Strain Name:

B6.129P2-Hspa2tm1Dix/J

Stock Number:

013584

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
Male mice homozygous for this heat shock protein 2 knock out are infertile. This Hsp70-2 mutant strain may be useful in studies of infertility and meiosis.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.129-Hspa2tm1Dix/J    (Changed: 16-AUG-11 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationF1pN1
Generation Definitions
 
Donating Investigator Edward Mitch Eddy,   NIEHS-NIH

Description
Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous male mice are infertile, homozygous female mice are fertile. No gene product (mRNA) is detected by Northern blot analysis of testis tissue from homozygous male animals. Immunohistochemical examination of seminiferous tubules does not detect protein gene product. Homozygous males have testes that weigh significantly less than testes from controls. Spermatogenesis in homozygotes is arrested in prophase of meiosis I with a reduced number of postmeitotic spermatids in seminiferous tubules, many with abnormal morphology, and no spermatozoa in the epididymis. Apoptosis of germ cells (late pachytene spermatocytes) in the testes is greatly increased in mutants. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background.

Development
A targeting vector containing a PGK-Neo cassette was used to disrupt exon 2. The construct was electroporated into 129P2/OlaHsd derived E14TG2a/BK4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The donating investigator reports that these mice were backcrossed to C57BL/6 for more than 20 generations (see SNP note below). Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Hspa2
008870   C57BL/6-Tg(Hspa2-cre)1Eddy/J
View Strains carrying other alleles of Hspa2     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Hspa2tm1Dix/Hspa2tm1Dix

        either: 129S/SvEv or C57BL/6
  • endocrine/exocrine gland phenotype
  • decreased testis weight
    • testes of adult mutant males were significantly lower in weight as compared to heterozygous and wild-type controls   (MGI Ref ID J:35110)
    • epididymal and seminal vesicle weights were comparable to controls   (MGI Ref ID J:35110)
  • reproductive system phenotype
  • abnormal male meiosis
    • most seminiferous tubules contained pachytene spermatocytes with condensed nuclei, some of which appeared fragmented and apoptotic   (MGI Ref ID J:35110)
  • arrest of spermatogenesis
    • mice were deficient in postmeiotic spermatids and lacked spermatozoa in the epididymis   (MGI Ref ID J:35110)
  • decreased testis weight
    • testes of adult mutant males were significantly lower in weight as compared to heterozygous and wild-type controls   (MGI Ref ID J:35110)
    • epididymal and seminal vesicle weights were comparable to controls   (MGI Ref ID J:35110)
  • male infertility   (MGI Ref ID J:35110)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Reproductive Biology Research
Fertility Defects
      males only

Research Tools
Reproductive Biology Research
      male germ cells

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Hspa2tm1Dix
Allele Name targeted mutation 1, David J Dix
Allele Type Targeted (Null/Knockout)
Common Name(s) Hsp70-2-;
Mutation Made By Edward Mitch Eddy,   NIEHS-NIH
Strain of Origin129P2/OlaHsd
Gene Symbol and Name Hspa2, heat shock protein 2
Chromosome 12
Gene Common Name(s) 70kDa; HSP70-2; HSP70-3; Hsp70-2; Hspt70; Hst70; heat shock protein, 70 kDa 2;
Molecular Note The endogenous locus was disrupted by the insertion of a neomycin selection cassette inserted by homologous recombination. Transcript was undetected by Northern blot analysis of testicular tissue obtained from homozygous mutant mice. Immunostaining of testicular sections indicated an absence of normal protein in seminiferous tubules. [MGI Ref ID J:35110]

Genotyping

Genotyping Information

Genotyping Protocols

Hspa2tm1Dixalternate1,

SEPARATED MELT



Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Dix DJ; Allen JW; Collins BW; Mori C; Nakamura N; Poorman-Allen P; Goulding EH; Eddy EM. 1996. Targeted gene disruption of Hsp70-2 results in failed meiosis, germ cell apoptosis, and male infertility. Proc Natl Acad Sci U S A 93(8):3264-8. [PubMed: 8622925]  [MGI Ref ID J:35110]

Additional References

Hspa2tm1Dix related

Blackshear P; Mahler J; Bennett LM; McAllister KA; Forsythe D; Davis BJ. 1999. Extragonadal teratocarcinoma in chimeric mice. Vet Pathol 36(5):457-60. [PubMed: 10490215]  [MGI Ref ID J:59442]

Dix DJ; Allen JW; Collins BW; Poorman-Allen P; Mori C; Blizard DR; Brown PR; Goulding EH; Strong BD; Eddy EM. 1997. HSP70-2 is required for desynapsis of synaptonemal complexes during meiotic prophase in juvenile and adult mouse spermatocytes. Development 124(22):4595-603. [PubMed: 9409676]  [MGI Ref ID J:44767]

Eddy EM. 1998. HSP70-2 heat-shock protein of mouse spermatogenic cells. J Exp Zool 282(1-2):261-71. [PubMed: 9723183]  [MGI Ref ID J:49789]

Eddy EM. 1999. Role of heat shock protein HSP70-2 in spermatogenesis. Rev Reprod 4(1):23-30. [PubMed: 10051099]  [MGI Ref ID J:54304]

Mori C; Allen JW; Dix DJ; Nakamura N; Fujioka M; Toshimori K; Eddy EM. 1999. Completion of meiosis is not always required for acrosome formation in HSP70-2 null mice. Biol Reprod 61(3):813-22. [PubMed: 10456862]  [MGI Ref ID J:57058]

Mori C; Nakamura N; Dix DJ; Fujioka M; Nakagawa S; Shiota K ; Eddy EM. 1997. Morphological analysis of germ cell apoptosis during postnatal testis development in normal and Hsp 70-2 knockout mice. Dev Dyn 208(1):125-36. [PubMed: 8989527]  [MGI Ref ID J:37516]

Zhu D; Dix DJ; Eddy EM. 1997. HSP70-2 is required for CDC2 kinase activity in meiosis I of mouse spermatocytes. Development 124(15):3007-14. [PubMed: 9247342]  [MGI Ref ID J:77458]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as heterozygotes. Homozygous males are infertile.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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