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| These transgenic mice allow Tet-Off/Tet-On regulated expression of FasL. FasL overexpression leads to Fas/FasL receptor-mediated death-signaling pathway activation and results in cell apoptosis. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N12pN1
Generation DefinitionsDonating Investigator Monique E De Paepe, Women and Infants Hospital Description
Mice hemizygous for the (tetOp)7-FasL transgene (TetOp-FasL transgenic mice) are viable and fertile with no reported phenotypic abnormalities. The (tetOp)7-FasL transgene has the Tet response element (TRE or tetO) upstream of a murine Fas ligand (FasL) coding sequence. When bred with other mice expressing tetracycline-controlled transactivator protein (tTA) or reverse tetracycline-controlled transactivator protein (rtTA), FasL overexpression in the resulting double mutant offspring can be regulated with tetracycline or its analog doxycycline (dox). FasL overexpression leads to Fas/FasL receptor-mediated death-signaling pathway activation and results in cell apoptosis. The donating investigator reports that transgenic mice from founder line B (TetOp-FasL transgenic line B) exhibit very high FasL overexpression levels in the presence of rtTA and 0.01 mg/ml Dox (low FasL levels are achievable by titrating Dox even lower). The donating investigator reports that TetOp-FasL transgenic line B mice exhibit "none-to-very-very-minimal" transgenic FasL expression in the absence of tTA or rtTA+Dox.Mice harboring the (tetOp)7-FasL transgene allow Tet-Off/Tet-On expression of FasL, and are a tool for temporal regulation of apoptotic cell death in specific tissue/cell types (via inducible FasL overexpression and subsequent activation of the Fas/FasL receptor-mediated death-signaling pathway).
For example, when TetOp-FasL transgenic line B mice are bred to a strain expressing rtTA in developing/adult lung and respiratory epithelium (CCSP-rtTA; Stock No. 006222), the resulting double transgenic mice (CCSP-rtTA/[tetOp]7-FasL) allow dox-inducible FasL-overexpression in respiratory epithelium and are a model for neonatal lung injury and bronchopulmonary dysplasia (BPD). Following dox administration to pregnant or nursing dams, CCSP-rtTA/[tetOp]7-FasL neonatal mice exhibit increased alveolar epithelial apoptosis induced by FasL overexpression that results in BPD-like disrupted alveolar remodeling.Development
The (tetOp)7-FasL transgene was designed in the laboratory of Dr. Monique E. De Paepe (Women and Infants Hospital, Providence, Rhode Island) with a Tet response element (TRE) upstream of the 943 bp murine Fas ligand (FasL) coding sequence, all followed by a bovine growth hormone intron and polyA sequence. The Tet response element (TRE) contains seven copies of the 42-bp tet operator sequence (tetO) just upstream of a minimal CMV promoter. The 2.9 kbp (tetOp)7-FasL transgene was injected into fertilized FVB/N oocytes. Founder mice from line B were found to contain ~6 copies of the transgene and high levels of doxycycline-induced FasL mRNA up-regulation. These founder mice were bred with FVB/NTac wildtype mice for 12-13 generations prior to sending to The Jackson Laboratory Repository. Upon arrival, TetOp-FasL transgenic line B mice were bred to FVB/NJ inbred mice (Stock No. 001800) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Fasl
001021 B6Smn.C3-Faslgld/J 000784 C3H/HeJ-Faslgld/J 002932 CPt.C3-Faslgld/J 003499 NOD-Tg(Ins2-Fasl)24Ach 008223 NOD.C3(B6)-Faslgld /LwnJ View Strains carrying other alleles of Fasl (5 strains)
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
- Potential model based on transgenic expression of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. Lung Cancer (FASLG)
This mouse can be used to support research in many areas including:
Apoptosis Research
Endogenous Regulators
Cell Biology Research
Signal Transduction
Immunology, Inflammation and Autoimmunity Research
Intracellular Signaling Molecules
Neurobiology Research
Tet Expression System
tTA/rtTA Responsive Strains
Research Tools
Apoptosis Research
Cancer Research
Tetop Tet System
Cardiovascular Research
Tetop Tet System
Genetics Research
Mutagenesis and Transgenesis: Tetop Tet System
Neurobiology Research
Tetop Tet System
Reproductive Biology Research
Tetop Tet System
Tet Expression Systems
tTA/rtTA Responsive Strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(tetO-Fasl)BDepa | ||
|---|---|---|---|
| Allele Name | transgene insertion B, Monique De Paepe | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | (tetOp)7-FasL transgenic line B; TetOp-FasL transgenic line B; | ||
| Mutation Made By | Monique De Paepe, Women and Infants Hospital | ||
| Strain of Origin | FVB/N | ||
| Expressed Gene | Fasl, Fas ligand (TNF superfamily, member 6), mouse, laboratory | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | The (tetO)7-FasL transgene was designed with a Tet response element (TRE) upstream of the 943 bp murine Fas ligand (FasL) coding sequence, all followed by a bovine growth hormone intron and polyA sequence. The Tet response element (TRE) contains seven copies of the 42-bp tet operator sequence (tetO) just upstream of a minimal CMV promoter. Five founder lines (A-E) were established. Founder mice from line B were found with ~6 copies of the transgene and high levels of doxycycline-induced FasL mRNA up-regulation. [MGI Ref ID J:137382] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(tetO-Fasl)BDepa, Melt Curve Analysis
Tg(tetO-Fasl)BDepa, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
De Paepe ME; Gundavarapu S; Tantravahi U; Pepperell JR; Haley SA; Luks FI; Mao Q. 2008. Fas-ligand-induced apoptosis of respiratory epithelial cells causes disruption of postcanalicular alveolar development. Am J Pathol 173(1):42-56. [PubMed: 18535181] [MGI Ref ID J:137382]
De Paepe ME; Mao Q; Ghanta S; Hovanesian V; Padbury JF. 2011. Alveolar epithelial cell therapy with human cord blood-derived hematopoietic progenitor cells. Am J Pathol 178(3):1329-39. [PubMed: 21356383] [MGI Ref ID J:169681]
Additional References
Tg(tetO-Fasl)BDepa relatedde Paepe ME; Haley SA; Lacourse Z; Mao Q. 2010. Effects of Fas-ligand overexpression on alveolar type II cell growth kinetics in perinatal murine lungs. Pediatr Res 68(1):57-62. [PubMed: 20375852] [MGI Ref ID J:174046]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred to wildtype siblings or to FVB/NJ inbred mice (Stock No. 001800). The donating investigator has not tried to generate homozygous mice.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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