Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N5+pN1 Generation Definitions   Donating Investigator Richard M. Locksley,   Univ of California San Francisco-HHMI

Description
These PGRP-S mice contain a targeting vector designed to insert an enhanced yellow fluorescent protein (EYFP) immediately downstream from the initiation sequence of the peptidoglycan recognition protein, 1 (Pglyrp1) gene, abolishing gene expression. Homozygous mice are viable, fertile, normal in size and do not display any gross physical abnormalities. PGRP-S, belongs to a family of innate immune recognition molecules, and recognizes peptidoglycan components of bacterial cell walls. Expressed mainly in neutrophil tertiary granules, PGRP-S is secreted and multimerizes in the serum. These PGRP-S-/- mice have normal inflammatory responses and production of tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6). PGRP-S knockout mice have increased susceptibility to intraperitoneal infection with gram-positive bacteria of low pathogenicity, but not with more pathogenic gram-positive or gram-negative bacteria. Neutrophils from PGRP-S-/- mice have normal phagocytic uptake of bacteria but are defective in intracellular killing and digestion of relatively nonpathogenic gram-positive bacteria. These mice may be useful for visualizing PGRP-S expressing cells and for studying the role of peptidoglycan recognition proteins in immune function.

Development
A targeting vector was designed to insert an enhanced yellow fluorescent protein (EYFP), followed by a floxed neomycin (neo) resistance cassette, immediately downstream from the initiation sequence of the peptidoglycan recognition protein, 1 (Pglyrp1) gene. The construct was electroporated into 129S4/SvJae-derived PC3 embryonic stem (ES) cells. These PC3 cells contain a transgene expressing Cre recombinase under control of the protamine promoter (Tg(Prm-cre)700g), to delete the neo cassette in the male germline. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6 females. The male offspring from this initial breeding lack the floxed neo cassette and were bred to female C57BL/6 mice to establish a colony. These PGRP-S mutant mice were backcrossed to C57BL/6 mice for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation.

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls

Additional Web Information

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Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Pglyrp1^tm1.1Lky/Pglyrp1^tm1.1Lky

        B6.129S4-Pglyrp1^tm1.1Lky

• immune system phenotype
• abnormal neutrophil physiology
  • neutrophils exhibit defective intracellular killing and digestion of relatively nonpathogenic gram-positive bacteria compared with wild-type cells   (MGI Ref ID J:171412)
  • however, neutrophil phagocytic uptake is normal   (MGI Ref ID J:171412)
• increased susceptibility to bacterial infection
  • following intraperitoneal infection with gram-positive bacteria of low pathogenicity   (MGI Ref ID J:171412)
  • however, susceptibility to infection with more pathogenic gram-positive or gram-negative bacteria is normal   (MGI Ref ID J:171412)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Hematological Research
Immunological Defects

Research Tools
Genetics Research
      Tissue/Cell Markers

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Pglyrp1tm1.1Lky
Allele Name		targeted mutation 1.1, Richard M Locksley
Allele Type		Targeted (Reporter)
Common Name(s)		PGRP-S;
Strain of Origin		129S4/SvJae-Tg(Prm-cre)70Og
Gene Symbol and Name		Pglyrp1, peptidoglycan recognition protein 1
Chromosome		7
Gene Common Name(s)		PGLYRP; PGRP; PGRP-S; PGRPS; TAG7; TNFSF3L; Tasg7; Tnfsf3l; peptidoglycan recognition protein; tumor necrosis factor super family 3-like; tumor-associated gene 7;
Molecular Note		A targeting vector was designed to insert an enhanced yellow fluorescent protein (EYFP), followed by a floxed neomycin (neo) resistance cassette, immediately downstream from the initiation sequence of the peptidoglycan recognition protein, 1 (Pglyrp1) gene. Cre-mediated recombination removed the neo cassette. [MGI Ref ID J:171412]

Genotyping

Genotyping Information

Genotyping Protocols

Pglyrp1^tm1.1Lky, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Locksley RM. 2011. Direct Data Submission 2011/05/10 MGI Direct Data Submission :.  [MGI Ref ID J:171412]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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