Strain Name:

B6(Cg)-Abl1tm1Goff/J

Stock Number:

013225

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Availability:

Cryopreserved - Ready for recovery

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These Abl1m3 mutant mice possess a floxed neomycin resistance (neo) cassette, containing a stop codon, between exons 10 and 11 of the c-abl concogene 1, non-receptor tyrosine kinase gene, Abl1, resulting in low widespread detection of truncated protein. The allele also introduced five amino acid substitutions (Lysine to Glycine) in the first of three nuclear localization signals (NLS1) in exon 11. This strain may be useful for studying the growth, development, and physiology of cardiac tissue.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN8pN1
Generation Definitions
 
Donating Investigator Stephen P. Goff,   Columbia University

Description
These Abl1m3 mutant mice possess a floxed neomycin resistance (neo) cassette, containing a stop codon, between exons 10 and 11 of the c-abl concogene 1, non-receptor tyrosine kinase gene, Abl1, resulting in low widespread detection of truncated protein. The allele also introduced five amino acid substitutions (Lysine to Glycine) in the first of three nuclear localization signals (NLS1) in exon 11. Mice that are heterozygous for this allele are viable, fertile, and normal in size. Homozygotes exhibit perinatal death with enlarged hearts. When these Abl1m3 mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have the neo cassette deleted in the cre-expressing tissue, resulting in restoration of gene function while retaining the NLS1 mutations, and survival past birth. When bred to mice that express a cardiac-specific α-MHC-cre transgene (see Stock No. 011038), the mice exhibit decreased proliferation of cardiac tissue and die shortly after birth. This strain may be useful for studying the growth, development, and physiology of cardiac tissue.

Development
A targeting vector was designed to insert a loxP-flanked neomycin resistance (neo) cassette, containing a stop codon, between exons 10 and 11 of the c-abl concogene 1, non-receptor tyrosine kinase gene, Abl1. The construct also introduced five amino acid substitutions (Lysine to Glycine) in the first of three nuclear localization signals (NLS1) in exon 11. The construct was electroporated into albino B6(Cg)-Tyrc-2J/J-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric males were bred to B6(Cg)-Tyrc-2J/J females to establish a colony. These Abl1m3 mice were backcrossed to B6(Cg)-Tyrc-2J/J for at least eight generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony. The Jackson Laboratory bred black mice, and they may be segregating for the Tyrc-2J allele.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Abl1
009416   129S(B6)-Abl1tm1Mlg/J
013224   B6.129P2-Abl1tm2.1Goff/J
009417   B6.129S-Abl1tm1Mlg/J
024286   B6.129S4-Abl1tm1Ajk/J
009412   B6;129S-Abl1m1/J
View Strains carrying other alleles of Abl1     (5 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Abl1tm1Goff/Abl1tm1Goff

        involves: C57BL/6
  • mortality/aging
  • partial perinatal lethality
    • high levels of perinatal lethality   (MGI Ref ID J:156743)
    • mice are stated to be phenotypically indistinguishable from Abl1 null mice   (MGI Ref ID J:156743)
  • cardiovascular system phenotype
  • abnormal heart ventricle morphology
    • decrease in the size of the ventricular lumens   (MGI Ref ID J:156743)
    • thick ventricular wall   (MGI Ref ID J:156743)
  • enlarged heart   (MGI Ref ID J:156743)
    • heart hyperplasia   (MGI Ref ID J:156743)

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Abl1tm1Goff/Abl1tm1Goff Tg(Myh6-cre)2182Mds/0

        involves: C57BL/6   (conditional)
  • mortality/aging
  • complete neonatal lethality
    • most die shortly after birth   (MGI Ref ID J:156743)
  • cardiovascular system phenotype
  • *normal* cardiovascular system phenotype
    • compared to homozygous mutant mice not carrying the cre transgene, cardiomyocyte overproliferation and ventricular lumen size reduction are dramatically reduced   (MGI Ref ID J:156743)
    • thick ventricular wall
      • not as thick as in homozygous mutant mice not carrying the cre transgene but slightly thicker than in heterozygous controls   (MGI Ref ID J:156743)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cardiovascular Research
Heart Abnormalities

Internal/Organ Research
Heart Abnormalities

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Abl1tm1Goff
Allele Name targeted mutation 1, Stephen P Goff
Allele Type Targeted (Floxed/Frt)
Common Name(s) Abl1m3;
Mutation Made By Stephen Goff,   Columbia University
Strain of OriginC57BL/6
Gene Symbol and Name Abl1, c-abl oncogene 1, non-receptor tyrosine kinase
Chromosome 2
Gene Common Name(s) ABL; AI325092; E430008G22Rik; JTK7; RIKEN cDNA E430008G22 gene; bcr/abl; c-ABL; c-ABL1; expressed sequence AI325092; p150; v-abl;
Molecular Note A mutated NLS1with 5 amino acid substitutions (Lys to Gly) was introduced into exon 11 and a floxed neo cassette was introduced into intron 10 via homologous recombination. Western blot analysis revealed barely detectable levels of the full length mutant protein and low levels of a fragment consisting of the N terminal half. [MGI Ref ID J:156743]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Qiu Z; Cang Y; Goff SP. 2010. c-Abl tyrosine kinase regulates cardiac growth and development. Proc Natl Acad Sci U S A 107(3):1136-41. [PubMed: 20080568]  [MGI Ref ID J:156743]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, heterozygous mice may be bred to wildtype littermates or C57BL/6J inbred mice (Stock No. 000664).

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2085.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2710.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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