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| A targeting vector containing β-galactosidase, a polyadenylation signal, and a PGK Hygromycin selection cassette, was randomly inserted downstream of exon 1 of the endogenous G protein-coupled receptor kinase-interactor 1 (Git1) gene. These mice may be useful as a lacZ reporter for Git1 expression. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Gene Trap; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N10pN1
Generation DefinitionsDonating Investigator Dr. Arthur Weiss, University of California, San Francisco Description
A targeting vector containing β-galactosidase, a polyadenylation signal, and a PGK Hygromycin selection cassette, was randomly inserted downstream of exon 1 of the endogenous G protein-coupled receptor kinase-interactor 1 (Git1) gene. Heterozygous mice are viable, normal in size and do not display any gross physical or behavioral abnormalities, although some males have decreased fertility. Homozygous mice die within a few days after birth. This β-geo secretory trap mutation abolishes endogenous gene function and expresses a Git1-exon1/lacZ fusion protein. Git1 belongs to the family of ADP-ribosylation factor GTPase-activating proteins (ARF-GAP) and has been implicated in the regulation of G protein-coupled receptor sequestration, cell migration, neuronal spine formation, and aggregate formation in Huntington's disease. GIT1 expression restricted to some areas of the brain, to cells lining blood vessels, bronchi, and bile ducts. Expression of GIT1 is absent during spermatid differentiation, but is present in mature spermatids and in mature ovarian follicles. These mice may be useful as a lacZ reporter for Git1 expression.Development
A "secretory trap" targeting vector, containing β-galactosidase, a polyadenylation signal, and a PGK hygromycin selection cassette, was electroporated into 129S4/SvJaeSor-derived AK7.1 embryonic stem (ES) cells. This vector inserted downstream of exon 1 of the endogenous G protein-coupled receptor kinase-interactor 1 (Git1) gene. Correctly targeted ES cells were injected into C57BL/6 blastocysts, and chimeric mice were bred with C57BL/6 mice. These Git1(S10-12C(P17-1B) mice were backcrossed to BALB/c for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to BALB/cJ (Stock No. 000651) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000651 BALB/cJ | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (255 strains)
016092 B6.129S4-Git1Gt(FHCRC-GT-S10-12C1)Sor/WeisJ
Additional Web Information
Fluorescent Proteins/lacZ Systems
Use of MICE by companies or for-profit entities requires a license prior to shipping.
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Git1Gt(FHCRC-GT-S10-12C1)Sor/Git1+
involves: 129S4/SvJae * C57BL/6
- normal phenotype
- no abnormal phenotype detected
- mice are normal with no differences in body size, organ size, overt behavior, or fertility when compared to wild-type mice (MGI Ref ID J:126878)
Git1Gt(FHCRC-GT-S10-12C1)Sor/Git1Gt(FHCRC-GT-S10-12C1)Sor
involves: 129S4/SvJaeSor * C57BL/6
- mortality/aging
- partial postnatal lethality
- 50% of mice die postnatally (MGI Ref ID J:171608)
- behavior/neurological phenotype
- abnormal object recognition memory
- abnormal spatial learning
- in a Morris water maze, mice exhibit impaired learning compared with wild-type mice (MGI Ref ID J:171608)
- hyperactivity
- impaired swimming
- mice exhibit reduced swimming velocity compared with wild-type mice (MGI Ref ID J:171608)
- nervous system phenotype
- abnormal CNS synaptic transmission
- abnormal brain wave pattern
- decreased neuron number
- the number of parvalbumin neurons in the hippocampal area and cortex is reduced compared to in wild-type mice (MGI Ref ID J:171608)
- growth/size phenotype
- decreased body weight
- in surviving mice (MGI Ref ID J:171608)
This mouse can be used to support research in many areas including:
Cell Biology Research
Cell Motility Defects
Developmental Biology Research
Postnatal Lethality
Homozygous
Neurobiology Research
lacZ expression in neural tissue
Huntington's disease
Reproductive Biology Research
Fertility Defects
males only
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Git1Gt(FHCRC-GT-S10-12C1)Sor | ||
|---|---|---|---|
| Allele Name | gene trap FHCRC-GT-S10-12C1, Philippe Soriano | ||
| Allele Type | Gene trapped | ||
| Common Name(s) | GIT1-; | ||
| Mutation Made By | Dr. Arthur Weiss, University of California, San Francisco | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| Site of Expression | Git1 expression is restricted to some areas of the brain, to cells lining blood vessels, bronchi, and bile ducts. Expression of Git1 is absent in differentiating spermatids, but is present in mature spermatids and in mature ovarian follicles. | ||
| Gene Symbol and Name | Git1, G protein-coupled receptor kinase-interactor 1 | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | Cat-1; p95Cat; | ||
| Molecular Note | The gene trap vector containing a beta-geo cassette and a PGK-hygro cassette was inserted into intron 1. Insertion was confirmed by genomic PCR and expression of the lacZ cassette was confirmed by beta-galactosidase staining. [MGI Ref ID J:126878] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Git1Gt(FHCRC-GT-S10-12C1)Sor, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Schmalzigaug R; Phee H; Davidson CE; Weiss A; Premont RT. 2007. Differential expression of the ARF GAP genes GIT1 and GIT2 in mouse tissues. J Histochem Cytochem 55(10):1039-48. [PubMed: 17565117] [MGI Ref ID J:126878]
Additional References
Git1Gt(FHCRC-GT-S10-12C1)Sor relatedWon H; Mah W; Kim E; Kim JW; Hahm EK; Kim MH; Cho S; Kim J; Jang H; Cho SC; Kim BN; Shin MS; Seo J; Jeong J; Choi SY; Kim D; Kang C; Kim E. 2011. GIT1 is associated with ADHD in humans and ADHD-like behaviors in mice. Nat Med 17(5):566-72. [PubMed: 21499268] [MGI Ref ID J:171608]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, heterozygous mice are bred to wildtype siblings.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000651 BALB/cJ | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
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JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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