Strain Name:

B6.129S-Pbx1tm3.1Mlc/J

Stock Number:

013085

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These Pbx1f/f mutant mice possess loxP sites flanking exon 3 of the targeted pre B-cell leukemia transcription factor 1 (Pbx1) gene. This strain may be useful for studying the effect of Pbx1 on the maintenance and development of multiple tissue lineages.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.129S6-Pbx1tm3.1Mlc/J    (Changed: 21-JUL-10 )
B6J.Cg-Pbx1tm3.1Mlc/J    (Changed: 21-JUL-10 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating Investigator Michael L Cleary,   Stanford University School of Medicine

Description
These Pbx1f/f mutant mice possess loxP sites flanking exon 3 of the targeted pre B-cell leukemia transcription factor 1 (Pbx1) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues. This strain may be useful for studying the effect of Pbx1 on the maintenance and development of multiple tissue lineages.

For example, when crossed to a strain expressing interferon-inducible Cre recombinase in multiple tissues (see Stock No. 003556), this mutant mouse strain may be useful in studies of hematopoietic stem cell renewal.

When crossed to a strain expressing Cre recombinase in endothelial cells (see Stock No. 008863), this mutant mouse strain may be useful in studies of studies of hematopoietic stem cell renewal.

Development
A targeting vector was designed to insert a loxP site upstream of exon 3, and a second loxP site downstream of exon 3 of the pre B-cell leukemia transcription factor 1 (Pbx1) gene. The floxed exon was followed by a frt-flanked resistance cassette. The construct was electroporated into 129S6/SvEvTac-derived embryonic stem (ES) cells and correctly targeted ES cells were injected into blastocysts. Offspring, heterozygous for this Pbx1 allele, were bred with 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym transgenic mice to delete the resistance cassette. Progeny were crossed to remove the Flp-expressing mutation, resulting in a colony homozygous for the floxed-Pbx1 (Pbx1f/f) allele. These mice were backcrossed to C57BL/6J mice for at least 14 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664 for at least one generation to establish the colony.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
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Pbx1tm1Mlc/Pbx1tm3.1Mlc Tg(Mx1-cre)1Cgn/0

        involves: 129S6/SvEvTac * C57BL/6 * CBA   (conditional)
  • hematopoietic system phenotype
  • abnormal hematopoiesis
    • abnormal common lymphocyte progenitor cell morphology
      • reduction in the number of common lymphoid progenitor cells   (MGI Ref ID J:149799)
  • abnormal hematopoietic stem cell morphology
    • assays indicate that long term hematopoietic stem cells are impaired in self renewal   (MGI Ref ID J:149799)
    • decreased hematopoietic stem cell number
      • significant reduction in the number of long term hematopoietic stem cells   (MGI Ref ID J:149799)
  • immune system phenotype
  • abnormal common lymphocyte progenitor cell morphology
    • reduction in the number of common lymphoid progenitor cells   (MGI Ref ID J:149799)

Pbx1tm1Mlc/Pbx1tm3.1Mlc Tg(Tek-cre)1Ywa/0

        involves: 129S6/SvEvTac * C57BL/6 * SJL   (conditional)
  • hematopoietic system phenotype
  • abnormal hematopoiesis
    • slight decrease in the number of granulocyte-monocyte progenitor (GMP) cells   (MGI Ref ID J:149799)
    • decrease in the frequency of colony forming cells in semi solid culture   (MGI Ref ID J:149799)
    • reduction in stem cell and progenitor cell numbers increases progressively with age starting around 2 weeks of age   (MGI Ref ID J:149799)
    • in competitive repopulation assays mutant bone marrow cells are unable to compete with wild-type cells   (MGI Ref ID J:149799)
    • cells can engraft under noncompetitive conditions but show long term progressive graft failure   (MGI Ref ID J:149799)
    • abnormal common lymphocyte progenitor cell morphology
      • marked reduction in the number of common lymphoid progenitor cells   (MGI Ref ID J:149799)
    • decreased B cell number
      • in the bone marrow   (MGI Ref ID J:149799)
      • decreased pre-B cell number
        • of the subpopulations of B cells, pre-B cells are the most reduced in number   (MGI Ref ID J:149799)
    • decreased T cell number
      • all T cell lineages are reduced in number in the thymus starting from the earliest immature DN1 stage   (MGI Ref ID J:149799)
      • decreased double-negative T cell number
        • decrease in the number of DN1 cells   (MGI Ref ID J:149799)
    • decreased common myeloid progenitor cell number
      • mild decrease   (MGI Ref ID J:149799)
    • decreased pro-B cell number
      • decrease in B cell numbers is first significant at the pro-B stage   (MGI Ref ID J:149799)
  • abnormal hematopoietic stem cell morphology
    • increase in proliferation of long term hematopoietic stem cells suggesting a decrease in the pool of quiescent stem cells   (MGI Ref ID J:149799)
    • secondary transplant assays indicate that long term hematopoietic stem cells are impaired in self renewal   (MGI Ref ID J:149799)
    • decreased hematopoietic stem cell number
      • marked reduction in the Lin-c-Kit+Sca-1+ cell population   (MGI Ref ID J:149799)
      • significant reduction in the number of long term hematopoietic stem cells   (MGI Ref ID J:149799)
  • abnormal spleen morphology
    • pronounced histological disorganization is seen in young mice   (MGI Ref ID J:149799)
    • small spleen
      • seen in young mice   (MGI Ref ID J:149799)
      • spleen hypoplasia
        • decrease in size is mainly the result of a decrease in the number of CD45+ hematopoietic cells   (MGI Ref ID J:149799)
  • abnormal thymus morphology
    • pronounced histological disorganization is seen in young mice   (MGI Ref ID J:149799)
    • small thymus
      • seen in young mice   (MGI Ref ID J:149799)
      • thymus hypoplasia
        • decrease in size is mainly the result of a decrease in the number of CD45+ hematopoietic cells   (MGI Ref ID J:149799)
  • decreased bone marrow cell number
    • hypocellular mostly as a result of a decrease in the numbers of B lineage cells   (MGI Ref ID J:149799)
  • immune system phenotype
  • abnormal common lymphocyte progenitor cell morphology
    • marked reduction in the number of common lymphoid progenitor cells   (MGI Ref ID J:149799)
  • abnormal spleen morphology
    • pronounced histological disorganization is seen in young mice   (MGI Ref ID J:149799)
    • small spleen
      • seen in young mice   (MGI Ref ID J:149799)
      • spleen hypoplasia
        • decrease in size is mainly the result of a decrease in the number of CD45+ hematopoietic cells   (MGI Ref ID J:149799)
  • abnormal thymus morphology
    • pronounced histological disorganization is seen in young mice   (MGI Ref ID J:149799)
    • small thymus
      • seen in young mice   (MGI Ref ID J:149799)
      • thymus hypoplasia
        • decrease in size is mainly the result of a decrease in the number of CD45+ hematopoietic cells   (MGI Ref ID J:149799)
  • decreased B cell number
    • in the bone marrow   (MGI Ref ID J:149799)
    • decreased pre-B cell number
      • of the subpopulations of B cells, pre-B cells are the most reduced in number   (MGI Ref ID J:149799)
  • decreased T cell number
    • all T cell lineages are reduced in number in the thymus starting from the earliest immature DN1 stage   (MGI Ref ID J:149799)
    • decreased double-negative T cell number
      • decrease in the number of DN1 cells   (MGI Ref ID J:149799)
  • decreased pro-B cell number
    • decrease in B cell numbers is first significant at the pro-B stage   (MGI Ref ID J:149799)
  • endocrine/exocrine gland phenotype
  • abnormal thymus morphology
    • pronounced histological disorganization is seen in young mice   (MGI Ref ID J:149799)
    • small thymus
      • seen in young mice   (MGI Ref ID J:149799)
      • thymus hypoplasia
        • decrease in size is mainly the result of a decrease in the number of CD45+ hematopoietic cells   (MGI Ref ID J:149799)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Signal Transduction

Developmental Biology Research
Internal/Organ Defects
      hematopoietic defects

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Pbx1tm3.1Mlc
Allele Name targeted mutation 3.1, Michael L Cleary
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Pbx1f;
Mutation Made By Michael Cleary,   Stanford University School of Medicine
Strain of Origin129S6/SvEvTac
Gene Symbol and Name Pbx1, pre B cell leukemia homeobox 1
Chromosome 1
Gene Common Name(s) 2310056B04Rik; AI848790; AU015730; D230003C07Rik; Pbx-1; Pbx1a; Pbx1b; RIKEN cDNA 2310056B04 gene; RIKEN cDNA D230003C07 gene; expressed sequence AI848790; expressed sequence AU015730;
Molecular Note A loxP site was inserted upstream of exon 3 and a loxP site and an frt flanked selection cassette were inserted downstream of exon 3 via homologous recombination. The selection cassette was then removed by Flp mediated recombination leaving behind a single frt site. [MGI Ref ID J:149799]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Ficara F; Murphy MJ; Lin M; Cleary ML. 2008. Pbx1 regulates self-renewal of long-term hematopoietic stem cells by maintaining their quiescence. Cell Stem Cell 2(5):484-96. [PubMed: 18462698]  [MGI Ref ID J:149799]

Additional References

Pbx1tm3.1Mlc related

Ficara F; Crisafulli L; Lin C; Iwasaki M; Smith KS; Zammataro L; Cleary ML. 2013. Pbx1 restrains myeloid maturation while preserving lymphoid potential in hematopoietic progenitors. J Cell Sci 126(Pt 14):3181-91. [PubMed: 23660001]  [MGI Ref ID J:205740]

Jackson B; Brown SJ; Avilion AA; O'Shaughnessy RF; Sully K; Akinduro O; Murphy M; Cleary ML; Byrne C. 2011. TALE homeodomain proteins regulate site-specific terminal differentiation, LCE genes and epidermal barrier. J Cell Sci 124(Pt 10):1681-90. [PubMed: 21511732]  [MGI Ref ID J:182945]

Murphy MJ; Polok BK; Schorderet DF; Cleary ML. 2010. Essential role for Pbx1 in corneal morphogenesis. Invest Ophthalmol Vis Sci 51(2):795-803. [PubMed: 19797217]  [MGI Ref ID J:160421]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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