Strain Name:

B6.Cg-Rptortm1.1Dmsa/J

Stock Number:

013188

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
These floxed mutant mice possess loxP sites flanking exon 6 of the targeted Rptor (regulatory associated protein of MTOR, complex 1) gene. This strain may be useful for studying mTOR-dependent regulation of ketogenesis and other cellular processes in response to feeding and fasting.

Description

Strain Information

Former Names B6.Cg-Rptortm2.1Lex/J    (Changed: 05-OCT-11 )
Type Congenic; Mutant Strain; Targeted Mutation;
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Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   11-DEC-12
Specieslaboratory mouse
GenerationN5+N1F5 (11-DEC-13)
Generation Definitions
 
Donating Investigator David Sabatini,   Whitehead Institute

Description
These floxed mutant mice possess loxP sites flanking exon 6 of the targeted Rptor (regulatory associated protein of MTOR, complex 1) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to C57BL6-Tg(AlbCre)21Mgn (Stock No. 003574) mice, which direct liver-specific expression of Cre, mice exhibit a decrease in liver size yet produce ketones for hours after feeding. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 6 deleted in the cre-expressing tissues. This strain may be useful for studying mTOR (mammalian or mechanistic target of rapamycin)-dependent regulation of ketogenesis and other cellular processes in response to feeding and fasting.

Development
A targeting vector was designed to insert a loxP site upstream of exon 6 followed by a frt-flanked neomycin (neo) resistance cassette, followed by loxP sites flanking exon 6 of the regulatory associated protein of MTOR (Mechanistic target of rapamycin), complex 1 (Rptor) gene. The construct was electroporated into 129/SvEv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c blastocysts and resulting chimeric mice were bred to C57BL/6 mice. Heterozygous offspring were bred with Flp transgenic mice on a 129 background to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene, resulting in a colony homozygous for the floxed-Rptor (Rptorflox/flox) allele. The donating investigator reports that these mice were backcrossed to C57BL/6 (see SNP note below) at least 4 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 1 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Rptor
013191   B6.129S5-Rptortm1Lex/J
View Strains carrying other alleles of Rptor     (1 strain)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Rptortm1.1Dmsa/Rptortm1.1Dmsa Tg(Alb-cre)21Mgn/0

        involves: 129S/SvEv * C57BL/6 * DBA   (conditional)
  • liver/biliary system phenotype
  • abnormal hepatocyte morphology
    • decrease in hepatocyte size   (MGI Ref ID J:167208)
  • abnormal liver physiology
    • decrease in liver protein content   (MGI Ref ID J:167208)
    • fasting does not decrease the size of the liver, unlike in controls   (MGI Ref ID J:167208)
  • small liver
    • about a 40% decrease in liver mass compared to controls   (MGI Ref ID J:167208)
  • homeostasis/metabolism phenotype
  • increased circulating ketone body level
    • at early times after refeeding compared to wild-type controls   (MGI Ref ID J:167208)
    • feeding mice sodium octanoate after a fast results in prolonged elevation of ketone levels   (MGI Ref ID J:167208)
    • aged mice do not display a reduction in ketone production during fasting unlike in aged wild-type controls   (MGI Ref ID J:167208)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Internal/Organ Defects
      liver

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Rptortm1.1Dmsa
Allele Name targeted mutation 1.1, David M Sabatini
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) RaploxP;
Strain of Origin129S/SvEv
Gene Symbol and Name Rptor, regulatory associated protein of MTOR, complex 1
Chromosome 11
Gene Common Name(s) 4932417H02Rik; KOG1; Mip1; RGD1311784; RIKEN cDNA 4932417H02 gene; Rap; mKIAA1303; raptor;
Molecular Note Exon 6 was flanked by 5' and 3' LoxP sites located 111 bp upstream and 547 bp downstream, respectively, and an FRT flanked neo cassette was inserted via homologous recombination. The neo cassette was removed by flp mediated recombination. [MGI Ref ID J:167208]

Genotyping

Genotyping Information

Genotyping Protocols

Rptortm2.1Lex, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Rptortm1.1Dmsa related

Bercury KK; Dai J; Sachs HH; Ahrendsen JT; Wood TL; Macklin WB. 2014. Conditional ablation of raptor or rictor has differential impact on oligodendrocyte differentiation and CNS myelination. J Neurosci 34(13):4466-80. [PubMed: 24671993]  [MGI Ref ID J:210456]

Chen J; Long F. 2014. mTORC1 signaling controls mammalian skeletal growth through stimulation of protein synthesis. Development 141(14):2848-54. [PubMed: 24948603]  [MGI Ref ID J:213870]

Everts B; Amiel E; Huang SC; Smith AM; Chang CH; Lam WY; Redmann V; Freitas TC; Blagih J; van der Windt GJ; Artyomov MN; Jones RG; Pearce EL; Pearce EJ. 2014. TLR-driven early glycolytic reprogramming via the kinases TBK1-IKKvarepsilon supports the anabolic demands of dendritic cell activation. Nat Immunol 15(4):323-32. [PubMed: 24562310]  [MGI Ref ID J:209894]

Hung CM; Calejman CM; Sanchez-Gurmaches J; Li H; Clish CB; Hettmer S; Wagers AJ; Guertin DA. 2014. Rictor/mTORC2 Loss in the Myf5 Lineage Reprograms Brown Fat Metabolism and Protects Mice against Obesity and Metabolic Disease. Cell Rep 8(1):256-71. [PubMed: 25001283]  [MGI Ref ID J:212069]

Kalaitzidis D; Sykes SM; Wang Z; Punt N; Tang Y; Ragu C; Sinha AU; Lane SW; Souza AL; Clish CB; Anastasiou D; Gilliland DG; Scadden DT; Guertin DA; Armstrong SA. 2012. mTOR complex 1 plays critical roles in hematopoiesis and Pten-loss-evoked leukemogenesis. Cell Stem Cell 11(3):429-39. [PubMed: 22958934]  [MGI Ref ID J:193428]

Peterson TR; Sengupta SS; Harris TE; Carmack AE; Kang SA; Balderas E; Guertin DA; Madden KL; Carpenter AE; Finck BN; Sabatini DM. 2011. mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. Cell 146(3):408-20. [PubMed: 21816276]  [MGI Ref ID J:174681]

Rodgers JT; King KY; Brett JO; Cromie MJ; Charville GW; Maguire KK; Brunson C; Mastey N; Liu L; Tsai CR; Goodell MA; Rando TA. 2014. mTORC1 controls the adaptive transition of quiescent stem cells from G0 to G(Alert). Nature 510(7505):393-6. [PubMed: 24870234]  [MGI Ref ID J:213273]

Sengupta S; Peterson TR; Laplante M; Oh S; Sabatini DM. 2010. mTORC1 controls fasting-induced ketogenesis and its modulation by ageing. Nature 468(7327):1100-4. [PubMed: 21179166]  [MGI Ref ID J:167208]

Shin J; Wang S; Deng W; Wu J; Gao J; Zhong XP. 2014. Mechanistic target of rapamycin complex 1 is critical for invariant natural killer T-cell development and effector function. Proc Natl Acad Sci U S A 111(8):E776-83. [PubMed: 24516149]  [MGI Ref ID J:206820]

Wan M; Leavens KF; Saleh D; Easton RM; Guertin DA; Peterson TR; Kaestner KH; Sabatini DM; Birnbaum MJ. 2011. Postprandial hepatic lipid metabolism requires signaling through Akt2 independent of the transcription factors FoxA2, FoxO1, and SREBP1c. Cell Metab 14(4):516-27. [PubMed: 21982711]  [MGI Ref ID J:177644]

Yang K; Shrestha S; Zeng H; Karmaus PW; Neale G; Vogel P; Guertin DA; Lamb RF; Chi H. 2013. T cell exit from quiescence and differentiation into Th2 cells depend on Raptor-mTORC1-mediated metabolic reprogramming. Immunity 39(6):1043-56. [PubMed: 24315998]  [MGI Ref ID J:209300]

Zeng H; Yang K; Cloer C; Neale G; Vogel P; Chi H. 2013. mTORC1 couples immune signals and metabolic programming to establish T(reg)-cell function. Nature 499(7459):485-90. [PubMed: 23812589]  [MGI Ref ID J:204754]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.
Mating SystemHomozygote x Homozygote         (Female x Male)   11-DEC-12

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $239.00Female or MaleHomozygous for Rptortm1.1Lex  
Price per Pair (US dollars $)Pair Genotype
$478.00Homozygous for Rptortm1.1Lex x Homozygous for Rptortm1.1Lex  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $310.70Female or MaleHomozygous for Rptortm1.1Lex  
Price per Pair (US dollars $)Pair Genotype
$621.40Homozygous for Rptortm1.1Lex x Homozygous for Rptortm1.1Lex  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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