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| These Grn floxed mutant mice possess a single loxP site upstream of the promoter region of the granulin (Grn) gene, and a second loxP site fused to a FRT-flanked neomycin/kanamycin (neo/kan) resistance cassette downstream of exon 4. This floxed strain may be used to generate whole mouse or tissue-specific mutants that are useful for studying inflammation, infectious disease, frontotemporal dementia and other neurodegenerative diseases. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Coisogenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Aihao Ding, Weill Medical School of Cornell Universi Description
These Grn floxed mutant mice possess a single loxP site upstream of the promoter region of the granulin (Grn) gene, and a second loxP site fused to a FRT-flanked neomycin/kanamycin (neo/kan) resistance cassette downstream of exon 4. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 1- 4 deleted in cre-expressing tissues. When bred with mice expressing Cre-recombinase driven by the chicken actin gene promoter (CAG-cre), granulin deficiency is widespread (see Stock No. 013175). These mice exhibit neurodegeneration as seen in human frontotemporal dementia and have an exaggerated inflammation response when exposed to microbial agents. This floxed strain may be used to generate whole mouse or tissue-specific mutants that are useful for studying inflammation, infectious disease, frontotemporal dementia and other neurodegenerative diseases.Development
A targeting vector was designed to insert a single loxP site upstream of the promoter region, and a second loxP site 3' of a FRT-flanked neomycin/kanamycin (neo/kan) resistance cassette were placed downstream of exon 4 of the granulin (Grn) gene. The construct was electroporated into c2J embryonic stem (ES) cells (derived from albino C57BL/6J-Tyrc-2j mice). Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with albino C57BL/6J-Tyrc-2j mice. Resulting offspring were bred together to establish a colony of Grnflox/flox mice. Upon arrival at The Jackson Laboratory, Grnflox/flox mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony. These mutant mice may still be segregating for the Tyrc-2J mutation.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Grn
013175 B6(Cg)-Grntm1.1Aidi/J View Strains carrying other alleles of Grn (1 strain)
Additional Web Information
Use of MICE by companies or for-profit entities requires a license prior to shipping.
Use of MICE by companies or for-profit entities requires a license.
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Ceroid Lipofuscinosis, Neuronal, 11; CLN11 (GRN)
Frontotemporal Lobar Degeneration with Tdp43 Inclusions, Grn-Related (GRN)
assigned by genotype
Grntm1Aidi/Grn+
involves: C57BL/6 * C57BL/6J
- no phenotypic analysis
- *normal* no phenotypic analysis
- mice were used to generate Grntm1.1Aidi mice (MGI Ref ID J:156824)
This mouse can be used to support research in many areas including:
Neurobiology Research
Neurodegeneration
Neurodevelopmental Defects
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Grntm1Aidi | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Aihao Ding | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | pgrnflox; | ||
| Mutation Made By | Aihao Ding, Weill Medical School of Cornell Universi | ||
| Strain of Origin | C57BL/6 | ||
| Gene Symbol and Name | Grn, granulin | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | CLN11; GEP; GP88; PC cell-derived growth factor; PCDGF; PEPI; PGRN; acrogranulin; epithelin; progranulin; | ||
| General Note | ES cell line = C2J (C57BL/6) | ||
| Molecular Note | A loxP site was inserted upstream of exon 1 and an frt floxed neo cassette with a 3' loxP site was inserted downstream of exon 4. [MGI Ref ID J:156824] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Grntm1Aidi, Standard PCR
Tyrc-2J alternative 1, End Point Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Yin F; Banerjee R; Thomas B; Zhou P; Qian L; Jia T; Ma X; Ma Y; Iadecola C; Beal MF; Nathan C; Ding A. 2010. Exaggerated inflammation, impaired host defense, and neuropathology in progranulin-deficient mice. J Exp Med 207(1):117-28, S1-4. [PubMed: 20026663] [MGI Ref ID J:156824]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygotes may be bred together.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.