Strain Name:

B6.Cg-Thy1a Tg(TcraCWM5,TcrbCWM5)1807Wuth/J

Stock Number:

014550

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These Bd 1807 TCR transgenic mice feature a CD4+ T cell repertoire that are reactive to several types of dimorphic fungi that cause major systemic mycoses found in North America.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating Investigator Bruce Klein,   University of Wisconsin

Important Note
The donating investigator reports that, in their colony (October 2011), some Bd 1807 transgenic mice develop a lymphoproliferative disorder ("lymphoproliferative cancer"); estimating that lymphoproliferative disorder penetrance is 30% or less by four months and 50% or less by six months (noting that these are very conservative estimates; the percentage of healthy mice could be higher). As such, the donating investigator recommends breeding mice between 2-4 months of age, and continuously breed young mice for experiments and maintenance of the colony.

Description
These Bd 1807 TCR transgenic mice feature a CD4+ T cell repertoire expressing a T cell receptor (TCR) specific for a protective, native epitope/shared antigen of Blastomyces dermatitidis and Histoplasma capsulatum. Bd 1807 T cells also respond to the related dimorphic fungi Coccidioides posadasii, Paracoccidioides lutzii, and Paracoccidioides brasiliensis. Bd 1807 T cells express a balanced Th1/Th17 profile.
On adoptive transfer of Bd 1807 cells into wildtype mice, subcutaneous vaccination with B. dermatitidis, H. capsulatum or C. posadasii yeast results in activation and expansion of Bd 1807 cells in the skin draining lymph nodes. Activated Bd 1807 cells migrate to the site of vaccination (the skin) where they differentiate into Th1 and Th17 cells. Upon subsequent lethal experimental challenge, Bd 1807 cells migrate to the lung and confer vaccine-induced resistance/protection against experimental pulmonary blastomycosis, histoplasmosis, and coccidiomycosis.
The donating investigator reports that, in their colony (October 2011), some Bd 1807 transgenic mice develop a lymphoproliferative disorder ("lymphoproliferative cancer"). The donating investigator estimates lymphoproliferative disorder penetrance as 30% or less by four months and 50% or less by six months (noting that these are very conservative estimates; the percentage of healthy mice could be higher).
These Bd 1807 TCR transgenic mice may be useful in studying cellular mechanisms of CD4+ T cell immunity, memory, and protection from the dimorphic fungi that cause the major systemic mycoses of North America, as well as prolonged duration of fungal antigen presentation and adoptive transfer studies of T cell priming at the respiratory mucosa.

These Bd 1807 TCR transgenic mice are further modified with the Thy1.1 allele (Thy1a), rather than the alternate allele present in C57BL/6 inbred mice (as well as C57BL/10, DBA/2, and BALB/c inbred mice). Thus, cell populations derived from these Bd 1807 TCR transgenic mice can be distinguished from syngeneic host and other mice with the alternate allele via flow cytometry. The presence of Thy1a serves as a marker for tracking donor CD4+ T cells in vitro.

Development
The Blastomyces dermatitidis-specific TCR transgenic mouse line 1807 (Bd 1807) was created by Drs. Marcel Wuethrich and Bruce Klein (University of Wisconsin). To generate the T cell clones, crude antigen preparations from cell wall/membrane (CW/M) of Blastomyces dermatitidis yeast cells were injected into C57BL/6 mice. The CD4+ T cell clones ("CW/M 5") were found to produce high levels of IFN-γ, to exhibit absent/low levels of type II cytokines (IL-4/IL-10), and also to transfer significant protective immunity when infected with a lethal dose of B. dermatitidis. The identity of the T cell receptor (TCR) Vα and Vβ chains on the surface of the "CW/M 5" T cells was found to be Vα2 Jα49 (amino acid motif of the CDR3 region is AGGYQNFYFGKGSLTVIP) and Vβ1/D/Jβ1.1 (amino acid motif of the CDR3 region is SQEGTGG). These TCR sequences were cloned into TCR cassette expression vectors (pTα and pTβ), and the resulting constructs were co-microinjected into C57BL/6 oocytes. Founder mice from line 1807 were bred with C57BL/6J mice to establish the colony. Linkage PCR determined that the transgenes had co-integrated in a TCRβ-TCRα head-to-tail and TCRα-TCRβ tail-to-tail fashion. These Bd 1807 TCR transgenic mice were bred with C57BL/6-Thy1.1 congenic mice (Stock No. 000406 ; B6.PL-Thy1a/Cy). Bd 1807 TCR transgenic mice were made homozygous for the Thy1.1 allele, and then maintained by breeding transgenic males with C57BL/6-Thy1.1 congenic females mice for at least ten generations prior to sending to The Jackson Laboratory Repository. Upon arrival, transgenic males were bred with B6.PL-Thy1a/CyJ mice (Stock No. 000406) females to establish and maintain the colony.

Control Information

  Control
   000406 B6.PL-Thy1a/CyJ
 
  Considerations for Choosing Controls

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005895   B10.Cg-Thy1a H2d Tg(TcraCl1,TcrbCl1)1Shrm/J
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003147   B10.D2-Hc1 H2d H2-T18c/nSnJ-Tg(DO11.10)10Dlo/J
003199   B10.PL-H2u H2-T18a/(73NS)Sn-Tg(TCRA)B1Jg/J
002116   B6.129S2-Tcratm1Mom/J
022073   B6.Cg-Rag1tm1Mom Thy1a Tg(Tcra2C,Tcrb2C)1Dlo/J
008684   B6.Cg-Rag1tm1Mom Tyrp1B-w Tg(Tcra,Tcrb)9Rest/J
005023   B6.Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J
005655   B6.Cg-Tg(Tcra,Tcrb)3Ayr/J
008428   B6.Cg-Tg(Tcra,Tcrb)HRCAll/J
008429   B6.Cg-Tg(Tcra,Tcrb)HRVAll/J
008006   B6.Cg-Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr/J
004194   B6.Cg-Tg(TcraTcrb)425Cbn/J
005236   B6.Cg-Tg(TcraY1,TcrbY1)416Tev/J
004554   B6.NOD-(D17Mit21-D17Mit10) Tg(TCRaAI4)1Dvs/DvsJ
002115   B6;129S2-Tcratm1Mom/J
004694   B6;D2-Tg(TcrLCMV)327Sdz/JDvsJ
002408   B6;SJL-Tg(TcrAND)53Hed/J
007848   BXSB.129P2(Cg)-Tcratm1Mjo/TheoJ
021880   BXSB.B6-Tg(TcraTcrb)1100Mjb/DcrJ
004364   C.Cg-Tcratm1Mom Tcrbtm1Mom/J
003303   C.Cg-Tg(DO11.10)10Dlo/J
002045   C.SJL-Tcrac/SlkJ
002047   C.SJL-Tcrba Tcrac/SlkJ
014639   C57BL/6-Tg(Cd4-TcraDN32D3)1Aben/J
011005   C57BL/6-Tg(H2-Kb-Tcra,-Tcrb)P25Ktk/J
006912   C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J
003831   C57BL/6-Tg(TcraTcrb)1100Mjb/J
005307   CBy.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005922   CBy.Cg-Thy1a Tg(TcraCl1,TcrbCl1)1Shrm/J
005694   D1Lac.Cg-Tg(Tcra,Tcrb)24Efro/J
017314   NOD-Tg(TcraTcrb)2H6Lwn/J
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006436   NOD.Cg-(Gpi1-D7Mit346)C57BL/6J Tg(TcraAI4)1Dvs/DvsJ
026243   NOD.Cg-(rs4135590-rs13480186) H2k2Tg(ILK3mHEL)3Ccg Tg(TcrHEL3A9)1Mmd/SlsgJ
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004460   NOD.Cg-Tg(TcraBDC2.5,TcrbBDC2.5)1Doi/DoiJ
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006303   NOD.FVB-Tg(TcraBDC12-4.1)10Jos/GseJ
004334   NOD/ShiLt-Tg(TcraAI4)1Dvs
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002597   STOCK Tg(TcrHEL3A9)1Mmd/J
View Strains carrying other alleles of Tcra     (50 strains)

Strains carrying other alleles of Tcrb
005308   B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
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002761   B10.Cg-Tg(TcrAND)53Hed/J
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002118   B6.129P2-Tcrbtm1Mom/J
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008684   B6.Cg-Rag1tm1Mom Tyrp1B-w Tg(Tcra,Tcrb)9Rest/J
005023   B6.Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J
005655   B6.Cg-Tg(Tcra,Tcrb)3Ayr/J
008428   B6.Cg-Tg(Tcra,Tcrb)HRCAll/J
008429   B6.Cg-Tg(Tcra,Tcrb)HRVAll/J
008006   B6.Cg-Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr/J
004194   B6.Cg-Tg(TcraTcrb)425Cbn/J
005236   B6.Cg-Tg(TcraY1,TcrbY1)416Tev/J
008430   B6.Cg-Tg(Tcrb)HRBAll/J
004555   B6.NOD-(D17Mit21-D17Mit10) Tg(TCRbAI4)1Dvs/DvsJ
002121   B6;129P-Tcrbtm1Mom Tcrdtm1Mom/J
002117   B6;129P2-Tcrbtm1Mom/J
004694   B6;D2-Tg(TcrLCMV)327Sdz/JDvsJ
002408   B6;SJL-Tg(TcrAND)53Hed/J
021880   BXSB.B6-Tg(TcraTcrb)1100Mjb/DcrJ
004364   C.Cg-Tcratm1Mom Tcrbtm1Mom/J
003303   C.Cg-Tg(DO11.10)10Dlo/J
002047   C.SJL-Tcrba Tcrac/SlkJ
002046   C.SJL-Tcrba/SlkJ
011005   C57BL/6-Tg(H2-Kb-Tcra,-Tcrb)P25Ktk/J
006912   C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J
003831   C57BL/6-Tg(TcraTcrb)1100Mjb/J
003540   C57L/J-Tg(Tcrb)93Vbo/J
005307   CBy.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005922   CBy.Cg-Thy1a Tg(TcraCl1,TcrbCl1)1Shrm/J
007081   CByJ.129P2(B6)-Tcrbtm1Mom/J
005694   D1Lac.Cg-Tg(Tcra,Tcrb)24Efro/J
017314   NOD-Tg(TcraTcrb)2H6Lwn/J
023082   NOD.129P2(Cg)-Tcrbtm1Mom/MnkaJ
006437   NOD.Cg-(Gpi1-D7Mit346)C57BL/6J Tg(TcrbAI4)1Dvs/DvsJ
026243   NOD.Cg-(rs4135590-rs13480186) H2k2Tg(ILK3mHEL)3Ccg Tg(TcrHEL3A9)1Mmd/SlsgJ
026624   NOD.Cg-(rs6385855-rs13480186) H2k2Tg(ILK3mHEL)3Ccg Tg(TcrHEL3A9)1Mmd/SlsgJ
004257   NOD.Cg-Prkdcscid Tg(TcrLCMV)327Sdz/DvsJ
009377   NOD.Cg-Rag1tm1Mom Tg(TcraBDC12-4.1)10Jos Tg(TcrbBDC12-4.1)82Gse/J
024476   NOD.Cg-Stat4tm1Gru Thy1a Ifngr1tm1Agt Tg(TcraBDC2.5,TcrbBDC2.5)1Doi/LmbrJ
005686   NOD.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
004696   NOD.Cg-Tg(TcrLCMV)327Sdz/DvsJ
004460   NOD.Cg-Tg(TcraBDC2.5,TcrbBDC2.5)1Doi/DoiJ
010526   NOD.Cg-Tg(TcraTcrbNY4.1)1Pesa/DvsJ
005868   NOD.Cg-Tg(TcraTcrbNY8.3)1Pesa/DvsJ
006304   NOD.FVB-Tg(TcrbBDC12-4.1)82Gse/GseJ
004335   NOD/ShiLt-Tg(TcrbAI4)1Dvs
018030   SJL.Cg-Tg(TcraTcrbVP2)1Bkim/J
002597   STOCK Tg(TcrHEL3A9)1Mmd/J
View Strains carrying other alleles of Tcrb     (52 strains)

Strains carrying other alleles of Thy1
017798   B6.Cg-Mapttm1Hnd Tg(Thy1-MAPT*)3610Gds/Mmjax
009126   B6.Cg-Nos2tm1Lau Tg(Thy1-APPSwDutIowa)BWevn/Mmjax
008730   B6.Cg-Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/Mmjax
007901   B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J
007911   B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J
007921   B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J
003710   B6.Cg-Tg(Thy1-CFP)23Jrs/J
014131   B6.Cg-Tg(Thy1-CFP)IJrs/GfngJ
007940   B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J
007967   B6.Cg-Tg(Thy1-CFP/COX8A)S2Lich/J
012597   B6.Cg-Tg(Thy1-COL25A1)861Yfu/J
007612   B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J
007615   B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J
013161   B6.Cg-Tg(Thy1-Clomeleon)1Gjau/J
007919   B6.Cg-Tg(Thy1-EGFP)OJrs/GfngJ
005630   B6.Cg-Tg(Thy1-EYFP)15Jrs/J
009611   B6.Cg-Tg(Thy1-Nlgn1)6Hnes/J
009612   B6.Cg-Tg(Thy1-Nlgn2)6Hnes/J
021069   B6.Cg-Tg(Thy1-PA-GFP)5Rmpl/J
021070   B6.Cg-Tg(Thy1-PA-GFP)6Rmpl/J
003709   B6.Cg-Tg(Thy1-YFP)16Jrs/J
003782   B6.Cg-Tg(Thy1-YFP)HJrs/J
005627   B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J
007606   B6.Cg-Tg(Thy1-cre/ERT2,-EYFP)AGfng/J
004807   B6;129-Psen1tm1Mpm Tg(APPSwe,tauP301L)1Lfa/Mmjax
007910   B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J
011070   B6;CBA-Tg(Thy1-EGFP)SJrs/NdivJ
017892   B6;CBA-Tg(Thy1-GCaMP2.2c)8Gfng/J
017893   B6;CBA-Tg(Thy1-GCaMP3)6Gfng/J
014130   B6;CBA-Tg(Thy1-YFP)GJrs/GfngJ
014651   B6;CBA-Tg(Thy1-spH)21Vnmu/J
015814   B6;CBA-Tg(Thy1-spH)64Vnmu/FrkJ
012341   B6;SJL-Tg(Thy1-COP3/EYFP)1Gfng/J
012344   B6;SJL-Tg(Thy1-COP3/EYFP)4Gfng/J
012348   B6;SJL-Tg(Thy1-COP3/EYFP)8Gfng/J
012350   B6;SJL-Tg(Thy1-COP4*H134R/EYFP)20Gfng/J
008004   B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J
012836   B6;SJL-Tg(Thy1-TARDBP)4Singh/J
007610   B6;SJL-Tg(Thy1-cre/ERT2,-EYFP)VGfng/J
012332   B6;SJL-Tg(Thy1-hop/EYFP)2Gfng/J
012334   B6;SJL-Tg(Thy1-hop/EYFP)4Gfng/J
006554   B6SJL-Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/Mmjax
025401   B6SJL-Tg(Thy1-COX8A/Dendra)57Gmnf/J
017590   B6SJL-Tg(Thy1-DCTN1*G59S)M2Pcw/J
007880   B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J
007856   B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J
017589   B6SJL-Tg(Thy1-TARDBP*G298S)S97Pcw/J
024703   C3A.Cg-Pde6b+Tg(Thy1-CFP)23Jrs/SjJ
007027   C57BL/6-Tg(Thy1-APPSwDutIowa)BWevn/Mmjax
010800   C57BL/6-Tg(Thy1-PTGS2)300Kand/J
010703   C57BL/6-Tg(Thy1-PTGS2)303Kand/J
012769   C57BL/6-Tg(Thy1-Sncg)HvP36Putt/J
024339   C57BL/6J-Tg(Thy1-GCaMP6f)GP5.11Dkim/J
025393   C57BL/6J-Tg(Thy1-GCaMP6f)GP5.17Dkim/J
024276   C57BL/6J-Tg(Thy1-GCaMP6f)GP5.5Dkim/J
025776   C57BL/6J-Tg(Thy1-GCaMP6s)GP4.12Dkim/J
024275   C57BL/6J-Tg(Thy1-GCaMP6s)GP4.3Dkim/J
025533   C57BL/6N-Sncatm1Mjff Tg(Thy1-SNCA)15Mjff/J
016936   C57BL/6N-Tg(Thy1-SNCA)12Mjff/J
017682   C57BL/6N-Tg(Thy1-SNCA)15Mjff/J
024704   D2.Cg-Gpnmb+Tg(Thy1-CFP)23Jrs/SjJ
025018   D2.Cg-Gpnmb+Tg(Thy1-YFP)HJrs/SjJ
018671   D2.Cg-Tg(Thy1-CFP)23Jrs/SjJ
024705   D2.Cg-Tg(Thy1-YFP)HJrs/SjJ
025019   D2.Cg-Tg(Thy1-YFP/Syp)10Jrs/SjJ
008230   FVB(Cg)-Tg(Thy1-SOD1*G93A)T3Hgrd/J
006143   FVB/N-Tg(Thy1-cre)1Vln/J
021226   STOCK Tg(Thy1-Brainbow3.1)18Jrs/J
021225   STOCK Tg(Thy1-Brainbow3.1)3Jrs/J
021227   STOCK Tg(Thy1-Brainbow3.2)7Jrs/J
013162   STOCK Tg(Thy1-Clomeleon)12Gjau/J
013163   STOCK Tg(Thy1-Clomeleon)13Gjau/J
007788   STOCK Tg(Thy1-EGFP)MJrs/J
012708   STOCK Tg(Thy1-cre/ERT2,-EYFP)HGfng/PyngJ
View Strains carrying other alleles of Thy1     (74 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Genes Regulating Growth and Proliferation

Cell Biology Research
Genes Regulating Growth and Proliferation
Signal Transduction

Developmental Biology Research
Internal/Organ Defects
      Lymphoid Tissue Defects
Lymphoid Tissue Defects

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
      genes regulating susceptibility to infectious disease and endotoxin
Growth Factors/Receptors/Cytokines
Intracellular Signaling Molecules
Lymphoid Tissue Defects
Rearranged Antigen-Specific T Cell Receptor Transgenes
T Cell Receptor Signaling Defects
      B and T cell deficiency, xenograft/transplant host

Internal/Organ Research
Lymphoid Tissue Defects

Research Tools
Developmental Biology Research
      transplantation marker for embryonic and adult tissue
Genetics Research
      Tissue/Cell Markers
      Tissue/Cell Markers: transplantation marker for embryonic and adult tissue
Immunology, Inflammation and Autoimmunity Research
      T Cell Receptor Transgenics
      T cell specific surface marker
      genes regulating susceptibility to infectious disease and endotoxin
      production of T cell lines and hybridomas

Thy1a related

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Research Tools
Genetics Research
      Tissue/Cell Markers
      Tissue/Cell Markers: T cell specific surface marker
Immunology, Inflammation and Autoimmunity Research
      T cell specific surface marker

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(TcraCWM5,TcrbCWM5)1807Wuth
Allele Name transgene insertion 1807, Marcel Wuethrich
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) Bd 1807 TCR Tg; Bd1807; Blastomyces-specific TCR Tg 1807;
Mutation Made By Marcel Wuethrich,   University of Wisconsin
Strain of OriginC57BL/6
Expressed Gene Tcra, T cell receptor alpha chain, mouse, laboratory
Expressed Gene Tcrb, T cell receptor beta chain, mouse, laboratory
Promoter Tcra, T cell receptor alpha chain, mouse, laboratory
Promoter Tcrb, T cell receptor beta chain, mouse, laboratory
Molecular Note The CW/M 5 CD4+ T cell line is from C57BL/6 mice injected with cell wall/membrane (CW/M) of Blastomyces dermatitidis yeast cells. The identity of the T cell receptor (TCR) Valpha and Vbeta chains on the surface of the "CW/M 5" T cells was found to be Valpha2 Jalpha49 (amino acid motif of the CDR3 region is AGGYQNFYFGKGSLTVIP) and Vbeta1/D/Jbeta1.1 (amino acid motif of the CDR3 region is SQEGTGG). The TCR-alpha gene segment, containing the rearranged VJ region from 7 bp upstream of the start codon to 71 bp beyond the Jalpha49 coding region into the JC intron, was amplified from genomic DNA of clone #5. This fragment was cloned into the pTalpha cassette. In a similar manner, the TCR-beta gene segment containing the rearranged VDJ region from 16 bp upstreamof the start codon to 17 bp beyond the Jbeta1.1 coding regions in the JC intron, was cloned into the pTbeta cassette. The resulting constructs were co-microinjected into C57BL/6 oocytes. Linkage PCR determined that the transgenes in line 1807 had co-integrated in a TCRbeta-TCRalpha head-to-tail and TCRalpha-TCRbeta tail-to-tail fashion. [MGI Ref ID J:182207]
 
 
 
Allele Symbol Thy1a
Allele Name a variant
Allele Type Not Applicable
Common Name(s) Thy-1.1; Thy1.1; Thy1a; theta-AKR; thetaAKR;
Site of ExpressionThe Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells.
Gene Symbol and Name Thy1, thymus cell antigen 1, theta
Chromosome 9
Gene Common Name(s) CD7; CD90; T25; Thy 1.2; Thy-1; Thy-1.2; Thy1.1; Thy1.2; theta;
General Note

The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells. The allele Thy1a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains; the allele Thy1b determines an antigenic specificity, Thy-1.2, found in the C3HeB/Fe and many other strains (J:5243, J:5012, J:4469). The Thy1 antigen is probably present on all T lymphocytes and absent from all B lymphocytes, and it thus serves as a valuable T-cell marker (J:5243). It is very widely used in experiments designed to determine the distribution and function of T-cells. Thy1 specifies a T-cell surface glycoprotein, T25, with a molecular weight of 25 kDa (J:5707). The protein appears to be anchored in the cell membrane by a lipid that is either phosphotidylinositol or closely related to it (J:12016). Thy1 may function in the cell membrane as a signal transduction molecule (J:8333). The Thy1 locus, or possibly a gene closely linked to it, controls quantitative expression of a protein that isthe same size as Thy1 and is expressed on thymus and brain but not on lymph node and spleen cells (J:7900).

Molecular Note The allele Thy1a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains.

Genotyping

Genotyping Information

Genotyping Protocols

Tg(TcraCWM5)1807Wuth, Separated PCR
Tg(TcrbCWM5)1807Wuth, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Kouskoff V; Signorelli K; Benoist C; Mathis D. 1995. Cassette vectors directing expression of T cell receptor genes in transgenic mice. J Immunol Methods 180(2):273-80. [PubMed: 7714342]  [MGI Ref ID J:99452]

Wuthrich M; Ersland K; Pick-Jacobs JC; Gern BH; Frye CA; Sullivan TD; Brennan MB; Filutowicz HI; O'Brien K; Korthauer KD; Schultz-Cherry S; Klein BS. 2012. Limited model antigen expression by transgenic fungi induces disparate fates during differentiation of adoptively transferred T cell receptor transgenic CD4+ T cells: robust activation and proliferation with weak effector function during recall. Infect Immun 80(2):787-97. [PubMed: 22124658]  [MGI Ref ID J:180817]

Wuthrich M; Ersland K; Sullivan T; Galles K; Klein BS. 2012. Fungi Subvert Vaccine T Cell Priming at the Respiratory Mucosa by Preventing Chemokine-Induced Influx of Inflammatory Monocytes. Immunity :. [PubMed: 22483803]  [MGI Ref ID J:182207]

Wuthrich M; Filutowicz HI; Allen HL; Deepe GS; Klein BS. 2007. V beta1+ J beta1.1+/V alpha2+ J alpha49+ CD4+ T cells mediate resistance against infection with Blastomyces dermatitidis. Infect Immun 75(1):193-200. [PubMed: 17030569]  [MGI Ref ID J:182208]

Wuthrich M; Gern B; Hung CY; Ersland K; Rocco N; Pick-Jacobs J; Galles K; Filutowicz H; Warner T; Evans M; Cole G; Klein B. 2011. Vaccine-induced protection against 3 systemic mycoses endemic to North America requires Th17 cells in mice. J Clin Invest 121(2):554-68. [PubMed: 21206087]  [MGI Ref ID J:171835]

Wuthrich M; Hung CY; Gern BH; Pick-Jacobs JC; Galles KJ; Filutowicz HI; Cole GT; Klein BS. 2011. A TCR transgenic mouse reactive with multiple systemic dimorphic fungi. J Immunol 187(3):1421-31. [PubMed: 21705621]  [MGI Ref ID J:179190]

Additional References

Thy1a related

Azzi J; Skartsis N; Mounayar M; Magee CN; Batal I; Ting C; Moore R; Riella LV; Ohori S; Abdoli R; Smith B; Fiorina P; Heathcote D; Bakhos T; Ashton-Rickardt PG; Abdi R. 2013. Serine protease inhibitor 6 plays a critical role in protecting murine granzyme B-producing regulatory T cells. J Immunol 191(5):2319-27. [PubMed: 23913965]  [MGI Ref ID J:205809]

Beck PL; Li Y; Wong J; Chen CW; Keenan CM; Sharkey KA; McCafferty DM. 2007. Inducible nitric oxide synthase from bone marrow-derived cells plays a critical role in regulating colonic inflammation. Gastroenterology 132(5):1778-90. [PubMed: 17449036]  [MGI Ref ID J:128325]

Brinkman CC; Rouhani SJ; Srinivasan N; Engelhard VH. 2013. Peripheral tissue homing receptors enable T cell entry into lymph nodes and affect the anatomical distribution of memory cells. J Immunol 191(5):2412-25. [PubMed: 23926324]  [MGI Ref ID J:205788]

Chen TT; Li L; Chung DH; Allen CD; Torti SV; Torti FM; Cyster JG; Chen CY; Brodsky FM; Niemi EC; Nakamura MC; Seaman WE; Daws MR. 2005. TIM-2 is expressed on B cells and in liver and kidney and is a receptor for H-ferritin endocytosis. J Exp Med 202(7):955-65. [PubMed: 16203866]  [MGI Ref ID J:107466]

Cuda CM; Wan S; Sobel ES; Croker BP; Morel L. 2007. Murine lupus susceptibility locus Sle1a controls regulatory T cell number and function through multiple mechanisms. J Immunol 179(11):7439-47. [PubMed: 18025188]  [MGI Ref ID J:154964]

D'Eustachio P; Owens GC; Edelman GM; Cunningham BA. 1985. Chromosomal location of the gene encoding the neural cell adhesion molecule (N-CAM) in the mouse. Proc Natl Acad Sci U S A 82(22):7631-5. [PubMed: 3865183]  [MGI Ref ID J:8111]

Dewals B; Hoving JC; Horsnell WG; Brombacher F. 2010. Control of Schistosoma mansoni egg-induced inflammation by IL-4-responsive CD4(+)CD25(-)CD103(+)Foxp3(-) cells is IL-10-dependent. Eur J Immunol 40(10):2837-47. [PubMed: 20821727]  [MGI Ref ID J:165816]

Divangahi M; Desjardins D; Nunes-Alves C; Remold HG; Behar SM. 2010. Eicosanoid pathways regulate adaptive immunity to Mycobacterium tuberculosis. Nat Immunol 11(8):751-8. [PubMed: 20622882]  [MGI Ref ID J:162390]

Dolfi DV; Duttagupta PA; Boesteanu AC; Mueller YM; Oliai CH; Borowski AB; Katsikis PD. 2011. Dendritic cells and CD28 costimulation are required to sustain virus-specific CD8+ T cell responses during the effector phase in vivo. J Immunol 186(8):4599-608. [PubMed: 21389258]  [MGI Ref ID J:172460]

Ertelt JM; Buyukbasaran EZ; Jiang TT; Rowe JH; Xin L; Way SS. 2013. B7-1/B7-2 blockade overrides the activation of protective CD8 T cells stimulated in the absence of Foxp3+ regulatory T cells. J Leukoc Biol 94(2):367-76. [PubMed: 23744647]  [MGI Ref ID J:204423]

Fife BT; Griffin MD; Abbas AK; Locksley RM; Bluestone JA. 2006. Inhibition of T cell activation and autoimmune diabetes using a B cell surface-linked CTLA-4 agonist. J Clin Invest 116(8):2252-61. [PubMed: 16886063]  [MGI Ref ID J:113109]

Green MC; Sweet HO. 1975. [Hx - Hm - W.] Mouse News Lett 52:38.  [MGI Ref ID J:13675]

Huang W; Huang F; Kannan AK; Hu J; August A. 2014. ITK tunes IL-4-induced development of innate memory CD8+ T cells in a gammadelta T and invariant NKT cell-independent manner. J Leukoc Biol 96(1):55-63. [PubMed: 24620029]  [MGI Ref ID J:212002]

Inlay MA; Bhattacharya D; Sahoo D; Serwold T; Seita J; Karsunky H; Plevritis SK; Dill DL; Weissman IL. 2009. Ly6d marks the earliest stage of B-cell specification and identifies the branchpoint between B-cell and T-cell development. Genes Dev 23(20):2376-81. [PubMed: 19833765]  [MGI Ref ID J:154864]

Kelly LM; Pereira JP; Yi T; Xu Y; Cyster JG. 2011. EBI2 guides serial movements of activated B cells and ligand activity is detectable in lymphoid and nonlymphoid tissues. J Immunol 187(6):3026-32. [PubMed: 21844396]  [MGI Ref ID J:179238]

Klebanoff CA; Spencer SP; Torabi-Parizi P; Grainger JR; Roychoudhuri R; Ji Y; Sukumar M; Muranski P; Scott CD; Hall JA; Ferreyra GA; Leonardi AJ; Borman ZA; Wang J; Palmer DC; Wilhelm C; Cai R; Sun J; Napoli JL; Danner RL; Gattinoni L; Belkaid Y; RestifoNP. 2013. Retinoic acid controls the homeostasis of pre-cDC-derived splenic and intestinal dendritic cells. J Exp Med 210(10):1961-76. [PubMed: 23999499]  [MGI Ref ID J:203429]

Krieg C; Letourneau S; Pantaleo G; Boyman O. 2010. Improved IL-2 immunotherapy by selective stimulation of IL-2 receptors on lymphocytes and endothelial cells. Proc Natl Acad Sci U S A 107(26):11906-11. [PubMed: 20547866]  [MGI Ref ID J:161365]

Lee SY; Goverman JM. 2013. The influence of T cell Ig mucin-3 signaling on central nervous system autoimmune disease is determined by the effector function of the pathogenic T cells. J Immunol 190(10):4991-9. [PubMed: 23562810]  [MGI Ref ID J:202569]

Leiter EH. 1998. NOD Mice and Related Strains: Origins, Husbandry and Biology Introduction. In: NOD Mice and Related Strains: Research Applications in Diabetes, AIDS, Cancer, and Other Diseases. RG Landes, Austin.  [MGI Ref ID J:110093]

Mitchell JS; Burbach BJ; Srivastava R; Fife BT; Shimizu Y. 2013. Multistage T cell-dendritic cell interactions control optimal CD4 T cell activation through the ADAP-SKAP55-signaling module. J Immunol 191(5):2372-83. [PubMed: 23918975]  [MGI Ref ID J:205802]

Pauken KE; Jenkins MK; Azuma M; Fife BT. 2013. PD-1, but not PD-L1, expressed by islet-reactive CD4+ T cells suppresses infiltration of the pancreas during type 1 diabetes. Diabetes 62(8):2859-69. [PubMed: 23545706]  [MGI Ref ID J:208973]

REIF AE; ALLEN JM. 1964. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med 120:413-33. [PubMed: 14207060]  [MGI Ref ID J:24839]

Rabenstein H; Behrendt AC; Ellwart JW; Naumann R; Horsch M; Beckers J; Obst R. 2014. Differential kinetics of antigen dependency of CD4+ and CD8+ T cells. J Immunol 192(8):3507-17. [PubMed: 24639353]  [MGI Ref ID J:210002]

Ranheim EA; Tarbell KV; Krogsgaard M; Mallet-Designe V; Teyton L; McDevitt HO; Weissman IL. 2004. Selection of aberrant class II restricted CD8+ T cells in NOD mice expressing a glutamic acid decarboxylase (GAD)65-specific T cell receptor transgene. Autoimmunity 37(8):555-67. [PubMed: 15763918]  [MGI Ref ID J:128250]

Read S; Hogan TV; Zwar TD; Gleeson PA; Van Driel IR. 2007. Prevention of autoimmune gastritis in mice requires extra-thymic T-cell deletion and suppression by regulatory T cells. Gastroenterology 133(2):547-58. [PubMed: 17603058]  [MGI Ref ID J:128303]

Reif AE; Allen JM. 1966. Mouse thymic iso-antigens. Nature 209(22):521-3. [PubMed: 5919593]  [MGI Ref ID J:5012]

Rodeghero R; Cao Y; Olalekan SA; Iwakua Y; Glant TT; Finnegan A. 2013. Location of CD4+ T cell priming regulates the differentiation of Th1 and Th17 cells and their contribution to arthritis. J Immunol 190(11):5423-35. [PubMed: 23630349]  [MGI Ref ID J:204778]

Sercan O; Stoycheva D; Hammerling GJ; Arnold B; Schuler T. 2010. IFN-gamma receptor signaling regulates memory CD8+ T cell differentiation. J Immunol 184(6):2855-62. [PubMed: 20164422]  [MGI Ref ID J:160112]

Uhl M; Kepp O; Jusforgues-Saklani H; Vicencio JM; Kroemer G; Albert ML. 2009. Autophagy within the antigen donor cell facilitates efficient antigen cross-priming of virus-specific CD8+ T cells. Cell Death Differ 16(7):991-1005. [PubMed: 19229247]  [MGI Ref ID J:164191]

Voehringer D; Liang HE; Locksley RM. 2008. Homeostasis and effector function of lymphopenia-induced 'memory-like' T cells in constitutively T cell-depleted mice. J Immunol 180(7):4742-53. [PubMed: 18354198]  [MGI Ref ID J:133382]

Wang L; Jameson SC; Hogquist KA. 2009. Epidermal Langerhans cells are not required for UV-induced immunosuppression. J Immunol 183(9):5548-53. [PubMed: 19843938]  [MGI Ref ID J:156799]

Wuthrich M; Warner T; Klein BS. 2005. IL-12 is required for induction but not maintenance of protective, memory responses to Blastomyces dermatitidis: implications for vaccine development in immune-deficient hosts. J Immunol 175(8):5288-97. [PubMed: 16210634]  [MGI Ref ID J:119110]

Xiao Z; Mescher MF; Jameson SC. 2007. Detuning CD8 T cells: down-regulation of CD8 expression, tetramer binding, and response during CTL activation. J Exp Med 204(11):2667-77. [PubMed: 17954566]  [MGI Ref ID J:126126]

Ysebrant de Lendonck L; Tonon S; Nguyen M; Vandevenne P; Welsby I; Martinet V; Molle C; Charbonnier LM; Leo O; Goriely S. 2013. Interferon regulatory factor 3 controls interleukin-17 expression in CD8 T lymphocytes. Proc Natl Acad Sci U S A 110(34):E3189-97. [PubMed: 23918362]  [MGI Ref ID J:200677]

Yu Y; Cho HI; Wang D; Kaosaard K; Anasetti C; Celis E; Yu XZ. 2013. Adoptive Transfer of Tc1 or Tc17 Cells Elicits Antitumor Immunity against Established Melanoma through Distinct Mechanisms. J Immunol 190(4):1873-81. [PubMed: 23315072]  [MGI Ref ID J:193239]

Zaleski M; Klein J. 1974. Immune response of mice to Thy-1. 1 antigen: genetic control by alleles at the Ir-5 locus loosely linked to the H-2 complex. J Immunol 113(4):1170-7. [PubMed: 4606643]  [MGI Ref ID J:5487]

Zaleski MB. 1975. Immune response of mice to the Thy-1.1 antigen: effect of the Ir-5 alleles studies in 129/J and B10.129(6M) mice Immunogenetics 2:241-8.  [MGI Ref ID J:30773]

Zecher D; Li Q; Oberbarnscheidt MH; Demetris AJ; Shlomchik WD; Rothstein DM; Lakkis FG. 2010. NK cells delay allograft rejection in lymphopenic hosts by downregulating the homeostatic proliferation of CD8+ T cells. J Immunol 184(12):6649-57. [PubMed: 20483732]  [MGI Ref ID J:161405]

Tg(TcraCWM5,TcrbCWM5)1807Wuth related

Wang H; LeBert V; Hung CY; Galles K; Saijo S; Lin X; Cole GT; Klein BS; Wuthrich M. 2014. C-type lectin receptors differentially induce th17 cells and vaccine immunity to the endemic mycosis of North America. J Immunol 192(3):1107-19. [PubMed: 24391211]  [MGI Ref ID J:207298]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, young males homozygous for the Thy1.1 allele (Thy1a) and hemizygous for the Bd 1807 transgene are bred with female B6.PL-Thy1a/CyJ mice (Stock No. 000406).

The donating investigator reports that, in their colony (October 2011), some Bd 1807 transgenic mice develop a lymphoproliferative disorder ("lymphoproliferative cancer"); estimating that lymphoproliferative disorder penetrance is 30% or less by four months and 50% or less by six months (noting that these are very conservative estimates; the percentage of healthy mice could be higher). As such, the donating investigator recommends breeding mice between 2-4 months of age, and continuously breed young mice for experiments and maintenance of the colony.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000406 B6.PL-Thy1a/CyJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Important Note

The donating investigator reports that, in their colony (October 2011), some Bd 1807 transgenic mice develop a lymphoproliferative disorder ("lymphoproliferative cancer"); estimating that lymphoproliferative disorder penetrance is 30% or less by four months and 50% or less by six months (noting that these are very conservative estimates; the percentage of healthy mice could be higher). As such, the donating investigator recommends breeding mice between 2-4 months of age, and continuously breed young mice for experiments and maintenance of the colony.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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