Strain Name:

B6;FVB-Tg(Myh6/NFAT-luc)1Jmol/J

Stock Number:

013781

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The NFAT-luc transgene has luciferase expression directed by a Myh6/NFAT hybrid promoter (composed of a minimal mouse alpha myosin heavy chain cardiac promoter [Myh6] with upstream copies of the nuclear factor of activated T cell binding site sequence [NFAT; from the mouse interleukin 4 (Il4) promoter]). In the hearts of adult NFAT-luc transgenic mice, NFAT-luc activity is upregulated during pathological cardiac hypertrophy induced by pressure-overload; more dramatically following myocardial infarction-induced heart failure. These NFAT-luc transgenic mice are a reporter of calcineurin-NFAT signaling, and may be useful in studying cardiac hypertrophy and calcineurin/NFAT coupling.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.FVB-Tg(Myh6/NFAT-luc)1Jmol/J    (Changed: 19-APR-12 )
Type Mutant Stock; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
GenerationN4pN1
Generation Definitions
 
Donating Investigator Jeffery D. Molkentin,   Cincinnati Children's Hospital

Description
These nuclear factor of activated T cell (NFAT)-luciferase transgenic mice display detectable luciferase activity in most tissues surveyed at 3 weeks of age, with highest expression occurring in brain, kidney, and heart - each of which are sites of considerable calcineurin (Ppp3ca, protein phosphatase 3, catalytic subunit, alpha isoform) protein expression. NFAT-luciferase activity peaks during developmental maturation of the heart, whereas the adult heart shows relatively lower activity. In the adult heart, NFAT-luciferase activity is upregulated in a delayed, but sustained manner throughout 8 weeks of pathological cardiac hypertrophy induced by pressure-overload, or more dramatically following myocardial infarction-induced heart failure. This strain may be useful in studies of cardiac hypertrophy and calcineurin/NFAT coupling.

Development
Nine copies of an NFAT binding site from the mouse Il4 (interleukin 4) promoter (5'-TGGAAAATT-3') were positioned 5' to a minimal Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter and inserted upstream of a luciferase reporter. The transgene was injected into FVB fertilized oocytes. This strain was backcrossed to C57BL/6 for four generations by the donating laboratory.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. 15 markers on 12 chromosomes were found to be segregating, suggesting an incomplete backcross.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Tg(Myh6/NFAT-luc)1Jmol allele
010588   FVB-Tg(Myh6/NFAT-luc)1Jmol/J
View Strains carrying   Tg(Myh6/NFAT-luc)1Jmol     (1 strain)

Strains carrying other alleles of Myh6
012389   B6.Cg-Tg(Myh6-Ppara)404-3Dpk/J
012382   B6.Cg-Tg(Myh6-Ppara)404-4Dpk/J
005657   B6.FVB(129)-Tg(Myh6-cre/Esr1*)1Jmk/J
011038   B6.FVB-Tg(Myh6-cre)2182Mds/J
002640   B6;SJL-Tg(Myh6-ADRBK1)27Wjk/J
012383   B6CBA-Tg(Myh6-Ppard)HEDpk/J
018972   B6N.FVB(B6)-Tg(Myh6-cre)2182Mds/J
002639   B6SJL-Tg(Myh6-ADRBK1)12Wjk/J
002638   B6SJL-Tg(WTbeta2)4Wjk/J
006768   D2.Cg-Tg(Myh6-Zfpm2)1Sho/EiJ
010587   FVB-Tg(Myh6-MEF2A)1Jmol/J
010581   FVB-Tg(Myh6-Map2k1*)1Jmol/J
010582   FVB-Tg(Myh6-Map2k3*)1Jmol/J
010584   FVB-Tg(Myh6-Map2k7)1Jmol/J
010585   FVB-Tg(Myh6-Mapk1)1Jmol/J
010583   FVB-Tg(Myh6-Mapk14*)1Jmol/J
010586   FVB-Tg(Myh6-Mef2c)2Jmol/J
009438   FVB-Tg(Myh6-SOD2,Tyr)3Pne/J
016570   FVB-Tg(Myh6-TRPC3*)6.6Jmol/J
017543   FVB-Tg(Myh6-TRPC6*)1Jmol/J
011037   FVB-Tg(Myh6-cre)2182Mds/J
017542   FVB-Tg(Myh6/tetO-ATP2B4)1Jmol/J
016571   FVB-Tg(Myh6/tetO-Gata6)2Jmol/J
014155   FVB-Tg(Myh6/tetO-Itpr1)22.3Jmol/J
014153   FVB-Tg(Myh6/tetO-Itpr2)3.11Jmol/J
012684   FVB-Tg(Myh6/tetO-POSTN)22.1Jmol/J
010580   FVB-Tg(Myh6/tetO-PRKCA*)1Jmk/J
022979   FVB-Tg(tetO-Thbs4)17.7Jmol/J
002535   FVB/N-Tg(ANX6)2Agh/J
012459   FVB/N-Tg(Myh6*/tetO-Capn1)L2Gwd/J
008716   FVB/N-Tg(Myh6-AIP/PLN*)46Jded/J
012461   FVB/N-Tg(Myh6-Cast)1Gwd/J
012460   FVB/N-Tg(Myh6-Gnaq)40Gwd/J
012477   STOCK Tg(Myh6*/tetO-GCaMP2)1Mik/J
009075   STOCK Tg(Myh6-Ppp3ca)37Eno/J
010579   STOCK Tg(Myh6-Prkca)1Jmk/J
009074   STOCK Tg(Myh6-cre)1Jmk/J
005650   STOCK Tg(Myh6-cre/Esr1*)1Jmk/J
016572   STOCK Tg(Myh6/tetO-Gata4)1Jmol/J
View Strains carrying other alleles of Myh6     (39 strains)

Strains carrying other alleles of luc
006102   B10.Cg-H2k Tg(Il2/NFAT-luc)83Rinc/J
006100   B10.Cg-H2k Tg(NFkB/Fos-luc)26Rinc/J
006852   B6.129S6-Per2tm1Jt/J
016187   B6.BTBR-Tg(Per1-luc,Per1)1Jt/J
003479   B6.C3-Tg(Fos-luc)1Rnd/J
006098   B6.Cg-Tg(Il2/NFAT-luc)83Rinc/J
006851   B6.Cg-Tg(Per1-luc)025Jt/J
016165   B6.Cg-Tg(Per1-luc)128Jt/J
016166   B6.Cg-Tg(Per1-luc)141Jt/J
005999   B6.Cg-Tg(SBE/TK-luc)7Twc/J
006101   B6.Cg-Tg(TRE/Prl-luc)31FlvRinc/J
025854   B6.FVB-Ptprca Tg(CAG-luc,-GFP)L2G85Chco Thy1a/J
011060   B6;129S4-Nanogtm1Jae/J
002709   B6;C3-Tg(TettTALuc)1Dgs/J
010545   C.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
012943   C57BL/6-Tg(Ins2-luc/EGFP/TK)300Kauf/J
010548   D1.FVB(Cg)-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
008450   FVB-Tg(CAG-luc,-GFP)L2G85Chco/J
009638   FVB-Tg(GFAP-luc,GAPDH-rluc)172.9Mes/J
018167   FVB-Tg(TERT-rluc)DJzhu/J
005125   FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J
006206   FVB.129S6-Gt(ROSA)26Sortm2(HIF1A/luc)Kael/J
002060   FVB/N-Tg(HIV-luc)326Morr/J
007800   FVB/N-Tg(Ins1-luc)VUPwrs/J
012370   FVB/NJ-Tg(Hspa1a-luc,-EGFP)2Chco/J
013116   NOD.B6-Tg(Ins2-luc/EGFP/TK)300Kauf/J
010542   NOD.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
017596   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Tg(SMN2)89Ahmb Smn1tm1Msd Tg(SMN2*delta7)4299Ahmb Tg(tetO-SMN2,-luc)#aAhmb/J
017597   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Tg(SMN2)89Ahmb Smn1tm1Msd Tg(SMN2*delta7)4299Ahmb Tg(tetO-SMN2,-luc)#bAhmb/J
003275   STOCK Tg(tetL)1Bjd/J
003274   STOCK Tg(tetNZL)2Bjd/J
017599   STOCK Tg(tetO-SMN2,-luc)#aAhmb/J
017600   STOCK Tg(tetO-SMN2,-luc)#bAhmb/J
View Strains carrying other alleles of luc     (33 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Tg(Myh6/NFAT-luc)1Jmol/0

        FVB-Tg(Myh6/NFAT-luc)1Jmol
  • normal phenotype
  • no abnormal phenotype detected
    • mice are viable and fertile   (MGI Ref ID J:83547)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cardiovascular Research
Fluorescent Proteins

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Myh6/NFAT-luc)1Jmol
Allele Name transgene insertion 1, Jeffery D Molkentin
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) NFAT-luc; Tg(NFAT luc reporter);
Strain of OriginFVB
Site of ExpressionLuciferase expression is seen in the heart.
Expressed Gene luc, luciferase, firefly
Promoter Myh6/NFAT, Myh6/NFAT hybrid promoter (myosin, heavy polypeptide 6, cardiac muscle, alpha minimal promoter with nine upstream copies of the nuclear factor of activated T cell binding site sequence [NFAT; from the mouse interleukin 4 promoter]).,
Promoter Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, murine, murine
Molecular Note Nine copies of a nuclear factor of activated T cell (NFAT) binding site (5'-TGGAAAATT-3') from the interleukin 4 (Il4) promoter were placed 5' to a mouse alpha myosin heavy chain (Myh6) and upstream of a luciferase promoter. [MGI Ref ID J:83547]
 
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Myh6/NFAT-luc), Melt Curve Analysis
Tg(Myh6/NFAT-luc), Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Wilkins BJ; Dai YS; Bueno OF; Parsons SA; Xu J; Plank DM; Jones F; Kimball TR; Molkentin JD. 2004. Calcineurin/NFAT coupling participates in pathological, but not physiological, cardiac hypertrophy. Circ Res 94(1):110-8. [PubMed: 14656927]  [MGI Ref ID J:149731]

Additional References

Tg(Myh6/NFAT-luc)1Jmol related

Bierer R; Nitta CH; Friedman J; Codianni S; de Frutos S; Dominguez-Bautista JA; Howard TA; Resta TC; Bosc LV. 2011. NFATc3 is required for chronic hypoxia-induced pulmonary hypertension in adult and neonatal mice. Am J Physiol Lung Cell Mol Physiol 301(6):L872-80. [PubMed: 21908592]  [MGI Ref ID J:180028]

Braz JC; Bueno OF; Liang Q; Wilkins BJ; Dai YS; Parsons S; Braunwart J; Glascock BJ; Klevitsky R; Kimball TF; Hewett TE; Molkentin JD. 2003. Targeted inhibition of p38 MAPK promotes hypertrophic cardiomyopathy through upregulation of calcineurin-NFAT signaling. J Clin Invest 111(10):1475-86. [PubMed: 12750397]  [MGI Ref ID J:83547]

Gelpi RJ; Gao S; Zhai P; Yan L; Hong C; Danridge LM; Ge H; Maejima Y; Donato M; Yokota M; Molkentin JD; Vatner DE; Vatner SF; Sadoshima J. 2009. Genetic inhibition of calcineurin induces diastolic dysfunction in mice with chronic pressure overload. Am J Physiol Heart Circ Physiol 297(5):H1814-9. [PubMed: 19717730]  [MGI Ref ID J:154325]

Jiang DS; Wei X; Zhang XF; Liu Y; Zhang Y; Chen K; Gao L; Zhou H; Zhu XH; Liu PP; Bond Lau W; Ma X; Zou Y; Zhang XD; Fan GC; Li H. 2014. IRF8 suppresses pathological cardiac remodelling by inhibiting calcineurin signalling. Nat Commun 5:3303. [PubMed: 24526256]  [MGI Ref ID J:206811]

Kritzer MD; Li J; Passariello CL; Gayanilo M; Thakur H; Dayan J; Dodge-Kafka K; Kapiloff MS. 2014. The scaffold protein muscle A-kinase anchoring protein beta orchestrates cardiac myocyte hypertrophic signaling required for the development of heart failure. Circ Heart Fail 7(4):663-72. [PubMed: 24812305]  [MGI Ref ID J:214288]

Ramiro-Diaz JM; Nitta CH; Maston LD; Codianni S; Giermakowska W; Resta TC; Bosc LV. 2013. NFAT is required for spontaneous pulmonary hypertension in superoxide dismutase 1 knockout mice. Am J Physiol Lung Cell Mol Physiol 304(9):L613-25. [PubMed: 23475768]  [MGI Ref ID J:196362]

de Frutos S; Caldwell E; Nitta CH; Kanagy NL; Wang J; Wang W; Walker MK; Gonzalez Bosc LV. 2010. NFATc3 contributes to intermittent hypoxia-induced arterial remodeling in mice. Am J Physiol Heart Circ Physiol 299(2):H356-63. [PubMed: 20495147]  [MGI Ref ID J:163869]

de Frutos S; Diaz JM; Nitta CH; Sherpa ML; Bosc LV. 2011. Endothelin-1 contributes to increased NFATc3 activation by chronic hypoxia in pulmonary arteries. Am J Physiol Cell Physiol 301(2):C441-50. [PubMed: 21525433]  [MGI Ref ID J:175668]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, hemizygotes may be bred with wildtype mice.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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