Strain Name:

B6.Cg-Ptprca Mir223tm1Fcam/J

Stock Number:

013198

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
In this strain, the allele replaces the entire coding region of the microRNA-223 (Mir223) gene with a frt-flanked neomycin (neo) resistance cassette, abolishing gene function. These mice also harbor the CD45.1 (Ly5.1 or Ptprca) allele rather than the CD45.2 (Ly5.2 or Ptprcb) allele normally present in C57BL/6 mice. These mice may be useful for studying granulocyte production and inflammatory response.

Description

Strain Information

Former Names B6.Cg-Ptprca Mir223Fcam/J    (Changed: 19-AUG-10 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemSee Colony Maintenance under the Health & Care tab         (Female x Male)   31-OCT-11
Specieslaboratory mouse
GenerationN6+F5 (24-NOV-13)
Generation Definitions
 
Donating Investigator David Bartel,   MIT Division of Comparative Medicine

Description
In this strain, the allele replaces the entire coding region of the microRNA-223 (Mir223) gene with a frt-flanked neomycin (neo) resistance cassette, abolishing gene function. They also harbor the CD45.1 (Ly5.1 or Ptprca) allele rather than the CD45.2 (Ly5.2 or Ptprcb) allele normally present in C57BL/6 mice. Homozygous miR-223- mice are viable, fertile, and normal in size. After endotoxin challenge, these mice exhibit an increase in aspartate aminotransferase (ALT), blood urea nitrogen (BUN) and creatine kinase (CK) levels due to widespread tissue destruction, including hepatocyte necrosis and intralobular haemorrhage. They also develop inflammatory lung pathology characterized by areas of atelectasis, increased cellularity within the parenchyma, and inflammatory infiltration into the interstitium. Granulocytes from miR-223- mice are hypermature, hypersensitive to activating stimuli, and display increased fungicidal activity. These mice have an expanded granulocytic compartment resulting from a cellautonomous increase in the number of granulocyte progenitors. These mice may be useful for studying granulocyte production and inflammatory response.

Development
A targeting vector was designed to replace the entire coding region of the microRNA-223 (Mir223) gene with a frt-flanked neomycin (neo) resistance cassette. The construct was electroporated into (C57BL/6 x 129S4Sv/Jae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6 females to generate a colony of miR-223- mice. The donating investigator reported that these mice were backcrossed for at least 5 generations to B6.SJL-Ptprca Pepcb/BoyJ (Stock No. 002014) and thus also harbor the CD45.1 (Ly5.1 or Ptprca) allele (see SNP note below). These mice may still be segregating at the Pepcb allele. Upon arrival at The Jackson Laboratory, mice were bred to B6.SJL-Ptprca Pepcb/BoyJ for at least one generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. At least 1 marker, on Chromosome 11, is segregating. Also,4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Control Information

  Control
   002014 B6.SJL-Ptprca Pepcb/BoyJ (approximate)
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying   Ptprca     (9 strains)

View Strains carrying other alleles of Ptprc     (10 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Hepatitis C Virus, Susceptibility to   (PTPRC)
Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-Negative, B Cell-Positive, Nk Cell-Positive   (PTPRC)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Mir223tm1Fcam/Mir223tm1Fcam

        involves: 129S4/SvJae * C57BL/6
  • immune system phenotype
  • abnormal granulocyte physiology
    • granulocytes have higher rates of proliferation with approximately 2-fold more granulocytes actively proliferating in the bone marrow than in controls   (MGI Ref ID J:140009)
    • mutant bone marrow cells generate 2.3-fold more colonies than wild-type bone marrow in methylcellulose cultures with granulocyte colony stimulating factor   (MGI Ref ID J:140009)
    • competitive bone marrow chimera experiments confirm that granulocytes develop into neutrophils at a faster rate   (MGI Ref ID J:140009)
    • abnormal neutrophil physiology
      • neutrophils are hypersensitive to activating stimuli   (MGI Ref ID J:140009)
      • neutrophils have a 50% higher oxidative burst than controls upon low-dose PMA stimulation   (MGI Ref ID J:140009)
      • neutrophils display 25% higher killing when co-cultured with Candida albicans   (MGI Ref ID J:140009)
  • abnormal neutrophil differentiation
    • neutrophils exhibit an unusual hypermature morphology characterized by nuclear hypersegmentation and blebbing   (MGI Ref ID J:140009)
    • neutrophil markers are aberrantly expressed with decreases in Ly-6G and Mac-1 expression in the periphery and increases of 7/4 expression in the bone marrow   (MGI Ref ID J:140009)
  • increased acute inflammation
    • low-dose LPS administration leads to distress caused by endotoxaimia   (MGI Ref ID J:140009)
    • after LPS administration, mice have significantly higher levels of aspartate aminotransferase, blood urea nitrogen and creatine kinase, indicative of considerable widespread tissue damage   (MGI Ref ID J:140009)
    • hepatocyte necrosis and intralobular haemorrhage are also observed after LPS administration   (MGI Ref ID J:140009)
  • increased granulocyte number
    • the number of granulocytes are found in the bone marrow is increased by more than a third   (MGI Ref ID J:140009)
    • increased neutrophil cell number
      • there are higher numbers of neutrophils in the blood with 5.76 x 105 cells per ml found versus 2.5 x 105 in controls   (MGI Ref ID J:140009)
  • lung inflammation
    • mice over 1.2 years of age consistently display inflammatory lung pathology characterized by areas of atelectasis, increased cellularity within the parenchyma, and inflammatory infiltration into the interstitium   (MGI Ref ID J:140009)
    • neutrophils are the most prominent cell type in this infiltrate   (MGI Ref ID J:140009)
  • hematopoietic system phenotype
  • abnormal granulocyte physiology
    • granulocytes have higher rates of proliferation with approximately 2-fold more granulocytes actively proliferating in the bone marrow than in controls   (MGI Ref ID J:140009)
    • mutant bone marrow cells generate 2.3-fold more colonies than wild-type bone marrow in methylcellulose cultures with granulocyte colony stimulating factor   (MGI Ref ID J:140009)
    • competitive bone marrow chimera experiments confirm that granulocytes develop into neutrophils at a faster rate   (MGI Ref ID J:140009)
    • abnormal neutrophil physiology
      • neutrophils are hypersensitive to activating stimuli   (MGI Ref ID J:140009)
      • neutrophils have a 50% higher oxidative burst than controls upon low-dose PMA stimulation   (MGI Ref ID J:140009)
      • neutrophils display 25% higher killing when co-cultured with Candida albicans   (MGI Ref ID J:140009)
  • abnormal neutrophil differentiation
    • neutrophils exhibit an unusual hypermature morphology characterized by nuclear hypersegmentation and blebbing   (MGI Ref ID J:140009)
    • neutrophil markers are aberrantly expressed with decreases in Ly-6G and Mac-1 expression in the periphery and increases of 7/4 expression in the bone marrow   (MGI Ref ID J:140009)
  • increased granulocyte number
    • the number of granulocytes are found in the bone marrow is increased by more than a third   (MGI Ref ID J:140009)
    • increased neutrophil cell number
      • there are higher numbers of neutrophils in the blood with 5.76 x 105 cells per ml found versus 2.5 x 105 in controls   (MGI Ref ID J:140009)
  • respiratory system phenotype
  • lung inflammation
    • mice over 1.2 years of age consistently display inflammatory lung pathology characterized by areas of atelectasis, increased cellularity within the parenchyma, and inflammatory infiltration into the interstitium   (MGI Ref ID J:140009)
    • neutrophils are the most prominent cell type in this infiltrate   (MGI Ref ID J:140009)
  • cellular phenotype
  • abnormal neutrophil differentiation
    • neutrophils exhibit an unusual hypermature morphology characterized by nuclear hypersegmentation and blebbing   (MGI Ref ID J:140009)
    • neutrophil markers are aberrantly expressed with decreases in Ly-6G and Mac-1 expression in the periphery and increases of 7/4 expression in the bone marrow   (MGI Ref ID J:140009)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Hematological Research
Neutrophil Defects

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency
      Neutrophil Defects
Inflammation
      Neutrophil defects

Internal/Organ Research
Lung Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Mir223tm1Fcam
Allele Name targeted mutation 1, Fernando D Camargo
Allele Type Targeted (Null/Knockout)
Common Name(s) miR-223-;
Mutation Made By Fernando Camargo,   MIT Division of Comparative Medicine
Strain of Origin(C57BL/6 x 129S4/SvJae)F1
Gene Symbol and Name Mir223, microRNA 223
Chromosome X
Gene Common Name(s) Mirn223; miR-223; mmu-mir-223;
Molecular Note The entire 110 bp locus was replaced by a neomycin resistance cassette. Northern blot analysis of granulocyte RNA from hemizygous mice revealed a complete absence of locus expression. [MGI Ref ID J:140009]
 
Allele Symbol Ptprca
Allele Name a variant
Allele Type Not Applicable
Common Name(s) CD45.1; Ly5a; PtprcSJL;
Site of ExpressionWidely expressed on all adaptive and innate immune cells.
Gene Symbol and Name Ptprc, protein tyrosine phosphatase, receptor type, C
Chromosome 1
Gene Common Name(s) B220; CD45; CD45 antigen; CD45R; Cd45; GP180; L-CA; LCA; LY5; Ly-5; Lyt-4; RT7; T-lymphocyte antigen 4; T200; lymphocyte antigen 5;
Molecular Note Ptprca is found in strains SJL/J, STS/A, and DA. Ptprcb is found in strains C57BL/6, C3H/An, DBA/2, AKR, and many others (J:13367, J:12054, J:12077, J:8603). Twelve nucleotide differences between the a and b alleles have been identified. These base substitutions correspond to five amino-acid changes within the extracellular domain of the encoded protein. These amino-acid differences are clustered in a region that also contains the greatest divergence between mouse and rat sequences (J:22485).

Note that the allele designations originally described were reversed in 1987 (J:8603); all publications prior to 1987 show SJL/J, STS/A, and DA as having the b allele and the C57BL/6J group as having the a allele (J:22341). [MGI Ref ID J:12548] [MGI Ref ID J:22485] [MGI Ref ID J:8080]

Genotyping

Genotyping Information

Genotyping Protocols

Mir223tm1Fcam, Standard PCR
Ptprcab End Point, End Point Analysis


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Johnnidis JB; Harris MH; Wheeler RT; Stehling-Sun S; Lam MH; Kirak O; Brummelkamp TR; Fleming MD; Camargo FD. 2008. Regulation of progenitor cell proliferation and granulocyte function by microRNA-223. Nature 451(7182):1125-9. [PubMed: 18278031]  [MGI Ref ID J:140009]

Additional References

Mir223tm1Fcam related

Baek D; Villen J; Shin C; Camargo FD; Gygi SP; Bartel DP. 2008. The impact of microRNAs on protein output. Nature 455(7209):64-71. [PubMed: 18668037]  [MGI Ref ID J:139940]

Dorhoi A; Iannaccone M; Farinacci M; Fae KC; Schreiber J; Moura-Alves P; Nouailles G; Mollenkopf HJ; Oberbeck-Muller D; Jorg S; Heinemann E; Hahnke K; Lowe D; Del Nonno F; Goletti D; Capparelli R; Kaufmann SH. 2013. MicroRNA-223 controls susceptibility to tuberculosis by regulating lung neutrophil recruitment. J Clin Invest :. [PubMed: 24084739]  [MGI Ref ID J:203989]

Harraz MM; Eacker SM; Wang X; Dawson TM; Dawson VL. 2012. MicroRNA-223 is neuroprotective by targeting glutamate receptors. Proc Natl Acad Sci U S A 109(46):18962-7. [PubMed: 23112146]  [MGI Ref ID J:191741]

Lu TX; Lim EJ; Besse JA; Itskovich S; Plassard AJ; Fulkerson PC; Aronow BJ; Rothenberg ME. 2013. miR-223 Deficiency Increases Eosinophil Progenitor Proliferation. J Immunol 190(4):1576-82. [PubMed: 23325891]  [MGI Ref ID J:193404]

Wang X; Huang W; Yang Y; Wang Y; Peng T; Chang J; Caldwell CC; Zingarelli B; Fan GC. 2014. Loss of duplexmiR-223 (5p and 3p) aggravates myocardial depression and mortality in polymicrobial sepsis. Biochim Biophys Acta 1842(5):701-11. [PubMed: 24486439]  [MGI Ref ID J:211589]

Zhuang G; Meng C; Guo X; Cheruku PS; Shi L; Xu H; Li H; Wang G; Evans AR; Safe S; Wu C; Zhou B. 2012. A novel regulator of macrophage activation: miR-223 in obesity-associated adipose tissue inflammation. Circulation 125(23):2892-903. [PubMed: 22580331]  [MGI Ref ID J:198609]

Ptprca related

Ashbaugh JJ; Brambilla R; Karmally SA; Cabello C; Malek TR; Bethea JR. 2013. IL7Ralpha Contributes to Experimental Autoimmune Encephalomyelitis through Altered T Cell Responses and Nonhematopoietic Cell Lineages. J Immunol 190(9):4525-34. [PubMed: 23530149]  [MGI Ref ID J:195519]

Ataera H; Simkins HM; Hyde E; Yang J; Hermans IF; Petersen TR; Ronchese F. 2013. The control of CD8+ T cell responses is preserved in perforin-deficient mice and released by depletion of CD4+CD25+ regulatory T cells. J Leukoc Biol 94(4):825-33. [PubMed: 23883515]  [MGI Ref ID J:205311]

Ball EA; Sambo MR; Martins M; Trovoada MJ; Benchimol C; Costa J; Antunes Goncalves L; Coutinho A; Penha-Goncalves C. 2013. IFNAR1 controls progression to cerebral malaria in children and CD8+ T cell brain pathology in Plasmodium berghei-infected mice. J Immunol 190(10):5118-27. [PubMed: 23585679]  [MGI Ref ID J:202560]

Basu S; Ray A; Dittel BN. 2011. Cannabinoid receptor 2 is critical for the homing and retention of marginal zone B lineage cells and for efficient T-independent immune responses. J Immunol 187(11):5720-32. [PubMed: 22048769]  [MGI Ref ID J:179699]

Belyaev NN; Biro J; Langhorne J; Potocnik AJ. 2013. Extramedullary myelopoiesis in malaria depends on mobilization of myeloid-restricted progenitors by IFN-gamma induced chemokines. PLoS Pathog 9(6):e1003406. [PubMed: 23762028]  [MGI Ref ID J:213395]

Boettcher S; Gerosa RC; Radpour R; Bauer J; Ampenberger F; Heikenwalder M; Kopf M; Manz MG. 2014. Endothelial cells translate pathogen signals into G-CSF-driven emergency granulopoiesis. Blood 124(9):1393-403. [PubMed: 24990886]  [MGI Ref ID J:214513]

Buscarlet M; Krasteva V; Ho L; Simon C; Hebert J; Wilhelm B; Crabtree GR; Sauvageau G; Thibault P; Lessard JA. 2014. Essential role of BRG, the ATPase subunit of BAF chromatin remodeling complexes, in leukemia maintenance. Blood 123(11):1720-8. [PubMed: 24478402]  [MGI Ref ID J:210092]

Cho RH; Sieburg HB; Muller-Sieburg CE. 2008. A new mechanism for the aging of hematopoietic stem cells: aging changes the clonal composition of the stem cell compartment but not individual stem cells. Blood 111(12):5553-61. [PubMed: 18413859]  [MGI Ref ID J:162992]

Coleman MA; Bridge JA; Lane SW; Dixon CM; Hill GR; Wells JW; Thomas R; Steptoe RJ. 2013. Tolerance induction with gene-modified stem cells and immune-preserving conditioning in primed mice: restricting antigen to differentiated antigen-presenting cells permits efficacy. Blood 121(6):1049-58. [PubMed: 23233664]  [MGI Ref ID J:194614]

De Santo C; Arscott R; Booth S; Karydis I; Jones M; Asher R; Salio M; Middleton M; Cerundolo V. 2010. Invariant NKT cells modulate the suppressive activity of IL-10-secreting neutrophils differentiated with serum amyloid A. Nat Immunol 11(11):1039-46. [PubMed: 20890286]  [MGI Ref ID J:166540]

Del Cid N; Shen L; Belleisle J; Raghavan M. 2012. Assessment of roles for calreticulin in the cross-presentation of soluble and bead-associated antigens. PLoS One 7(7):e41727. [PubMed: 22848581]  [MGI Ref ID J:189698]

Ertelt JM; Buyukbasaran EZ; Jiang TT; Rowe JH; Xin L; Way SS. 2013. B7-1/B7-2 blockade overrides the activation of protective CD8 T cells stimulated in the absence of Foxp3+ regulatory T cells. J Leukoc Biol 94(2):367-76. [PubMed: 23744647]  [MGI Ref ID J:204423]

Ewald SJ; McKenzie IFC. 1979. Immunochemical characterization of the Ly-5 alloantigens on thymocytes. Immunogenetics 8:583-587.  [MGI Ref ID J:13367]

Felmy B; Songhet P; Slack EM; Muller AJ; Kremer M; Van Maele L; Cayet D; Heikenwalder M; Sirard JC; Hardt WD. 2013. NADPH oxidase deficient mice develop colitis and bacteremia upon infection with normally avirulent, TTSS-1- and TTSS-2-deficient Salmonella Typhimurium. PLoS One 8(10):e77204. [PubMed: 24143212]  [MGI Ref ID J:209119]

Florian MC; Nattamai KJ; Dorr K; Marka G; Uberle B; Vas V; Eckl C; Andra I; Schiemann M; Oostendorp RA; Scharffetter-Kochanek K; Kestler HA; Zheng Y; Geiger H. 2013. A canonical to non-canonical Wnt signalling switch in haematopoietic stem-cell ageing. Nature 503(7476):392-6. [PubMed: 24141946]  [MGI Ref ID J:206757]

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Glass AM; Wolf BJ; Schneider KM; Princiotta MF; Taffet SM. 2013. Connexin43 is dispensable for phagocytosis. J Immunol 190(9):4830-5. [PubMed: 23554311]  [MGI Ref ID J:195494]

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Hirahara K; Ghoreschi K; Yang XP; Takahashi H; Laurence A; Vahedi G; Sciume G; Hall AO; Dupont CD; Francisco LM; Chen Q; Tanaka M; Kanno Y; Sun HW; Sharpe AH; Hunter CA; O'Shea JJ. 2012. Interleukin-27 priming of T cells controls IL-17 production in trans via induction of the ligand PD-L1. Immunity 36(6):1017-30. [PubMed: 22726954]  [MGI Ref ID J:187415]

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Ng RL; Scott NM; Strickland DH; Gorman S; Grimbaldeston MA; Norval M; Waithman J; Hart PH. 2013. Altered immunity and dendritic cell activity in the periphery of mice after long-term engraftment with bone marrow from ultraviolet-irradiated mice. J Immunol 190(11):5471-84. [PubMed: 23636055]  [MGI Ref ID J:204767]

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Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryThe Mir223 locus is on the X chromosome. When maintaining a live colony, females homozygous for the Ptprca (CD45.1) allele and homozygous for the Mir223tm1Fcam allele may be bred with males homozygous for the Ptprca (CD45.1) allele and hemizygous for the Mir223tm1Fcam allele.
Mating SystemSee Colony Maintenance under the Health & Care tab         (Female x Male)   31-OCT-11

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $199.90MaleHomozygous for Ptprca, Hemizygous for Mir223tm1Fcam  
$199.90FemaleHomozygous for Ptprca, Homozygous for Mir223tm1Fcam  
Price per Pair (US dollars $)Pair Genotype
$399.80Homozygous for Ptprca, Homozygous for Mir223tm1Fcam x Homozygous for Ptprca, Hemizygous for Mir223tm1Fcam  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $259.90MaleHomozygous for Ptprca, Hemizygous for Mir223tm1Fcam  
$259.90FemaleHomozygous for Ptprca, Homozygous for Mir223tm1Fcam  
Price per Pair (US dollars $)Pair Genotype
$519.80Homozygous for Ptprca, Homozygous for Mir223tm1Fcam x Homozygous for Ptprca, Hemizygous for Mir223tm1Fcam  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   002014 B6.SJL-Ptprca Pepcb/BoyJ (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Tel: 1-800-422-6423 or 1-207-288-5845
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Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

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phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

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In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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