Strain Name:

C57BL/6N-H3f3btm1Psk/Mmjax

Availability:

Cryopreserved - Ready for recovery     Available at the JAX MMRRC

Please refer to the Mutant Mouse Regional Resource Center (MMRRC) for information about C57BL/6N-H3f3b/Mmjax MMRRC Stock Number 032055.
These H3f3bFL mutant mice possess loxP sites flanking exons 2-4 of the H3 histone, family 3b (H3f3b) gene. The basic nuclear protein, H3F3B, is one of the four core histones responsible for the nucleosome structure. This mutant mouse strain may be useful in studies of chromosomal structure and function.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Coisogenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating Investigator Paul Knoepfler,   UC Davis Genome Center

Description
These H3f3bFL mutant mice possess loxP sites flanking exons 2-4 of the H3 histone, family 3b (H3f3b) gene. The basic nuclear protein, H3F3B, is one of the four core histones responsible for the nucleosome structure. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. This mutant mouse strain may be useful in studies of chromosomal structure and function.

For example, when bred to mice carrying Tg(Zp3-cre)3Mrt, Cre recombinase expression in the oocyte results in reduced number of homozygotes, infertility, defective cell division and chromosome segregation.

Development
The targeting vector contains a FRT-flanked neomycin resistance cassette 3' of exon 1 followed by loxP sites flanking exons 2-4. The construct was electroporated into C57BL/6NTac-derived JM8.F6 embryonic stem (ES) cells. Correctly targeted ES cells were used to generate chimeric mice and the donating investigator reported that these mice were crossed to C57BL/6N mice (see SNP note below). Heterozygotes were intercrossed to generate a homozygous colony. Upon arrival, mice were bred to C57BL/6N for at least 1 generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, at least 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Control Information

  Control
   Wild-type from the colony (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of H3f3b
021789   129S-H3f3btm1.1Mnn/J
View Strains carrying other alleles of H3f3b     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

H3f3btm1Psk/H3f3btm1Psk

        involves: C57BL/6N
  • normal phenotype
  • no abnormal phenotype detected
    • mice exhibit normal viability, fecundity and fertility   (MGI Ref ID J:194593)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol H3f3btm1Psk
Allele Name targeted mutation 1, Paul Knoepfler
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) H3f3bFL;
Strain of OriginC57BL/6N
Gene Symbol and Name H3f3b, H3 histone, family 3B
Chromosome 11
Gene Common Name(s) 9430068D06Rik; H3.3A; H3.3B; H3F3; RIKEN cDNA 9430068D06 gene;
Molecular Note The targeting vector contains a FRT-flanked neomycin resistance cassette 3' of exon 1 followed by loxP sites flanking exons 2-4. [MGI Ref ID J:194593]
 
Gene Symbol and Name H3f3b, H3 histone, family 3B
Chromosome 11
Gene Common Name(s) 9430068D06Rik; H3.3A; H3.3B; H3F3; RIKEN cDNA 9430068D06 gene;

Genotyping

Genotyping Information

Genotyping Protocols

H3f3btm1Psk, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

H3f3btm1Psk related

Bush KM; Yuen BT; Barrilleaux BL; Riggs JW; O Geen H; Cotterman R; Knoepfler PS. 2013. Endogenous mammalian histone H3.3 exhibits chromatin-related functions during development. Epigenetics Chromatin 6(1):7. [PubMed: 23570311]  [MGI Ref ID J:194593]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhile maintaining a live colony, these mice are bred as homozygotes.

Pricing and Purchasing

Supply Notes


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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