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| Transgenic UGT1A1*28 mice carry the entire human uridine diphosphate (UDP) glucuronosyltransferase 1 (UGT1) locus, and includes a mutant form of the human UGT1 polypeptide A1 (UGT1A1) promoter. These mice may be useful for studying the regulatory and functional properties of glucuronidation, and may also serve as a model for pharmacological studies associated with Gilbert's syndrome. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N5pN1
Generation DefinitionsDonating Investigator Robert Tukey, University of California San Diego Description
Transgenic UGT1A1*28 mice carry the entire human uridine diphosphate (UDP) glucuronosyltransferase 1 (UGT1) locus, and includes a mutant form of the human UGT1 polypeptide A1 (UGT1A1) promoter. Hemizygous mice are viable, fertile, and normal in size. The UGT1 locus encodes a family of genes, including UGT1A1-UGT1A10. UGT1 contains a series of divergent exon 1 sequences, each encoding the substrate binding site of a different UGT1A protein, and exons 2-5 which encode the highly conserved carboxyl terminal. Each exon 1 is regulated by its own promoter/enhancer sequences. The UGT1A1*28 mutation is associated with hepatic dysfunction and increased bilirubin found in Gilbert's syndrome. UGTs detoxify small lipophilic molecules and transform them into hydrophilic glucuronides, facilitating excretion. UGT1A1*28 transgenic mice express human UGT1A genes in patterns similar to the human tissues, mainly in tissues such as liver, kidney, and gastrointestinal tract. These mice may be useful for studying the regulatory and functional properties of glucuronidation, and may also serve as a model for pharmacological studies associated with Gilbert's syndrome.Development
A transgene derived from a human bacterial artificial chromosome (BAC) library, containing the entire human UGT1 (UDP glucuronosyltransferase 1) complex, was modified by targeted mutation of the UGT1 polypeptide A1, UGT1A1, promoter to harbor the UGT1A1*28 mutation associated with hepatic dysfunction found in Gilbert's syndrome. Specifically, this mutation inserts an extra TA in the TATA box sequence of the UGTA1A promoter. This transgene was microinjected into fertilized (BALB/c x C57BL/6N)F1 oocytes, and UGT1A1*28 founder mice from line 1, carrying 6 copies of the transgene on Chromosome 15, were bred to C57BL/6NJ mice for at least 5 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6NJ mice (Stock No. 005304) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 005304 C57BL/6NJ | (approximate) | |
| Considerations for Choosing Controls | ||
Phenotype
Phenotype Information
View Related Disease (OMIM) Terms
Related Disease (OMIM) Terms provided by MGI
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. Crigler-Najjar Syndrome, Type I (UGT1A1)
Crigler-Najjar Syndrome, Type II (UGT1A1)
Gilbert Syndrome (UGT1A1)
Hyperbilirubinemia, Transient Familial Neonatal; HBLRTFN (UGT1A1)
This mouse can be used to support research in many areas including:
Hematological Research
Jaundice
Internal/Organ Research
Liver Defects
Research Tools
Toxicology Research
drug metabolism
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(UGT1A1*28)1Rhtu | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Robert Tukey | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Tg(UGT1A1*28)Ugt1; | ||
| Mutation Made By | Robert Tukey, University of California San Diego | ||
| Strain of Origin | (BALB/c x C57BL/6N)F1 | ||
| Expressed Gene | UGT1A1, UDP glucuronosyltransferase 1 family, polypeptide A1, human | ||
| Promoter | UGT1A1, UDP glucuronosyltransferase 1 family, polypeptide A1, human | ||
| Molecular Note | A transgene derived from a human bacterial artificial chromosome (BAC) library, containing the entire human UGT1 (UDP glucuronosyltransferase 1) complex, was modified by targeted mutation of the UGT1 polypeptide A1, UGT1A1, promoter to harbor the UGT1A1*28 mutation associated with hepatic dysfunction found in Gilbert's syndrome. Specifically, this mutation inserts an extra TA in the TATA box sequence of the UGTA1A promoter. Five founders were generated. Founder mice from line 1 carry 6 copies of the transgene on Chromosome 15. [MGI Ref ID J:102898] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(UGT1A1*28), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Chen S; Beaton D; Nguyen N; Senekeo-Effenberger K; Brace-Sinnokrak E; Argikar U; Remmel RP; Trottier J; Barbier O; Ritter JK; Tukey RH. 2005. Tissue-specific, inducible, and hormonal control of the human UDP-glucuronosyltransferase-1 (UGT1) locus. J Biol Chem 280(45):37547-57. [PubMed: 16155002] [MGI Ref ID J:102898]
Fujiwara R; Nguyen N; Chen S; Tukey RH. 2010. Developmental hyperbilirubinemia and CNS toxicity in mice humanized with the UDP glucuronosyltransferase 1 (UGT1) locus. Proc Natl Acad Sci U S A 107(11):5024-9. [PubMed: 20194756] [MGI Ref ID J:158749]
Additional References
Tg(UGT1A1*28)1Rhtu relatedYueh MF; Li T; Evans RM; Hammock B; Tukey RH. 2012. Triclocarban mediates induction of xenobiotic metabolism through activation of the constitutive androstane receptor and the estrogen receptor alpha. PLoS One 7(6):e37705. [PubMed: 22761658] [MGI Ref ID J:187925]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 005304 C57BL/6NJ | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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JAX® Mice, Products & Services Conditions of Use
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