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| The TVA transgene contains the human ubiquitin C (UBC) promoter driving expression of an avian specific retroviral receptor (TVA) gene derived from quail. These TVA mice may be useful for infection by retroviruses, rabies virus, and other viruses with ASLV glycoproteins. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6(SJL)-Tg(UBC-TVA)1Clc/J (Changed: 15-FEB-11 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N10pN1
Generation DefinitionsDonating Investigator Connie Cepko, Harvard Medical School, HHMI Description
The TVA transgene contains the human ubiquitin C (UBC) promoter driving expression of an avian specific retroviral receptor (TVA) gene derived from quail. Hemizygous mice are viable and fertile. UBC is a polyubiquitin precursor responsible for the regulation of cell signaling pathways upon conjugation with various proteins. Expression of TVA in these cells allows the binding of viruses with the envelope protein of avian sarcoma/leukosis virus subtype A (ASLV-A). These TVA mice may be useful for infection by retroviruses, rabies virus, and other viruses with ASLV glycoproteins.Development
The TVA transgene was designed with the human ubiquitin C (UBC) promoter driving expression of a loxP-flanked green fluorescent protein (GFP) and polyadenylation sequence, followed by an avian specific retroviral receptor (TVA) gene derived from quail. The transgene was microinjected into fertilized C57BL/6 X SJL/J F2 oocytes, and mice from founder line 1 were bred to C57BL/6J mice for at least ten generations. These mice were then bred to human β-actin-cre mice, on a C57BL/6 background, to remove the floxed-GFP-STOP cassette. Upon arrival at The Jackson Laboratory, the human β-actin-cre allele was bred away from. Transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of UBC
007179 129S.Cg-Tg(UBC-cre/ERT2)1Ejb/J 017614 B6(Cg)-Tyrc-2J Tg(UBC-mCherry)1Phbs/J 006259 B6.Cg-Pepcb Ptprca Tg(UBC-scFv)2Nemz/J 013115 B6.Cg-Rag1tm1Mom Tg(UBC-GFP)30Scha/J 015805 B6.Cg-Tg(UBC-GFP,-TVA)1Clc/J 015806 B6.Cg-Tg(UBC-GFP,-TVA)2Clc/J 015807 B6.Cg-Tg(UBC-GFP,-TVA)3Clc/J 008085 B6.Cg-Tg(UBC-cre/ERT2)1Ejb/J 007001 B6;129S-Tg(UBC-cre/ERT2)1Ejb/J 004353 C57BL/6-Tg(UBC-GFP)30Scha/J 007076 CByJ.B6-Tg(UBC-GFP)30Scha/J View Strains carrying other alleles of UBC (11 strains)
015805 B6.Cg-Tg(UBC-GFP,-TVA)1Clc/J 015806 B6.Cg-Tg(UBC-GFP,-TVA)2Clc/J 015807 B6.Cg-Tg(UBC-GFP,-TVA)3Clc/J 017617 FVB/N-Tg(Kdr-TVA)1026Ebp/J 011103 STOCK Olig2tm2(TVA,cre)Rth/J 003528 STOCK Tg(GFAP-TVA)5Hev/J 003529 STOCK Tg(NES-TVA)J12Ech/J
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Cell Biology Research
Signal Transduction
Research Tools
Cell Biology Research
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(UBC-TVA)1Clc | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Constance L Cepko | ||
| Allele Type | Transgenic (random, expressed) | ||
| Mutation Made By | Connie Cepko, Harvard Medical School, HHMI | ||
| Strain of Origin | (C57BL/6 x SJL/J)F2 | ||
| Expressed Gene | TVA, subgroup A avian leukosis virus receptor, quail | ||
| Promoter | UBC, ubiquitin C, human | ||
| Molecular Note | The TVA transgene was designed with the human ubiquitin C (UBC) promoter driving expression of a loxP-flanked green fluorescent protein (GFP) and polyadenylation sequence, followed by an avian specific retroviral receptor (TVA) gene derived from quail. [MGI Ref ID J:168308] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Cepko CL. 2011. Direct Data Submission 2011/02/15 MGI Direct Data Submission :. [MGI Ref ID J:168308]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony or to C57BL/6J inbred mice (Stock No. 000664).
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
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Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Terms of Use
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General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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