Strain Name:

B6;129S7-Dp(10Prmt2-Pdxk)2Yey/J

Stock Number:

013529

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This Dp(10)2Yey/+ mutant strain contains one copy of mouse Chromosome 10 with the targeted sequence duplicated between, and including, the protein arginine N-methyltransferase 2 (Prmt2) gene and the pyridoxal (pyridoxine, vitamin B6) kinase (Pdxk) gene. These mice may be useful for understanding developmental and cognitive disabilities associated with Down's Syndrome.

Description

Strain Information

Former Names B6;129S7-Dp(10Prmt2-Pdxk)1Yey/J    (Changed: 17-AUG-11 )
Type Duplication;
Additional information on Mice with Chromosomal Aberrations.
Type Congenic; Mutant Stock;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemNoncarrier x Hemizygote         (Female x Male)   20-SEP-12
Mating SystemHemizygote x Noncarrier         (Female x Male)   26-AUG-11
Specieslaboratory mouse
Generation?+N1F1 (15-MAY-13)
Generation Definitions
 
Donating Investigator Y. Eugene Yu,   Roswell Park Cancer Institute

Description
This Dp(10)2Yey/+ mutant strain contains one copy of mouse Chromosome 10 with the targeted sequence duplicated between, and including, the protein arginine N-methyltransferase 2 (Prmt2) gene and the pyridoxal (pyridoxine, vitamin B6) kinase (Pdxk) gene. Hemizygous mice are viable and fertile. This duplicated region on the mouse chromosome is one of three regions orthologous to an extra copy of human Chromosome 21 (Hsa21) which has been implicated in developmental cognitive disabilities like Down Syndrome (DS). Dp(10)1Yey/+ contains a duplication syntenic to the distal part of human 21q22.3, and carries 41 genes orthologous to genes on Hsa21. When mice carrying this duplication are bred to B6;129-Dp(16Lipi-Zfp295)1Yey/J mice (Stock No. 013530) and B6;129-Dp(17Abcg1-Rrp1b)3Yey/J mice (Stock No. 013531) to create a triple duplication model containing all three Hsa21 regions, the resulting mice exhibit DS related phenotypic defects. These defects include: reduction in body length and weight, hydrocephaly caused by aqueductal stenosis, impaired grip strength, impaired learning, and a deficit in long-term potentiation. 26% of triple duplication offspring die shortly after birth due to heart defects. These mice may be useful for understanding developmental and cognitive disabilities associated with DS.

Development
Chromosome-engineering cassettes were inserted into mouse Chromosome 10 of 129S7/SvEvBrd-Hprt1b-m2-derived AB2.2 embryonic stem (ES) cells, bracketing a span of approximately 2.3 Mb between, and including, the protein arginine N-methyltransferase 2 (Prmt2) gene and pyridoxal (pyridoxine, vitamin B6) kinase (Pdxk) loci. The cassette placed at the proximal locus was targeted upstream of Prmt2 and contained a neomycin resistance gene, a loxP site, a 5' portion of an HPRT minigene, and a tyrosinase minigene. The cassette placed at the distal locus was targeted downstream of the Pdxk gene and contained an agouti transgene, a 3' portion of an HPRT minigene, a loxP site and puromycin resistance gene. Double-targeted ES cells were transiently transfected with the cre recombinase expression vector pOG231. After subsequent selection of recombinants by using hypoxanthine aminopterin thymidine (HAT) media, correctly targeted ES cells, containing one copy of mouse Chromosome 10 with the targeted sequence duplicated (Dp), were injected into C57BL/6J-Tyrc-Brd blastocysts. The resulting chimeric mice were mated to C57BL/6J mice and were subsequently backcrossed to C57BL/6J for at least 3 generations to produce a colony of Dp(10)2Yey/+ mice. Upon arrival at The Jackson Laboratory these mice were bred to C57BL/6J mice (Stock No. 000664) for at least one generation to establish the colony.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Dp(10Prmt2-Pdxk)2Yey/0

        B6.129S7-Dp(10Prmt2-Pdxk)2Yey
  • mortality/aging
  • *normal* mortality/aging
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Craniofacial and Palate Defects
      hydrocephaly: severely abnormal skull
Skeletal Defects
      hydrocephaly

Neurobiology Research
Behavioral and Learning Defects
Down syndrome

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Dp(10Prmt2-Pdxk)2Yey
Allele Name duplication, Chr 10, Y Eugene Yu 2
Allele Type Targeted (knock-in)
Common Name(s) Dp(10)1Yey; Dp(10Prmt2-Pdxk)1Yey; Dp2Yey; Ts2Yey;
Strain of Origin129S7/SvEvBrd-Hprt
Expressed Gene Prmt2, protein arginine N-methyltransferase 2, mouse, laboratory
Expressed Gene Pdxk, pyridoxal (pyridoxine, vitamin B6) kinase, mouse, laboratory
Molecular Note Cre mediated chromosomal rearrangement between loxP sites in regions proximal to Prmt2 and distal to Pdxk results in duplication of the genes, including Prmt2 and Pdxk, in this approximately 2.3 Mb region that is syntenic with the distal part of human 21q22.3. [MGI Ref ID J:161325]
 
Gene Symbol and Name Dp(10Prmt2-Pdxk)2Yey, duplication, Chr 10, Y Eugene Yu 2
Chromosome 10
Gene Common Name(s) Dp(10)1Yey; Dp(10Prmt2-Pdxk)1Yey; Ts2Yey; duplication, Chr 10, Y Eugene Yu 1;
 

Genotyping

Genotyping Information

Genotyping Protocols

Dp(10Prmt2-Pdxk)2Yey,

Separated MCA


Dp(10Prmt2-Pdxk)2Yey, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Yu T; Li Z; Jia Z; Clapcote SJ; Liu C; Li S; Asrar S; Pao A; Chen R; Fan N; Carattini-Rivera S; Bechard AR; Spring S; Henkelman RM; Stoica G; Matsui S; Nowak NJ; Roder JC; Chen C; Bradley A; Yu YE. 2010. A mouse model of Down syndrome trisomic for all human chromosome 21 syntenic regions. Hum Mol Genet 19(14):2780-91. [PubMed: 20442137]  [MGI Ref ID J:161325]

Additional References

Dp(10Prmt2-Pdxk)2Yey related

Bhutta MF; Cheeseman MT; Herault Y; Yu YE; Brown SD. 2013. Surveying the Down syndrome mouse model resource identifies critical regions responsible for chronic otitis media. Mamm Genome :. [PubMed: 24068166]  [MGI Ref ID J:201811]

Yu T; Liu C; Belichenko P; Clapcote SJ; Li S; Pao A; Kleschevnikov A; Bechard AR; Asrar S; Chen R; Fan N; Zhou Z; Jia Z; Chen C; Roder JC; Liu B; Baldini A; Mobley WC; Yu YE. 2010. Effects of individual segmental trisomies of human chromosome 21 syntenic regions on hippocampal long-term potentiation and cognitive behaviors in mice. Brain Res 1366:162-71. [PubMed: 20932954]  [MGI Ref ID J:166931]

Zhang L; Fu D; Belichenko PV; Liu C; Kleschevnikov AM; Pao A; Liang P; Clapcote SJ; Mobley WC; Yu YE. 2012. Genetic analysis of Down syndrome facilitated by mouse chromosome engineering. Bioeng Bugs 3(1):8-12. [PubMed: 22126738]  [MGI Ref ID J:196862]

Zhang L; Meng K; Jiang X; Liu C; Pao A; Belichenko PV; Kleschevnikov AM; Josselyn S; Liang P; Ye P; Mobley WC; Yu YE. 2014. Human chromosome 21 orthologous region on mouse chromosome 17 is a major determinant of Down syndrome-related developmental cognitive deficits. Hum Mol Genet 23(3):578-89. [PubMed: 24041763]  [MGI Ref ID J:204610]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, hemizygous mice may be bred to non-carrier (wildtype) mice from the colony or to C57BL/6J inbred mice (Stock No. 000664).
Mating SystemNoncarrier x Hemizygote         (Female x Male)   20-SEP-12
Hemizygote x Noncarrier         (Female x Male)   26-AUG-11
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHemizygous for Dp(10Prmt2-Pdxk)2Yey  
Price per Pair (US dollars $)Pair Genotype
$302.00Hemizygous for Dp(10Prmt2-Pdxk)2Yey x Noncarrier  
$302.00Noncarrier x Hemizygous for Dp(10Prmt2-Pdxk)2Yey  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHemizygous for Dp(10Prmt2-Pdxk)2Yey  
Price per Pair (US dollars $)Pair Genotype
$392.60Hemizygous for Dp(10Prmt2-Pdxk)2Yey x Noncarrier  
$392.60Noncarrier x Hemizygous for Dp(10Prmt2-Pdxk)2Yey  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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