Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names C3.B6-Erbb3I449S/DdgJ    (Changed: 21-JUN-10 ) Type Chemically Induced Mutation; Congenic; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N9pN1 Generation Definitions   Donating Investigator David D. Ginty,   Johns Hopkins University

Description
Mice homozygous for the Erbb3 (v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)) I449S loss-of-function mutation are embryonic lethal at day 14.5 (E14.5). Mutant embryos exhibit extreme defasciculation of all major peripheral branches of the dorsal root ganglia (DRG). While the major dorsal and lateral projections form and are dissociated, all of the secondary and tertiary branches are absent. Upon reaching their superficial target, the axonal projections are splayed out almost randomly within the dermatome. Additionally, mutant embryos have complete loss of the mandibular branch of the trigeminal ganglia with defasciculation of the ophthalmic branch. This strain may be useful in studies of nerve development.

Development
ENU (N-ethyl-N-nitrosourea) mutagenesis carried out on a C57BL/6 background was generated a single point mutation in exon 13 (T1346G), creating a single amino acid substitution (I449S). This strain was backcrossed to a C3H/HeJ strain for 9 generation by the donating laboratory. This is line 3329.

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Lethal Congenital Contracture Syndrome 2; LCCS2   (ERBB3)

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Erbb3^m3329Ddg/Erbb3^m3329Ddg

        involves: C57BL/6

• mortality/aging
• complete prenatal lethality
  • while present in Mendelian ratio at E11.5, only one mouse was found at E14.5 and no mice are present at P0   (MGI Ref ID J:160880)
• partial embryonic lethality during organogenesis
  • while present in Mendelian ratio at E11.5, only one mouse was found at E14.5 and no mice are present at P0   (MGI Ref ID J:160880)
• nervous system phenotype
• abnormal axon fasiculation
  • mice exhibit defasciculation of all the major peripheral branches of the dorsal root ganglia and the trigeminal and spinal nerves compared with wild-type mice   (MGI Ref ID J:160880)
• abnormal dorsal root ganglion morphology
  • mice exhibit defasciculation of all the major peripheral branches of the dorsal root ganglia compared with wild-type mice   (MGI Ref ID J:160880)
  • secondary and tertiary branches are absent with axonal projections splaying out randomly within the dermatome unlike in wild-type mice   (MGI Ref ID J:160880)
• abnormal mandibular nerve morphology
  • mice exhibit an almost complete loss of the mandibular branch of the trigeminal ganglia unlike in wild-type mice   (MGI Ref ID J:160880)
• abnormal ophthalmic nerve morphology
  • mice exhibit defasciculation of the ophthalmic branch of the trigeminal ganglia compared with wild-type mice   (MGI Ref ID J:160880)
• abnormal spinal nerve morphology
  • mice exhibit defasciculation of spinal nerves compared with wild-type mice   (MGI Ref ID J:160880)
• cellular phenotype
• abnormal axon fasiculation
  • mice exhibit defasciculation of all the major peripheral branches of the dorsal root ganglia and the trigeminal and spinal nerves compared with wild-type mice   (MGI Ref ID J:160880)

Erbb3^m3329Ddg/Erbb3^m3329Ddg

        involves: C3H/He * C57BL/6

• nervous system phenotype
• abnormal nervous system morphology
  • mice exhibit abnormal development and patterning of the peripheral nervous system compared with wild-type mice   (MGI Ref ID J:159834)
• • abnormal axon fasiculation
    • mice exhibit defasciculation of all major peripheral branches of the dorsal root ganglia compared with wild-type mice   (MGI Ref ID J:159834)
  • abnormal dorsal root ganglion morphology
    • mice exhibit defasciculation of all major peripheral branches of the dorsal root ganglia compared with wild-type mice   (MGI Ref ID J:159834)
    • secondary and tertiary branches are absent unlike in wild-type mice   (MGI Ref ID J:159834)
    • axonal projections are splayed out almost randomly within the dermatome unlike in wild-type mice   (MGI Ref ID J:159834)
• cellular phenotype
• abnormal axon fasiculation
  • mice exhibit defasciculation of all major peripheral branches of the dorsal root ganglia compared with wild-type mice   (MGI Ref ID J:159834)

Erbb3^m3329Ddg/Erbb3^tm1Gne

        involves: 129S2/SvPas * C57BL/6

• nervous system phenotype
• abnormal axon fasiculation
  • mice exhibit the same defasciculation in trigeminal and spinal nerves observed in either single homozygote   (MGI Ref ID J:160880)
• abnormal spinal nerve morphology
  • mice exhibit the same defasciculation in spinal nerves observed in either single homozygote   (MGI Ref ID J:160880)
• abnormal trigeminal nerve morphology
  • mice exhibit the same defasciculation in the trigeminal nerve observed in either single homozygote   (MGI Ref ID J:160880)
• cellular phenotype
• abnormal axon fasiculation
  • mice exhibit the same defasciculation in trigeminal and spinal nerves observed in either single homozygote   (MGI Ref ID J:160880)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Embryonic Lethality (Homozygous)
Neurodevelopmental Defects

Neurobiology Research
Neurodevelopmental Defects

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Erbb3m3329Ddg
Allele Name		mutation 3329, David D Ginty
Allele Type		Chemically induced (ENU)
Common Name(s)		ErbB3I449S;
Strain of Origin		C57BL/6
Gene Symbol and Name		Erbb3, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
Chromosome		10
Gene Common Name(s)		C76256; ErbB-3; Erbb-3; Erbb3r; HER3; LCCS2; MDA-BF-1; avian erythroblastosis oncogene B 3; avian erythroblastosis oncogene B 3 receptor; c-erbB-3; c-erbB3; erbB3-S; expressed sequence C76256; nuc-ErbB3; p180-ErbB3; p45-sErbB3; p85-sErbB3;
Molecular Note		ENU (N-ethyl-N-nitrosourea) mutagenesis generated a single point mutation in exon 13 (T1346G), creating a single amino acid substitution of serine for isoleucine at position 449 (I449S). [MGI Ref ID J:160880]

Genotyping

Genotyping Information

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Merte J; Wang Q; Vander Kooi CW; Sarsfield S; Leahy DJ; Kolodkin AL; Ginty DD. 2010. A forward genetic screen in mice identifies Sema3A(K108N), which binds to neuropilin-1 but cannot signal. J Neurosci 30(16):5767-75. [PubMed: 20410128]  [MGI Ref ID J:159834]

Additional References

Erbb3^m3329Ddg related

Merte J. 2010. A mouse forward-genetic screen for novel loci critical for neural development MGI Direct Data Submission :.  [MGI Ref ID J:160880]

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