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| These Prkaa2 mutant mice possess loxP sites flanking exon 2 of the protein kinase, AMP-activated, alpha 2 catalytic subunit (Prkaa2) gene. This strain may be useful for studying cell growth and energy expenditure, as well as bladder cancer. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names B6.Cg-Prkaa2tm1.1Sjm/J (Changed: 28-DEC-10 ) Type Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 20-OCT-11 Species laboratory mouse Generation N5+
Generation DefinitionsDonating Investigator Sean J Morrison, UT Southwestern Description
These Prkaa2 mutant mice possess loxP sites flanking exon 2 of the protein kinase, AMP-activated, alpha 2 catalytic subunit (Prkaa2) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Prkaa2 belongs to the serine/threonine protein kinase family and is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a sensor of the energy status of cells and ensures survival during stress. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues. When bred to transgenic mice expressing Cre recombinase driven by myxovirus (influenza virus) resistance 1 (Mx1) promoter/enhanced elements, PRKAA2 expression is abolished in hematopoietic cells. This strain may be useful for studying cell growth and energy expenditure, as well as bladder cancer.Development
A targeting vector was designed to insert a loxP site upstream of exon 2, and a Frt-flanked neomycin resistance cassette (neo) followed by a second loxP site downstream of exon 2 of the protein kinase, AMP-activated, alpha 2 catalytic subunit (Prkaa2) gene. The construct was electroporated into Bruce4-derived C57BL/6 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts from B6(Cg)-Tyrc-2J/J mice (Stock No. 000058) and the resulting chimeric males were bred to female B6(Cg)-Tyrc-2J/J mice to establish a colony. These mice were then bred to mice carrying Tg(ACTFLPe)9205Dym to delete the neo cassette. Offspring were then backcrossed to C57BL/6 for at least 4 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:
Cancer Research
Cre-lox System
loxP-flanked Sequences
Cell Biology Research
Genes Regulating Growth and Proliferation
Developmental Biology Research
Growth Defects
Research Tools
Cancer Research
Cre-lox System
Cre-lox System
loxP-flanked Sequences
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Prkaa2tm1.1Sjm | ||
|---|---|---|---|
| Allele Name | targeted mutation 1.1, Sean Morrison | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | Ampka2fl; Prkaa2fl; | ||
| Mutation Made By | Sean Morrison, UT Southwestern | ||
| Strain of Origin | B6.Cg-Thy1 | ||
| Gene Symbol and Name | Prkaa2, protein kinase, AMP-activated, alpha 2 catalytic subunit | ||
| Chromosome | 4 | ||
| Gene Common Name(s) | 2310008I11Rik; AMPK; AMPK2; AMPKa2; AMPKalpha2; PRKAA; RIKEN cDNA 2310008I11 gene; | ||
| Molecular Note | A loxP site was inserted upstream of exon 2 and an FRT-flanked neo cassette with a 3' loxP site was inserted downstream of exon 2. Flp-mediated recombination removed the neo selection cassette. [MGI Ref ID J:165090] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Prkaa2tm1Sjm, Melt Curve Analysis
Prkaa2tm1Sjm, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Nakada D, Saunders TL, Morrison SJ. 2010. Lkb1 regulates cell cycle and energy metabolism in hematopoietic stem cells Nature 468(7324):653-658. [PubMed: 21124450] [MGI Ref ID J:165090]
Additional References
Prkaa2tm1.1Sjm relatedMeares GP; Qin H; Liu Y; Holdbrooks AT; Benveniste EN. 2013. AMP-Activated Protein Kinase Restricts IFN-gamma Signaling. J Immunol 190(1):372-80. [PubMed: 23180823] [MGI Ref ID J:190838]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together. Mating System Homozygote x Homozygote (Female x Male) 20-OCT-11 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Homozygous for Prkaa2tm1.1Sjm
Price per Pair (US dollars $) Pair Genotype $464.00 Homozygous for Prkaa2tm1.1Sjm x Homozygous for Prkaa2tm1.1Sjm Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Homozygous for Prkaa2tm1.1Sjm
Price per Pair (US dollars $) Pair Genotype $603.20 Homozygous for Prkaa2tm1.1Sjm x Homozygous for Prkaa2tm1.1Sjm Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
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Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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