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| These mice contain a reverse tetracycline-controlled transactivator (rtTA) protein in the 5' untranslated region of the whey acidic protein gene. Theses Wap-rtTA mice are useful in regulating the expression of proteins (including oncoproteins) in mammary epithelium. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F?pN1
Generation DefinitionsDonating Investigator Dr. Kay-Uwe Wagner, University of Nebraska Medical Center Description
These mice contain a reverse tetracycline-controlled transactivator (rtTA) protein in the 5' untranslated region of the whey acidic protein (Wap) gene. Homozygous mice are viable, fertile, and normal in size. WAP, expressed late during pregnancy and during lactation in the mammary gland, is the principal whey protein found in rodent milk. When mated to a mutant strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TetO), the Wap-rtTA allele mediates expression of TetO-responder genes in the developing mammary gland upon administration of doxycycline (dox). Since expression of the endogenous WAP locus is elevated during pregnancy and lactation, the expression of TetO responder genes is particularly high during these stages of mammary gland development. The Wap-rtTA mice are useful to express exogenous proteins including teto-regulated oncogenes in the mammary epithelium.Development
A targeting vector was designed to place a reverse tetracycline-controlled transactivator (rtTA) protein, followed by a loxP-flanked neomycin resistance (neo) cassette, in the 5' untranslated region of the whey acidic protein (Wap) gene. The construct was electroporated into 129X1/SvJ-derived RW-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL6NCrl blastocysts and the resulting chimeric males were bred to C57BL6NCrl females. Offspring were then crossed to STOCK-Tg(MMTV-Cre)1Mam/J transgenic mice (Stock No. 003551) to remove the neo cassette. The resulting double positive females were then bred to wildtype males to remove the Cre transgene. These mice were maintained on a mixed background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6NJ (Stock No. 005304) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| 005304 C57BL/6NJ | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Wap
010562 B6.Cg-Tg(Wap-ERBB2)229Wzw/J 008735 B6.Cg-Tg(Wap-cre)11738Mam/JKnwJ 003552 B6129-Tg(Wap-cre)11738Mam/J 002409 B6;SJL-Tg(Wap-HRAS)69Lln Chr YSJL/J 003189 C57BL/6J-Tg(WAPTAg)3Knw/J 002500 C57BL/6J-Tg(WapIgf1)39Dlr/J 003188 C57BL/6J-Tg(WapTAg)1Knw/J 002410 FVB.Cg-Tg(Wap-HRAS)69Lln Chr YSJL/J 003078 FVB.Cg-Tg(WapIgf1)39Dlr/J 002678 FVB.Cg-Tg(WapTgfa)215Bri/J 002755 FVB/N-Tg(WapNotch4)10Rnc/J 004184 STOCK Tg(Wap-HRAS)69Lln Chr YSJL-Edaraddcr-3J/J View Strains carrying other alleles of Wap (12 strains)
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Cell Biology Research
Protein Processing
Research Tools
Cancer Research
Tetop Tet System
Genetics Research
Mutagenesis and Transgenesis: Tetop Tet System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Waptm2(rtTA)Kuw | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Kay Uwe Wagner | ||
| Allele Type | Targeted (knock-in) | ||
| Mutation Made By | Dr. Kay-Uwe Wagner, University of Nebraska Medical Center | ||
| Strain of Origin | 129X1/SvJ | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| Molecular Note | The coding sequence for rtTA was inserted into the 5'-UTR along with a floxed neomycin selection cassette. The neo selection cassette was eliminated in founder line 7A3 by crossing properly targeted mice to Tg(MMTV-cre)1Mam transgenic animals. When micewere given doxycycline, transgene expression was restricted to mammary epithelial cells as determined by crosses with two different rtTA reporter strains. [MGI Ref ID J:148684] | ||
| Gene Symbol and Name | Wap, whey acidic protein | ||
| Chromosome | 11 | ||
Genotyping
Genotyping Information
Genotyping Protocols
Waptm2(rtTA)Kuw, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Creamer BA; Triplett AA; Wagner KU. 2009. Longitudinal analysis of mammogenesis using a novel tetracycline-inducible mouse model and in vivo imaging. Genesis 47(4):234-45. [PubMed: 19208431] [MGI Ref ID J:148684]
Additional References
Waptm2(rtTA)Kuw relatedCreamer BA; Sakamoto K; Schmidt JW; Triplett AA; Moriggl R; Wagner KU. 2010. Stat5 promotes survival of mammary epithelial cells through transcriptional activation of a distinct promoter in Akt1. Mol Cell Biol 30(12):2957-70. [PubMed: 20385773] [MGI Ref ID J:162571]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2085.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2710.50 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| 005304 C57BL/6NJ | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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For Licensing and Use Restrictions view the link(s) below:
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Contact information
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| phone: | 207-288-6470 |
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JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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