Strain Name:

NOD.129P2(B6)-P2rx7tm1Gab/DvsJ

Stock Number:

015809

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Cryopreserved - Ready for recovery

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Use Restrictions Apply, see Terms of Use
This NOD congenic strain contains a purinergic receptor P2X, ligand-gated ion channel, 7, P2rx7tm1Gab, targeted mutation. The P2x7 targeted mutation provides a tool to further dissect the interplay of ADP ribosylation and NAD-catabolizing enzymes and their effect on Tregs and iNKT involved in autoimmune type 1 diabetes.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain NOD/LtDvs
Donor Strain 129P2
GenerationN9F12p
Generation Definitions
 
Donating Investigator Christopher A. Gabel,   Pfizer Pharmaceuticals
Donating InvestigatorDr. David Serreze,   The Jackson Laboratory

Description
Mice that are homozygous for the targeted allele and commonly referred to as NOD.P2X7, are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. P2X7 located on chromosome 5 has been proposed as a candidate type 1 diabetes susceptibility gene.

On the C57BL/6 background, no gene product (mRNA or protein) is detected in cultured bone marrow mast cells or peritoneal macrophages. Samples of whole blood, as well as peritoneal macrophages, derived from mutant mice fail to produce extracellular interleukin 1 beta in response to lipopolysaccharide (LPS) and ATP treatment. Similarly, peritoneal lavage fluids from mutant animals that have been primed with LPS and subsequently challenged with ATP, are deficient in mature interleukin 1 beta, and at later time points, exhibit attenuated interleukin 6 levels when compared to fluids from similarly treated wildtype mice.

FACs analysis confirms P2X7 is absent on the surface of splenic T-cells of NOD.P2X7 homozygous mice. In addition, these splenic T-cells are resistant to NAD induced cell death. There are a greater number of splenic CD4+ iNKT-cells in NOD.P2X7 mice than NOD controls. There is no significant difference in diabetes onset between mutant and wildtype control mice. The presence of the P2X7 mutation combined with the Cd38 targeted mutation eliminates the accelerated diabetes seen in the NOD.Cd38 targeted mutation, Stock No. 004311 - NOD.129P2(B6)-Cd38tm1Lnd/LtJ (Chen et al, 2011).

This strain maybe useful to further study the role of P2rx7 in type 1 diabetes studies and provides a tool to further dissect the interplay of ADP ribosylation and NAD-catabolizing enzymes and their effect on Tregs and iNKT involved in autoimmune type 1 diabetes.

Development
A targeting vector containing a neomycin resistance gene driven by the mouse phosphoglycerate kinase promoter was used to disrupt the carboxyl-terminal coding region of the targeted gene. The construct was electroporated into 129P2/OlaHsd-derived E14TG2a embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the progeny of the chimeric animals were maintained on a C57BL/6 background (Stock No. 005576 - B6.129P2-P2rx7tm1Gab/J) prior to 9 backcross generations to NOD/ShiLtDvs. Using marker assisted technology, all known Idd loci were fixed to homozygosity. The congenic interval of this P2xr7 targeted mutation, located on chromosome 5, is less than 49Mb; with the proximal boundary located between D5Mit259 and P2xr7 and the distal boundary between D5Mit138 and D5Mit98. In 2011, the type 1 diabetes resource received this stock at generation N9F11.

Control Information

  Control
   Wild-type from the colony
   001976 NOD/ShiLtJ (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   P2rx7tm1Gab allele
005576   B6.129P2-P2rx7tm1Gab/J
View Strains carrying   P2rx7tm1Gab     (1 strain)

Strains carrying other alleles of P2rx7
000665   C57BL/10J
000664   C57BL/6J
000670   DBA/1J
000671   DBA/2J
View Strains carrying other alleles of P2rx7     (4 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM)

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
      Type 1 Diabetes
Growth Factors/Receptors/Cytokines

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol P2rx7tm1Gab
Allele Name targeted mutation 1, Christopher A Gabel
Allele Type Targeted (Null/Knockout)
Common Name(s) P2X7 KO; P2X7-; P2X7R-; P2X7RDelta506-532; Pfizer P2X7-;
Mutation Made By Patrick Gillespie,   Pfizer Pharmaceuticals
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14TG2a
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name P2rx7, purinergic receptor P2X, ligand-gated ion channel, 7
Chromosome 5
Gene Common Name(s) AI467586; P2X(7); P2X7; P2X7 receptor; P2X7R; expressed sequence AI467586;
Molecular Note Sequence encoding amino acids 506 through 532 was replaced by the insertion of a neomycin selection cassette. Transcript was undetected by Northern blot analysis of bone marrow mast cells isolated from homozygous mutant mice. Western blot analysis of homozygous mutant peritoneal macrophages showed an absence of normal and truncated protein. [MGI Ref ID J:66835]

Genotyping

Genotyping Information

Genotyping Protocols

P2rx7tm1Gab, Melt Curve Analysis
P2rx7tm1Gab, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Chen YG; Scheuplein F; Driver JP; Hewes AA; Reifsnyder PC; Leiter EH; Serreze DV. 2011. Testing the Role of P2X7 Receptors in the Development of Type 1 Diabetes in Nonobese Diabetic Mice. J Immunol :. [PubMed: 21357538]  [MGI Ref ID J:169661]

Solle M; Labasi J; Perregaux DG; Stam E; Petrushova N; Koller BH; Griffiths RJ; Gabel CA. 2001. Altered cytokine production in mice lacking P2X(7) receptors. J Biol Chem 276(1):125-32. [PubMed: 11016935]  [MGI Ref ID J:66835]

Additional References

P2rx7tm1Gab related

Allam R; Darisipudi MN; Rupanagudi KV; Lichtnekert J; Tschopp J; Anders HJ. 2011. Cutting Edge: Cyclic Polypeptide and Aminoglycoside Antibiotics Trigger IL-1{beta} Secretion by Activating the NLRP3 Inflammasome. J Immunol 186(5):2714-8. [PubMed: 21278344]  [MGI Ref ID J:169383]

Anselmi F; Hernandez VH; Crispino G; Seydel A; Ortolano S; Roper SD; Kessaris N; Richardson W; Rickheit G; Filippov MA; Monyer H; Mammano F. 2008. ATP release through connexin hemichannels and gap junction transfer of second messengers propagate Ca2+ signals across the inner ear. Proc Natl Acad Sci U S A 105(48):18770-5. [PubMed: 19047635]  [MGI Ref ID J:178055]

Apolloni S; Parisi C; Pesaresi MG; Rossi S; Carri MT; Cozzolino M; Volonte C; D'Ambrosi N. 2013. The NADPH oxidase pathway is dysregulated by the P2X7 receptor in the SOD1-G93A microglia model of amyotrophic lateral sclerosis. J Immunol 190(10):5187-95. [PubMed: 23589615]  [MGI Ref ID J:202552]

Arandjelovic S; McKenney KR; Leming SS; Mowen KA. 2012. ATP induces protein arginine deiminase 2-dependent citrullination in mast cells through the P2X7 purinergic receptor. J Immunol 189(8):4112-22. [PubMed: 22984079]  [MGI Ref ID J:190651]

Aswad F; Dennert G. 2006. P2X(7) receptor expression levels determine lethal effects of a purine based danger signal in T lymphocytes. Cell Immunol 243(1):58-65. [PubMed: 17286969]  [MGI Ref ID J:118682]

Aswad F; Kawamura H; Dennert G. 2005. High sensitivity of CD4+CD25+ regulatory T cells to extracellular metabolites nicotinamide adenine dinucleotide and ATP: a role for P2X7 receptors. J Immunol 175(5):3075-83. [PubMed: 16116196]  [MGI Ref ID J:113208]

Auger R; Motta I; Benihoud K; Ojcius DM; Kanellopoulos JM. 2005. A role for mitogen-activated protein kinase(Erk1/2) activation and non-selective pore formation in P2X7 receptor-mediated thymocyte death. J Biol Chem 280(30):28142-51. [PubMed: 15937334]  [MGI Ref ID J:100823]

Boucher AA; Arnold JC; Hunt GE; Spiro A; Spencer J; Brown C; McGregor IS; Bennett MR; Kassiou M. 2011. Resilience and reduced c-Fos expression in P2X7 receptor knockout mice exposed to repeated forced swim test. Neuroscience 189:170-7. [PubMed: 21664437]  [MGI Ref ID J:175649]

Bulanova E; Budagian V; Orinska Z; Koch-Nolte F; Haag F; Bulfone-Paus S. 2009. ATP induces P2X7 receptor-independent cytokine and chemokine expression through P2X1 and P2X3 receptors in murine mast cells. J Leukoc Biol 85(4):692-702. [PubMed: 19164130]  [MGI Ref ID J:146897]

Byrne SN; Beaugie C; O'Sullivan C; Leighton S; Halliday GM. 2011. The Immune-Modulating Cytokine and Endogenous Alarmin Interleukin-33 Is Upregulated in Skin Exposed to Inflammatory UVB Radiation. Am J Pathol 179(1):211-22. [PubMed: 21703403]  [MGI Ref ID J:173685]

Cao L; Li L; Lin D; Zuo Z. 2012. Isoflurane induces learning impairment that is mediated by interleukin 1beta in rodents. PLoS One 7(12):e51431. [PubMed: 23251531]  [MGI Ref ID J:195665]

Chatterjee S; Rana R; Corbett J; Kadiiska MB; Goldstein J; Mason RP. 2012. P2X7 receptor-NADPH oxidase axis mediates protein radical formation and Kupffer cell activation in carbon tetrachloride-mediated steatohepatitis in obese mice. Free Radic Biol Med 52(9):1666-79. [PubMed: 22343416]  [MGI Ref ID J:183259]

Chen L; Brosnan CF. 2006. Exacerbation of experimental autoimmune encephalomyelitis in P2X7R-/- mice: evidence for loss of apoptotic activity in lymphocytes. J Immunol 176(5):3115-26. [PubMed: 16493071]  [MGI Ref ID J:129413]

Chirila AM; Brown TE; Bishop RA; Bellono NW; Pucci FG; Kauer JA. 2014. Long-term potentiation of glycinergic synapses triggered by interleukin 1beta. Proc Natl Acad Sci U S A 111(22):8263-8. [PubMed: 24830427]  [MGI Ref ID J:211259]

Cowley SC; Hamilton E; Frelinger JA; Su J; Forman J; Elkins KL. 2005. CD4-CD8- T cells control intracellular bacterial infections both in vitro and in vivo. J Exp Med 202(2):309-19. [PubMed: 16027239]  [MGI Ref ID J:100513]

Csolle C; Baranyi M; Zsilla G; Kittel A; Goloncser F; Illes P; Papp E; Vizi ES; Sperlagh B. 2013. Neurochemical Changes in the Mouse Hippocampus Underlying the Antidepressant Effect of Genetic Deletion of P2X7 Receptors. PLoS One 8(6):e66547. [PubMed: 23805233]  [MGI Ref ID J:204206]

Daniel C; Wennhold K; Kim HJ; von Boehmer H. 2010. Enhancement of antigen-specific Treg vaccination in vivo. Proc Natl Acad Sci U S A 107(37):16246-51. [PubMed: 20805478]  [MGI Ref ID J:164370]

Darville T; Welter-Stahl L; Cruz C; Sater AA; Andrews CW Jr; Ojcius DM. 2007. Effect of the purinergic receptor P2X7 on Chlamydia infection in cervical epithelial cells and vaginally infected mice. J Immunol 179(6):3707-14. [PubMed: 17785807]  [MGI Ref ID J:152047]

Das S; Seth RK; Kumar A; Kadiiska MB; Michelotti G; Diehl AM; Chatterjee S. 2013. Purinergic receptor X7 is a key modulator of metabolic oxidative stress-mediated autophagy and inflammation in experimental nonalcoholic steatohepatitis. Am J Physiol Gastrointest Liver Physiol 305(12):G950-63. [PubMed: 24157968]  [MGI Ref ID J:210557]

Delarasse C; Auger R; Gonnord P; Fontaine B; Kanellopoulos JM. 2011. The purinergic receptor P2X7 triggers alpha-secretase-dependent processing of the amyloid precursor protein. J Biol Chem 286(4):2596-606. [PubMed: 21081501]  [MGI Ref ID J:168499]

Deplano S; Cook HT; Russell R; Franchi L; Schneiter S; Bhangal G; Unwin RJ; Pusey CD; Tam FW; Behmoaras J. 2013. P2X7 receptor-mediated Nlrp3-inflammasome activation is a genetic determinant of macrophage-dependent crescentic glomerulonephritis. J Leukoc Biol 93(1):127-34. [PubMed: 23089744]  [MGI Ref ID J:193771]

Deroide N; Li X; Lerouet D; Van Vre E; Baker L; Harrison J; Poittevin M; Masters L; Nih L; Margaill I; Iwakura Y; Ryffel B; Pocard M; Tedgui A; Kubis N; Mallat Z. 2013. MFGE8 inhibits inflammasome-induced IL-1beta production and limits postischemic cerebral injury. J Clin Invest 123(3):1176-81. [PubMed: 23454767]  [MGI Ref ID J:196376]

Diaz-Hernandez M; Diez-Zaera M; Sanchez-Nogueiro J; Gomez-Villafuertes R; Canals JM; Alberch J; Miras-Portugal MT; Lucas JJ. 2009. Altered P2X7-receptor level and function in mouse models of Huntington's disease and therapeutic efficacy of antagonist administration. FASEB J 23(6):1893-906. [PubMed: 19171786]  [MGI Ref ID J:150552]

Dorhoi A; Nouailles G; Jorg S; Hagens K; Heinemann E; Pradl L; Oberbeck-Muller D; Duque-Correa MA; Reece ST; Ruland J; Brosch R; Tschopp J; Gross O; Kaufmann SH. 2012. Activation of the NLRP3 inflammasome by Mycobacterium tuberculosis is uncoupled from susceptibility to active tuberculosis. Eur J Immunol 42(2):374-84. [PubMed: 22101787]  [MGI Ref ID J:179821]

Dostert C; Guarda G; Romero JF; Menu P; Gross O; Tardivel A; Suva ML; Stehle JC; Kopf M; Stamenkovic I; Corradin G; Tschopp J. 2009. Malarial hemozoin is a Nalp3 inflammasome activating danger signal. PLoS One 4(8):e6510. [PubMed: 19652710]  [MGI Ref ID J:152483]

Eltom S; Stevenson CS; Rastrick J; Dale N; Raemdonck K; Wong S; Catley MC; Belvisi MG; Birrell MA. 2011. P2X7 receptor and caspase 1 activation are central to airway inflammation observed after exposure to tobacco smoke. PLoS One 6(9):e24097. [PubMed: 21915284]  [MGI Ref ID J:177698]

Ewald SE; Chavarria-Smith J; Boothroyd JC. 2014. NLRP1 is an inflammasome sensor for Toxoplasma gondii. Infect Immun 82(1):460-8. [PubMed: 24218483]  [MGI Ref ID J:206165]

Franke H; Klimke K; Brinckmann U; Grosche J; Francke M; Sperlagh B; Reichenbach A; Liebert UG; Illes P. 2005. P2X(7) receptor-mRNA and -protein in the mouse retina; changes during retinal degeneration in BALBCrds mice. Neurochem Int 47(4):235-42. [PubMed: 15964665]  [MGI Ref ID J:103892]

Frascoli M; Marcandalli J; Schenk U; Grassi F. 2012. Purinergic P2X7 receptor drives T cell lineage choice and shapes peripheral gammadelta cells. J Immunol 189(1):174-80. [PubMed: 22649196]  [MGI Ref ID J:188945]

Furlan-Freguia C; Marchese P; Gruber A; Ruggeri ZM; Ruf W. 2011. P2X7 receptor signaling contributes to tissue factor-dependent thrombosis in mice. J Clin Invest 121(7):2932-44. [PubMed: 21670495]  [MGI Ref ID J:175647]

Garcia-Marcos M; Fontanils U; Aguirre A; Pochet S; Dehaye JP; Marino A. 2005. Role of sodium in mitochondrial membrane depolarization induced by P2X7 receptor activation in submandibular glands. FEBS Lett 579(24):5407-13. [PubMed: 16198349]  [MGI Ref ID J:102387]

Ghiringhelli F; Apetoh L; Tesniere A; Aymeric L; Ma Y; Ortiz C; Vermaelen K; Panaretakis T; Mignot G; Ullrich E; Perfettini JL; Schlemmer F; Tasdemir E; Uhl M; Genin P; Civas A; Ryffel B; Kanellopoulos J; Tschopp J; Andre F; Lidereau R; McLaughlin NM; Haynes NM; Smyth MJ; Kroemer G; Zitvogel L. 2009. Activation of the NLRP3 inflammasome in dendritic cells induces IL-1beta-dependent adaptive immunity against tumors. Nat Med 15(10):1170-8. [PubMed: 19767732]  [MGI Ref ID J:154302]

Glas R; Sauter NS; Schulthess FT; Shu L; Oberholzer J; Maedler K. 2009. Purinergic P2X7 receptors regulate secretion of interleukin-1 receptor antagonist and beta cell function and survival. Diabetologia 52(8):1579-88. [PubMed: 19396427]  [MGI Ref ID J:151265]

Goncalves RG; Gabrich L; Rosario A Jr; Takiya CM; Ferreira ML; Chiarini LB; Persechini PM; Coutinho-Silva R; Leite M Jr. 2006. The role of purinergic P2X7 receptors in the inflammation and fibrosis of unilateral ureteral obstruction in mice. Kidney Int 70(9):1599-606. [PubMed: 16969386]  [MGI Ref ID J:136485]

Gu BJ; Saunders BM; Petrou S; Wiley JS. 2011. P2X(7) is a scavenger receptor for apoptotic cells in the absence of its ligand, extracellular ATP. J Immunol 187(5):2365-75. [PubMed: 21821797]  [MGI Ref ID J:179265]

Haanes KA; Schwab A; Novak I. 2012. The P2X7 receptor supports both life and death in fibrogenic pancreatic stellate cells. PLoS One 7(12):e51164. [PubMed: 23284663]  [MGI Ref ID J:195637]

Habbas S; Ango F; Daniel H; Galante M. 2011. Purinergic signaling in the cerebellum: Bergmann glial cells express functional ionotropic P2X(7) receptors. Glia :. [PubMed: 21830236]  [MGI Ref ID J:176597]

He X; Mekasha S; Mavrogiorgos N; Fitzgerald KA; Lien E; Ingalls RR. 2010. Inflammation and fibrosis during Chlamydia pneumoniae infection is regulated by IL-1 and the NLRP3/ASC inflammasome. J Immunol 184(10):5743-54. [PubMed: 20393140]  [MGI Ref ID J:161000]

Heiss K; Janner N; Mahnss B; Schumacher V; Koch-Nolte F; Haag F; Mittrucker HW. 2008. High sensitivity of intestinal CD8+ T cells to nucleotides indicates P2X7 as a regulator for intestinal T cell responses. J Immunol 181(6):3861-9. [PubMed: 18768840]  [MGI Ref ID J:139109]

Hoque R; Sohail MA; Salhanick S; Malik AF; Ghani A; Robson SC; Mehal WZ. 2012. P2X7 receptor-mediated purinergic signaling promotes liver injury in acetaminophen hepatotoxicity in mice. Am J Physiol Gastrointest Liver Physiol 302(10):G1171-9. [PubMed: 22383490]  [MGI Ref ID J:190176]

Hubert S; Rissiek B; Klages K; Huehn J; Sparwasser T; Haag F; Koch-Nolte F; Boyer O; Seman M; Adriouch S. 2010. Extracellular NAD+ shapes the Foxp3+ regulatory T cell compartment through the ART2-P2X7 pathway. J Exp Med 207(12):2561-8. [PubMed: 20975043]  [MGI Ref ID J:176876]

Iyer SS; Pulskens WP; Sadler JJ; Butter LM; Teske GJ; Ulland TK; Eisenbarth SC; Florquin S; Flavell RA; Leemans JC; Sutterwala FS. 2009. Necrotic cells trigger a sterile inflammatory response through the Nlrp3 inflammasome. Proc Natl Acad Sci U S A 106(48):20388-93. [PubMed: 19918053]  [MGI Ref ID J:155576]

Jabs R; Matthias K; Grote A; Grauer M; Seifert G; Steinhauser C. 2007. Lack of P2X receptor mediated currents in astrocytes and GluR type glial cells of the hippocampal CA1 region. Glia 55(16):1648-55. [PubMed: 17849469]  [MGI Ref ID J:156297]

Ji X; Naito Y; Weng H; Endo K; Ma X; Iwai N. 2012. P2X7 deficiency attenuates hypertension and renal injury in deoxycorticosterone acetate-salt hypertension. Am J Physiol Renal Physiol 303(8):F1207-15. [PubMed: 22859404]  [MGI Ref ID J:188617]

Jin C; Frayssinet P; Pelker R; Cwirka D; Hu B; Vignery A; Eisenbarth SC; Flavell RA. 2011. NLRP3 inflammasome plays a critical role in the pathogenesis of hydroxyapatite-associated arthropathy. Proc Natl Acad Sci U S A 108(36):14867-72. [PubMed: 21856950]  [MGI Ref ID J:175225]

Joeckel LT; Wallich R; Martin P; Sanchez-Martinez D; Weber FC; Martin SF; Borner C; Pardo J; Froelich C; Simon MM. 2011. Mouse granzyme K has pro-inflammatory potential. Cell Death Differ 18(7):1112-9. [PubMed: 21311565]  [MGI Ref ID J:203106]

Jones CL; Weiss DS. 2011. TLR2 signaling contributes to rapid inflammasome activation during F. novicida infection. PLoS One 6(6):e20609. [PubMed: 21698237]  [MGI Ref ID J:174784]

Kahlenberg JM; Carmona-Rivera C; Smith CK; Kaplan MJ. 2013. Neutrophil Extracellular Trap-Associated Protein Activation of the NLRP3 Inflammasome Is Enhanced in Lupus Macrophages. J Immunol 190(3):1217-26. [PubMed: 23267025]  [MGI Ref ID J:192601]

Kawamura H; Aswad F; Minagawa M; Govindarajan S; Dennert G. 2006. P2X7 receptors regulate NKT cells in autoimmune hepatitis. J Immunol 176(4):2152-60. [PubMed: 16455971]  [MGI Ref ID J:129123]

Kawamura H; Kawamura T; Kanda Y; Kobayashi T; Abo T. 2012. Extracellular ATP-stimulated macrophages produce macrophage inflammatory protein-2 which is important for neutrophil migration. Immunology 136(4):448-58. [PubMed: 22564028]  [MGI Ref ID J:186262]

Ke HZ; Qi H; Weidema AF; Zhang Q; Panupinthu N; Crawford DT; Grasser WA; Paralkar VM; Li M; Audoly LP; Gabel CA; Jee WS; Dixon SJ; Sims SM; Thompson DD. 2003. Deletion of the P2X7 nucleotide receptor reveals its regulatory roles in bone formation and resorption. Mol Endocrinol 17(7):1356-67. [PubMed: 12677010]  [MGI Ref ID J:84145]

Kim JE; Kang TC. 2011. The P2X7 receptor-pannexin-1 complex decreases muscarinic acetylcholine receptor-mediated seizure susceptibility in mice. J Clin Invest 121(5):2037-47. [PubMed: 21505260]  [MGI Ref ID J:173942]

Kimbler DE; Shields J; Yanasak N; Vender JR; Dhandapani KM. 2012. Activation of P2X7 promotes cerebral edema and neurological injury after traumatic brain injury in mice. PLoS One 7(7):e41229. [PubMed: 22815977]  [MGI Ref ID J:189601]

Kistowska M; Gehrke S; Jankovic D; Kerl K; Fettelschoss A; Feldmeyer L; Fenini G; Kolios A; Navarini A; Ganceviciene R; Schauber J; Contassot E; French LE. 2014. IL-1beta Drives Inflammatory Responses to Propionibacterium acnes In Vitro and In Vivo. J Invest Dermatol 134(3):677-85. [PubMed: 24157462]  [MGI Ref ID J:206191]

Kouzaki H; Iijima K; Kobayashi T; O'Grady SM; Kita H. 2011. The Danger Signal, Extracellular ATP, Is a Sensor for an Airborne Allergen and Triggers IL-33 Release and Innate Th2-Type Responses. J Immunol 186(7):4375-87. [PubMed: 21357533]  [MGI Ref ID J:170695]

Kovarova M; Hesker PR; Jania L; Nguyen M; Snouwaert JN; Xiang Z; Lommatzsch SE; Huang MT; Ting JP; Koller BH. 2012. NLRP1-dependent pyroptosis leads to acute lung injury and morbidity in mice. J Immunol 189(4):2006-16. [PubMed: 22753929]  [MGI Ref ID J:189777]

Kuehnel MP; Reiss M; Anand PK; Treede I; Holzer D; Hoffmann E; Klapperstueck M; Steinberg TH; Markwardt F; Griffiths G. 2009. Sphingosine-1-phosphate receptors stimulate macrophage plasma-membrane actin assembly via ADP release, ATP synthesis and P2X7R activation. J Cell Sci 122(Pt 4):505-12. [PubMed: 19174470]  [MGI Ref ID J:146293]

Kuehnel MP; Rybin V; Anand PK; Anes E; Griffiths G. 2009. Lipids regulate P2X7-receptor-dependent actin assembly by phagosomes via ADP translocation and ATP synthesis in the phagosome lumen. J Cell Sci 122(Pt 4):499-504. [PubMed: 19174471]  [MGI Ref ID J:146292]

Kurashima Y; Amiya T; Fujisawa K; Shibata N; Suzuki Y; Kogure Y; Hashimoto E; Otsuka A; Kabashima K; Sato S; Sato T; Kubo M; Akira S; Miyake K; Kunisawa J; Kiyono H. 2014. The enzyme Cyp26b1 mediates inhibition of mast cell activation by fibroblasts to maintain skin-barrier homeostasis. Immunity 40(4):530-41. [PubMed: 24726878]  [MGI Ref ID J:209953]

Kurashima Y; Amiya T; Nochi T; Fujisawa K; Haraguchi T; Iba H; Tsutsui H; Sato S; Nakajima S; Iijima H; Kubo M; Kunisawa J; Kiyono H. 2012. Extracellular ATP mediates mast cell-dependent intestinal inflammation through P2X7 purinoceptors. Nat Commun 3:1034. [PubMed: 22948816]  [MGI Ref ID J:195470]

Labasi JM; Petrushova N; Donovan C; McCurdy S; Lira P; Payette MM; Brissette W; Wicks JR; Audoly L; Gabel CA. 2002. Absence of the P2X7 receptor alters leukocyte function and attenuates an inflammatory response. J Immunol 168(12):6436-45. [PubMed: 12055263]  [MGI Ref ID J:111397]

Labrousse VF; Costes L; Aubert A; Darnaudery M; Ferreira G; Amedee T; Laye S. 2009. Impaired interleukin-1beta and c-Fos expression in the hippocampus is associated with a spatial memory deficit in P2X(7) receptor-deficient mice. PLoS One 4(6):e6006. [PubMed: 19547756]  [MGI Ref ID J:150192]

Lammermann T; Afonso PV; Angermann BR; Wang JM; Kastenmuller W; Parent CA; Germain RN. 2013. Neutrophil swarms require LTB4 and integrins at sites of cell death in vivo. Nature 498(7454):371-5. [PubMed: 23708969]  [MGI Ref ID J:198733]

Le Gall SM; Legrand J; Benbijja M; Safya H; Benihoud K; Kanellopoulos JM; Bobe P. 2012. Loss of P2X7 receptor plasma membrane expression and function in pathogenic B220+ double-negative T lymphocytes of autoimmune MRL/lpr mice. PLoS One 7(12):e52161. [PubMed: 23284917]  [MGI Ref ID J:195619]

Le Stunff H; Auger R; Kanellopoulos J; Raymond MN. 2004. The Pro-451 to Leu polymorphism within the C-terminal tail of P2X7 receptor impairs cell death but not phospholipase D activation in murine thymocytes. J Biol Chem 279(17):16918-26. [PubMed: 14761980]  [MGI Ref ID J:89513]

Lee BH; Hwang DM; Palaniyar N; Grinstein S; Philpott DJ; Hu J. 2012. Activation of P2X(7) receptor by ATP plays an important role in regulating inflammatory responses during acute viral infection. PLoS One 7(4):e35812. [PubMed: 22558229]  [MGI Ref ID J:187281]

Lee GS; Subramanian N; Kim AI; Aksentijevich I; Goldbach-Mansky R; Sacks DB; Germain RN; Kastner DL; Chae JJ. 2012. The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP. Nature 492(7427):123-7. [PubMed: 23143333]  [MGI Ref ID J:192821]

Li J; Liu D; Ke HZ; Duncan RL; Turner CH. 2005. The P2X7 nucleotide receptor mediates skeletal mechanotransduction. J Biol Chem 280(52):42952-9. [PubMed: 16269410]  [MGI Ref ID J:105908]

Lischke T; Schumacher V; Wesolowski J; Hurwitz R; Haag F; Koch-Nolte F; Mittrucker HW. 2013. CD8-beta ADP-ribosylation affects CD8(+) T-cell function. Eur J Immunol 43(7):1828-38. [PubMed: 23575529]  [MGI Ref ID J:201025]

Lu R; Pan H; Shively JE. 2012. CEACAM1 negatively regulates IL-1beta production in LPS activated neutrophils by recruiting SHP-1 to a SYK-TLR4-CEACAM1 complex. PLoS Pathog 8(4):e1002597. [PubMed: 22496641]  [MGI Ref ID J:195394]

Mankus C; Chi C; Rich C; Ren R; Trinkaus-Randall V. 2012. The P2X(7) receptor regulates proteoglycan expression in the corneal stroma. Mol Vis 18:128-38. [PubMed: 22275804]  [MGI Ref ID J:191496]

Marin-Garcia P; Sanchez-Nogueiro J; Gomez-Villafuertes R; Leon D; Miras-Portugal MT. 2008. Synaptic terminals from mice midbrain exhibit functional P2X(7) receptor. Neuroscience 151(2):361-73. [PubMed: 18082965]  [MGI Ref ID J:130842]

Masin M; Young C; Lim K; Barnes SJ; Xu XJ; Marschall V; Brutkowski W; Mooney ER; Gorecki DC; Murrell-Lagnado R. 2012. Expression, assembly and function of novel C-terminal truncated variants of the mouse P2X7 receptor: re-evaluation of P2X7 knockouts. Br J Pharmacol 165(4):978-93. [PubMed: 21838754]  [MGI Ref ID J:194518]

Mayo C; Ren R; Rich C; Stepp MA; Trinkaus-Randall V. 2008. Regulation by P2X7: epithelial migration and stromal organization in the cornea. Invest Ophthalmol Vis Sci 49(10):4384-91. [PubMed: 18502993]  [MGI Ref ID J:141813]

Meuser-Batista M; Correa JR; Carvalho VF; de Carvalho Britto CF; da Cruz Moreira O; Batista MM; Soares MJ; Filho FA; E Silva PM; Lannes-Vieira J; Silva RC; Henriques-Pons A. 2011. Mast Cell Function and Death in Trypanosoma cruzi Infection. Am J Pathol 179(4):1894-904. [PubMed: 21819958]  [MGI Ref ID J:176304]

Mishra A; Chintagari NR; Guo Y; Weng T; Su L; Liu L. 2011. Purinergic P2X7 receptor regulates lung surfactant secretion in a paracrine manner. J Cell Sci 124(Pt 4):657-68. [PubMed: 21266468]  [MGI Ref ID J:182878]

Miyazaki T; Iwasawa M; Nakashima T; Mori S; Shigemoto K; Nakamura H; Katagiri H; Takayanagi H; Tanaka S. 2012. Intracellular and extracellular ATP coordinately regulate the inverse correlation between osteoclast survival and bone resorption. J Biol Chem 287(45):37808-23. [PubMed: 22988253]  [MGI Ref ID J:192471]

Munoz-Planillo R; Franchi L; Miller LS; Nunez G. 2009. A critical role for hemolysins and bacterial lipoproteins in Staphylococcus aureus-induced activation of the Nlrp3 inflammasome. J Immunol 183(6):3942-8. [PubMed: 19717510]  [MGI Ref ID J:152294]

Myers AJ; Eilertson B; Fulton SA; Flynn JL; Canaday DH. 2005. The purinergic P2X7 receptor is not required for control of pulmonary Mycobacterium tuberculosis infection. Infect Immun 73(5):3192-5. [PubMed: 15845532]  [MGI Ref ID J:97621]

Niessen F; Schaffner F; Furlan-Freguia C; Pawlinski R; Bhattacharjee G; Chun J; Derian CK; Andrade-Gordon P; Rosen H; Ruf W. 2008. Dendritic cell PAR1-S1P3 signalling couples coagulation and inflammation. Nature 452(7187):654-8. [PubMed: 18305483]  [MGI Ref ID J:133917]

Norton JT; Hayashi T; Crain B; Cho JS; Miller LS; Corr M; Carson DA. 2012. Cutting edge: nitrogen bisphosphonate-induced inflammation is dependent upon mast cells and IL-1. J Immunol 188(7):2977-80. [PubMed: 22387558]  [MGI Ref ID J:183090]

Notomi S; Hisatomi T; Kanemaru T; Takeda A; Ikeda Y; Enaida H; Kroemer G; Ishibashi T. 2011. Critical involvement of extracellular ATP acting on P2RX7 purinergic receptors in photoreceptor cell death. Am J Pathol 179(6):2798-809. [PubMed: 21983632]  [MGI Ref ID J:180087]

Notomi S; Hisatomi T; Murakami Y; Terasaki H; Sonoda S; Asato R; Takeda A; Ikeda Y; Enaida H; Sakamoto T; Ishibashi T. 2013. Dynamic increase in extracellular ATP accelerates photoreceptor cell apoptosis via ligation of P2RX7 in subretinal hemorrhage. PLoS One 8(1):e53338. [PubMed: 23308196]  [MGI Ref ID J:195812]

Novak I; Jans IM; Wohlfahrt L. 2010. Effect of P2X(7) receptor knockout on exocrine secretion of pancreas, salivary glands and lacrimal glands. J Physiol 588(Pt 18):3615-27. [PubMed: 20643770]  [MGI Ref ID J:176756]

Oliveira JF; Riedel T; Leichsenring A; Heine C; Franke H; Krugel U; Norenberg W; Illes P. 2011. Rodent cortical astroglia express in situ functional P2X7 receptors sensing pathologically high ATP concentrations. Cereb Cortex 21(4):806-20. [PubMed: 20739479]  [MGI Ref ID J:181824]

Panupinthu N; Rogers JT; Zhao L; Solano-Flores LP; Possmayer F; Sims SM; Dixon SJ. 2008. P2X7 receptors on osteoblasts couple to production of lysophosphatidic acid: a signaling axis promoting osteogenesis. J Cell Biol 181(5):859-71. [PubMed: 18519738]  [MGI Ref ID J:137037]

Panupinthu N; Zhao L; Possmayer F; Ke HZ; Sims SM; Dixon SJ. 2007. P2X7 nucleotide receptors mediate blebbing in osteoblasts through a pathway involving lysophosphatidic acid. J Biol Chem 282(5):3403-12. [PubMed: 17135244]  [MGI Ref ID J:120291]

Papp L; Vizi ES; Sperlagh B. 2004. Lack of ATP-evoked GABA and glutamate release in the hippocampus of P2X7 receptor-/- mice. Neuroreport 15(15):2387-91. [PubMed: 15640761]  [MGI Ref ID J:103703]

Qu Y; Franchi L; Nunez G; Dubyak GR. 2007. Nonclassical IL-1 beta secretion stimulated by P2X7 receptors is dependent on inflammasome activation and correlated with exosome release in murine macrophages. J Immunol 179(3):1913-25. [PubMed: 17641058]  [MGI Ref ID J:149937]

Ritter M; Gross O; Kays S; Ruland J; Nimmerjahn F; Saijo S; Tschopp J; Layland LE; Prazeres da Costa C. 2010. Schistosoma mansoni triggers Dectin-2, which activates the Nlrp3 inflammasome and alters adaptive immune responses. Proc Natl Acad Sci U S A 107(47):20459-64. [PubMed: 21059925]  [MGI Ref ID J:166590]

Sanchez-Nogueiro J; Marin-Garcia P; Miras-Portugal MT. 2005. Characterization of a functional P2X(7)-like receptor in cerebellar granule neurons from P2X(7) knockout mice. FEBS Lett 579(17):3783-8. [PubMed: 15978588]  [MGI Ref ID J:99778]

Sander LE; Davis MJ; Boekschoten MV; Amsen D; Dascher CC; Ryffel B; Swanson JA; Muller M; Blander JM. 2011. Detection of prokaryotic mRNA signifies microbial viability and promotes immunity. Nature 474(7351):385-9. [PubMed: 21602824]  [MGI Ref ID J:172745]

Shenderov K; Riteau N; Yip R; Mayer-Barber KD; Oland S; Hieny S; Fitzgerald P; Oberst A; Dillon CP; Green DR; Cerundolo V; Sher A. 2014. Cutting edge: Endoplasmic reticulum stress licenses macrophages to produce mature IL-1beta in response to TLR4 stimulation through a caspase-8- and TRIF-dependent pathway. J Immunol 192(5):2029-33. [PubMed: 24489101]  [MGI Ref ID J:209937]

Sim JA; Young MT; Sung HY; North RA; Surprenant A. 2004. Reanalysis of P2X7 receptor expression in rodent brain. J Neurosci 24(28):6307-14. [PubMed: 15254086]  [MGI Ref ID J:97270]

Sun S; Xia S; Ji Y; Kersten S; Qi L. 2012. The ATP-P2X7 signaling axis is dispensable for obesity-associated inflammasome activation in adipose tissue. Diabetes 61(6):1471-8. [PubMed: 22415881]  [MGI Ref ID J:196855]

Taylor SR; Gonzalez-Begne M; Dewhurst S; Chimini G; Higgins CF; Melvin JE; Elliott JI. 2008. Sequential shrinkage and swelling underlie P2X7-stimulated lymphocyte phosphatidylserine exposure and death. J Immunol 180(1):300-8. [PubMed: 18097031]  [MGI Ref ID J:130933]

Taylor SR; Gonzalez-Begne M; Sojka DK; Richardson JC; Sheardown SA; Harrison SM; Pusey CD; Tam FW; Elliott JI. 2009. Lymphocytes from P2X7-deficient mice exhibit enhanced P2X7 responses. J Leukoc Biol 85(6):978-86. [PubMed: 19276178]  [MGI Ref ID J:149750]

Toma C; Higa N; Koizumi Y; Nakasone N; Ogura Y; McCoy AJ; Franchi L; Uematsu S; Sagara J; Taniguchi S; Tsutsui H; Akira S; Tschopp J; Nunez G; Suzuki T. 2010. Pathogenic Vibrio activate NLRP3 inflammasome via cytotoxins and TLR/nucleotide-binding oligomerization domain-mediated NF-kappaB signaling. J Immunol 184(9):5287-97. [PubMed: 20348425]  [MGI Ref ID J:160469]

Tourneur L; Mistou S; Schmitt A; Chiocchia G. 2008. Adenosine receptors control a new pathway of Fas-associated death domain protein expression regulation by secretion. J Biol Chem 283(26):17929-38. [PubMed: 18445587]  [MGI Ref ID J:138177]

Traves PG; Pimentel-Santillana M; Carrasquero LM; Perez-Sen R; Delicado EG; Luque A; Izquierdo M; Martin-Sanz P; Miras-Portugal MT; Bosca L. 2013. Selective Impairment of P2Y Signaling by Prostaglandin E2 in Macrophages: Implications for Ca2+-Dependent Responses. J Immunol 190(8):4226-35. [PubMed: 23479225]  [MGI Ref ID J:194901]

Vergani A; Fotino C; D'Addio F; Tezza S; Podetta M; Gatti F; Chin M; Bassi R; Molano RD; Corradi D; Gatti R; Ferrero ME; Secchi A; Grassi F; Ricordi C; Sayegh MH; Maffi P; Pileggi A; Fiorina P. 2013. Effect of the purinergic inhibitor oxidized ATP in a model of islet allograft rejection. Diabetes 62(5):1665-75. [PubMed: 23315496]  [MGI Ref ID J:208579]

Vessey KA; Fletcher EL. 2012. Rod and cone pathway signalling is altered in the P2X7 receptor knock out mouse. PLoS One 7(1):e29990. [PubMed: 22253851]  [MGI Ref ID J:184314]

Weber FC; Esser PR; Muller T; Ganesan J; Pellegatti P; Simon MM; Zeiser R; Idzko M; Jakob T; Martin SF. 2010. Lack of the purinergic receptor P2X(7) results in resistance to contact hypersensitivity. J Exp Med 207(12):2609-19. [PubMed: 21059855]  [MGI Ref ID J:176873]

Wen H; Gris D; Lei Y; Jha S; Zhang L; Huang MT; Brickey WJ; Ting JP. 2011. Fatty acid-induced NLRP3-ASC inflammasome activation interferes with insulin signaling. Nat Immunol 12(5):408-15. [PubMed: 21478880]  [MGI Ref ID J:172610]

Wilhelm K; Ganesan J; Muller T; Durr C; Grimm M; Beilhack A; Krempl CD; Sorichter S; Gerlach UV; Juttner E; Zerweck A; Gartner F; Pellegatti P; Di Virgilio F; Ferrari D; Kambham N; Fisch P; Finke J; Idzko M; Zeiser R. 2010. Graft-versus-host disease is enhanced by extracellular ATP activating P2X7R. Nat Med 16(12):1434-8. [PubMed: 21102458]  [MGI Ref ID J:167519]

Witting A; Chen L; Cudaback E; Straiker A; Walter L; Rickman B; Moller T; Brosnan C; Stella N. 2006. Experimental autoimmune encephalomyelitis disrupts endocannabinoid-mediated neuroprotection. Proc Natl Acad Sci U S A 103(16):6362-7. [PubMed: 16571660]  [MGI Ref ID J:109031]

Woods LT; Camden JM; Batek JM; Petris MJ; Erb L; Weisman GA. 2012. P2X7 receptor activation induces inflammatory responses in salivary gland epithelium. Am J Physiol Cell Physiol 303(7):C790-801. [PubMed: 22875784]  [MGI Ref ID J:192784]

Zhou R; Tardivel A; Thorens B; Choi I; Tschopp J. 2010. Thioredoxin-interacting protein links oxidative stress to inflammasome activation. Nat Immunol 11(2):136-40. [PubMed: 20023662]  [MGI Ref ID J:158410]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as homozygotes

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

General Supply Notes

Control Information

  Control
   Wild-type from the colony
   001976 NOD/ShiLtJ (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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JAX® Mice
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Tel: 1-800-422-6423 or 1-207-288-5845
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Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

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phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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