Strain Name:

B6;129S4-Col1a1tm1(tetO-mCherry)Eggn/J

Stock Number:

014592

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These Col1a1-tetO-H2B-mcherry mutant mice contain a tet-responsive element (tetO) and a histone H2B-mCherry fusion protein, targeted to the collagen, type I, alpha 1 (Col1a1) locus. These mice may be useful for visualizing pluripotent cells.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
GenerationN4pN1
Generation Definitions
 
Donating Investigator Kevin Eggan,   Harvard University

Description
These Col1a1-tetO-H2B-mcherry mutant mice contain a tet-responsive element (tetO) and a histone H2B-mCherry fusion protein, targeted to the collagen, type I, alpha 1 (Col1a1) locus. Homozygous are viable, fertile, and normal is size. Animals have a subtle kink in the tail. When bred with another mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), expression of mCherry can be regulated with the tetracycline analog doxycycline (dox) in the resulting double mutant offspring.

Specifically, breeding Col1a1-tetO-H2B-mcherry mice with R26-rtTA mice (see Stock No. 006965) results in double mutant mice which express mCherry in embryonic stem (ES) cells, and differentiated trophoblast stem (TS) cells after administration of dox. These mice may be useful for visualizing pluripotent cells.

Development
A targeting vector was designed to insert frt-flanked PGK-Neo-pA into the 3' UTR of the collagen, type I, alpha 1 (Col1a1) locus. This construct was electroporated into (C57BL/6 x 129S4Sv/Jae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were identified. One of these ES cells clones, C10, was retargeted to insert an optimized form of reverse tetracycline controlled transactivator (rtTA-M2) followed by a β-globin intron and polyA signal downstream of the Gt(ROSA)26Sor promoter. Correctly targeted ES cells were identified. One of these ES cell clones, KH2, was selected and the frt-flanked PGK-Neo-pA in the 3' UTR of the Col1a1 locus was replaced by co-injection of a tetO-mCherry "flip-in plasmid" and a pCAG-Flpe plasmid. The tetO-mCherry "flip-in plasmid" contained a splice acceptor-double polyA sequence and the tetracycline responsive element (tetO) upstream of a histone H2B-mCherry fluorescent protein fusion protein.

The resulting ES cells, now targeted with rtTA-M2 in the Gt(ROSA)26Sor locus (Rosa26-rtTA) and tetO-mCherry in the Col1a1 locus (Col1a1-tetO-H2B-mCherry), were injected into recipient blastocysts. Chimeric mice were bred together to establish the double mutant colony. Double mutant mice were backcrossed onto a C57BL/6 background for at least 4 generations. Some animals were chosen, with the Col1a1-tetO-H2B-mCherry mutation and without the Rosa26-rtTA mutation, and sent to The Jackson Laboratory. These mutant mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Control Information

  Control
   101043 B6129SF1/J (approximate)
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Col1a1tm1(tetO-mCherry)Eggn allele
014602   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm1(tetO-mCherry)Eggn/J
View Strains carrying   Col1a1tm1(tetO-mCherry)Eggn     (1 strain)

View Strains carrying other alleles of Col1a1     (17 strains)

Additional Web Information

Tet Expression Systems

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Caffey Disease   (COL1A1)
Collagen, Type I, Alpha-1; COL1A1   (COL1A1)
Ehlers-Danlos Syndrome, Type I   (COL1A1)
Ehlers-Danlos Syndrome, Type VII, Autosomal Dominant   (COL1A1)
Osteogenesis Imperfecta, Type I   (COL1A1)
Osteogenesis Imperfecta, Type II   (COL1A1)
Osteogenesis Imperfecta, Type III   (COL1A1)
Osteogenesis Imperfecta, Type IV   (COL1A1)
Osteoporosis   (COL1A1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Col1a1tm1(tetO-mCherry)Eggn/Col1a1tm1(tetO-mCherry)Eggn

        involves: 129S4/SvJae * C57BL/6
  • normal phenotype
  • no abnormal phenotype detected   (MGI Ref ID J:167431)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Genetics Research
      Mutagenesis and Transgenesis: Tetop Tet System
      Tissue/Cell Markers
Reproductive Biology Research
      transplantation marker for embryonic and adult tissue

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Col1a1tm1(tetO-mCherry)Eggn
Allele Name targeted mutation 1, Kevin Eggan
Allele Type Targeted (Reporter)
Common Name(s) Col1a1TetoH2BCherry;
Mutation Made By Kevin Eggan,   Harvard University
Strain of Origin(C57BL/6 x 129S4/SvJae)F1
Site of ExpressionFollowing doxycycline administration, H2B-mCherry fusion protein is expressed in embryonic stem (ES) cells, and differentiated trophoblast stem (TS) cells.
Gene Symbol and Name Col1a1, collagen, type I, alpha 1
Chromosome 11
Gene Common Name(s) COLIA1; Col1a-1; Cola-1; Cola1; Moloney leukemia virus 13; Mov-13; OI4;
General Note The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae.
Molecular Note Using KH2 ES cells inserted a tetO-mCherry "flip-in plasmid" into the 3'UTR. The tetO-mCherry "flip-in plasmid" contained a splice acceptor-double polyA sequence and the tetracycline responsive element (tetO) upstream of a histone H2B minimal promoter-mCherry fluorescent protein fusion protein. [MGI Ref ID J:167431]

Genotyping

Genotyping Information

Genotyping Protocols

Col1a1 3'UTR assay2 (flip-in), Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Egli D; Rosains J; Birkhoff G; Eggan K. 2007. Developmental reprogramming after chromosome transfer into mitotic mouse zygotes. Nature 447(7145):679-85. [PubMed: 17554301]  [MGI Ref ID J:167431]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2085.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2710.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   101043 B6129SF1/J (approximate)
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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