Strain Name:

STOCK Mir301tm1Mtm/Mmjax


Cryopreserved - Ready for recovery     Available at the JAX MMRRC

Please refer to the Mutant Mouse Regional Resource Center (MMRRC) for information about STOCK Mir301tm1Mtm/Mmjax MMRRC Stock Number 034652.
These microRNA 301 conditional mutant mice are designed to generate a null allele or a lacZ tagged null allele when combined with Flp or Cre recombinase expressing strains. LacZ expression is widespread. This mutant mouse strain may be useful in studies of microRNA biology.


The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
Generation Definitions
Donating Investigator Mike McManus,   University of California, San Francisco

Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous mice are embryonic lethal. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR301 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR301 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). LacZ expression is widespread. This mutant mouse strain may be useful in studies of microRNA biology.

A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the miR301 stem loop, with one loxP site placed immediately downstream of the miR301 stem loop. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Chimeric mice were crossed with a mouse of mixed genetic background (which incorporated FVB), then crossed to inbred C57BL/6 for two generations. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Control Information

   Wild-type from the colony (approximate)
  Considerations for Choosing Controls


Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.


        involves: 129P2/OlaHsd * C57BL/6
  • no phenotypic analysis
  • *normal* no phenotypic analysis   (MGI Ref ID J:181752)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
      loxP-flanked microRNA sequence

Neurobiology Research
lacZ expression in neural tissue
Cre-lox System
      loxP-flanked Sequences: Test/Reporter

Research Tools
Cre-lox System
      loxP-flanked Sequences: Test/Reporter
FLP-FRT System
      FRT-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol Mir301tm1Mtm
Allele Name targeted mutation 1, Michael McManus
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Strain of Origin129P2/OlaHsd
Site of ExpressionUbiquitous at E11.5
Gene Symbol and Name Mir301, microRNA 301
Chromosome 11
Gene Common Name(s) Mirn301; mir 301; mmu-mir-301; mmu-mir-301a;
Molecular Note A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA stem loop and one loxP site immediately downstream of the microRNA stem loop. [MGI Ref ID J:181752]


Genotyping Information

Genotyping Protocols

Mir301tm1Mtm, Standard PCR

Helpful Links

Genotyping resources and troubleshooting


References provided by MGI

Additional References

Mir301tm1Mtm related

Park CY; Jeker LT; Carver-Moore K; Oh A; Liu HJ; Cameron R; Richards H; Li Z; Adler D; Yoshinaga Y; Martinez M; Nefadov M; Abbas AK; Weiss A; Lanier LL; de Jong PJ; Bluestone JA; Srivastava D; McManus MT. 2012. A resource for the conditional ablation of microRNAs in the mouse Cell Rep 1(4):385-91. [PubMed: 22570807]  [MGI Ref ID J:181752]

W.M. Keck Center for Noncoding RNAs. 2012. Information obtained from the W.M. Keck Center for Noncoding RNAs Unpublished :.  [MGI Ref ID J:181831]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhile maintaining a live colony, these mice are bred as heterozygotes. Mice homozygous for the mutation are not viable.

Pricing and Purchasing

Supply Notes

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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