Strain Name:

B6.Cg-Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng/J

Stock Number:

014546

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Availability:

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ChAT-ChR2-YFP BAC transgenic mice may be useful in optogenetic studies for rapid control of motor behavior by addition or removal of light, for ex vivo and in vivo studies of neural circuitry/connectivity following illumination, for fluorescent labeling of cholinergic neuronal populations, or for studying the consequences of overactive cholinergic signaling in information processing, memory, behavior and physical fitness.

Description

Strain Information

Former Names B6.Cg-Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng    (Changed: 25-JUN-13 )
B6.Cg-Tg(Chat-COP4*H134R/EYFP)6Gfng/J    (Changed: 24-JUN-13 )
Type Congenic; Mutant Strain; Transgenic;
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Mating SystemNoncarrier x Hemizygote         (Female x Male)   19-SEP-11
Mating SystemHemizygote x Noncarrier         (Female x Male)   19-SEP-11
Specieslaboratory mouse
GenerationN6+F1N1 (21-OCT-11)
Generation Definitions
 
Donating Investigator Guoping Feng,   Massachusetts Institute of Technology

Important Note
Because the vesicular acetylcholine transporter gene (Slc18a3 or VAChT) within the Chat locus on the BAC transgene was not disrupted, ChAT-mhChR2-YFP BAC transgenic mice also have increased VAChT expression in hippocampus and brainstem. VAChT overexpression results in increased cholinergic tone; the functional consequence of which is increased physical endurance but severe cognitive deficits in attention, working memory and spatial memory.

Description
Mice hemizygous for the ChAT-ChR2-YFP BAC transgene are viable and fertile (see note below), with expression of the mhChR2::YFP fusion protein directed to cholinergic neuronal populations by the mouse choline acetyltransferase (Chat or ChAT) promoter/enhancer regions on the BAC transgene. The mhChR2::YFP fusion protein is composed of a mammalian codon-optimized Chlamydomonas reinhardtii-derived channelrhodopsin-2 that was modified to harbor a gain-of-function H134R substitution (mhChR2; also called hChR2-H134R) fused in-frame with an enhanced yellow fluorescent protein (EYFP). The mhChR2 is designed to cause larger stationary photocurrents compared to ChR2. The bacterial opsins are retinal-binding proteins that provide light-dependent ion transport and sensory functions to a family of halophilic bacteria; and this mhChR2 functions as a blue light-driven cation channel that depolarizes the cell and causes action potentials. As such, illuminating mhChR2-expressing neurons with blue light (450-490 nm) leads to rapid and reversible photostimulation of action potential firing/neural activity in these cells.

The donating investigator reports that there may be some problem with survival of transgenic males (especially when born from female transgenic dams). The donating investigator reports that EYFP expression is visible by direct fluorescence (epifluorescence microscope). ChAT-mhChR2-YFP mice derived from founder line 6 (ChAT-ChR2-YFP line 6) exhibit strong EYFP expression in striatum and basal forebrain, trochlear nucleus, medial habenula, interpeduncular nucleus and various brainstem motor neuron nuclei. Lower EYFP expression is found in cortex, hippocampus, and other brain regions. ChAT co-staining shows precise co-localization with mhChR2-EYFP expression neurons; suggesting mhChR2-EYFP labeled neurons in this line are cholinergic neurons. High EYFP fluorescence is also observed in the ventral gray horn in transverse section of the spinal cord. The donating investigator also reports that these ChAT-mhChR2-YFP line 6 mice exhibit a similar expression pattern as ChAT-mhChR2-YFP line 5 (Stock No. 014545), but line 6 mice have brighter EYFP expression.

Because the vesicular acetylcholine transporter gene (Slc18a3 or VAChT) within the Chat locus on the BAC transgene was not disrupted, VAChT protein is increased more than 5-fold in hippocampus and more than 3-fold in brainstem of ChAT-ChR2-YFP line 6 mice. This VAChT overexpression results in increased cholinergic tone; the functional consequence of which is increased physical endurance but severe cognitive deficits in attention, working memory and spatial memory. ChAT-ChR2-EYFP line 6 mice also consume more food and water during the dark cycle compared to control mice. Compared to wildtype animals, ChAT-ChR2-EYFP line 6 mice show no changes in metabolic profile, locomotor activity in a novel environment, anxiety-like behavior, depression-like behavior, gross sensorimotor function, and ability to use cues to learn a task.

This optogenetic strain is one of many from the same transgene creator/donating investigator with light-inducible neurobiology applications; including Thy1-ChR2-YFP line 18 (Stock No. 007612), Thy1-ChR2-YFP line 9 (Stock No. 007615), Thy1-eNpHR-YFP line 2 (Stock No. 012332), Thy1-eNpHR-YFP line 4 (Stock No. 012334), Thy1-vChR1-YFP line 1 (Stock No. 012341), Thy1-vChR1-YFP line 4 (Stock No. 012344), Thy1-vChR1-YFP line 8 (Stock No. 012348), Thy1-mhChR2-YFP Line 20 (Stock No. 012350), Prv-mhChR2-YFP Line 15 (Stock No. 012355), ChAT-ChR2-YFP line 5 (Stock No. 014545), VGAT-ChR2-YFP line 8 (Stock No. 014548), and TpH2-ChR2-YFP line 5 (Stock No. 014555).

Development
The ChAT-mhChR2-YFP BAC transgene (or ChAT-ChR2-YFP BAC transgene) was designed in the laboratory of Dr. Guoping Feng (Massachusetts Institute of Technology). First, a channelrhodopsin-2 cDNA sequence derived from green alga Chlamydomonas reinhardtii was modified to harbor codons optimized for mammalian expression and a gain-of-function H134R substitution (CAC to CGC) designed to cause larger stationary photocurrents. This mhChR2 sequence (also called hChR2-H134R) was fused in-frame to the amino terminus of an enhanced yellow fluorescent protein sequence (EYFP) via a Not1 site with GCGGCCGCC linker sequence. This mhChR2::YFP fusion protein (also called hChR2-H134R-EYFP) sequence was inserted into the coding region of the mouse choline acetyltransferase (Chat or ChAT) locus on the RP23-246B12 bacterial artificial chromosome (BAC) via homologous recombination. This was designed to disable transcription from the Chat locus, and none of the other loci on the BAC were altered. The BAC also includes the vesicular acetylcholine transporter gene (Slc18a3 or VAChT) that, because it is located in the intron between the first and second exons of the Chat gene, is under the control of the Chat promoter. The resulting ~220 kb ChAT-mhChR2-YFP BAC transgene was injected into B6SJLF1 fertilized oocytes. Transgenic founders were bred with C57BL/6J to generate the ChAT-mhChR2-YFP line 6 colony (also called ChAT-ChR2-YFP line 6). The colony was backcrossed to C57BL/6J mice for a total of at least five generations prior to sending to The Jackson Laboratory Repository in 2011. Upon arrival, sperm was cryopreserved. To generate the living colony, an aliquot of the frozen sperm was used to fertilize oocytes from C57BL/6J female mice (Stock No. 000664). To maintain our live ChAT-ChR2-YFP line 6 colony, hemizygous females are bred with wildtype (noncarrier) males from the colony or with C57BL/6J inbred males. There are approximately 50 copies of the transgene present. Of note, the donating investigator may not have fixed the Y chromosome to the C57BL/6J genetic background during backcrossing.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
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012344   B6;SJL-Tg(Thy1-COP3/EYFP)4Gfng/J
012348   B6;SJL-Tg(Thy1-COP3/EYFP)8Gfng/J
012350   B6;SJL-Tg(Thy1-COP4*H134R/EYFP)20Gfng/J
008004   B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J
007610   B6;SJL-Tg(Thy1-cre/ERT2,-EYFP)VGfng/J
012332   B6;SJL-Tg(Thy1-hop/EYFP)2Gfng/J
012334   B6;SJL-Tg(Thy1-hop/EYFP)4Gfng/J
014555   B6;SJL-Tg(Tph2-COP4*H134R/EYFP)5Gfng/J
019013   B6N.129S6(Cg)-Gt(ROSA)26Sortm2(EGFP/cre)Alj/J
023537   B6N.129S6-Gt(ROSA)26Sortm1(CAG-tdTomato*,-EGFP*)Ees/J
018974   B6N.B6-Tg(Nr4a1-EGFP/cre)820Khog/J
018913   B6N.Cg-Tg(tetO-GFP,-lacZ)G3Rsp/J
016532   B6N.FVB(Cg)-Tg(CAG-rtTA3)4288Slowe/J
025401   B6SJL(Cg)-Tg(Thy1-COX8A/Dendra)57Gmnf/J
007880   B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J
007856   B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J
004190   C.129-Il4tm1Lky/J
005700   C.129P2-Cxcr6tm1Litt/J
017580   C.129S4(B6)-Ifngtm3.1Lky/J
015864   C.129S4(B6)-Il12btm1Lky/J
017353   C.129S4(B6)-Il13tm1(YFP/cre)Lky/J
006769   C.Cg-Foxp3tm2Tch/J
010545   C.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
004512   C.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
008591   C57BL/6-Ackr3tm1Litt/J
008374   C57BL/6-Foxp3tm1Flv/J
008517   C57BL/6-Gt(ROSA)26Sortm3(CAG-MIR17-92,-EGFP)Rsky/J
012343   C57BL/6-Gt(ROSA)26Sortm7(Pik3ca*,EGFP)Rsky/J
012352   C57BL/6-Gt(ROSA)26Sortm8(Map2k1*,EGFP)Rsky/J
012361   C57BL/6-Gt(ROSA)26Sortm9(Rac1*,EGFP)Rsky/J
010724   C57BL/6-Trim21tm1Hm/J
006567   C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ
003291   C57BL/6-Tg(CAG-EGFP)1Osb/J
005070   C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J
012943   C57BL/6-Tg(Ins2-luc/EGFP/TK)300Kauf/J
016617   C57BL/6-Tg(Nr4a1-EGFP/cre)820Khog/J
012890   C57BL/6-Tg(Scgb1a1-Il17f,GFP)1Cdon/J
004353   C57BL/6-Tg(UBC-GFP)30Scha/J
005706   C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J
006618   C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J
006362   C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J
009655   C57BL/6J-Tg(Dcx-DsRed)14Qlu/J
007857   C57BL/6J-Tg(Eno2-YFP/Cox8a)YRwb/J
007860   C57BL/6J-Tg(Eno2-YFP/Cox8a)ZRwb/J
007567   C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J
009593   C57BL/6J-Tg(Pomc-EGFP)1Low/J
003927   C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ
008234   CB6-Tg(CAG-EGFP/CETN2)3-4Jgg/J
007677   CB6-Tg(Gad1-EGFP)G42Zjh/J
007898   CBy.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
007075   CByJ.B6-Tg(CAG-EGFP)1Osb/J
007076   CByJ.B6-Tg(UBC-GFP)30Scha/J
010548   D1.FVB(Cg)-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
008450   FVB-Tg(CAG-luc,-GFP)L2G85Chco/J
003718   FVB-Tg(GadGFP)45704Swn/J
010947   FVB-Tg(Gstm5-EGFP)1Ilis/J
005515   FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
010588   FVB-Tg(Myh6/NFAT-luc)1Jmol/J
006421   FVB-Tg(Pomc1-hrGFP)1Lowl/J
005688   FVB-Tg(Rag2-EGFP)1Mnz/J
005125   FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J
006206   FVB.129S6-Gt(ROSA)26Sortm2(HIF1A/luc)Kael/J
012429   FVB.Cg-Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/J
003516   FVB.Cg-Tg(CAG-EGFP)B5Nagy/J
016573   FVB.Cg-Tg(SMN2)89Ahmb Smn1tm1Msd Tg(S100B-EGFP)1Wjt Tg(SMN2*delta7)4299Ahmb/J
007483   FVB.Cg-Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
008200   FVB/N-Tg(CAG-EGFP,-ALPP)2.6Ggc/J
009354   FVB/N-Tg(Dazl-EGFP)10Rarp/J
003257   FVB/N-Tg(GFAPGFP)14Mes/J
007800   FVB/N-Tg(Ins1-luc)VUPwrs/J
012370   FVB/NJ-Tg(Hspa1a-luc,-EGFP)2Chco/J
009618   NOD.129(B6)-Il12btm1Lky/JbsJ
013116   NOD.B6-Tg(Ins2-luc/EGFP/TK)300Kauf/J
013233   NOD.B6-Tg(Itgax-cre,-EGFP)4097Ach/J
006698   NOD.Cg-Il4tm1Lky/JbsJ
008173   NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ
009422   NOD.Cg-Tg(Itgax-Venus)1Mnz/QtngJ
005076   NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ
010542   NOD.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
008547   NOD.FVB-Tg(ITGAM-DTR/EGFP)34Lan/JdkJ
008549   NOD.FVB-Tg(Itgax-DTR/EGFP)57Lan/JdkJ
005082   NOD/ShiLt-Tg(ACTB-Ica1/EGFP)18Mdos/MdosJ
005328   NOD/ShiLt-Tg(Cd4-DsRed)4Lt/J
005334   NOD/ShiLt-Tg(Cd4-EGFP)1Lt/J
008694   NOD/ShiLt-Tg(Foxp3-EGFP/cre)1cJbs/J
005282   NOD/ShiLtJ-Tg(Ins1-EGFP/GH1)14Hara/HaraJ
012881   STOCK Ascl1tm1Reed/J
008666   STOCK Fmn1tm1Made/J
013731   STOCK Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle/J
006331   STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J
005130   STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
017922   STOCK Gt(ROSA)26Sortm10(ACTB-tdTomato)Luo/J
018903   STOCK Gt(ROSA)26Sortm2(EGFP/cre)Alj/J
007576   STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
024107   STOCK Gt(ROSA)26Sortm5(ACTB-tTA)Luo Igs7tm93.1(tetO-GCaMP6f)Hze/HzeJ
017912   STOCK Gt(ROSA)26Sortm6(ACTB-EGFP*,-tdTomato)Luo/J
017921   STOCK Gt(ROSA)26Sortm7(ACTB-EGFP*)Luo/J
017909   STOCK Gt(ROSA)26Sortm8(ACTB-EGFP*,-tTA2)Luo/J
008876   STOCK Hprttm11(Ple176-EGFP/cre)Ems/Mmjax
009349   STOCK Hprttm31(Ple67-EGFP)Ems/Mmjax
009594   STOCK Hprttm32(Ple112-EGFP)Ems/Mmjax
022976   STOCK Igs2tm1(ACTB-EGFP,-tdTomato)Zng/J
022977   STOCK Igs2tm2(ACTB-tdTomato,-EGFP)Zng/J
013749   STOCK Iis2tm1(ACTB-EGFP,-tdTomato)Luo/J
013751   STOCK Iis2tm2(ACTB-tdTomato,-EGFP)Luo/J
017932   STOCK Iis3tm1.1(ACTB-EGFP*)Luo/J
017923   STOCK Iis3tm2.1(ACTB-EGFP*,-tdTomato)Luo/J
021458   STOCK Iis5tm1(ACTB-tdTomato,-EGFP)Luo/J
021457   STOCK Iis5tm2.1(ACTB-EGFP,-tdTomato)Luo/J
021461   STOCK Iis6tm1.1(ACTB-tdTomato,-EFGP)Luo/J
021460   STOCK Iis6tm2.1(ACTB-EFGP,-tdTomato)Luo/J
004808   STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
004779   STOCK Mapttm1(EGFP)Klt/J
005692   STOCK Nphs1tm1Rkl/J
006741   STOCK Olfr160tm1(Olfr151)Mom Tg(Olfr151,taulacZ)BMom/MomJ
006678   STOCK Olfr160tm6Mom/MomJ
006669   STOCK Olfr17tm7Mom/MomJ
009061   STOCK Osr1tm1(EGFP/cre/ERT2)Amc/J
007879   STOCK Stx1atm2Sud/J
014581   STOCK Trpm8tm1Apat/J
010911   STOCK Wt1tm1(EGFP/cre)Wtp/J
005438   STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J
006850   STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
006876   STOCK Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe/J
003920   STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
005441   STOCK Tg(CAG-DsRed*MST)1Nagy/J
003773   STOCK Tg(CAG-ECFP)CK6Nagy/J
003115   STOCK Tg(CAG-EGFP)B5Nagy/J
003116   STOCK Tg(CAG-EGFP)D4Nagy/J
011106   STOCK Tg(CAG-GFP*)1Hadj/J
013754   STOCK Tg(CAG-KikGR)75Hadj/J
011107   STOCK Tg(CAG-Venus)1Hadj/J
005645   STOCK Tg(CAG-mRFP1)1F1Hadj/J
005105   STOCK Tg(Chx10-EGFP/cre,-ALPP)2Clc/J
018322   STOCK Tg(Cp-EGFP)25Gaia/ReyaJ
008241   STOCK Tg(Cspg4-DsRed.T1)1Akik/J
006334   STOCK Tg(Gad1-EGFP)94Agmo/J
006340   STOCK Tg(Gad1-EGFP)98Agmo/J
007896   STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
016252   STOCK Tg(Hoxb7-Venus*)17Cos/J
006784   STOCK Tg(Ins1-Cerulean)24Hara/J
006866   STOCK Tg(Ins1-DsRed*T4)32Hara/J
016921   STOCK Tg(Myh2-DsRed2)1Jrs/J
012477   STOCK Tg(Myh6*/tetO-GCaMP2)1Mik/J
016922   STOCK Tg(Myh7-CFP)1Jrs/J
008579   STOCK Tg(PSCA-EGFP)1Witt/J
012452   STOCK Tg(Rr5-GFP/cre)1Sapc/J
006570   STOCK Tg(SMN2)89Ahmb Smn1tm1Msd Tg(Hlxb9-GFP)1Tmj/J
009606   STOCK Tg(Six2-EGFP/cre)1Amc/J
003658   STOCK Tg(TIE2GFP)287Sato/J
013162   STOCK Tg(Thy1-Clomeleon)12Gjau/J
013163   STOCK Tg(Thy1-Clomeleon)13Gjau/J
007788   STOCK Tg(Thy1-EGFP)MJrs/J
012708   STOCK Tg(Thy1-cre/ERT2,-EYFP)HGfng/PyngJ
011108   STOCK Tg(Ttr-RFP)1Hadj/J
016981   STOCK Tg(Uchl1-HIST2H2BE/mCherry/EGFP*)FSout/J
006129   STOCK Tg(Zp3-EGFP)1Dean/J
003274   STOCK Tg(tetNZL)2Bjd/J
005104   STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J
005699   STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
017918   STOCK Tg(tetO-MAML1*/EGFP)2Akar/J
012345   STOCK Tg(tetO-tdTomato,-Syp/EGFP*)1.1Luo/J
View Fluorescent Protein Strains     (377 strains)

View Strains carrying other alleles of COP4     (14 strains)

View Strains carrying other alleles of Chat     (6 strains)

Strains carrying other alleles of YFP
005483   129-Tg(CAG-EYFP)7AC5Nagy/J
021011   B6(D2)-Tg(CAG-Brainbow1.0)2Eggn/J
021012   B6(D2)-Tg(CAG-Brainbow1.0)3Eggn/J
016959   B6.129(Cg)-Foxp3tm4(YFP/cre)Ayr/J
010818   B6.129-Ifnb1tm1Lky/J
006412   B6.129-Il12btm1Lky/J
017578   B6.129S4-Mcpt8tm1(cre)Lky/J
006148   B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J
007920   B6.Cg-Gt(ROSA)26Sortm2(CAG-EYFP)Hze/J
007903   B6.Cg-Gt(ROSA)26Sortm3(CAG-EYFP)Hze/J
014545   B6.Cg-Tg(Chat-COP4*H134R/EYFP,Slc18a3)5Gfng/J
008829   B6.Cg-Tg(Itgax-Venus)1Mnz/J
008299   B6.Cg-Tg(NEFL-EYFP/Nefh)40Gsn/J
008828   B6.Cg-Tg(Prdm1-EYFP)1Mnz/J
014548   B6.Cg-Tg(Slc32a1-COP4*H134R/EYFP)8Gfng/J
007901   B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J
007911   B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J
007921   B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J
007612   B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J
007615   B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J
013161   B6.Cg-Tg(Thy1-Clomeleon)1Gjau/J
005630   B6.Cg-Tg(Thy1-EYFP)15Jrs/J
003709   B6.Cg-Tg(Thy1-YFP)16Jrs/J
003782   B6.Cg-Tg(Thy1-YFP)HJrs/J
005627   B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J
007606   B6.Cg-Tg(Thy1-cre/ERT2,-EYFP)AGfng/J
013081   B6.FVB-Tg(Per1-Venus)33Obr/Mmjax
006716   B6;129P2-Olfr545tm4Mom/MomJ
008774   B6;129P2-Runx3tm1Litt/J
014539   B6;129S-Gt(ROSA)26Sortm39(CAG-hop/EYFP)Hze/J
008636   B6;C-Tg(Prnp-APP695*/EYFP)49Gsn/J
007910   B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J
014130   B6;CBA-Tg(Thy1-YFP)GJrs/GfngJ
005620   B6;D2-Tg(S100B-EYFP)1Wjt/J
012355   B6;SJL-Tg(Pvalb-COP4*H134R/EYFP)15Gfng/J
012341   B6;SJL-Tg(Thy1-COP3/EYFP)1Gfng/J
012344   B6;SJL-Tg(Thy1-COP3/EYFP)4Gfng/J
012348   B6;SJL-Tg(Thy1-COP3/EYFP)8Gfng/J
012350   B6;SJL-Tg(Thy1-COP4*H134R/EYFP)20Gfng/J
007610   B6;SJL-Tg(Thy1-cre/ERT2,-EYFP)VGfng/J
012332   B6;SJL-Tg(Thy1-hop/EYFP)2Gfng/J
012334   B6;SJL-Tg(Thy1-hop/EYFP)4Gfng/J
014555   B6;SJL-Tg(Tph2-COP4*H134R/EYFP)5Gfng/J
025114   B6N.Cg-Tg(Camk2a-P2rx2/YC3.1)21Khakh/J
007880   B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J
015864   C.129S4(B6)-Il12btm1Lky/J
017353   C.129S4(B6)-Il13tm1(YFP/cre)Lky/J
017582   C.129S4(B6)-Mcpt8tm1(cre)Lky/J
008830   C.Cg-Tg(Itgax-Venus)1Mnz/J
017978   C57BL/6-Tg(Slc17a6-COP4*H134R/EYFP)2Oki/J
006618   C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J
006362   C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J
007857   C57BL/6J-Tg(Eno2-YFP/Cox8a)YRwb/J
007860   C57BL/6J-Tg(Eno2-YFP/Cox8a)ZRwb/J
025018   D2.Cg-Gpnmb+Tg(Thy1-YFP)HJrs/SjJ
024705   D2.Cg-Tg(Thy1-YFP)HJrs/SjJ
025019   D2.Cg-Tg(Thy1-YFP/Syp)10Jrs/SjJ
021065   FVB(C)-Tg(tetO-Npc1/YFP)1Mps/J
018067   FVB-Tg(Prism)1849Htz/J
018071   FVB-Tg(Prism)1861Htz/J
018068   FVB-Tg(Prism)1989Htz/J
009618   NOD.129(B6)-Il12btm1Lky/JbsJ
009422   NOD.Cg-Tg(Itgax-Venus)1Mnz/QtngJ
005130   STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
017472   STOCK Tg(Acp5-CFP,Ibsp-YFP,Dmp1-RFP)1Pmay/J
011107   STOCK Tg(CAG-Venus)1Hadj/J
020942   STOCK Tg(Cp-HIST1H2BB/Venus)47Hadj/J
016252   STOCK Tg(Hoxb7-Venus*)17Cos/J
024964   STOCK Tg(Pcp2-COP4*H134R/EYFP)U126Isop/J
021226   STOCK Tg(Thy1-Brainbow3.1)18Jrs/J
021225   STOCK Tg(Thy1-Brainbow3.1)3Jrs/J
021227   STOCK Tg(Thy1-Brainbow3.2)7Jrs/J
013162   STOCK Tg(Thy1-Clomeleon)12Gjau/J
013163   STOCK Tg(Thy1-Clomeleon)13Gjau/J
012708   STOCK Tg(Thy1-cre/ERT2,-EYFP)HGfng/PyngJ
025194   STOCK Tg(Vmn1r206-Mapt/YFP)1Dlc/J
View Strains carrying other alleles of YFP     (76 strains)

Additional Web Information

Fluorescent Proteins/lacZ Systems

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng/0

        B6.Cg-Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng/J
  • behavior/neurological phenotype
  • abnormal motor learning
    • mice do not improve time spent or distance travelled on rotarod as compared to controls   (MGI Ref ID J:197166)
  • abnormal spatial learning
    • mice visit target hole less than controls in Barnes maze test   (MGI Ref ID J:197166)
    • mice show no preference for target quadrant in Morris water maze during the probe trial, although they perform similar to controls in acquisition phase   (MGI Ref ID J:197166)
  • abnormal spatial working memory
    • mice revisit arms of Y-maze more often than controls, with less spontaneous alternations   (MGI Ref ID J:197166)
  • impaired cued conditioning behavior
    • mice did not improve performance in the cued version of Morris water maze   (MGI Ref ID J:197166)
  • increased fluid intake
    • mice consume more food and water during the dark cycle than controls   (MGI Ref ID J:197166)
  • increased food intake
    • mice consume more food and water during the dark cycle than controls   (MGI Ref ID J:197166)
  • homeostasis/metabolism phenotype
  • enhanced exercise endurance
    • mice run almost twice as much on treadmill as controls   (MGI Ref ID J:197166)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Fluorescent protein expression in neural tissue
Optogenetic and Pharmacogenetic tools
      Channelrhodopsin expressing strains

Research Tools
Fluorescent Proteins
Genetics Research
      Tissue/Cell Markers
      Tissue/Cell Markers: neurons
Neurobiology Research
      cell marker
Sensorineural Research

Sensorineural Research

YFP related

Research Tools
Fluorescent Proteins

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng
Allele Name transgene insertion 6, Guoping Feng
Allele Type Transgenic (Reporter)
Common Name(s) ChAT-ChR2-YFP line 6;
Mutation Made By Guoping Feng,   Massachusetts Institute of Technology
Strain of Origin(C57BL/6 x CBA)F1
Site of ExpressionEYFP expression in cholinergic neurons of the striatum, trochlear nucleus, medial habenula, interpeduncular nucleus and various brainstem motor neuron nuclei. Lower EYFP expression is found in cortex, hippocampus, and other brain regions.
Expressed Gene YFP, Yellow Fluorescent Protein, jellyfish
Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source.
Expressed Gene COP4, Channelrhodopsin, Chlamydomonas
Expressed Gene Slc18a3, solute carrier family 18 (vesicular monoamine), member 3, mouse, laboratory
Promoter Chat, choline acetyltransferase, mouse, laboratory
Molecular Note Transgene expression of the mhChR2::YFP fusion protein is directed to cholinergic neuronal populations by the mouse choline acetyltransferase (Chat or ChAT) promoter/enhancer regions on the BAC transgene. The mhChR2::YFP fusion protein is composed of a mammalian codon-optimized Chlamydomonas reinhardtii-derived channelrhodopsin-2 that was modified to harbor a gain-of-function H134R substitution (mhChR2; also called hChR2-H134R) fused in-frame with an enhanced yellow fluorescent protein (EYFP). The BAC used to generate the construct includes the vesicular acetylcholine transporter gene (Slc18a3 or VAChT). Slc18a3 is located in the intron between the first and second exons of the Chat gene and is under the control of the Chat promoter. VAChT protein is overexpressed in the hippocampus and brainstem. The mhChR2 is designed to cause larger stationary photocurrents compared to ChR2. The bacterial opsins are retinal-binding proteins that provide light-dependent ion transport and sensory functions to a family of halophilic bacteria; and this mhChR2 functions as a blue light-driven cation channel that depolarizes the cell and causes action potentials. As such, illuminating mhChR2-expressing neurons with blue light (450-490 nm) leads to rapid and reversible photostimulation of action potential firing/neural activity in these cells. EYFP expression is visible by direct fluorescence (epifluorescence microscope). ChAT-mhChR2-YFP mice derived from founder line 6 exhibit strong EYFP expression in striatum, trochlear nucleus, medial habenula, interpeduncular nucleus and various brainstem motor neuron nuclei. Lower EYFP expression is found in cortex, hippocampus, and other brain regions. ChAT co-staining shows precise co-localization with mhChR2-EYFP expression neurons; suggesting mhChR2-EYFP labeled neurons in this line are cholinergic neurons. High EYFP fluorescence is also observed in the ventral gray horn in transverse section of the spinal cord. The donating investigator also reports that these ChAT-mhChR2-YFP line 6 mice exhibit a similar expression pattern as ChAT-mhChR2-YFP line 5 (JAX Stock No. 014545), but line 6 mice have brighter EYFP expression. [MGI Ref ID J:171569] [MGI Ref ID J:197166]
 
 
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Chat-COP4*H134R/EYFP), Melt Curve Analysis
Tg(Chat-COP4*H134R/EYFP), Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Kolisnyk B; Guzman MS; Raulic S; Fan J; Magalhaes AC; Feng G; Gros R; Prado VF; Prado MA. 2013. ChAT-ChR2-EYFP mice have enhanced motor endurance but show deficits in attention and several additional cognitive domains J Neurosci 33(25):10427-10438. [PubMed: 23785154]  [MGI Ref ID J:197166]

Zhao S; Ting JT; Atallah HE; Qiu L; Tan J; Gloss B; Augustine GJ; Deisseroth K; Luo M; Graybiel AM; Feng G. 2011. Cell type-specific channelrhodpsin-2 transgenic mice for optogenetic dissection of neural circuitry function Nat Methods 8(9):745-755. [PubMed: 21985008]  [MGI Ref ID J:175525]

Additional References

Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng related

Feng G. 2011. Direct Data Submission 2011/05/16 MGI Direct Data Submission :.  [MGI Ref ID J:171569]

Hu F; Ren J; Zhang JE; Zhong W; Luo M. 2012. Natriuretic peptides block synaptic transmission by activating phosphodiesterase 2A and reducing presynaptic PKA activity. Proc Natl Acad Sci U S A 109(43):17681-6. [PubMed: 23045693]  [MGI Ref ID J:190372]

Ren J; Qin C; Hu F; Tan J; Qiu L; Zhao S; Feng G; Luo M. 2011. Habenula 'cholinergic' neurons co-release glutamate and acetylcholine and activate postsynaptic neurons via distinct transmission modes. Neuron 69(3):445-52. [PubMed: 21315256]  [MGI Ref ID J:174748]

Rothermel M; Carey RM; Puche A; Shipley MT; Wachowiak M. 2014. Cholinergic inputs from Basal forebrain add an excitatory bias to odor coding in the olfactory bulb. J Neurosci 34(13):4654-64. [PubMed: 24672011]  [MGI Ref ID J:210450]

Ting JT; Feng G. 2013. Development of transgenic animals for optogenetic manipulation of mammalian nervous system function: Progress and prospects for behavioral neuroscience. Behav Brain Res :. [PubMed: 23473879]  [MGI Ref ID J:197546]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & HusbandryThe donating investigator reports that there may be some problem with survival of transgenic males (especially when born from female transgenic dams). When maintaining a live colony, hemizygous females may be bred with wildtype (noncarrier) males from the colony or with C57BL/6J inbred male mice (Stock No. 000664).
Mating SystemNoncarrier x Hemizygote         (Female x Male)   19-SEP-11
Hemizygote x Noncarrier         (Female x Male)   19-SEP-11

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $239.00Female or MaleHemizygous for Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng  
Price per Pair (US dollars $)Pair Genotype
$311.00Hemizygous for Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng x Noncarrier  
$311.00Noncarrier x Hemizygous for Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $310.70Female or MaleHemizygous for Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng  
Price per Pair (US dollars $)Pair Genotype
$404.30Hemizygous for Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng x Noncarrier  
$404.30Noncarrier x Hemizygous for Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Important Note

Because the vesicular acetylcholine transporter gene (Slc18a3 or VAChT) within the Chat locus on the BAC transgene was not disrupted, ChAT-mhChR2-YFP BAC transgenic mice also have increased VAChT expression in hippocampus and brainstem. VAChT overexpression results in increased cholinergic tone; the functional consequence of which is increased physical endurance but severe cognitive deficits in attention, working memory and spatial memory.

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See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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JAX® Mice, Products & Services Conditions of Use

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