Strain Name:

B6.129S-Atoh1tm6.1Hzo/J

Stock Number:

013595

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These Atoh1FLAG mice contain three c-terminal FLAG tags fused to the 3' end of the(Atoh1) gene.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Stock; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN8pN1
Generation Definitions
 
Donating Investigator Huda Zoghbi,   Baylor College of Medicine

Description
These Atoh1FLAG mice contain three c-terminal FLAG tags fused to the 3' end of the atonal homolog 1 (Atoh1) gene. Homozygous mice are viable and fertile. Atoh1FLAG expression is evident evident in all Atoh1 expressing cells including cerebellar granule neuron precursors (GNPs) where it binds the active transcriptional enhancer region of the GLI-Kruppel family member GLI2 (Gli2) gene. These mice may be useful for studying postnatal cerebellar development and regulation of Sonic Hedgehog-induced medulloblastoma.

Development
A targeting vector was designed to fuse three c-terminal FLAG tags to the 3' end of the atonal homolog 1 (Atoh1) gene, followed by a frt-flanked neomycin (neo) selection cassette in reverse orientation to the gene. This construct was electroporated into 129S7/SvEvBrd-Hprt1b-m2-derived AB2.2 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym mutant mice to remove the neo cassette. The resulting Atoh1FLAG mice were subsequently mated with inbred C57BL/6 mice for at least 8 generations. Upon arrival at The Jackson Laboratory, these mice were bred with C57BL/6J mice (Stock No. 000664) for at least one generation to establish the colony.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Atoh1
013593   B6.129S-Atoh1tm4.1Hzo/J
013594   B6.129S-Atoh1tm5.1(Cre/PGR)Hzo/J
005970   B6.129S7-Atoh1tm2Hzo/J
008681   B6.129S7-Atoh1tm3Hzo/J
011104   B6.Cg-Tg(Atoh1-cre)1Bfri/J
007684   STOCK Tg(Atoh1-cre/Esr1*)14Fsh/J
View Strains carrying other alleles of Atoh1     (6 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Atoh1tm6.1Hzo/Atoh1tm6.1Hzo

        involves: 129S7/SvEvBrd * C57BL/6 * SJL
  • normal phenotype
  • no abnormal phenotype detected
    • FLAG tagged protein functions as a wild-type version   (MGI Ref ID J:155047)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence

Neurobiology Research
Cerebellar Defects

Research Tools
Developmental Biology Research
Neurobiology Research
      cell marker

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Atoh1tm6.1Hzo
Allele Name targeted mutation 6.1, Huda Y Zoghbi
Allele Type Targeted
Common Name(s) Atoh1flag;
Mutation Made By Huda Zoghbi,   Baylor College of Medicine
Strain of Origin129S7/SvEvBrd-Hprt
Promoter Atoh1, atonal homolog 1 (Drosophila), mouse, laboratory
Molecular Note A cDNA encoding for a version of the gene with three C-terminal FLAG tags and an FRT-NEO-FRT selection cassette were inserted replacing the entire coding sequence. The selection cassette was then removed by flp mediated recombination. Expression of the tagged protein in the cerebellum but not the cortex was confirmed by chromatin immunoprecipitation analysis. [MGI Ref ID J:155047]

Genotyping

Genotyping Information

Genotyping Protocols

Atoh1tm6.1Hzo, QPCR
Atoh1tm6.1Hzoalternate1, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Flora A; Klisch TJ; Schuster G; Zoghbi HY. 2009. Deletion of Atoh1 disrupts Sonic Hedgehog signaling in the developing cerebellum and prevents medulloblastoma. Science 326(5958):1424-7. [PubMed: 19965762]  [MGI Ref ID J:155047]

Additional References

Atoh1tm6.1Hzo related

Kim TH; Li F; Ferreiro-Neira I; Ho LL; Luyten A; Nalapareddy K; Long H; Verzi M; Shivdasani RA. 2014. Broadly permissive intestinal chromatin underlies lateral inhibition and cell plasticity. Nature 506(7489):511-5. [PubMed: 24413398]  [MGI Ref ID J:207494]

Klisch TJ; Xi Y; Flora A; Wang L; Li W; Zoghbi HY. 2011. In vivo Atoh1 targetome reveals how a proneural transcription factor regulates cerebellar development. Proc Natl Acad Sci U S A 108(8):3288-93. [PubMed: 21300888]  [MGI Ref ID J:169298]

Lai HC; Klisch TJ; Roberts R; Zoghbi HY; Johnson JE. 2011. In vivo neuronal subtype-specific targets of atoh1 (math1) in dorsal spinal cord. J Neurosci 31(30):10859-71. [PubMed: 21795538]  [MGI Ref ID J:174506]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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