|Please refer to the Mutant Mouse Regional Resource Center (MMRRC) for information about STOCK Mir205tm1Mtm/Mmjax MMRRC Stock Number 034650.|
|These microRNA 205 conditional mutant mice are designed to generate a null allele or a lacZ tagged null allele when combined with Flp or Cre recombinase expressing strains. LacZ expression is observed in pharyngeal arches and limbs at E11.5 and thymus, skin and kidney at E18.5. This mutant mouse strain may be useful in studies of microRNA biology.|
Type Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Mike McManus, University of California, San Francisco
Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous mice die during the perinatal period (E18.5 to postnatal day 1). When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR205 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR205 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). LacZ expression is observed in pharyngeal arches and limbs at E11.5 and thymus, skin and kidney at E18.5. This mutant mouse strain may be useful in studies of microRNA biology.
A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the miR205 stem loop, with one loxP site placed immediately downstream of the miR205 stem loop. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Chimeric mice were crossed with a mouse of mixed genetic background (which incorporated FVB), then crossed to inbred C57BL/6 for two generations. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
|Wild-type from the colony||(approximate)|
|Considerations for Choosing Controls|
View Mammalian Phenotype TermsMammalian Phenotype Terms provided by MGIassigned by genotype
The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.
Mir205tm1Mtm/Mir205tm1Mtminvolves: 129P2/OlaHsd * C57BL/6
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:181752)
View Research ApplicationsResearch ApplicationsThis mouse can be used to support research in many areas including:
Cell Biology Research
loxP-flanked microRNA sequence
lacZ expression in neural tissue
loxP-flanked Sequences: Test/Reporter
loxP-flanked Sequences: Test/Reporter
|Allele Name||targeted mutation 1, Michael McManus|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change, Reporter)|
|Strain of Origin||129P2/OlaHsd|
|Gene Symbol and Name||Mir205, microRNA 205|
|Gene Common Name(s)||Mirn205; mmu-mir-205;|
|Molecular Note||A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA stem loop and one loxP site immediately downstream of the microRNA stem loop. [MGI Ref ID J:181752]|
Farmer DT; Shariat N; Park CY; Liu HJ; Mavropoulos A; McManus MT. 2013. Partially penetrant postnatal lethality of an epithelial specific MicroRNA in a mouse knockout. PLoS One 8(10):e76634. [PubMed: 24116130] [MGI Ref ID J:209028]
Park CY; Jeker LT; Carver-Moore K; Oh A; Liu HJ; Cameron R; Richards H; Li Z; Adler D; Yoshinaga Y; Martinez M; Nefadov M; Abbas AK; Weiss A; Lanier LL; de Jong PJ; Bluestone JA; Srivastava D; McManus MT. 2012. A resource for the conditional ablation of microRNAs in the mouse Cell Rep 1(4):385-91. [PubMed: 22570807] [MGI Ref ID J:181752]
W.M. Keck Center for Noncoding RNAs. 2012. Information obtained from the W.M. Keck Center for Noncoding RNAs Unpublished :. [MGI Ref ID J:181831]
Animal Health ReportsProduction of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Breeding & Husbandry While maintaining a live colony, these mice are bred as heterozygotes. Mice homozygous for the mutation are not viable.
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