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| This strain carries a targeted mutation of the Tas2r105 (taste receptor, type 2, member 105; also known as T2R5) gene which encodes a cycloheximide-specific G-protein coupled receptor associated with bitter taste sensing. Homozygotes selectively lack electrophysiological responses to the taste of cycloheximide. This strain may be useful in studies of taste and chemosensation. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F?pN1
Generation DefinitionsDonating Investigator Charles Zuker, Columbia University Description
This strain carries a targeted mutation of the Tas2r105 (taste receptor, type 2, member 105; also known as T2R5) gene which encodes a cycloheximide-specific G-protein coupled receptor associated with bitter taste sensing. Homozygotes selectively lack electrophysiological responses to the taste of cycloheximide, but not to other bitter substances tested. Responses to sweet, umami, salty and sour tastants are not affected. Homozygous mice lack expression of the targeted gene and have normal viability, body weight, overall anatomy and general behavior. Taste receptor cells appear normal morphologically and numerically. This strain may be useful in studies of taste and chemosensation.Development
The entire coding sequence of the targeted gene was replaced by a reverse-tetracycline dependent transactivator (rtTA) (which is non-functional) and loxP-flanked PGK-neomycin resistance cassette. Homologous recombination was carried out in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. This strain was backcrossed two times to C57BL/6 by the donating laboratory.
Phenotype
Phenotype Information
View Mammalian Phenotype Terms
Mammalian Phenotype Terms provided by MGI
assigned by genotype
Tas2r105tm2Csz/Tas2r105tm2Csz
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
- taste/olfaction phenotype
- abnormal bitter taste sensitivity
- strong and selective impairment in ability to taste cycloheximide (a bitter tastant), however responses to sweet, umami, sour and salty tastants were similar to wildtype (MGI Ref ID J:97593)
- animals are not behaviorally averse to cycloheximide, even at concentrations 100-fold higher than those required to trigger a strong repulsion in wildtype (MGI Ref ID J:97593)
This mouse can be used to support research in many areas including:
Neurobiology Research
Receptor Defects
G protein-coupled receptor
Sensorineural Research
Taste Defects
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tas2r105tm2Csz | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Charles S Zuker | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | T2R5-; | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Gene Symbol and Name | Tas2r105, taste receptor, type 2, member 105 | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | T2R05; T2R9; T2r5; Tas2r5; mGR05; mt2r5; | ||
| Molecular Note | A reverse-tetracycline dependent transactivator and a floxed PGK-neo replaced the entire coding region. [MGI Ref ID J:97593] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tas2r105tm2Csz, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Mueller KL; Hoon MA; Erlenbach I; Chandrashekar J; Zuker CS; Ryba NJ. 2005. The receptors and coding logic for bitter taste. Nature 434(7030):225-9. [PubMed: 15759003] [MGI Ref ID J:97593]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintained as a live colony, homozygotes or heterozygotes may be bred.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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