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| These 3373 S-state Cre-sensitive (3373 3SCS) mice contain a construct designed to insert (from 5' to 3') a splice acceptor (SA) sequence, a loxP site, a puromycin selection cassette, an SV40 polyadenylation (polyA) cassette, another loxP site, tdTomato open reading frame (ORF), an internal ribosome entry site (IRES) fused to an enhanced green fluorescent protein (GFP) flanked by 3373 loxP sites, followed by a second polyA cassette. The loxP sites flanking the 5' polyA cassette are in opposite orientation to the loxP sites flanking the 3' IRES-GFP cassette, and all are modified allowing for only 10% of the recombination efficiency of native loxP sites. This vector was inserted into the Gt(ROSA)26Sor locus allowing for widespread expression. These 3373 3SCS mice are of general use for detecting cells that have expressed the Cre recombinase, for Cre recombinase-based lineage marking experiments, and in particular for applications in which it is useful to discriminate cells that differ in how much Cre recombinase they have
expressed.
Cre recombinase-dependent excision of IRES-GFP in the 3373 3SCS strain occurs at approximately one third the efficiency it does in the 5172 3SCS strain (Stock No. 013587) | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Gene Trap; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N5pN1
Generation DefinitionsDonating Investigator Nigel Killeen, Univ of California San Francisco (UCSF) Description
These 3373 3-state Cre-sensitive (3373 3SCS) mice contain a construct designed to insert (from 5' to 3') a floxed-STOP cassette, tdTomato open reading frame (ORF), and a floxed-internal ribosome entry site (IRES) fused to an enhanced green fluorescent protein (GFP) ORF. The IRES-eGFP element was flanked by 3373 mutant loxP sites (which recombine at 10% the efficiency of wildtype loxP sites). This vector was inserted into the Gt(ROSA)26Sor locus allowing for widespread expression. The floxed-STOP cassette causes termination of transcription and results in no expression of either fluorophore. When bred to mice that express Cre recombinase, offspring will have the floxed-STOP cassette deleted in the cre-expressing tissue(s). This deletion results in tdTomato and/or GFP expression depending on how much Cre-recombinase the cells express. When crossed with a strain expressing low amounts of Cre recombinase, partial recombination results in tdTomato+ GFP+ cells due to removal of the floxed-STOP. When crossed to strains with higher concentrations of cre, the 3373 mutant loxP sites flanking the IRES-GFP cassette will be recombined and will result in tdTomato+ cells with varying amounts of GFP expression due to removal of the IRES-GFP cassette. These 3373 3SCS mice are of general use for detecting cells that have expressed the Cre recombinase, for Cre recombinase-based lineage marking experiments, and in particular for applications in which it is useful to discriminate cells that differ in how much Cre recombinase they have expressed.Cre recombinase-dependent excision of IRES-GFP in the 3373 3SCS strain occurs at approximately one third the efficiency it does in the 5172 3SCS strain(Stock No. 013587)
Development
The 3373 reporter construct was designed to insert (from 5' to 3') a splice acceptor (SA) sequence, a loxP site, a puromycin selection cassette, an SV40 polyadenylation (polyA) cassette, another loxP site, tdTomato open reading frame (ORF), a loxP-flanked internal ribosome entry site (IRES) fused to an enhanced green fluorescent protein (GFP) ORF, followed by a second polyA cassette. The IRES-eGFP-polyA element was flanked by 3373 mutant loxP sites (which are recombined at 10% the efficiency of wildtype loxP sites). The loxP sites flanking the 5' polyA cassette are in opposite orientation to the loxP sites flanking the 3' IRES-GFP cassette. This vector is driven by the Gt(ROSA)26Sor promoter and was electroporated into 129P2/OlaHsd-derived E14Tg2A.4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6x(C57BL/6xDBA/2 F1) recipient blastocysts, and chimeric males were bred with C57BL/6 females. The donating investigator reports that these 3373 3-state Cre-sensitive (3373 3SCS) mice were backcrossed to C57BL/6J mice for at least 5 generations (see SNP note below). Upon arrival at The Jackson Laboratory, mutant mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Visit the Parkinson's Disease Resource site for helpful information on Parkinson's and research resources.
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Gt(ROSA)26Sortm1Nik/Gt(ROSA)26Sor+
involves: 129P2/OlaHsd * C57BL/6
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:159447)
This mouse can be used to support research in many areas including:
Neurobiology Research
Parkinson's Disease
cell markers
Research Tools
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gt(ROSA)26Sortm1Nik | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Nigel Killeen | ||
| Allele Type | Targeted (Reporter) | ||
| Strain of Origin | 129P2/OlaHsd | ||
| Site of Expression | Cre excision of the stop signal results in expression of tdTomato and/or GFP in cre-expressing tissues depending on how much Cre-recombinase the cells express. | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | The construct inserted into the locus contained (5' to 3') a splice acceptor, floxed stop cassette, tdTomato sequence, and an IRES-EGFP flanked by 3373 loxP sites. [MGI Ref ID J:159447] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1sor STD, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Klinger M; Chmura SA; Killeen N. 2010. Reporter Alleles that Inform on Differences in Cre Recombinase Expression. J Immunol :. [PubMed: 20427774] [MGI Ref ID J:159447]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
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Contact information
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| phone: | 207-288-6470 |
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JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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