Strain Name:

B6.129S2-Rbfox1tm1.1Dblk/J

Stock Number:

014089

Order this mouse

Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These mutant mice possess loxP sites flanking exons 11-12 of the Rbfox1 gene. This strain may be useful in generating conditional mutations for studying the role of Fox-1 in development and physiology of the brain, heart, and muscle tissues.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN6pN1
Generation Definitions
 
Donating Investigator Douglas Black,   UCLA

Description
These Fox1flox mutant mice possess loxP sites flanking exons 11-12 of the RNA binding protein, fox-1 homolog 1 (Rbfox1) gene. Mice that are homozygous for this allele are viable, fertile, and normal in size. Fox-1 is expressed in brain, heart, and skeletal muscle and regulates alternative splicing in vertebrates by binding specifically to (U)GCAUG sequences. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exons 11-12 deleted in the Cre-expressing tissue, resulting in inactivation of Fox-1 gene function.

For example, when crossed to a strain expressing Cre recombinase in the central and peripheral nervous system (see Stock No. 003771), this mutant mouse strain may be useful in studies of neuronal excitation and seizures.

When bred to mice carrying Tg(Pcp2-cre)2Mpin (Stock No. 004146) and Rbfox2tm1.1Dblk (Stock No. 014090) , Cre recombinase expression in the Purkinje cells may be useful in studies of Purkinje cell pacemaking.

Development
A targeting vector was designed to insert loxP sites flanking exons 11-12 of the RNA binding protein, fox-1 homolog 1 (Rbfox1) gene, followed by a frt-flanked neomycin resistance (neo) cassette. The construct was electroporated into 129S2/Sv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and resulting chimeric males were bred to C57BL/6J females. Offspring were bred with 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J transgenic mice (Stock No. 003946) to delete the neo cassette, resulting in a colony of Fox-1flox mice. The donating investigator reported that the resulting offspring were then backcrossed to C57BL/6J mice for at least 6 generations (see SNP note below). Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Rbfox1tm1.1Dblk/Rbfox1+ Rbfox2tm1.1Dblk/Rbfox2tm1.1Dblk Tg(Nes-cre)1Kln/0

        involves: 129S2/SvPas * C57BL/6 * C57BL/6J * SJL   (conditional)
  • mortality/aging
  • complete postnatal lethality
    • euthanasia due to immobility by 3?4 weeks of age   (MGI Ref ID J:181631)
  • nervous system phenotype
  • abnormal neuron physiology
    • Purkinje cells show a marked decrease in firing frequency   (MGI Ref ID J:181631)
  • ectopic Purkinje cell   (MGI Ref ID J:181631)
  • small cerebellum   (MGI Ref ID J:181631)
  • behavior/neurological phenotype
  • ataxia
    • develop severe ataxia by 2 weeks of age becoming immobile by 3-4 weeks of age   (MGI Ref ID J:181631)
  • growth/size/body phenotype
  • decreased body size

Rbfox1tm1.1Dblk/Rbfox1+ Tg(Nes-cre)1Kln/0

        involves: 129S2/SvPas * 129S4/SvJaeSor * C57BL/6 * C57BL/6J * SJL   (conditional)
  • mortality/aging
  • increased sensitivity to xenobiotic induced morbidity/mortality
    • in mice treated with kainic acid   (MGI Ref ID J:176189)
  • nervous system phenotype
  • increased susceptibility to pharmacologically induced seizures
    • increased incidence resulting in death in response to kainic acid treatment   (MGI Ref ID J:176189)
  • behavior/neurological phenotype
  • increased susceptibility to pharmacologically induced seizures
    • increased incidence resulting in death in response to kainic acid treatment   (MGI Ref ID J:176189)
  • homeostasis/metabolism phenotype
  • increased sensitivity to xenobiotic induced morbidity/mortality
    • in mice treated with kainic acid   (MGI Ref ID J:176189)

Rbfox1tm1.1Dblk/Rbfox1tm1.1Dblk Rbfox2tm1.1Dblk/Rbfox2tm1.1Dblk Tg(Nes-cre)1Kln/0

        involves: 129S2/SvPas * C57BL/6 * C57BL/6J * SJL   (conditional)
  • mortality/aging
  • complete perinatal lethality   (MGI Ref ID J:181631)

Rbfox1tm1.1Dblk/Rbfox1tm1.1Dblk Rbfox2tm1.1Dblk/Rbfox2tm1.1Dblk Tg(Pcp2-cre)2Mpin/0

        involves: 129 * C57BL/6J   (conditional)
  • behavior/neurological phenotype
  • *normal* behavior/neurological phenotype
    • do not display ataxia   (MGI Ref ID J:181631)
    • impaired coordination
      • shorter mean latency to fall in the first trial and performance improves only slightly over subsequent trials   (MGI Ref ID J:181631)
  • nervous system phenotype
  • *normal* nervous system phenotype
    • do not display defects in cerebellar development   (MGI Ref ID J:181631)
    • abnormal neuron physiology
      • at P70, but not at P20, Purkinje cells show a decrease in firing frequency   (MGI Ref ID J:181631)
      • at P70 Purkinje cells show an increase in variability of firing   (MGI Ref ID J:181631)

Rbfox1tm1.1Dblk/Rbfox1tm1.1Dblk Tg(Nes-cre)1Kln/0

        involves: 129S2/SvPas * 129S4/SvJaeSor * C57BL/6 * C57BL/6J * SJL   (conditional)
  • mortality/aging
  • increased sensitivity to xenobiotic induced morbidity/mortality
    • in mice treated with kainic acid   (MGI Ref ID J:176189)
  • reproductive system phenotype
  • reduced fertility   (MGI Ref ID J:176189)
  • nervous system phenotype
  • *normal* nervous system phenotype
    • mice exhibit normal gross brain morphology   (MGI Ref ID J:176189)
    • abnormal dendritic spine morphology
      • very modest decrease in spine density in the dentate gyrus   (MGI Ref ID J:176189)
    • abnormal excitatory postsynaptic potential
      • mice require lower stimulus intensities to evoke field excitatory postsynaptic potentials compared with control mice   (MGI Ref ID J:176189)
    • seizures
      • infrequent spontaneous seizures   (MGI Ref ID J:176189)
      • increased susceptibility to pharmacologically induced seizures
        • increased incidence and duration of tonic-clonic seizures resulting in death in response to kainic acid treatment   (MGI Ref ID J:176189)
      • tonic-clonic seizures
        • in mice treated with kainic acid   (MGI Ref ID J:176189)
  • growth/size/body phenotype
  • decreased body size
  • behavior/neurological phenotype
  • seizures
    • infrequent spontaneous seizures   (MGI Ref ID J:176189)
    • increased susceptibility to pharmacologically induced seizures
      • increased incidence and duration of tonic-clonic seizures resulting in death in response to kainic acid treatment   (MGI Ref ID J:176189)
    • tonic-clonic seizures
      • in mice treated with kainic acid   (MGI Ref ID J:176189)
  • homeostasis/metabolism phenotype
  • increased sensitivity to xenobiotic induced morbidity/mortality
    • in mice treated with kainic acid   (MGI Ref ID J:176189)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Internal/Organ Defects
      brain
      heart

Research Tools
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Rbfox1tm1.1Dblk
Allele Name targeted mutation 1.1, Douglas Black
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Fox-1flox; Rbfox1loxP;
Mutation Made By Douglas Black,   UCLA
Strain of Origin129S2/SvPas
Gene Symbol and Name Rbfox1, RNA binding protein, fox-1 homolog (C. elegans) 1
Chromosome 16
Gene Common Name(s) 2BP1; A2BP1; A2bp; A2bp1; FOX-1; FOX1; HRNBP1; ataxin 2 binding protein 1;
Molecular Note A targeting vector was designed to insert loxP sites flanking exons 11-12 of the RNA binding protein, fox-1 homolog 1 (Rbfox1) gene, followed by a frt-flanked neomycin resistance (neo) cassette. Flp-mediated recombination removed the neo cassette leavingexons 11 and 12 floxed. [MGI Ref ID J:165698]

Genotyping

Genotyping Information

Genotyping Protocols

Rbfox1tm1.1Dblk, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Black D. 2010. Direct Data Submission 2010/11/09 MGI Direct Data Submission :.  [MGI Ref ID J:165698]

Additional References

Rbfox1tm1.1Dblk related

Gehman LT; Meera P; Stoilov P; Shiue L; O'Brien JE; Meisler MH; Ares M Jr; Otis TS; Black DL. 2012. The splicing regulator Rbfox2 is required for both cerebellar development and mature motor function. Genes Dev 26(5):445-60. [PubMed: 22357600]  [MGI Ref ID J:181631]

Gehman LT; Stoilov P; Maguire J; Damianov A; Lin CH; Shiue L; Ares M Jr; Mody I; Black DL. 2011. The splicing regulator Rbfox1 (A2BP1) controls neuronal excitation in the mammalian brain. Nat Genet 43(7):706-11. [PubMed: 21623373]  [MGI Ref ID J:176189]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.6)