|Please refer to the Mutant Mouse Regional Resource Center (MMRRC) for information about STOCK Mir30etm1Mtm/Mmjax MMRRC Stock Number 034656.|
|These microRNA 30e conditional mutant mice are designed to generate a null allele or a lacZ tagged null allele when combined with Flp or Cre recombinase expressing strains. This mutant mouse strain may be useful in studies of microRNA biology.|
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?pN1
Donating Investigator Mike McManus, University of California, San Francisco
Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR30e stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR30e stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). Homozygous mice have not been characterized. This mutant mouse strain may be useful in studies of microRNA biology.
A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the miR30e stem loop, with one loxP site placed immediately downstream of the miR30e stem loop. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Chimeric mice were crossed with a mouse of mixed genetic background (which incorporated FVB), then crossed to inbred C57BL/6 for one generation. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
|Wild-type from the colony||(approximate)|
|Considerations for Choosing Controls|
View Mammalian Phenotype TermsMammalian Phenotype Terms provided by MGIassigned by genotype
The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.
Mir30etm1Mtm/Mir30etm1Mtminvolves: 129P2/OlaHsd * C57BL/6
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:181752)
View Research ApplicationsResearch ApplicationsThis mouse can be used to support research in many areas including:
Cell Biology Research
loxP-flanked microRNA sequence
lacZ expression in neural tissue
loxP-flanked Sequences: Test/Reporter
loxP-flanked Sequences: Test/Reporter
|Allele Name||targeted mutation 1, Michael McManus|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Strain of Origin||129P2/OlaHsd|
|Gene Symbol and Name||Mir30e, microRNA 30e|
|Gene Common Name(s)||Mirn30e; mir 30e; mmu-mir-30e;|
|Molecular Note||A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA stem loop and one loxP site immediately downstream of the microRNA stem loop. [MGI Ref ID J:181752]|
Park CY; Jeker LT; Carver-Moore K; Oh A; Liu HJ; Cameron R; Richards H; Li Z; Adler D; Yoshinaga Y; Martinez M; Nefadov M; Abbas AK; Weiss A; Lanier LL; de Jong PJ; Bluestone JA; Srivastava D; McManus MT. 2012. A resource for the conditional ablation of microRNAs in the mouse Cell Rep 1(4):385-91. [PubMed: 22570807] [MGI Ref ID J:181752]
W.M. Keck Center for Noncoding RNAs. 2012. Information obtained from the W.M. Keck Center for Noncoding RNAs Unpublished :. [MGI Ref ID J:181831]
Animal Health ReportsProduction of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Breeding & Husbandry While maintaining a live colony, these mice have been bred as heterozygotes. The Donating Investigator has not developed a homozygous colony.
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