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| These Foxp3-eGFP/cre mice may be useful for fluorescently labeling Foxp3+ T-cells in studies of regulatory T cell function in autoimmunity and to remove loxP-flanked genomic DNA to allow Treg-specific activation/inactivation of conditional genes. | |||||||||||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 20-OCT-11 Species laboratory mouse Generation N8p+N1F1 (13-AUG-12)
Generation DefinitionsDonating Investigator Dr. Jeffrey A. Bluestone, University of California, San Francisco Description
These transgenic mice express an Enhanced Green Fluorescent Protein and codon optimized "humanized" cre recombinase fusion protein under the control of the mouse Foxp3 (forkhead box P3) promoter. Transgene expression is restricted to Foxp3 positive cells from the lymph nodes, spleen and thymus. GFP expression is restricted to FoxP3+ cells, which are mainly Cd4+Cd25 high. The Donating Investigator reports that Cre-mediated recombination and GFP expression is greater in this founder line (1a) than in the related founder line 1c (see Stock No. 008694) The Donating Investigator also reports that hemizygous pups may be smaller in size than wildtype littermates until to 4-6 weeks of age (after which they resume normal growth) and some hemizygous males may be infertile.Development
The 188 kb C57BL/6J mouse Bacterial Artificial Chromosome (BAC) RP23-143D8, containing the entire Foxp3 (forkhead box P3) gene was modified by inserting an Enhanced Green Fluorescent Protein (EGFP) fused to a codon optimized "humanized" cre recombinase coding sequence. This BAC transgene was injected into NOD/ShiLtJ fertilized oocytes and maintained on the same background. Founder line 1a was subsequently established. The mice were then backcrossed to the C57BL/6 for 7 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
016959 B6.129(Cg)-Foxp3tm4(YFP/cre)Ayr/J 004088 B6.Cg-Foxp3sf/J 018628 B6.Cg-Foxp3tm1Mal/J 019933 B6.Cg-Foxp3tm1Tch/J 006772 B6.Cg-Foxp3tm2Tch/J 016958 B6N.129(Cg)-Foxp3tm3Ayr/J 020654 C.129X1-Foxp3tm3Tch/J 021144 C.Cg-Foxp3tm1Tch/J 006769 C.Cg-Foxp3tm2Tch/J 008374 C57BL/6-Foxp3tm1Flv/J 006775 NOD.Cg-Foxp3sf/DoiJ 008694 NOD/ShiLt-Tg(Foxp3-EGFP/cre)1cJbs/J 016961 STOCK Foxp3tm9(EGFP/cre/ERT2)Ayr/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers: T cell specific surface marker
Immunology and Inflammation Research
T cell specific surface marker
cre relatedResearch Tools
Fluorescent Proteins
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Foxp3-EGFP/cre)1aJbs | ||
|---|---|---|---|
| Allele Name | transgene insertion 1a, Jeffrey Bluestone | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Mutation Made By | Dr. Jeffrey Bluestone, University of California, San Francisco | ||
| Strain of Origin | NOD/ShiLtJ | ||
| Site of Expression | Expression is detected in the lymph nodes and spleen. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Foxp3, forkhead box P3, mouse, laboratory | ||
| Driver Note | Foxp3 | ||
| Molecular Note | An EGFP-h-Cre cDNA (GFP fused to a codon-optimized, "humanized" cre recombinase sequence) was inserted directly downstream of the Foxp3 ATG start codon by homologous recombination in a 188-kb C57BL/6 BAC clone (RP23-143D8) containing the entire Foxp3 gene. The purified BAC was injected into pronuclei of nonobese, diabetic mouse embryos. Several founders were generated. GFP expression and lack of functional Foxp3 protein, as indicated by inability of the transgene to rescue Foxp3 null mice were analyzed and line 1 was established. Functional cre activity was assayed by breeding mice to Gt(ROSA)26Sortm1(EYFP)Cos reporter mice, and high specificity of recombination was detected in the lymph nodes and spleen. This is the first of two lines derived from founder 1. Cre mediated recombination and GFP expression is greater than in line 1c. [MGI Ref ID J:138808] | ||
| Gene Symbol and Name | Tg(Foxp3-EGFP/cre)1aJbs, transgene insertion 1a, Jeffrey Bluestone | ||
| Chromosome | UN | ||
Genotyping
Genotyping Information
Genotyping Protocols
Dicer1tm1Bdh, Standard PCR
Gt(Rosa)26Sortm1(EYFP)Cos, Standard PCR
gT(ROSA)26Sortm1(EYFP)Cos/J MCA SEP,Separated MCA
gT(ROSA)26Sortm1(EYFP)Cos/J MCA SEP, Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Zhou X; Jeker LT; Fife BT; Zhu S; Anderson MS; McManus MT; Bluestone JA. 2008. Selective miRNA disruption in T reg cells leads to uncontrolled autoimmunity. J Exp Med 205(9):1983-91. [PubMed: 18725525] [MGI Ref ID J:138808]
Additional References
Tg(Foxp3-EGFP/cre)1aJbs relatedBettini ML; Pan F; Bettini M; Finkelstein D; Rehg JE; Floess S; Bell BD; Ziegler SF; Huehn J; Pardoll DM; Vignali DA. 2012. Loss of epigenetic modification driven by the Foxp3 transcription factor leads to regulatory T cell insufficiency. Immunity 36(5):717-30. [PubMed: 22579476] [MGI Ref ID J:187320]
Gu AD; Wang Y; Lin L; Zhang SS; Wan YY. 2012. Requirements of transcription factor Smad-dependent and -independent TGF-beta signaling to control discrete T-cell functions. Proc Natl Acad Sci U S A :. [PubMed: 22219364] [MGI Ref ID J:179925]
Vanvalkenburgh J; Albu DI; Bapanpally C; Casanova S; Califano D; Jones DM; Ignatowicz L; Kawamoto S; Fagarasan S; Jenkins NA; Copeland NG; Liu P; Avram D. 2011. Critical role of Bcl11b in suppressor function of T regulatory cells and prevention of inflammatory bowel disease. J Exp Med 208(10):2069-81. [PubMed: 21875956] [MGI Ref ID J:177572]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, mice hemizygous for the transgene may be bred with mice wildtype (noncarrier) for the transgene. The Donating Investigator reports that hemizygous pups may be smaller in size than wildtype littermates until to 4-6 weeks of age (after which they catch up in size) and some hemizygous males seem to be infertile. Mating System Noncarrier x Hemizygote (Female x Male) 20-OCT-11 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
This strain is currently Under Development - Now Accepting Orders.
Estimated Available for Distribution Date:
27-MAY-13
Please note: You may now place orders for this strain although it is not yet ready for distribution. Estimated available for distribution dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for distribution depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain.
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(Foxp3-EGFP/cre)1aJbs
Price per Pair (US dollars $) Pair Genotype $296.00 Noncarrier x Hemizygous for Tg(Foxp3-EGFP/cre)1aJbs Standard Supply
Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(Foxp3-EGFP/cre)1aJbs
Price per Pair (US dollars $) Pair Genotype $384.80 Noncarrier x Hemizygous for Tg(Foxp3-EGFP/cre)1aJbs Standard Supply
Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.
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Standard Supply
Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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