Strain Name:

C3.B6-Erbb3m3329Ddg/J

Stock Number:

013059

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Mice homozygous for the Erbb3 (v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)) I449S loss-of-function mutation are embryonic lethal at day 14.5 (E14.5). Mutant embryos exhibit extreme defasciculation of all major peripheral branches of the dorsal root ganglia (DRG). Additionally, mutant embryos have complete loss of the mandibular branch of the trigeminal ganglia with defasciculation of the ophthalmic branch. This strain may be useful in studies of nerve development.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names C3.B6-Erbb3I449S/DdgJ    (Changed: 21-JUN-10 )
Type Chemically Induced Mutation; Congenic; Mutant Strain;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN9pN1
Generation Definitions
 
Donating Investigator David D. Ginty,   Harvard Medical School

Description
Mice homozygous for the Erbb3 (v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)) I449S loss-of-function mutation are embryonic lethal at day 14.5 (E14.5). Mutant embryos exhibit extreme defasciculation of all major peripheral branches of the dorsal root ganglia (DRG). While the major dorsal and lateral projections form and are dissociated, all of the secondary and tertiary branches are absent. Upon reaching their superficial target, the axonal projections are splayed out almost randomly within the dermatome. Additionally, mutant embryos have complete loss of the mandibular branch of the trigeminal ganglia with defasciculation of the ophthalmic branch. This strain may be useful in studies of nerve development.

Development
ENU (N-ethyl-N-nitrosourea) mutagenesis carried out on a C57BL/6 background was generated a single point mutation in exon 13 (T1346G), creating a single amino acid substitution (I449S). This strain was backcrossed to a C3H/HeJ strain for 9 generation by the donating laboratory. This is line 3329.

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Lethal Congenital Contracture Syndrome 2; LCCS2   (ERBB3)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Erbb3m3329Ddg/Erbb3m3329Ddg

        involves: C57BL/6
  • mortality/aging
  • complete prenatal lethality
    • while present in Mendelian ratio at E11.5, only one mouse was found at E14.5 and no mice are present at P0   (MGI Ref ID J:160880)
  • partial embryonic lethality during organogenesis
    • while present in Mendelian ratio at E11.5, only one mouse was found at E14.5 and no mice are present at P0   (MGI Ref ID J:160880)
  • nervous system phenotype
  • abnormal axon fasciculation
    • mice exhibit defasciculation of all the major peripheral branches of the dorsal root ganglia and the trigeminal and spinal nerves compared with wild-type mice   (MGI Ref ID J:160880)
  • abnormal dorsal root ganglion morphology
    • mice exhibit defasciculation of all the major peripheral branches of the dorsal root ganglia compared with wild-type mice   (MGI Ref ID J:160880)
    • secondary and tertiary branches are absent with axonal projections splaying out randomly within the dermatome unlike in wild-type mice   (MGI Ref ID J:160880)
  • abnormal mandibular nerve morphology
    • mice exhibit an almost complete loss of the mandibular branch of the trigeminal ganglia unlike in wild-type mice   (MGI Ref ID J:160880)
  • abnormal ophthalmic nerve morphology
    • mice exhibit defasciculation of the ophthalmic branch of the trigeminal ganglia compared with wild-type mice   (MGI Ref ID J:160880)
  • abnormal spinal nerve morphology
    • mice exhibit defasciculation of spinal nerves compared with wild-type mice   (MGI Ref ID J:160880)
  • cellular phenotype
  • abnormal axon fasciculation
    • mice exhibit defasciculation of all the major peripheral branches of the dorsal root ganglia and the trigeminal and spinal nerves compared with wild-type mice   (MGI Ref ID J:160880)

Erbb3m3329Ddg/Erbb3m3329Ddg

        involves: C3H/He * C57BL/6
  • nervous system phenotype
  • abnormal nervous system morphology
    • mice exhibit abnormal development and patterning of the peripheral nervous system compared with wild-type mice   (MGI Ref ID J:159834)
    • abnormal axon fasciculation
      • mice exhibit defasciculation of all major peripheral branches of the dorsal root ganglia compared with wild-type mice   (MGI Ref ID J:159834)
    • abnormal dorsal root ganglion morphology
      • mice exhibit defasciculation of all major peripheral branches of the dorsal root ganglia compared with wild-type mice   (MGI Ref ID J:159834)
      • secondary and tertiary branches are absent unlike in wild-type mice   (MGI Ref ID J:159834)
      • axonal projections are splayed out almost randomly within the dermatome unlike in wild-type mice   (MGI Ref ID J:159834)
  • cellular phenotype
  • abnormal axon fasciculation
    • mice exhibit defasciculation of all major peripheral branches of the dorsal root ganglia compared with wild-type mice   (MGI Ref ID J:159834)

Erbb3m3329Ddg/Erbb3tm1Gne

        involves: 129S2/SvPas * C57BL/6
  • nervous system phenotype
  • abnormal axon fasciculation
    • mice exhibit the same defasciculation in trigeminal and spinal nerves observed in either single homozygote   (MGI Ref ID J:160880)
  • abnormal spinal nerve morphology
    • mice exhibit the same defasciculation in spinal nerves observed in either single homozygote   (MGI Ref ID J:160880)
  • abnormal trigeminal nerve morphology
    • mice exhibit the same defasciculation in the trigeminal nerve observed in either single homozygote   (MGI Ref ID J:160880)
  • cellular phenotype
  • abnormal axon fasciculation
    • mice exhibit the same defasciculation in trigeminal and spinal nerves observed in either single homozygote   (MGI Ref ID J:160880)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Embryonic Lethality (Homozygous)
Neurodevelopmental Defects

Neurobiology Research
Neurodevelopmental Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Erbb3m3329Ddg
Allele Name mutation 3329, David D Ginty
Allele Type Chemically induced (ENU)
Common Name(s) ErbB3I449S;
Strain of OriginC57BL/6
Gene Symbol and Name Erbb3, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
Chromosome 10
Gene Common Name(s) C76256; ErbB-3; Erbb-3; Erbb3r; HER3; LCCS2; MDA-BF-1; avian erythroblastosis oncogene B 3; avian erythroblastosis oncogene B 3 receptor; c-erbB-3; c-erbB3; erbB3-S; expressed sequence C76256; nuc-ErbB3; p180-ErbB3; p45-sErbB3; p85-sErbB3;
Molecular Note ENU (N-ethyl-N-nitrosourea) mutagenesis generated a single point mutation in exon 13 (T1346G), creating a single amino acid substitution of serine for isoleucine at position 449 (I449S). [MGI Ref ID J:160880]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Merte J; Wang Q; Vander Kooi CW; Sarsfield S; Leahy DJ; Kolodkin AL; Ginty DD. 2010. A forward genetic screen in mice identifies Sema3A(K108N), which binds to neuropilin-1 but cannot signal. J Neurosci 30(16):5767-75. [PubMed: 20410128]  [MGI Ref ID J:159834]

Additional References

Erbb3m3329Ddg related

Merte J. 2010. A mouse forward-genetic screen for novel loci critical for neural development MGI Direct Data Submission :.  [MGI Ref ID J:160880]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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