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| This mutant strain lacks Eif2c2 catalytic activity and may be useful in studies of microRNA biogenesis and erythropoiesis. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6;129S6-Eif2c2tm3Ghan/J (Changed: 18-FEB-13 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N5pN1
Generation DefinitionsDonating Investigator Gregory Hannon, Cold Spring Harbor Laboratory Description
A point mutation in the Eif2c2 (eukaryotic translation initiation factor 2C, 2; also called Ago2) gene inactivates catalytic activity in this strain but does not disrupt gene expression. Homozygous neonates are anemic and die shortly after birth. Mice show a loss of miR-451, a small RNA important for erythropoiesis. Heterozygous targeted mutant mice are viable and fertile. There are no known phenotypic differences between this mouse carrying the floxed neo cassette and the strain from which the cassette has been deleted (see Stock No. 14151). This strain may be useful in studies of microRNA biogenesis and erythropoiesis.Development
An A to C mutation (D597A) was introduced to exon 14 and a loxP-flanked PGK-gb2-neo cassette (encoding neomycin/kanamycin resistance) was placed in intron 2. The point mutation creates an ADH rather than a DDH motif that disrupts catalytic activity, but not gene expression. The mutation was created in G4 (129S6/SvEvTac x C57BL/6Ncr)F1-derived embryonic stem cells. The strain was backcrossed to C57BL/6J for 3-4 generations by the donating laboratory.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Ago2
016520 B6.129P2(129S4)-Ago2tm1.1Tara/J 014151 STOCK Ago2tm3.1Ghan/J View Strains carrying other alleles of Ago2 (2 strains)
Phenotype
Phenotype Information
assigned by genotype
Ago2tm3Ghan/Ago2+
involves: 129S6/SvEvTac * C57BL/6NCr
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:161968)
This mouse can be used to support research in many areas including:
Developmental Biology Research
Postnatal Lethality
Homozygous
Hematological Research
Hematopoietic Defects
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Ago2tm3Ghan | ||
|---|---|---|---|
| Allele Name | targeted mutation 3, Greggory J Hannon | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Ago2ADH-NEO; Ago2D597A-NEO; | ||
| Mutation Made By | Gregory Hannon, Cold Spring Harbor Laboratory | ||
| Strain of Origin | (129S6/SvEvTac x C57BL/6NCr)F1 | ||
| Promoter | Ago2, argonaute RISC catalytic subunit 2, mouse, laboratory | ||
| Molecular Note | Mice exhibit an A to C mutation that results in the amino acid substitution of alanine for aspartic acid at position 598 (D598A) within the catalytic DDH motif. [MGI Ref ID J:161968] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Eif2c2tm3Ghan, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Cheloufi S; Dos Santos CO; Chong MM; Hannon GJ. 2010. A dicer-independent miRNA biogenesis pathway that requires Ago catalysis. Nature 465(7298):584-9. [PubMed: 20424607] [MGI Ref ID J:161968]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintained as a live colony, heterozygotes may be bred. Homozygotes die as neonates.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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