Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names STOCK Il4/Il13tm1.1Lky/J    (Changed: 20-OCT-11 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation +pN1 Generation Definitions   Donating Investigator Richard M. Locksley,   Univ of California San Francisco-HHMI

Description
These Il4/Il13^flox mutant mice possess a loxP site upstream of exon 3 of the interleukin 13 (Il13) gene and a second loxP site downstream of exon 4 of the interleukin 4 (Il4) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have nucleotides between exons 3 of Il13 and exon 4 of Il4 deleted in the cre-expressing tissue. This strain may be useful for studying immune function in mice lacking Il4 and Il13.

Development
A targeting vector was designed to insert a loxP-flanked blasticidin (bsd) resistance cassette upstream of exon 3 of the interleukin 13 (Il13) gene, and a frt-flanked neomycin resistance cassette followed by a single loxP site downstream of exon 4 of the interleukin 4 (Il4) gene. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a pMC-CreN expression plasmid to delete the bsd cassette. Resulting ES cells contained multiple gene rearrangments; intact floxed-exons, intact floxed-bsd cassette, or excision of both the exons and the bsd cassette. Correctly targeted ES cells, containing only the floxed-exons, were injected into C57BL/6 blastocysts and resulting chimeric males were bred with C57BL/6 females. Mice heterozygous for the Il4/Il13^flox allele were bred to mice containing Gt(ROSA)26Sor^tm1(FLP1)Dym to delete the neo cassette. This line was backcrossed to BALB/c mice for at least 10 generations. Upon arrival at The Jackson Laboratory Repository, mice were bred to BALB/cJ inbred mice (Stock No. 000651) for at least one generation.

Control Information

	Control
	Wild-type from the colony
	000651 BALB/cJ
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Il13

017353	C.129S4(B6)-Il13tm1(YFP/cre)Lky/J
018869	C.129S4(Cg)-Il13tm2.1Lky/J

View Strains carrying other alleles of Il13     (2 strains)

Strains carrying other alleles of Il4

002253	B6.129P2-Il4tm1Cgn/J
002496	BALB/c-Il4tm2Nnt/J
004190	C.129-Il4tm1Lky/J
003480	C.129S2(B6)-Il4tm1Gru/J
002518	C57BL/6-Il4tm1Nnt/J
002230	C57BL/6J-Tg(LckIl4)1315Dbl/J
005879	D1Lac.Cg-Il4tm1Cgn/J
002574	NOD.129P2(B6)-Il4tm1Cgn/Dvs
004222	NOD.129P2(B6)-Il4tm1Cgn/DvsJ
004291	NOD.Cg-Il10tm1Cgn Il4tm1Cgn/DvsJ
006698	NOD.Cg-Il4tm1Lky/JbsJ

View Strains carrying other alleles of Il4     (11 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Allergic Rhinitis   (IL13) Asthma, Susceptibility to   (IL13)

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Il4/Il13^tm1.1Lky/Il13⁺

        involves: 129P2/OlaHsd * 129S4/SvJaeSor

• no phenotypic analysis
• *normal* no phenotypic analysis   (MGI Ref ID J:157129)

Il4/Il13^tm1.1Lky/Il4/Il13^tm1.1Lky Il4/Il13^tm1.1Lky/Il4/Il13^tm1.1Lky

        involves: 129P2/OlaHsd * BALB/c

• homeostasis/metabolism phenotype
• abnormal noradrenaline level
  • noradrenaline content of adipose tissue is reduced 50-60% with cold exposure   (MGI Ref ID J:178294)
• decreased core body temperature
  • drops when exposed to 4C   (MGI Ref ID J:178294)
  • core temperature improves when treated with a beta adrenergic agonist   (MGI Ref ID J:178294)
• decreased fatty acid level
  • reduced circulating free fatty acids when exposed to 4C   (MGI Ref ID J:178294)
• growth/size phenotype
• increased susceptibility to weight loss
  • mice lose weight during cold exposure   (MGI Ref ID J:178294)

Il4/Il13^tm1.1Lky/Il4⁺

        involves: 129P2/OlaHsd * 129S4/SvJaeSor

• no phenotypic analysis
• *normal* no phenotypic analysis   (MGI Ref ID J:157129)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency
      multiple immune defects

Neurobiology Research
Cre-lox System
      loxP-flanked Sequences

Research Tools
Cre-lox System
      loxP-flanked Sequences
      loxP-flanked Sequences: Test/Reporter

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Il4/Il13tm1.1Lky
Allele Name		targeted mutation 1.1, Richard M Locksley
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		4-13F; Il4-IL13fl;
Strain of Origin		129P2/OlaHsd
Gene Symbol and Name		Il4, interleukin 4
Chromosome		11
Gene Common Name(s)		BCGF-1; BCGF1; BSF-1; BSF1; IL-4; Il-4; Il4e12;
Molecular Note		A single targeting event was used to insert a floxed Bsd resistance cassette between exon 2 and 3 of Il13 and an frt flanked neo cassette with a 3' loxP site downstream of exon 4 of Il4 . Flp and cre mediated recombination removed the selection cassettes leaving the region between exon 3 of Il13 and exon 4 of Il4 floxed. [MGI Ref ID J:157129]
Gene Symbol and Name		Il13, interleukin 13
Chromosome		11
Gene Common Name(s)		IL-13; Il-13; P600;

Genotyping

Genotyping Information

Genotyping Protocols

Il4/Il13^tm1.1Lky, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Voehringer D; Wu D; Liang HE; Locksley RM. 2009. Efficient generation of long-distance conditional alleles using recombineering and a dual selection strategy in replicate plates. BMC Biotechnol 9:69. [PubMed: 19638212]  [MGI Ref ID J:157129]

Additional References

Il4/Il13^tm1.1Lky related

Nguyen KD; Qiu Y; Cui X; Goh YP; Mwangi J; David T; Mukundan L; Brombacher F; Locksley RM; Chawla A. 2011. Alternatively activated macrophages produce catecholamines to sustain adaptive thermogenesis. Nature 480(7375):104-8. [PubMed: 22101429]  [MGI Ref ID J:178294]

Sullivan BM; Liang HE; Bando JK; Wu D; Cheng LE; McKerrow JK; Allen CD; Locksley RM. 2011. Genetic analysis of basophil function in vivo. Nat Immunol 12(6):527-35. [PubMed: 21552267]  [MGI Ref ID J:172122]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous mice may be bred together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	Wild-type from the colony
	000651 BALB/cJ
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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