Strain Name:

STOCK Mir205tm1Mtm/Mmjax

Availability:

Cryopreserved - Ready for recovery     Available at the JAX MMRRC

Please refer to the Mutant Mouse Regional Resource Center (MMRRC) for information about STOCK Mir205tm1Mtm/Mmjax MMRRC Stock Number 034650.
These microRNA 205 conditional mutant mice are designed to generate a null allele or a lacZ tagged null allele when combined with Flp or Cre recombinase expressing strains. LacZ expression is observed in pharyngeal arches and limbs at E11.5 and thymus, skin and kidney at E18.5. This mutant mouse strain may be useful in studies of microRNA biology.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating Investigator Mike McManus,   University of California, San Francisco

Description
Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous mice die during the perinatal period (E18.5 to postnatal day 1). When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR205 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR205 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). LacZ expression is observed in pharyngeal arches and limbs at E11.5 and thymus, skin and kidney at E18.5. This mutant mouse strain may be useful in studies of microRNA biology.

Development
A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the miR205 stem loop, with one loxP site placed immediately downstream of the miR205 stem loop. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Chimeric mice were crossed with a mouse of mixed genetic background (which incorporated FVB), then crossed to inbred C57BL/6 for two generations. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Control Information

  Control
   Wild-type from the colony (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Mir205tm1Mtm/Mir205tm1Mtm

        involves: 129P2/OlaHsd * C57BL/6
  • no phenotypic analysis
  • *normal* no phenotypic analysis   (MGI Ref ID J:181752)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
microRNA
      loxP-flanked microRNA sequence

Neurobiology Research
lacZ expression in neural tissue
Cre-lox System
      loxP-flanked Sequences: Test/Reporter

Research Tools
Cre-lox System
      loxP-flanked Sequences: Test/Reporter
FLP-FRT System
      FRT-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Mir205tm1Mtm
Allele Name targeted mutation 1, Michael McManus
Allele Type Targeted (Floxed/Frt)
Strain of Origin129P2/OlaHsd
Gene Symbol and Name Mir205, microRNA 205
Chromosome 1
Gene Common Name(s) Mirn205; mmu-mir-205;
Molecular Note A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA stem loop and one loxP site immediately downstream of the microRNA stem loop. [MGI Ref ID J:181752]

Genotyping

Genotyping Information

Genotyping Protocols

Mir205tm1Mtm, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Mir205tm1Mtm related

Park CY; Jeker LT; Carver-Moore K; Oh A; Liu HJ; Cameron R; Richards H; Li Z; Adler D; Yoshinaga Y; Martinez M; Nefadov M; Abbas AK; Weiss A; Lanier LL; de Jong PJ; Bluestone JA; Srivastava D; McManus MT. 2012. A resource for the conditional ablation of microRNAs in the mouse Cell Reports 1(4):385-91. [PubMed: 22570807]  [MGI Ref ID J:181752]

W.M. Keck Center for Noncoding RNAs. 2012. Information obtained from the W.M. Keck Center for Noncoding RNAs Unpublished :.  [MGI Ref ID J:181831]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhile maintaining a live colony, these mice are bred as heterozygotes. Mice homozygous for the mutation are not viable.

Pricing and Purchasing

Supply Notes


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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