte number
    • following tamoxifen treatment   (MGI Ref ID J:159773)
  • small thymus
    • following tamoxifen treatment   (MGI Ref ID J:159773)
    • thymus atrophy
      • following tamoxifen treatment   (MGI Ref ID J:159773)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Foxn1tm1.1Dmsu
Allele Name targeted mutation 1.1, Dong-Ming Su
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) FoxN1fx;
Mutation Made By Dong-Ming Su,   Univ. of TX Health Science Center Tyler
Strain of Origin129
Gene Symbol and Name Foxn1, forkhead box N1
Chromosome 11
Gene Common Name(s) D11Bhm185e; DNA segment, Chr 11, Boehm 185, expressed; FKHL20; HNF-3/forkhead homolog 11; Hfh11; RONU; Rnu; WHN; nu; nude;
Molecular Note Flp mediated recombination removed the neo cassette leaving exons 5 and 6 floxed. [MGI Ref ID J:159773]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Cheng L; Guo J; Sun L; Fu J; Barnes PF; Metzger D; Chambon P; Oshima RG; Amagai T; Su DM. 2010. Postnatal tissue-specific disruption of transcription factor FoxN1 triggers acute thymic atrophy. J Biol Chem 285(8):5836-47. [PubMed: 19955175]  [MGI Ref ID J:159773]

Additional References

Foxn1tm1.1Dmsu related

Burnley P; Rahman M; Wang H; Zhang Z; Sun X; Zhuge Q; Su DM. 2013. Role of the p63-FoxN1 regulatory axis in thymic epithelial cell homeostasis during aging. Cell Death Dis 4:e932. [PubMed: 24263106]  [MGI Ref ID J:205598]

Guo J; Rahman M; Cheng L; Zhang S; Tvinnereim A; Su DM. 2011. Morphogenesis and maintenance of the 3D thymic medulla and prevention of nude skin phenotype require FoxN1 in pre- and post-natal K14 epithelium. J Mol Med (Berl) 89(3):263-77. [PubMed: 21109991]  [MGI Ref ID J:187504]

Huang W; Qi Q; Hu J; Huang F; Laufer TM; August A. 2014. Dendritic cell-MHC class II and Itk regulate functional development of regulatory innate memory CD4+ T cells in bone marrow transplantation. J Immunol 192(7):3435-41. [PubMed: 24610010]  [MGI Ref ID J:210245]

Xia J; Wang H; Guo J; Zhang Z; Coder B; Su DM. 2012. Age-Related Disruption of Steady-State Thymic Medulla Provokes Autoimmune Phenotype via Perturbing Negative Selection. Aging Dis 3(3):248-59. [PubMed: 22724083]  [MGI Ref ID J:196234]

Zhang Z; Burnley P; Coder B; Su DM. 2012. Insights on FoxN1 biological significance and usages of the "nude" mouse in studies of T-lymphopoiesis. Int J Biol Sci 8(8):1156-67. [PubMed: 23091413]  [MGI Ref ID J:202298]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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