Strain Name:

STOCK Mkxtm1.2Jian/J

Stock Number:

014120

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Availability:

Cryopreserved - Ready for recovery

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This Mohawk homeobox targeted mutation mouse strain exhibits defects in tendon development and tendon collagen fibrillogenesis, and may be useful in studies of connective tissue and musculo-skeletal development.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating Investigator Rulang Jiang,   University of Rochester

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross behavioral abnormalities. By 2 weeks of age, homozygotes have wavy tails that are obvious when the mice are running. In the tails of homozygotes, tendon morphology is abnormal (crimp patterning), smaller in size than wildtype controls and tendon insertions on vertebrae are not easily seen. Limb tendons are also smaller with abnormal morphology. Tendon fibrils from mutants exhibit smaller diameter when compared to wildtype controls as early as postnatal day 5. Tendon sheaths from homozygotes are thicker and have more cell layers than wildtype controls.

Development
A targeting vector containing a FRT-site flanked PGK-Neo cassette was inserted downstream of exon 3 and loxP sites were inserted to flank exon 3. The construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+ derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting male chimeric animals were crossed to 129/SvJ female mice. The mice were then crossed to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J (STOCK no. 3946) mice to remove the FRT-flanked Neo cassette, and to B6.FVB-Tg(EIIa-cre)C5379Lmgd/J (STOCK no. 3724) to remove exon 3. Mice that no longer carried the PGK-Neo cassette or exon 3 were intercrossed to generate homozygotes. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Mkxtm1.2Jian/Mkxtm1.2Jian

        involves: 129S1/Sv * 129S4/SvJae * C57BL/6 * FVB/N
  • limbs/digits/tail phenotype
  • abnormal tail morphology
    • mice exhibit a wavy tail by 2 weeks of age; phenotype is more noticeable during movement   (MGI Ref ID J:175747)
  • muscle phenotype
  • abnormal tendon morphology
    • tail tendons of 2 week old mice appear smaller and less vibrant than wild-type   (MGI Ref ID J:175747)
    • tendon crimp (irregular undulations of collagen fibers that result in a banding pattern) is missing in tail tendons   (MGI Ref ID J:175747)
    • tail tendon insertions are fainter and less conspicuous than wild-type   (MGI Ref ID J:175747)
    • first observed at P9, the diameters of collagen fibrils in tail tendons are smaller and more uniform than wild-type   (MGI Ref ID J:175747)
    • tendon cell numbers are reduced in some (flexor digitorium profundus and digitorium communis and ), but not all of tendons   (MGI Ref ID J:175747)
    • limb tendons also are smaller and less vibrant, but in contrast to tail tendons exhibit tendon crimp, however, banding is more condensed than in wild-type   (MGI Ref ID J:175747)
    • tendon sheaths are thicker and contain more cell layers by 2 weeks of age as compared to wild-type   (MGI Ref ID J:175747)
  • skeleton phenotype
  • abnormal tendon morphology
    • tail tendons of 2 week old mice appear smaller and less vibrant than wild-type   (MGI Ref ID J:175747)
    • tendon crimp (irregular undulations of collagen fibers that result in a banding pattern) is missing in tail tendons   (MGI Ref ID J:175747)
    • tail tendon insertions are fainter and less conspicuous than wild-type   (MGI Ref ID J:175747)
    • first observed at P9, the diameters of collagen fibrils in tail tendons are smaller and more uniform than wild-type   (MGI Ref ID J:175747)
    • tendon cell numbers are reduced in some (flexor digitorium profundus and digitorium communis and ), but not all of tendons   (MGI Ref ID J:175747)
    • limb tendons also are smaller and less vibrant, but in contrast to tail tendons exhibit tendon crimp, however, banding is more condensed than in wild-type   (MGI Ref ID J:175747)
    • tendon sheaths are thicker and contain more cell layers by 2 weeks of age as compared to wild-type   (MGI Ref ID J:175747)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Skeletal Defects

Internal/Organ Research
Skeleton

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Mkxtm1.2Jian
Allele Name targeted mutation 1.2, Rulang Jiang
Allele Type Targeted (knock-out)
Common Name(s) Mkx-;
Mutation Made By Rulang Jiang,   University of Rochester
Strain of Origin129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name Mkx, mohawk homeobox
Chromosome 18
Gene Common Name(s) 9430023B20Rik; C10orf48; IFRX; IRXL1; Irxl1; RGD1305652; RIKEN cDNA 9430023B20 gene; iroquois homeobox protein-like 1;
Molecular Note Cre-mediated recombination removed exon 3 from Mkxtm1.1Jian. [MGI Ref ID J:175747]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Liu W; Watson SS; Lan Y; Keene DR; Ovitt CE; Liu H; Schweitzer R; Jiang R. 2010. The atypical homeodomain transcription factor Mohawk controls tendon morphogenesis. Mol Cell Biol 30(20):4797-807. [PubMed: 20696843]  [MGI Ref ID J:175747]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2085.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2710.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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