Strain Name:

STOCK Sox2tm1.1Lan/J

Stock Number:

013093

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
These Sox2flox mutant mice possess loxP sites flanking the coding exon of SRY-box containing gene 2 (Sox2). This strain may be useful for studying eye and lens development.

Description

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Mating SystemHomozygote x Homozygote         (Female x Male)   04-JUN-11
Specieslaboratory mouse
GenerationF?+F1N1F6 (06-MAR-13)
Generation Definitions
 
Donating Investigator Richard A. Lang,   Cincinnati Children's Hospital

Description
These Sox2flox mutant mice possess loxP sites flanking the coding exon of SRY-box containing gene 2 (Sox2). Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express tissue-specific Cre recombinase, the resulting offspring will have no Sox2 expression in cre-expressing tissues. This strain may be useful for studying eye and lens development.

Development
A targeting vector was designed to insert a loxP site upstream of the coding exon of the SRY-box containing gene 2 (Sox2) locus, followed by an frt-flanked neomycin resistance (neo) cassette, and a second loxP site downstream of the exon. The construct was electroporated into 129S/SvEv-derived CMTI-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with B6.SJL-Tg(ACTFLPe)9205Dym/J (Stock No. 003800) mice to delete the neo cassette. Progeny were crossed to remove the FLPe transgene, resulting in a colony homozygous for the floxed-Sox2 (Sox2flox) allele. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Control Information

  Control
   101043 B6129SF1/J (approximate)
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Sox2     (9 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Microphthalmia, Syndromic 3; MCOPS3   (SOX2)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Eye Defects

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Sox2tm1.1Lan
Allele Name targeted mutation 1.1, Richard A Lang
Allele Type Targeted (Floxed/Frt)
Common Name(s) Sox2loxP; Sox2tm1.1Apdn;
Mutation Made By Richard Lang,   Cincinnati Children's Hospital
Gene Symbol and Name Sox2, SRY (sex determining region Y)-box 2
Chromosome 3
Gene Common Name(s) ANOP3; MCOPS3; RGD1565646; Sox-2; lcc; light coat and circling; yellow submarine; ysb;
Molecular Note A loxP site was inserted usptream of the coding exon and an frt flanked neo cassette with a 3' loxP site was inserted downstream of the exon. The frt flanked neo cassette used for selection was removed by germ line, flp mediated recombination. [MGI Ref ID J:152850]

Genotyping

Genotyping Information

Genotyping Protocols

Sox2tm1.1Lan, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Shaham O; Smith AN; Robinson ML; Taketo MM; Lang RA; Ashery-Padan R. 2009. Pax6 is essential for lens fiber cell differentiation. Development 136(15):2567-78. [PubMed: 19570848]  [MGI Ref ID J:152850]

Additional References

Sox2tm1.1Lan related

Hoffmann SA; Hos D; Kuspert M; Lang RA; Lovell-Badge R; Wegner M; Reiprich S. 2014. Stem cell factor Sox2 and its close relative Sox3 have differentiation functions in oligodendrocytes. Development 141(1):39-50. [PubMed: 24257626]  [MGI Ref ID J:206315]

Juuri E; Jussila M; Seidel K; Holmes S; Wu P; Richman J; Heikinheimo K; Chuong CM; Arnold K; Hochedlinger K; Klein O; Michon F; Thesleff I. 2013. Sox2 marks epithelial competence to generate teeth in mammals and reptiles. Development 140(7):1424-32. [PubMed: 23462476]  [MGI Ref ID J:194917]

Smith AN; Miller LA; Radice G; Ashery-Padan R; Lang RA. 2009. Stage-dependent modes of Pax6-Sox2 epistasis regulate lens development and eye morphogenesis. Development 136(17):2977-85. [PubMed: 19666824]  [MGI Ref ID J:158133]

Tompkins DH; Besnard V; Lange AW; Wert SE; Keiser AR; Smith AN; Lang R; Whitsett JA. 2009. Sox2 is required for maintenance and differentiation of bronchiolar Clara, ciliated, and goblet cells. PLoS One 4(12):e8248. [PubMed: 20011520]  [MGI Ref ID J:155963]

Wang S; Xia P; Ye B; Huang G; Liu J; Fan Z. 2013. Transient activation of autophagy via Sox2-mediated suppression of mTOR is an important early step in reprogramming to pluripotency. Cell Stem Cell 13(5):617-25. [PubMed: 24209762]  [MGI Ref ID J:205532]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB27

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.
Mating SystemHomozygote x Homozygote         (Female x Male)   04-JUN-11

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHomozygous for Sox2tm1.1Lan  
Price per Pair (US dollars $)Pair Genotype
$464.00Homozygous for Sox2tm1.1Lan x Homozygous for Sox2tm1.1Lan  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHomozygous for Sox2tm1.1Lan  
Price per Pair (US dollars $)Pair Genotype
$603.20Homozygous for Sox2tm1.1Lan x Homozygous for Sox2tm1.1Lan  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   101043 B6129SF1/J (approximate)
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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