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| A targeting vector containing β-galactosidase was randomly inserted downstream of exon 2 of the endogenous G protein-coupled receptor kinase-interactor 2 (Git2) gene. These mice may be useful as a lacZ reporter for Git2 expression. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Gene Trap; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N10pN1
Generation DefinitionsDonating Investigator Dr. Arthur Weiss, University of California, San Francisco Description
A targeting vector containing β-galactosidase was randomly inserted downstream of exon 2 of the endogenous G protein-coupled receptor kinase-interactor 2 (Git2) gene. Homozygous mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This β-geo secretory trap mutation abolishes endogenous gene function and expresses a Git2-exon1-2/lacZ fusion protein. Git2 belongs to the family of ADP-ribosylation factor GTPase-activating proteins (ARF-GAP) and has been implicated in the regulation of G protein-coupled receptor sequestration, cell migration, T-cell activation, neuronal spine formation, and aggregate formation in Huntington's disease. GIT2 expression is seen throughout the liver, lungs, spleen, muscle, and the central nervous system. Expression of GIT2 is also seen during absent spermatid differentiation but is absent in mature spermatids. These mice may be useful as a lacZ reporter for Git2 expression.Development
A "secretory trap" targeting vector containing β-galactosidase was electroporated into 129P2/OlaHsd-derived E14TG2a.4 embryonic stem (ES) cells. This vector inserted downstream of exon 2 of the endogenous G protein-coupled receptor kinase-interactor 2 (Git2) gene. Correctly targeted ES cells were injected into C57BL/6 blastocysts, and chimeric mice were bred with C57BL/6 mice. These Git2(XG510) mice were backcrossed to BALB/c for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to BALB/cJ (Stock No. 000651) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| 000651 BALB/cJ | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Huntington's Disease Models
007708 B6.129-Gt(ROSA)26Sortm1(HD*103Q)Xwy/J 003454 B6.129-Htttm3Mem/J 003597 B6.129-Htttm4Mem/J 003598 B6.129-Htttm5Mem/J 016094 B6.129P2-Git2Gt(XG510)Byg/WeisJ 016522 B6.129P2-Htttm2Detl/100J 004595 B6.129P2-Htttm2Detl/150J 016521 B6.129P2-Htttm2Detl/50J 002688 B6.129S4-Htttm1Mem/J 006471 B6.Cg-Tg(HDexon1)61Gpb/J 008333 B6;129P2-Dldtm1Ptl/J 004360 B6;SJL-Tg(HD)63Aron/J 003627 B6C3-Tg(HD82Gln)81Dbo/J 002809 B6CBA-Tg(HDexon1)61Gpb/1J 002810 B6CBA-Tg(HDexon1)62Gpb/1J 006494 B6CBA-Tg(HDexon1)62Gpb/3J 008833 C57BL/6-Tg(Camk2a-UBB)3413-1Fwvl/J 007578 CBy.Cg-Tg(HDexon1)61Gpb/J 013732 FVB-Tg(NPEPPS)1Skar/J 004938 FVB-Tg(YAC128)53Hay/J 012630 FVB/N-Tg(GFAP-HTT*160Q)31Xjl/J 008197 FVB/N-Tg(HTT*97Q)IXwy/J 007247 FVB/N-Tg(YAC353G6)W7Hay/J 003640 FVB/NJ-Tg(YAC72)2511Hay/J 007749 STOCK Hap1tm1Xjl/J 003453 STOCK Htttm2Mem/J 003455 STOCK Htttm4Mem/J 003456 STOCK Htttm5Mem/J View Huntington's Disease Models (28 strains)
016094 B6.129P2-Git2Gt(XG510)Byg/WeisJ
Additional Web Information
Fluorescent Proteins/lacZ Systems
Visit the Huntington's Diease Resource site for helpful information on Huntington's Disease and research resources.
Phenotype
Phenotype Information
assigned by genotype
Git2Gt(XG510)Byg/Git2Gt(XG510)Byg
C.129P2-Git2Gt(XG510)Byg
- hematopoietic system phenotype
- decreased marginal zone B cell number (MGI Ref ID J:160613)
- immune system phenotype
- decreased marginal zone B cell number (MGI Ref ID J:160613)
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Git2Gt(XG510)Byg/Git2+
involves: 129P2/OlaHsd * C57BL/6
- normal phenotype
- no abnormal phenotype detected
- mice are normal with no differences in body size, organ size, overt behavior, or fertility when compared to wild-type mice (MGI Ref ID J:126878)
Git2Gt(XG510)Byg/Git2Gt(XG510)Byg
B6.129P2-Git2Gt(XG510)Byg
- hematopoietic system phenotype
- decreased marginal zone B cell number (MGI Ref ID J:160613)
- immune system phenotype
- decreased marginal zone B cell number (MGI Ref ID J:160613)
Git2Gt(XG510)Byg/Git2Gt(XG510)Byg
Background Not Specified
- behavior/neurological phenotype
- increased thermal nociceptive threshold (MGI Ref ID J:175295)
- hematopoietic system phenotype
- decreased leukocyte cell number (MGI Ref ID J:175295)
- homeostasis/metabolism phenotype
- decreased circulating magnesium level (MGI Ref ID J:175295)
- increased circulating HDL cholesterol level (MGI Ref ID J:175295)
- immune system phenotype
- decreased leukocyte cell number (MGI Ref ID J:175295)
- integument phenotype
- increased thermal nociceptive threshold (MGI Ref ID J:175295)
This mouse can be used to support research in many areas including:
Cell Biology Research
Cell Motility Defects
Neurobiology Research
lacZ expression in neural tissue
Huntington's disease
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Git2Gt(XG510)Byg | ||
|---|---|---|---|
| Allele Name | gene trap XG510, BayGenomics | ||
| Allele Type | Gene trapped | ||
| Common Name(s) | GIT2-; | ||
| Mutation Made By | Dr. Arthur Weiss, University of California, San Francisco | ||
| Strain of Origin | 129P2/OlaHsd | ||
| Site of Expression | Git2 expression is seen throughout the liver, lungs, spleen, muscle, and the central nervous system. Expression of Git2 is also seen during spermatid differentiation but is absent in mature spermatids. | ||
| Gene Symbol and Name | Git2, G protein-coupled receptor kinase-interactor 2 | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | 1500036H07Rik; 5830420E16Rik; 9630056M03Rik; ARF GTPase activating protein 2; B230104M05Rik; CAT-2; CAT2; Cool associated tyrosine phosphorylated-2; RIKEN cDNA 1500036H07 gene; RIKEN cDNA 5830420E16 gene; RIKEN cDNA 9630056M03 gene; RIKEN cDNA B230104M05 gene; | ||
| Molecular Note | A gene trap vetor containing a beta-geo cassette was inserted into intron 2. Insertion was confirmed by PCR genotyping and expression of the lacZ gene was confirmed by beta-galactosidase staining. Two transcripted were detected by RT-PCR, one the expected fusion of exon 1 and 2 to the gene trap vector sequence and the other an alternative transcript lacking exon 2. [MGI Ref ID J:126878] | ||
References
References provided by MGI
Selected Reference(s)
Schmalzigaug R; Phee H; Davidson CE; Weiss A; Premont RT. 2007. Differential expression of the ARF GAP genes GIT1 and GIT2 in mouse tissues. J Histochem Cytochem 55(10):1039-48. [PubMed: 17565117] [MGI Ref ID J:126878]
Additional References
Git2Gt(XG510)Byg relatedMouse Genome Informatics and the Wellcome Trust Sanger Institute Mouse Genetics Project (MGP). 2011. Obtaining and Loading Phenotype Annotations from the Wellcome Trust Sanger Institute (WTSI) Mouse Resources Portal Database Release :. [MGI Ref ID J:175295]
Phee H; Dzhagalov I; Mollenauer M; Wang Y; Irvine DJ; Robey E; Weiss A. 2010. Regulation of thymocyte positive selection and motility by GIT2. Nat Immunol 11(6):503-11. [PubMed: 20431621] [MGI Ref ID J:160613]
Schmalzigaug R; Rodriguiz RM; Phillips LE; Davidson CE; Wetsel WC; Premont RT. 2009. Anxiety-like behaviors in mice lacking GIT2. Neurosci Lett 451(2):156-61. [PubMed: 19114090] [MGI Ref ID J:146556]
Stryke D; Kawamoto M; Huang CC; Johns SJ; King LA; Harper CA; Meng EC; Lee RE; Yee A; L'Italien L; Chuang PT; Young SG; Skarnes WC; Babbitt PC; Ferrin TE. 2003. BayGenomics: a resource of insertional mutations in mouse embryonic stem cells. Nucleic Acids Res 31(1):278-81. [PubMed: 12520002] [MGI Ref ID J:91812]
Wang X; Liao S; Nelson ER; Schmalzigaug R; Spurney RF; Guilak F; Premont RT; Gesty-Palmer D. 2012. The cytoskeletal regulatory scaffold protein GIT2 modulates mesenchymal stem cell differentiation and osteoblastogenesis. Biochem Biophys Res Commun 425(2):407-12. [PubMed: 22846567] [MGI Ref ID J:187156]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| 000651 BALB/cJ | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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