Description

Strain Information

Type Qtl; Type Congenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male)   20-JUL-11 Species laboratory mouse Generation N2F19 (26-JUL-11) Generation Definitions   Donating Investigator Dr. Ken Johnson,   JAX

Description
This strain is congenic for a segment of distal Chromosome 10 bearing the age related hearing loss 4 (ahl4) mutation from A/J, which is a point mutation in the Cs gene. The ABR threshold average at 6 months of age was reported to be of 37.7 at 16 kHz, and at 9 months of age of 60 at 8 kHz, and 65 at 16 kHz, each significantly higher than that of C57BL/6J or heterozygotes.

Development
F1 offspring of C57BL/6J-Chr 10^A/J/NaJ x C57BL/6J were backcrossed further to C57BL/6J and the N2 generation was genotyped for markers along the length of Chromosome 10. The congenic interval with the proximal marker rs3676616 and the distal marker D10Utsw1, which includes ahl4, was then fixed homozygous by sibling intercrossing and the strain has been maintained this way ever since.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying   Cs^ahl4-A/J allele

000646	A/J
004388	C57BL/6J-Chr 10A/J/NaJ

View Strains carrying   Cs^ahl4-A/J     (2 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Cs^ahl4-A/J/Cs^ahl4-A/J

        C57BL/6J-Chr 10^A/J/NaJ

• hearing/vestibular/ear phenotype
• impaired hearing
  • 16 kHz ABR thresholds average 30 dB higher than in C57BL/6J controls   (MGI Ref ID J:139222)
• increased or absent threshold for auditory brainstem response
  • relative to C57BL/6J controls, C57BL/6J-Chr 10/NaJ consomic mice have ABR thresholds only slightly higher at 3 months of age, but 30 dB higher at 6 months of age and 50 dB higher at 9 months of age   (MGI Ref ID J:139222)
• increased susceptibility to age-related hearing loss
  • the onset of hearing loss occurs earlier and progresses more rapidly than in C57BL/6J   (MGI Ref ID J:139222)

Genes & Alleles

Gene & Allele Information provided by MGI

Gene Symbol and Name		D10Utsw1, DNA segment, Chr 10, University of Texas Southwestern 1
Chromosome		10
Allele Symbol		Csahl4-A/J
Allele Name		A/J
Allele Type		QTL
Strain of Origin		A/J
Gene Symbol and Name		Cs, citrate synthase
Chromosome		10
Gene Common Name(s)		2610511A05Rik; 9030605P22Rik; BB234005; Cis; RIKEN cDNA 2610511A05 gene; RIKEN cDNA 9030605P22 gene; age related hearing loss 4; ahl4; expressed sequence BB234005;
Gene Symbol and Name		Cs, citrate synthase
Chromosome		10
Gene Common Name(s)		2610511A05Rik; 9030605P22Rik; BB234005; Cis; RIKEN cDNA 2610511A05 gene; RIKEN cDNA 9030605P22 gene; age related hearing loss 4; ahl4; expressed sequence BB234005;

Genotyping

Genotyping Information

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Johnson KR; Gagnon LH; Longo-Guess C; Kane KL. 2011. Association of a citrate synthase missense mutation with age-related hearing loss in A/J mice Neurobiol Aging :.  [MGI Ref ID J:173679]

Additional References

Cs^ahl4-A/J related

Johnson KR; Gagnon LH; Longo-Guess C; Kane KL. 2012. Association of a citrate synthase missense mutation with age-related hearing loss in A/J mice. Neurobiol Aging 33(8):1720-9. [PubMed: 21803452]  [MGI Ref ID J:188196]

Zheng QY; Ding D; Yu H; Salvi RJ; Johnson KR. 2008. A locus on distal chromosome 10 (ahl4) affecting age-related hearing loss in A/J mice. Neurobiol Aging :. [PubMed: 18280008]  [MGI Ref ID J:139222]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           A1

Colony Maintenance

Mating System	Homozygote x Homozygote         (Female x Male)   20-JUL-11

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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