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| These Atoh1FLAG mice contain three c-terminal FLAG tags fused to the 3' end of the(Atoh1) gene. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Stock; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N8pN1
Generation DefinitionsDonating Investigator Huda Zoghbi, Baylor College of Medicine Description
These Atoh1FLAG mice contain three c-terminal FLAG tags fused to the 3' end of the atonal homolog 1 (Atoh1) gene. Homozygous mice are viable and fertile. Atoh1FLAG expression is evident evident in all Atoh1 expressing cells including cerebellar granule neuron precursors (GNPs) where it binds the active transcriptional enhancer region of the GLI-Kruppel family member GLI2 (Gli2) gene. These mice may be useful for studying postnatal cerebellar development and regulation of Sonic Hedgehog-induced medulloblastoma.Development
A targeting vector was designed to fuse three c-terminal FLAG tags to the 3' end of the atonal homolog 1 (Atoh1) gene, followed by a frt-flanked neomycin (neo) selection cassette in reverse orientation to the gene. This construct was electroporated into 129S7/SvEvBrd-Hprt1b-m2-derived AB2.2 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym mutant mice to remove the neo cassette. The resulting Atoh1FLAG mice were subsequently mated with inbred C57BL/6 mice for at least 8 generations. Upon arrival at The Jackson Laboratory, these mice were bred with C57BL/6J mice (Stock No. 000664) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Atoh1
013593 B6.129S-Atoh1tm4.1Hzo/J 013594 B6.129S-Atoh1tm5.1(Cre/PGR)Hzo/J 005970 B6.129S7-Atoh1tm2Hzo/J 008681 B6.129S7-Atoh1tm3Hzo/J 011104 B6.Cg-Tg(Atoh1-cre)1Bfri/J 007684 STOCK Tg(Atoh1-cre/Esr1*)14Fsh/J View Strains carrying other alleles of Atoh1 (6 strains)
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Atoh1tm6.1Hzo/Atoh1tm6.1Hzo
involves: 129S7/SvEvBrd * C57BL/6 * SJL
- normal phenotype
- no abnormal phenotype detected
- FLAG tagged protein functions as a wild-type version (MGI Ref ID J:155047)
This mouse can be used to support research in many areas including:
Cancer Research
Increased Tumor Incidence
Neurobiology Research
Cerebellar Defects
Research Tools
Developmental Biology Research
Neurobiology Research
cell marker
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Atoh1tm6.1Hzo | ||
|---|---|---|---|
| Allele Name | targeted mutation 6.1, Huda Y Zoghbi | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Atoh1flag; | ||
| Mutation Made By | Huda Zoghbi, Baylor College of Medicine | ||
| Strain of Origin | 129S7/SvEvBrd-Hprt | ||
| Promoter | Atoh1, atonal homolog 1 (Drosophila), mouse, laboratory | ||
| Molecular Note | A cDNA encoding for a version of the gene with three C-terminal FLAG tags and an FRT-NEO-FRT selection cassette were inserted replacing the entire coding sequence. The selection cassette was then removed by flp mediated recombination. Expression of the tagged protein in the cerebellum but not the cortex was confirmed by chromatin immunoprecipitation analysis. [MGI Ref ID J:155047] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Atoh1tm6.1Hzo, QPCR
Atoh1tm6.1Hzoalternate1, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Flora A; Klisch TJ; Schuster G; Zoghbi HY. 2009. Deletion of Atoh1 disrupts Sonic Hedgehog signaling in the developing cerebellum and prevents medulloblastoma. Science 326(5958):1424-7. [PubMed: 19965762] [MGI Ref ID J:155047]
Additional References
Atoh1tm6.1Hzo relatedKlisch TJ; Xi Y; Flora A; Wang L; Li W; Zoghbi HY. 2011. In vivo Atoh1 targetome reveals how a proneural transcription factor regulates cerebellar development. Proc Natl Acad Sci U S A 108(8):3288-93. [PubMed: 21300888] [MGI Ref ID J:169298]
Lai HC; Klisch TJ; Roberts R; Zoghbi HY; Johnson JE. 2011. In vivo neuronal subtype-specific targets of atoh1 (math1) in dorsal spinal cord. J Neurosci 31(30):10859-71. [PubMed: 21795538] [MGI Ref ID J:174506]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
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Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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