Strain Name:

B6(Cg)-Irf8tm1.1Hm/J

Stock Number:

014175

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
This Irf8f floxed targeted mutation strain may be useful in generating conditional mutants for studying B cell differentiation, immunodeficiency (abnormal populations of B cells, T cells, granulocytes and macrophages), hematopoiesis, lymphoma and human chronic myeloid leukemia.

Description

Strain Information

Type Coisogenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemHomozygote x Homozygote         (Female x Male)   06-JUN-12
Specieslaboratory mouse
GenerationF?+F8 (08-JUL-13)
Generation Definitions
 
Donating Investigator Herbert C. Morse III,   Laboratory of Immunopathology, NIAID, NI

Description
These mice possess loxP sites on either side of exon 2, which encodes the DNA binding domain. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in B lymphocytes (see Stock No. 006785 for example), this mutant mouse strain may be useful in studies of B cell differentiation.

Development
A targeting vector containing an FRT site flanked PGK-Neo selection cassette was utilized in the construction of this mutant. loxP sites were inserted upstream and downstream of exon 2. This construct was electroporated into C57BL/6 derived embryonic stem (ES) cells (Ozgene). Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to transgenic mice (on the C57BL/6 genetic background) expressing FLP recombinase. Mice that retained the loxP site flanked exon 2 were then bred to C57BL/6 mice to remove the FLP transgene. Heterozygotes were crossed to generate homozygotes. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Irf8
018298   B6(Cg)-Irf8tm1.2Hm/J
018655   B6N(Cg)-Irf8tm1b(KOMP)Wtsi/J
View Strains carrying other alleles of Irf8     (2 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Immunodeficiency 32a; IMD32A   (IRF8)
Monocyte and Dendritic Cell Deficiency, Autosomal Recessive   (IRF8)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
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Cd19tm1(cre)Cgn/Cd19+ Irf8tm1.1Hm/Irf8tm1.1Hm

        involves: 129P2/OlaHsd * C57BL/6   (conditional)
  • immune system phenotype
  • *normal* immune system phenotype
    • spleen and lymph node sizes are normal   (MGI Ref ID J:168923)
    • mice exhibit normal T-dependent and T-independent antibody responses   (MGI Ref ID J:168923)
    • abnormal spleen marginal zone morphology
      • mice exhibit enlarged marginal zones surrounding white pulp follicles compared with control mice   (MGI Ref ID J:168923)
      • increased marginal zone B cell number
        • the frequency of AA4-B220+IgMhi CD19+ marginal zone B cells is increased compared to in control mice   (MGI Ref ID J:168923)
        • the absolute number of marginal zone B cells is increased compared to in control mice   (MGI Ref ID J:168923)
    • decreased follicular B cell number
      • the proportion of AA4-B220+IgMlow CD19+ follicular B cells is decreased compared to in control mice   (MGI Ref ID J:168923)
    • increased B cell number
      • the frequency of CD19+B220+ B cells is increased compared to in control mice   (MGI Ref ID J:168923)
      • the number of total peritoneal B cells is increased compared to in control mice   (MGI Ref ID J:168923)
      • increased B-2 B cell number
        • in the peritoneum   (MGI Ref ID J:168923)
      • increased follicular B cell number
        • the absolute number of follicular B cells is increased compared to in control mice   (MGI Ref ID J:168923)
      • increased marginal zone B cell number
        • the frequency of AA4-B220+IgMhi CD19+ marginal zone B cells is increased compared to in control mice   (MGI Ref ID J:168923)
        • the absolute number of marginal zone B cells is increased compared to in control mice   (MGI Ref ID J:168923)
      • increased transitional stage B cell number
        • the frequency of B220+AA4+ transitional B cells is increased compared to in control mice   (MGI Ref ID J:168923)
        • the absolute number of transitional B cells is increased compared to in control mice   (MGI Ref ID J:168923)
  • hematopoietic system phenotype
  • abnormal spleen marginal zone morphology
    • mice exhibit enlarged marginal zones surrounding white pulp follicles compared with control mice   (MGI Ref ID J:168923)
    • increased marginal zone B cell number
      • the frequency of AA4-B220+IgMhi CD19+ marginal zone B cells is increased compared to in control mice   (MGI Ref ID J:168923)
      • the absolute number of marginal zone B cells is increased compared to in control mice   (MGI Ref ID J:168923)
  • decreased follicular B cell number
    • the proportion of AA4-B220+IgMlow CD19+ follicular B cells is decreased compared to in control mice   (MGI Ref ID J:168923)
  • increased B cell number
    • the frequency of CD19+B220+ B cells is increased compared to in control mice   (MGI Ref ID J:168923)
    • the number of total peritoneal B cells is increased compared to in control mice   (MGI Ref ID J:168923)
    • increased B-2 B cell number
      • in the peritoneum   (MGI Ref ID J:168923)
    • increased follicular B cell number
      • the absolute number of follicular B cells is increased compared to in control mice   (MGI Ref ID J:168923)
    • increased marginal zone B cell number
      • the frequency of AA4-B220+IgMhi CD19+ marginal zone B cells is increased compared to in control mice   (MGI Ref ID J:168923)
      • the absolute number of marginal zone B cells is increased compared to in control mice   (MGI Ref ID J:168923)
    • increased transitional stage B cell number
      • the frequency of B220+AA4+ transitional B cells is increased compared to in control mice   (MGI Ref ID J:168923)
      • the absolute number of transitional B cells is increased compared to in control mice   (MGI Ref ID J:168923)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Chronic Myelogenous Leukemia
Cre-lox System
      loxP-flanked Sequences

Cell Biology Research
Signal Transduction

Developmental Biology Research
Internal/Organ Defects
      hematopoietic defects
Lymphoid Tissue Defects
      hematopoietic defects

Hematological Research
Hematopoietic Defects

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency
      B and T cell deficiency
      B cell defects
      B cell deficiency
      Macrophage defects
      T cell deficiency
      defects in humoral immune responses
      multiple immune defects
Immunodeficiency Associated with Other Defects
Intracellular Signaling Molecules
Lymphoid Tissue Defects
      B and T cell deficiency
      hematopoietic development

Internal/Organ Research
Lymphoid Tissue Defects
      B and T cell deficiency
      T cell deficiency
Thymus Defects
      B and T cell deficient

Research Tools
Cancer Research
      B cell deficiency
      Cre-lox System
      Leukemia
      T cell deficiency
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System
Immunology, Inflammation and Autoimmunity Research
      B and T cell deficiency
      B cell deficiency
      Macrophage Deficiency
      T cell deficiency

Virology Research
B and T Cell Deficiency

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Irf8tm1.1Hm
Allele Name targeted mutation 1.1, Herbert Morse III
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Irf8f;
Mutation Made By Herbert Morse III,   Laboratory of Immunopathology, NIAID, NI
Strain of OriginB6.Cg-Thy1
Gene Symbol and Name Irf8, interferon regulatory factor 8
Chromosome 8
Gene Common Name(s) AI893568; H-ICSBP; ICSBP; ICSBP1; IMD32A; IMD32B; IRF-8; Icsbp1; Myls; expressed sequence AI893568; interferon consensus sequence binding protein 1; myeloproliferative syndrome;
Molecular Note Exon 2 was floxed. The FRT-flanked neo cassette used for selection was removed by flp-mediated recombination. [MGI Ref ID J:168923]

Genotyping

Genotyping Information

Genotyping Protocols

Irf8tm1Hm, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Feng J; Wang H; Shin DM; Masiuk M; Qi CF; Morse HC 3rd. 2011. IFN regulatory factor 8 restricts the size of the marginal zone and follicular B cell pools. J Immunol 186(3):1458-66. [PubMed: 21178004]  [MGI Ref ID J:168923]

Additional References

Irf8tm1.1Hm related

Jiang DS; Wei X; Zhang XF; Liu Y; Zhang Y; Chen K; Gao L; Zhou H; Zhu XH; Liu PP; Bond Lau W; Ma X; Zou Y; Zhang XD; Fan GC; Li H. 2014. IRF8 suppresses pathological cardiac remodelling by inhibiting calcineurin signalling. Nat Commun 5:3303. [PubMed: 24526256]  [MGI Ref ID J:206811]

Ouyang X; Zhang R; Yang J; Li Q; Qin L; Zhu C; Liu J; Ning H; Shin MS; Gupta M; Qi CF; He JC; Lira SA; Morse HC 3rd; Ozato K; Mayer L; Xiong H. 2011. Transcription factor IRF8 directs a silencing programme for TH17 cell differentiation. Nat Commun 2:314. [PubMed: 21587231]  [MGI Ref ID J:205655]

Pathak S; Ma S; Shukla V; Lu R. 2013. A role for IRF8 in B cell anergy. J Immunol 191(12):6222-30. [PubMed: 24218455]  [MGI Ref ID J:207133]

Sjostrand M; Ambrosi A; Brauner S; Sullivan J; Malin S; Kuchroo VK; Espinosa A; Wahren-Herlenius M. 2013. Expression of the immune regulator tripartite-motif 21 is controlled by IFN regulatory factors. J Immunol 191(7):3753-63. [PubMed: 23975864]  [MGI Ref ID J:205843]

Yoshida Y; Yoshimi R; Yoshii H; Kim D; Dey A; Xiong H; Munasinghe J; Yazawa I; O'Donovan MJ; Maximova OA; Sharma S; Zhu J; Wang H; Morse HC 3rd; Ozato K. 2014. The transcription factor IRF8 activates integrin-mediated TGF-beta signaling and promotes neuroinflammation. Immunity 40(2):187-98. [PubMed: 24485804]  [MGI Ref ID J:209803]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.
Mating SystemHomozygote x Homozygote         (Female x Male)   06-JUN-12
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $239.00Female or MaleHomozygous for Irf8tm1.1Hm  
Price per Pair (US dollars $)Pair Genotype
$478.00Homozygous for Irf8tm1.1Hm x Homozygous for Irf8tm1.1Hm  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $310.70Female or MaleHomozygous for Irf8tm1.1Hm  
Price per Pair (US dollars $)Pair Genotype
$621.40Homozygous for Irf8tm1.1Hm x Homozygous for Irf8tm1.1Hm  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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