Strain Name:

STOCK Gata4tm2(Gata4)Wtp/Mmjax

Availability:

Cryopreserved - Ready for recovery     Available at the JAX MMRRC

Please refer to the Mutant Mouse Regional Resource Center (MMRRC) for information about STOCK Gata4tm2(Gata4)Wtp/Mmjax MMRRC Stock Number 032883.
These knockin mutant mice possess a floxed GATA4 cDNA with a transcriptional stop cassette followed by an alkaline phosphatase cDNA; cre-mediated excision activates AP expression. This mutant mouse strain may be useful in studies of cell lineage tracing in the developing and adult heart.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
Generation?pN1
Generation Definitions
 
Donating Investigator William T Pu,   Children's Hospital Boston, Harvard MS

Description
Homozygotes: Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice show no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Cre-mediated excision of Gata4 is coupled with alkaline phosphatase expression under the control of the endogenous Gata4 regulatory elements. This mutant mouse strain may be useful in studies of cell lineage tracing in the developing and adult heart.

Heterozygote: Normal

Development
A targeting vector containing a loxP-flanked Gata4 cDNA with a transcriptional stop cassette followed by an alkaline phosphatase cDNA and an FRT-flanked PGKneo cassette was inserted into the 5' UTR of the targeted gene. The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Chimeric animals were crossed to C57BL/6 and SJL mixed background Tg(ACTFLPe)9205Dym mice to remove the neomycin cassette. To remove the FLP transgene, mice were mated with outbred Black Swiss mice and then intercrossed to establish a homozygous colony. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Control Information

  Control
   Wild-type from the colony (approximate)
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Gata4     (7 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Atrial Septal Defect 2; ASD2   (GATA4)
Atrioventricular Septal Defect 4; AVSD4   (GATA4)
Testicular Anomalies with or without Congenital Heart Disease; TACHD   (GATA4)
Ventricular Septal Defect 1; VSD1   (GATA4)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
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Gata4tm2(Gata4)Wtp/Gata4+ Tg(Myh6-cre)2182Mds/0

        involves: 129S6/SvEvTac * FVB/N   (conditional)
  • no phenotypic analysis
  • *normal* no phenotypic analysis
    • used for cell lineage tracing   (MGI Ref ID J:137426)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cardiovascular Research
      Cre-lox System
Cre-lox System
      loxP-flanked Sequences: Test/Reporter

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Gata4tm2(Gata4)Wtp
Allele Name targeted mutation 2, William T Pu
Allele Type Targeted
Common Name(s) Gata4flap;
Mutation Made By William Pu,   Children's Hospital Boston, Harvard MS
Strain of Origin129S6/SvEvTac
Expressed Gene Gata4, GATA binding protein 4, mouse, laboratory
Molecular Note Exon 2 was replaced with a floxed full-length Gata4 cDNA with a transcription STOP sequence followed by an alkaline phosphatase gene with a 3' frt site. An frt-flanked neo cassette used for selection purposes was removed by germ line, flp-mediated recombination. Cre mediated recombination is necessary to activate alkaline phospatase expression. [MGI Ref ID J:137426]

Genotyping

Genotyping Information

Genotyping Protocols

Gata4tm2(Gata4)Wtp, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Ma Q; Zhou B; Pu WT. 2008. Reassessment of Isl1 and Nkx2-5 cardiac fate maps using a Gata4-based reporter of Cre activity. Dev Biol 323(1):98-104. [PubMed: 18775691]  [MGI Ref ID J:141152]

Zhou B; Ma Q; Rajagopal S; Wu SM; Domian I; Rivera-Feliciano J; Jiang D; von Gise A; Ikeda S; Chien KR; Pu WT. 2008. Epicardial progenitors contribute to the cardiomyocyte lineage in the developing heart. Nature 454(7200):109-13. [PubMed: 18568026]  [MGI Ref ID J:137426]

Additional References

Gata4tm2(Gata4)Wtp related

Manuylov NL; Zhou B; Ma Q; Fox SC; Pu WT; Tevosian SG. 2011. Conditional ablation of Gata4 and Fog2 genes in mice reveals their distinct roles in mammalian sexual differentiation. Dev Biol 353(2):229-41. [PubMed: 21385577]  [MGI Ref ID J:173838]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhile maintaining a live colony, these mice are bred as homozygotes.

Pricing and Purchasing

Supply Notes


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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