Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Wild-type x Heterozygote         (Female x Male)   23-APR-12 Mating System Heterozygote x Wild-type         (Female x Male)   23-APR-12 Species laboratory mouse   Donating Investigator Richard M. Locksley,   Univ of California San Francisco-HHMI

Description
A targeting vector was designed to insert an internal ribosome entry site (IRES)-enhanced yellow fluorescent protein (eYFP) fusion protein, downstream of the endogenous stop codon of the arginase (Arg1) gene. These homozygous YARG mice are viable, fertile, and normal in size. Located in the cytoplasm of the liver and specifically in macrophages, arginase I converts L-arginine into L-ornithine and urea as the final step in the urea cycle. L-arginine can also be catabolized into Nitric oxide (NO), a secreted free radical which causes tissues damage and is also toxic to bacteria. Arginase I suppresses NO production by decreasing the amount of L-arginine available to the nitric oxide synthase (NOS) enzyme for conversion into NO and other reactive oxygen species (ROS). Nine days after infection with the helminth, Nippostrongylus brasiliensis, YARG mice exhibit accumulation of arginase I-expressing macrophages. As soon as two days after inoculation with chitin, a polysaccharide found in the exoskeleton of some invertebrates, these macrophages were present in the lung and peritoneum. These mice may be useful for visualization of arginase-containing cells and their response to infection.

Development
A targeting vector was designed to insert a floxed neomycin (neo) resistance cassette, followed by an internal ribosome entry site (IRES)-enhanced yellow fluorescent protein (eYFP) fusion protein, downstream of the endogenous stop codon of the arginase (Arg) gene. The construct was electroporated into 129S4/SvJae-derived PC3 embryonic stem (ES) cells. These PC3 cells contain a transgene expressing Cre recombinase under control of the protamine promoter (Tg(Prm-cre)700g), to delete the neo cassette in the male germline. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6 females. The male offspring from this initial breeding lack the floxed neo cassette and were bred to female C57BL/6 mice to establish a colony of YARG mice. These mutant mice were backcrossed to BALB/cJ mice for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to BALB/cJ (Stock No. 000651) for at least one generation.

Control Information

	Control
	Wild-type from the colony
	000651 BALB/cJ	(approximate)
Considerations for Choosing Controls

Related Strains

Strains carrying   Arg1^tm1Lky allele

015857

B6.129S4-Arg1tm1Lky/J

View Strains carrying   Arg1^tm1Lky     (1 strain)

Strains carrying other alleles of Arg1

007741	B6.129-Arg1tm1Rki/J
008879	C.B6-Arg1tm1Pmu/J
008817	C57BL/6-Arg1tm1Pmu/J

View Strains carrying other alleles of Arg1     (3 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Argininemia   (ARG1)

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Arg1^tm1Lky/Arg1^tm1Lky

        involves: 129S4/SvJae * C57BL/6

• normal phenotype
• no abnormal phenotype detected   (MGI Ref ID J:122735)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Signal Transduction

Metabolism Research
Free Radical Research

Research Tools
Genetics Research
      Tissue/Cell Markers
Immunology and Inflammation Research
Toxicology Research
      free radical research

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Arg1tm1Lky
Allele Name		targeted mutation 1, Richard M Locksley
Allele Type		Targeted (Reporter)
Common Name(s)		YARG;
Mutation Made By		Richard Locksley,   Univ of California San Francisco-HHMI
Strain of Origin		129S4/SvJae-Tg(Prm-cre)70Og
Gene Symbol and Name		Arg1, arginase, liver
Chromosome		10
Gene Common Name(s)		AI; AI256583; Arg-1; PGIF; expressed sequence AI256583;
Molecular Note		An IRES element linked to an enhanced YFP construct was inserted in exon 8 downstream of the endogenous stop codon and a floxed neomycin cassette. The neomycin selection cassette was excised in male mice by cre expressed from the Prm1 promoter. [MGI Ref ID J:122735]

Genotyping

Genotyping Information

Genotyping Protocols

Arg1^tm1Lky,

Separated MCA

Arg1^tm1Lky, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Reese TA; Liang HE; Tager AM; Luster AD; Van Rooijen N; Voehringer D; Locksley RM. 2007. Chitin induces accumulation in tissue of innate immune cells associated with allergy. Nature 447(7140):92-6. [PubMed: 17450126]  [MGI Ref ID J:122735]

Additional References

Arg1^tm1Lky related

Wu D; Molofsky AB; Liang HE; Ricardo-Gonzalez RR; Jouihan HA; Bando JK; Chawla A; Locksley RM. 2011. Eosinophils sustain adipose alternatively activated macrophages associated with glucose homeostasis. Science 332(6026):243-7. [PubMed: 21436399]  [MGI Ref ID J:170307]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous mice may be bred together.
Mating System	Wild-type x Heterozygote         (Female x Male)   23-APR-12
	Heterozygote x Wild-type         (Female x Male)   23-APR-12

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	Wild-type from the colony
	000651 BALB/cJ	(approximate)
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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