|Please refer to the Mutant Mouse Regional Resource Center (MMRRC) for information about STOCK Gata4tm2(Gata4)Wtp/Mmjax MMRRC Stock Number 032883.|
|These knockin mutant mice possess a floxed GATA4 cDNA with a transcriptional stop cassette followed by an alkaline phosphatase cDNA; cre-mediated excision activates AP expression. This mutant mouse strain may be useful in studies of cell lineage tracing in the developing and adult heart.|
Type Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?pN1
Donating Investigator William T Pu, Children's Hospital Boston, Harvard MS
Homozygotes: Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice show no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Cre-mediated excision of Gata4 is coupled with alkaline phosphatase expression under the control of the endogenous Gata4 regulatory elements. This mutant mouse strain may be useful in studies of cell lineage tracing in the developing and adult heart.
A targeting vector containing a loxP-flanked Gata4 cDNA with a transcriptional stop cassette followed by an alkaline phosphatase cDNA and an FRT-flanked PGKneo cassette was inserted into the 5' UTR of the targeted gene. The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Chimeric animals were crossed to C57BL/6 and SJL mixed background Tg(ACTFLPe)9205Dym mice to remove the neomycin cassette. To remove the FLP transgene, mice were mated with outbred Black Swiss mice and then intercrossed to establish a homozygous colony. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
|Wild-type from the colony||(approximate)|
|Considerations for Choosing Controls|
Strains carrying other alleles of Gata4
006807 B6Ei.129S6(Cg)-Gata4tm1.1Sho/EiJ 006808 D2.129S6(Cg)-Gata4tm1.1Sho/EiJ 017395 FVB/N-Gata4Tg(Tyr)2265AOve/Mmjax 017544 STOCK Gata4tm1.1Jmol/J 008194 STOCK Gata4tm1.1Sad/J 018121 STOCK Gt(ROSA)26Sortm1(birA)Mejr Gata4tm3.1Wtp/J 016572 STOCK Tg(Myh6/tetO-Gata4)1Jmol/JView Strains carrying other alleles of Gata4 (7 strains)
View Related Disease (OMIM) TermsRelated Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.View Mammalian Phenotype TermsMammalian Phenotype Terms provided by MGIassigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services firstname.lastname@example.org for customized breeding options.
Gata4tm2(Gata4)Wtp/Gata4+ Tg(Myh6-cre)2182Mds/0involves: 129S6/SvEvTac * FVB/N (conditional)
- no phenotypic analysis
- *normal* no phenotypic analysis
- used for cell lineage tracing (MGI Ref ID J:137426)View Research ApplicationsResearch ApplicationsThis mouse can be used to support research in many areas including:
loxP-flanked Sequences: Test/Reporter
|Allele Name||targeted mutation 2, William T Pu|
|Mutation Made By||William Pu, Children's Hospital Boston, Harvard MS|
|Strain of Origin||129S6/SvEvTac|
|Expressed Gene||Gata4, GATA binding protein 4, mouse, laboratory|
|Molecular Note||Exon 2 was replaced with a floxed full-length Gata4 cDNA with a transcription STOP sequence followed by an alkaline phosphatase gene with a 3' frt site. An frt-flanked neo cassette used for selection purposes was removed by germ line, flp-mediated recombination. Cre mediated recombination is necessary to activate alkaline phospatase expression. [MGI Ref ID J:137426]|
Zhou B; Ma Q; Rajagopal S; Wu SM; Domian I; Rivera-Feliciano J; Jiang D; von Gise A; Ikeda S; Chien KR; Pu WT. 2008. Epicardial progenitors contribute to the cardiomyocyte lineage in the developing heart. Nature 454(7200):109-13. [PubMed: 18568026] [MGI Ref ID J:137426]
Aronson BE; Rabello Aronson S; Berkhout RP; Chavoushi SF; He A; Pu WT; Verzi MP; Krasinski SD. 2014. GATA4 represses an ileal program of gene expression in the proximal small intestine by inhibiting the acetylation of histone H3, lysine 27. Biochim Biophys Acta 1839(11):1273-82. [PubMed: 24878542] [MGI Ref ID J:217928]
Manuylov NL; Zhou B; Ma Q; Fox SC; Pu WT; Tevosian SG. 2011. Conditional ablation of Gata4 and Fog2 genes in mice reveals their distinct roles in mammalian sexual differentiation. Dev Biol 353(2):229-41. [PubMed: 21385577] [MGI Ref ID J:173838]
Animal Health ReportsProduction of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Breeding & Husbandry While maintaining a live colony, these mice are bred as homozygotes.
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