Strain Name:

STOCK Rims1tm3Sud/J

Stock Number:

015832

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These Rims1 (regulating synaptic membrane exocytosis 1) floxed mice may be useful for generating animals lacking tissue-specific expression of the targeted gene.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
GenerationF?pN1
Generation Definitions
 
Donating InvestigatorDr. Thomas C. Sudhof,   Stanford University School of Medicine

Description
These mice possess loxP sites on either side of exon 6 in the Rims1 (regulating synaptic membrane exocytosis 1; Rim1α/β) gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.

Development
A targeting construct was designed to carry a loxP-flanked exon 6 into which a serine 413 to alanine (S413A) point mutation had been introduced. The floxed segment was immediately followed by an FRT-flanked drug resistance cassette including two copies of neomycin in intron 6. The construct was introduced to (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. An ES cell clone in which the point mutation was repaired by a known DNA repair mechanism was selected to produce chimeric mice. The neomycin selection cassette was excised from chimeric animals though crosses with an Actb beta actin promoter Flp recombinase mouse originally made on a B6SJLF2 background, and possibly backcrossed to C57BL/6NCrl. This left exon 6 flanked by loxP sites. This strain was maintained on a predominantly C57BL/6 and 129 mixed background by the donating laboratory.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Rims1
006384   B6;129-Rims1tm2Sud/J
006376   B6;129P2-Rims1tm1Sud/J
View Strains carrying other alleles of Rims1     (2 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Cone-Rod Dystrophy 7; CORD7   (RIMS1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Rims1tm3Sud/Rims1tm3Sud

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL   (conditional)
  • nervous system phenotype
  • abnormal inhibitory postsynaptic currents
    • hippocampal neurons treated actually or chronically with a cre-expressing lentivirus exhibit a reduced single evoked inhibitory post-synaptic current compared to in wild-type mice   (MGI Ref ID J:143053)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Cre-lox System
      loxP-flanked Sequences

Research Tools
Cre-lox System
      loxP-flanked Sequences
Neurobiology Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Rims1tm3Sud
Allele Name targeted mutation 3, Thomas C Sudhof
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) RIM1alphabetafloxed; fRIM1;
Mutation Made ByDr. Thomas Sudhof,   Stanford University School of Medicine
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Rims1, regulating synaptic membrane exocytosis 1
Chromosome 1
Gene Common Name(s) C030033M19Rik; CORD7; RAB3IP2; RIKEN cDNA C030033M19 gene; RIM; RIM1; RIM1a; RIM1alpha;
Molecular Note A loxP site was inserted upstream of exon 6 and an frt-flanked double neo cassette with a 5' loxP site was inserted downstream of exon 6. The neo cassette was removed by germ line, flp mediated recombination leaving exon 6 floxed. [MGI Ref ID J:143053]

Genotyping

Genotyping Information

Genotyping Protocols

Rims1tm3Sudalternate1, Standard PCR
Rims1tm3Sud, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Kaeser PS; Kwon HB; Chiu CQ; Deng L; Castillo PE; Sudhof TC. 2008. RIM1alpha and RIM1beta are synthesized from distinct promoters of the RIM1 gene to mediate differential but overlapping synaptic functions. J Neurosci 28(50):13435-47. [PubMed: 19074017]  [MGI Ref ID J:143053]

Additional References

Rims1tm3Sud related

Haws ME; Kaeser PS; Jarvis DL; Sudhof TC; Powell CM. 2012. Region-specific deletions of RIM1 reproduce a subset of global RIM1alpha(-/-) phenotypes. Genes Brain Behav 11(2):201-13. [PubMed: 22103334]  [MGI Ref ID J:181676]

Kaeser PS; Deng L; Fan M; Sudhof TC. 2012. RIM genes differentially contribute to organizing presynaptic release sites. Proc Natl Acad Sci U S A 109(29):11830-5. [PubMed: 22753485]  [MGI Ref ID J:186493]

Kaeser PS; Deng L; Wang Y; Dulubova I; Liu X; Rizo J; Sudhof TC. 2011. RIM proteins tether Ca2+ channels to presynaptic active zones via a direct PDZ-domain interaction. Cell 144(2):282-95. [PubMed: 21241895]  [MGI Ref ID J:168840]

Narboux-Neme N; Evrard A; Ferezou I; Erzurumlu RS; Kaeser PS; Laine J; Rossier J; Ropert N; Sudhof TC; Gaspar P. 2012. Neurotransmitter release at the thalamocortical synapse instructs barrel formation but not axon patterning in the somatosensory cortex. J Neurosci 32(18):6183-96. [PubMed: 22553025]  [MGI Ref ID J:184862]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, homozygotes or heterozygotes may be bred.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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