Strain Name:

B6;129-Trpm5tm1Csz/J

Stock Number:

013068

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
This strain carries a targeted mutation of the Trpm5 (transient receptor potential cation channel, subfamily M, member 5) gene which encodes a taste transient receptor potential (TRP) ion channel. Sweet, amino acid, and bitter taste reception is abolished in homozygotes, but sour and salty tastes are not impacted. This strain may be useful in studies of taste, olfaction, chemosensation and nutrient sensing.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
Generation+pN1
Generation Definitions
 
Donating Investigator Charles Zuker,   Columbia University

Description
This strain carries a targeted mutation of the Trpm5 (transient receptor potential cation channel, subfamily M, member 5) gene which encodes a taste transient receptor potential (TRP) ion channel. Sweet, amino acid, and bitter taste reception is abolished in homozygotes, but sour and salty tastes are not impacted. Homozygous mice lack expression of the gene and have normal viability, body weight, overall anatomy and general behavior. Taste receptor cells appear normal morphologically and numerically. This strain may be useful in studies of taste, olfaction, chemosensation and nutrient sensing.

Development
Exons 15-19, encoding the first five transmembrane domains, were replaced by a PGK-neomycin resistance cassette in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. This strain was backcrossed to C57BL/6 for two generations by the donating laboratory.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Trpm5
005848   B6.129P2-Trpm5tm1Dgen/J
View Strains carrying other alleles of Trpm5     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Trpm5tm1Csz/Trpm5tm1Csz

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6
  • taste/olfaction phenotype
  • abnormal gustatory system physiology
    • nerve responses to sweet, amino acid, and bitter stimuli were abolished   (MGI Ref ID J:82314)
    • normal nerve responses to salty or sour stimuli   (MGI Ref ID J:82314)
    • abnormal taste sensitivity
      • preference for sweet, amino acid or bitter vs. water abolished   (MGI Ref ID J:82314)
      • preference for salty or sour vs. water similar to controls   (MGI Ref ID J:82314)
      • abnormal bitter taste sensitivity   (MGI Ref ID J:82314)
      • abnormal sweet taste sensitivity   (MGI Ref ID J:82314)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Channel and Transporter Defects

Neurobiology Research
Channel and Transporter Defects

Sensorineural Research
Taste Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Trpm5tm1Csz
Allele Name targeted mutation 1, Charles S Zuker
Allele Type Targeted (Null/Knockout)
Mutation Made By Charles Zuker,   Columbia University
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Trpm5, transient receptor potential cation channel, subfamily M, member 5
Chromosome 7
Gene Common Name(s) 9430099A16Rik; LTRPC5; MTR1; RIKEN cDNA 9430099A16 gene;
Molecular Note Exons 15 through 19 were replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region encoded 5 transmembrane domains. Immunostaining demonstrated an absence of normal protein in homozygous mutant taste buds. [MGI Ref ID J:82314]

Genotyping

Genotyping Information

Genotyping Protocols

Trpm5tm1Csz, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Zhang Y; Hoon MA; Chandrashekar J; Mueller KL; Cook B; Wu D; Zuker CS; Ryba NJ. 2003. Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways. Cell 112(3):293-301. [PubMed: 12581520]  [MGI Ref ID J:82314]

Additional References

Trpm5tm1Csz related

Barbet G; Demion M; Moura IC; Serafini N; Leger T; Vrtovsnik F; Monteiro RC; Guinamard R; Kinet JP; Launay P. 2008. The calcium-activated nonselective cation channel TRPM4 is essential for the migration but not the maturation of dendritic cells. Nat Immunol 9(10):1148-56. [PubMed: 18758465]  [MGI Ref ID J:140991]

Colsoul B; Schraenen A; Lemaire K; Quintens R; Van Lommel L; Segal A; Owsianik G; Talavera K; Voets T; Margolskee RF; Kokrashvili Z; Gilon P; Nilius B; Schuit FC; Vennekens R. 2010. Loss of high-frequency glucose-induced Ca2+ oscillations in pancreatic islets correlates with impaired glucose tolerance in Trpm5-/- mice. Proc Natl Acad Sci U S A 107(11):5208-13. [PubMed: 20194741]  [MGI Ref ID J:158750]

Domingos AI; Sordillo A; Dietrich MO; Liu ZW; Tellez LA; Vaynshteyn J; Ferreira JG; Ekstrand MI; Horvath TL; de Araujo IE; Friedman JM. 2013. Hypothalamic melanin concentrating hormone neurons communicate the nutrient value of sugar. Elife 2:e01462. [PubMed: 24381247]  [MGI Ref ID J:206211]

Huang YA; Roper SD. 2010. Intracellular Ca(2+) and TRPM5-mediated membrane depolarization produce ATP secretion from taste receptor cells. J Physiol 588(Pt 13):2343-50. [PubMed: 20498227]  [MGI Ref ID J:176764]

Kyriazis GA; Soundarapandian MM; Tyrberg B. 2012. Sweet taste receptor signaling in beta cells mediates fructose-induced potentiation of glucose-stimulated insulin secretion. Proc Natl Acad Sci U S A 109(8):E524-32. [PubMed: 22315413]  [MGI Ref ID J:182009]

Oliveira-Maia AJ; Stapleton-Kotloski JR; Lyall V; Phan TH; Mummalaneni S; Melone P; Desimone JA; Nicolelis MA; Simon SA. 2009. Nicotine activates TRPM5-dependent and independent taste pathways. Proc Natl Acad Sci U S A 106(5):1596-601. [PubMed: 19164511]  [MGI Ref ID J:144486]

Ren X; Ferreira JG; Zhou L; Shammah-Lagnado SJ; Yeckel CW; de Araujo IE. 2010. Nutrient selection in the absence of taste receptor signaling. J Neurosci 30(23):8012-23. [PubMed: 20534849]  [MGI Ref ID J:160888]

Ren Z; Rhyu MR; Phan TH; Mummalaneni S; Murthy KS; Grider JR; DeSimone JA; Lyall V. 2013. TRPM5-dependent amiloride- and benzamil-insensitive NaCl chorda tympani taste nerve response. Am J Physiol Gastrointest Liver Physiol 305(1):G106-17. [PubMed: 23639808]  [MGI Ref ID J:202786]

Rong M; He W; Yasumatsu K; Kokrashvili Z; Perez CA; Mosinger B; Ninomiya Y; Margolskee RF; Damak S. 2005. Signal Transduction of Umami Taste: Insights from Knockout Mice. Chem Senses 30(suppl_1):i33-i34. [PubMed: 15738181]  [MGI Ref ID J:104626]

de Araujo IE; Oliveira-Maia AJ; Sotnikova TD; Gainetdinov RR; Caron MG; Nicolelis MA; Simon SA. 2008. Food reward in the absence of taste receptor signaling. Neuron 57(6):930-41. [PubMed: 18367093]  [MGI Ref ID J:136043]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, homozygotes or heterozygotes may be bred.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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